Sugarcane tops silage (STS), as a source of roughage for ruminants, is rich in water-soluble carbohydrate (WSC) content, which significantly affects silage quality. Citric acid (CA) is a low-cost natural antimicrobial agent that can inhibit undesirable microbes and improve silage quality. The objectives of this study were to investigate the effects of CA on the chemical composition, fermentation quality, microbial communities, and metabolic pathways of STS with high and low WSC contents before or after aerobic exposure. Fresh sugarcane tops with low-WSC [143.05 g/kg dry matter (DM)] and high-WSC (249.99 g/kg DM) contents were treated with and without CA and then ensiled for 125 days, followed by aerobic exposure for 4, 8, and 16 days. The results showed that high-WSC STS had lower crude protein (CP) content and higher DM, neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents, whether treated with CA or not. CA-inoculated silage exhibited decreased DM loss and enterobacteria (EB) counts compared to the control. High-WSC STS treated with CA had higher WSC content and lower yeast count than those without CA inoculation. During the 0–16 days of aerobic exposure, the propionic acid and butyric acid contents in CA-inoculated silage were almost unchanged and ranged from 0 to 1 g/kg DM. Meanwhile, the ethanol content was almost unchanged and ranged from 0 to 1 g/kg DM only in low-WSC STS, irrespective of CA addition. Before aerobic exposure, CA inoculation decreased the abundances of undesirable microbes (e.g., Clostridium_sensu_stricto_12 and Paecilomyces) and animal pathogens, while amino acid metabolism was lower in high-WSC STS regardless of CA treatment. After aerobic exposure, CA inoculation increased the abundance of bacteria with antibacterial effects, including Paenibacillus and Bacillus. Moreover, the metabolism of energy and nucleotides was lower in high-WSC STS treated with CA, and the animal pathogens was lower in low-WSC STS treated with CA. In conclusion, CA inoculation could be effective in decreasing nutrients loss, improving fermentation quality, inhibiting harmful microorganisms, and modulating the metabolic pathways of microorganisms in STS with high and low WSC contents prior to and after aerobic exposure.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Abstract We examined the effect of supplemental dietary slow‐release urea on the growth performance and physiological status of 16 dairy Holstein heifers (10 months of age, 322 ± 10 kg). The heifers were offered a formulated isocaloric and isonitrogenous 70:30 roughage : concentrate ration and were assigned randomly to one of four levels of slow‐release urea supplementation (0% [U 0 ], 1% [U 1 ], 1.5% [U 1.5 ] and 2% [U 2 ] dry matter [ DM ]). The total study lasted 95 days, which included a 20 days adaptation period. Dry matter intake ( DMI ) of U 2 was lower than the intakes of U 0 and U 1 ( p < .05), while average daily gains ( ADG ) of U 1 and U 1.5 were higher than U 0 and U 2 ( p < .05). Rumen volatile fatty acids concentration did not differ among the four treatments, while ammonia nitrogen concentration increased with an increase in urea level ( p < .05). Serum blood urea nitrogen concentration was lower in U 1.5 than in U 0 and U 2 while serum free fatty acids concentration in U 2 was higher than in the other three treatments ( p < .05). We concluded that the addition of urea at a level of 1.5 to 2.0% DM resulted in a reduction in DMI but the addition of 1.0%–1.5% urea resulted in the highest ADG , with no negative effects on rumen fermentation and health status of the calves.
The soil microbial biomass carbon(MBC) and the ratio of microbial biomass carbon to soil organic carbon(qMB) were investigated in four different land-use types(Cinnamonum campora forest,Phyllostachys pubescens forest,Metasequoia glyptostroboides forest and cropland) in Xiaodian Lakes Forest Park which was formed by reclaiming from the Taihu lake,and also the impact of soil physical,chemical and biological properties on the MBC and qMB were analyzed.The results showed that soil MBC and qMB in cropland were significantly higher than that in C.campora,Ph.pubescens and M.glyptostroboides forests,respectively,and MBC in M.glyptostroboides forest was significantly lower than that in C.campora forest,Ph.pubescens forest.Soil MBC was strongly correlated with pH,total K content and soil respiration,while qMB was significantly correlated with soil moisture,TOC,TN,TK,C/N,pH,soil bulk density and soil respiration.The different land-use types will affect the changes of soil microbial biomass carbon after reclaiming from the Taihu lake.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Glacial lake ecosystems are experiencing rapid changes due to accelerated glacier retreat. As glaciers recede, their influence on downstream habitats diminishes, potentially affecting the biodiversity of glacial lake microbial communities. However, there remains a knowledge gap regarding how bacterial biodiversity patterns in glacial lakes are altered by diminishing glacial influence. Here, we investigated shifts in bacterial communities in paired water and sediment samples collected from seven glacial lakes on the Tibetan Plateau, using a space-for-time substitution approach to understand the consequences of glacier retreat. Our findings reveal that bacterial diversity in lake water increases significantly with a higher glacier index (GI), whereas sediment bacterial diversity exhibits a negative correlation with GI. Both the water and sediment bacterial communities display significant structural shifts along the GI gradient. Notably, reduced glacial influence decreases the complexity of bacterial co-occurrence networks in lake water but enhances the network complexity in sediment. This divergence in diversity and co-occurrence patterns highlights that water and sediment bacterial communities respond differently to changes in glacial influence in these lake ecosystems. This study provides insights into how diminishing glacial influence impacts the bacterial biodiversity in glacial lake water and sediments, revealing contrasting patterns between the two habitats. These findings emphasize the need for comprehensive monitoring to understand the implications of glacier retreat on these fragile ecosystems.
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