Objective To construct a cDNA subtractive library of genes transactivated by c-terminally truncated middle surface protein of hepatitis B virus(MHBs t) with suppression subtractive hybridization technique for cloning genes associated with transactivation. Methods The mRNA was isolated from HepG2 cells transfected with pcDNA3.1(-)-Mt167 and pcDNA3.1(-) empty vectors, respectively, then cDNA was synthesized. After restriction enzyme Rsa I digestion, small-size cDNAs were obtained. Then tester cDNA was divided into two groups and ligated to the specific adaptor 1 and adaptor 2, respectively. After tester cDNA was hybridized with driver cDNA twice and underwent two times of nested PCR and then was subcloned into T/A plasmid vectors to set up the subtractive library. Amplification of the library was carried out with E. coli strain JM109. The cDNA was sequenced and analyzed in GenBank with Blast search after PCR. Results The subtractive library of genes transactivated by MHBs t was constructed successfully. The amplified library contained 94 positive clones. Colony PCR showed that these clones contained 200-800bp inserts. Sequence analysis was performed in 50 clones,and the full length sequences were obtained with bioinformatics method. 23 coding sequences were obtained in total, which consisted of 19 known and 4 unknown ones.Conclusions The obtained sequences may be target genes transactivated by MHBs t, among which some genes coding proteins may involve in cell cycle regulation, immune response and tumour genesis.
Ustekinumab is a biological therapy that has been approved for treating moderate-to-severe psoriasis. Although injection site reactions, nasopharyngitis, headaches, and infections are the common adverse events associated with ustekinumab, the development of bullous pemphigoid (BP) is also thought to be related to ustekinumab. Given that psoriasis itself can be complicated by BP, it is worthwhile to investigate the relationship between ustekinumab, psoriasis, and BP. Here we report a case of a male patient who developed BP twice after psoriasis treatment with ustekinumab. The patient's psoriasis and BP were brought under control by discontinuing ustekinumab and administering methotrexate, minocycline, and topical corticosteroids. Because of the increasing use of biologics in patients with psoriasis, BP should be considered a potential adverse event associated with ustekinumab.
SIGNIFICANCE Machine learning random forest algorithms were used to predict objective refractive outcomes after cycloplegic refraction using noncycloplegic clinical data. A classification model predicted post-cycloplegic myopia and could be useful in screening, and a second regression model predicted post-cycloplegic refractive and could provide a useful objective starting point in noncycloplegic subjective refractions. PURPOSE A classification model sought to predict post-cycloplegic myopia using noncycloplegic clinical data to enhance myopia screening accuracy, whereas the regression model looked to predict objective refraction outcomes after cycloplegia for use as a starting point for noncycloplegic subjective refraction. METHODS A cross-sectional study included data from 2483 eyes. Pre-refraction measurements, such as uncorrected visual acuity, axial length, and corneal curvature radius, were recorded. After cycloplegia, the spherical equivalent was measured. Random forest–based classification and regression models were established with input variables including age, gender, axial length, corneal curvature radius, axial length–to–corneal curvature radius ratio, spherical equivalent, and uncorrected visual acuity. Model performance was assessed using various metrics. RESULTS The random forest classification model achieved high out-of-bag validation accuracy (92%), cross-validation accuracy (93%), external validation accuracy (94%), and precision (95%). The external validation sensitivity was 93%, and specificity was 95%. The regression model internal validation showed an out-of-bag validation R 2 of 0.86, root mean square error (RMSE) of 0.66, and mean absolute error of 0.49. The 10-fold cross-validation R 2 was 0.87, the RMSE was 0.64, and the mean absolute error was 0.48. In the external validation, R 2 was 0.88, the RMSE was 0.63, and the mean absolute error was 0.48. CONCLUSIONS By analyzing noncycloplegic clinical data, the classification model enables earlier detection of myopia, supporting timely intervention and management. The regression model aims to accurately predict post-cycloplegia myopic corrections, providing reliable initial data for subjective refraction. This could help optometrists perform noncycloplegic subjective refraction more efficiently and is particularly relevant in China, where retinoscopy is not yet fully popularized and many school students decline cycloplegic refraction due to academic pressures and limited free time, primarily because it requires a follow-up the next day.
