Haiying Li

National Astronomical Observatories

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Co-Authors

Haitao Shen

Soochow University

Gang Chen

Soochow University

Zhong Wang

Soochow University

Ezra Burstein

The University of Texas Southwestern Medical Center

William H. Peranteau

Children's Hospital of Philadelphia

Xiang Li

First Affiliated Hospital of Soochow University

Alan W. Flake

Children's Hospital of Philadelphia

Renying Zhuo

Chinese Academy of Forestry

Yingping Wu

Xinjiang Agricultural University

Petro Starokadomskyy

The University of Texas Southwestern Medical Center

Cooperative Institutions

Chinese Academy of Sciences

171

Soochow University

168

First Affiliated Hospital of Soochow University

127

University of Chinese Academy of Sciences

Sun Yat-sen University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sichuan University

Shanghai Jiao Tong University

Peking University

Capital Medical University

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Expressed salt stress induced full length-cDNA library of Salix matsudana using yeast Invsc1.

Zhejiang Linxueyuan xuebao (2009)

Yang Ye Jing Jiang Qiao GuiRong Jing Zhou Yin Chen

The studies of Salix matsudana on salt-resistance molecular mechanism and searching new salttolerant relation genes are limited at present. It is necessary to develop drought-tolerant gene using this valuable tree resource,so a salt stress induced full-length cDNA Library of Salix matsudana was constructed using Clontech Laboratories,Inc. SMART cDNA Library Construction Protocol. Total RNA was extracted from S. matsudana under 100 mmol·L-1 NaCl stress for 24 h. After synthesizing the first-strand cDNA,the double-strand cDNA was amplified with the long-distance Polymerase Chain Reaction (LD-PCR). The result showed that insertion fragment was about 1 000 bp length in the library by PCR. The full-length cDNA fragment was linked to yeast vector pYES2.1 by pYES2.1 TOPO TA Expression Kit; then the linked product was transferred into yeast Invsc1. Finally,clones were selected by SC-U medium containing 80-130 g·L-1 NaCl. The experiment shows that the salt-tolerance of transformants yeast improved greatly compared with the wild-type yeast. The results provide an important tool for the study of cloning new salt-tolerance relation genes and the salt tolerance mechanisms of the S. matsudana. [Ch,6 fig. 17 ref.]

Cloning (programming)

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Citations (1)

Effects of arsenic trioxide combined with platinum drugs in treatment of cervical cancer: A protocol for systematic review and meta-analysis of randomized controlled trials

YaWen Zhang Di Pan HaiShi Yang Zige Li JiaXin Huang

Arsenic Trioxide

10.37766/inplasy2020.8.0130

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Citations (1)

Comparative population genomics analysis uncovers genomic footprints and genes influencing body weight trait in Chinese indigenous chicken

Poultry Science (2023)

Huie Wang Xiurong Zhao Junhui Wen Chengqian Wang Xinye Zhang

Body weight of chicken is a typical quantitative trait, which shows phenotypic variations due to selective breeding. Despite some QTL loci have been obtained, the body weight of native chicken breeds in different geographic regions varies greatly, its genetic basis remains unresolved questions. To address this issue, we analyzed 117 Chinese indigenous chickens from 10 breeds (Huiyang Bearded, Xinhua, Hotan Black, Baicheng You, Liyang, Yunyang Da, Jining Bairi, Lindian, Beijing You, Tibetan). We applied fixation index (FST) analysis to find selected genomic regions and genes associated with body weight traits. Our study suggests that NELL1, XYLT1