patients had a decrease in HBV DNA > 2 log10 IU/mL in the vaccine group at week 12 after EOT compared with the control group.A trend toward a difference in the number of patients with undetectable HBV DNA at week 28 after EOT was obtained.Adverse events were similar.In the dynamic per-protocol set, which excluded adefovir (ADV) add-on cases at each time point instantly after ADV administration due to LAM antiviral failure, more patients had a decrease in HBV DNA > 2 log10 IU/mL in the vaccine group at week 12 and 28 after EOT compared with the control group.More patients with undetectable HBV DNA at week 28 after EOT in the vaccine group were also observed.Among patients with a viral load < 1000 copies/mL at week 12, more patients achieved HBeAg seroconversion in the vaccine group than among controls at week 36 after EOT, as well as less virological breakthrough and YMDD mutations. CONCLUSIONThe primary endpoint was not achieved using the HBV DNA vaccine.The HBV DNA vaccine could only be beneficial in subjects that have achieved initial virological response under LAM chemotherapy.
[Abstract] Objective To understand the damage to the cardiovascular systems of patients with different degrees of skeletal fluorosis after stopping fluorine exposure. Methods 65 patients with mild, moderate and severe skeletal fluorosis and 23 subjects without skeletal fluorosis as the control group were selected for detailed inquiry of cardiovascular disease history, blood pressure measurement and ECG examination. Results The rate of skeletal fluorosis patients with cardiovascular system symptoms accounted for 70.77%, and the difference was statistically significant as compared with that of the control group (P 0.05). [As seen from] the comparison of ECG parameters, the P-R intervals of the severe skeletal fluorosis group were significantly prolonged and statistically different as compared with those of the control group (P 0.05). Conclusion The normal physiological functions of cardiovascular systems of skeletal fluorosis patients are still subject to a certain level of interference after 18 years of water quality improvement and fluoride reduction. The abnormal ECG changes can be significantly recovered in mild and moderate skeletal fluorosis patients, but in severe skeletal fluorosis patients, the recovery speed may be very slow. The long-term effects of severe skeletal fluorosis on the changes in patients’ ECGs remain to be further investigated.
Objective To study the patients with acute pancreatitis blood line infection patients with intestinal barrier clinical correlation research and observation for severe acute pancreatitis blood line infection and the relationship between postoperative infection.Methods The data and cases accepted treatment for severe acute pancreatitis and diagnosed with blood line infected patients clinical treatment group,control group with the conventional treatment,the treatment group in the control group treatment on the basis of implementation of intestinal barrier early protection measures.Compared two groups gastrointestinal function score and APACHE Ⅱ score,C-reactive protein(CRP),serum tumor necrosis factor alpha(TNF alpha),plasma endotoxin level(LPS),urinary lactulose/mannitol value(L/M),and the two groups after treatment in patients with general condition comparison.Results The observation group after treatment,APACHE Ⅱ score,CRP,TNF alpha,LPS than before treatment down significantly,compared with controls differences also had statistical significance(P 0.01).Conclusion Early intestinal barrier function can effectively improve the protection of patients with gastrointestinal function,reduce the intestinal barrier permeability and inflammatory reaction.
Therapy discontinuation of systemic glucocorticoid treatment for pemphigus remains uncertain at the clinical end point of complete remission. The objective of this study was to identify the factors associated with achieving complete remission off therapy (CROT) and analyze the occurrence of relapse after therapy discontinuation. A retrospective cohort study was conducted at the Department of Dermatology of Peking University First Hospital. A total of 447 patients with pemphigus treated from 2005 to 2020 were identified. Univariate and multivariate analyses were conducted to analyze the associated factors of CROT and to evaluate the outcomes. The mean age was 48 years (±13.4 years), and 54.6% of the patients were women. During a median follow-up of 59 months (43-87.5 months), 160 of 447 (35.8%) patients achieved CROT after a median treatment duration of 51 months (38-66.2 months). Patients with a shorter therapy duration to complete remission on minimal therapy and negative desmoglein antibodies tested in remission were more likely to achieve early CROT. Thirty-five of 160 (21.9%) patients experienced relapse after CROT. Patients who discontinued therapy without guidance experienced significantly faster and higher occurrences of relapse than those withdrawing under guidance (log-rank p = 0.01). Minimal therapy maintenance ≤8 months from complete remission on minimal therapy and positive desmoglein antibodies tested at withdrawal increased the risk of early relapse after CROT.
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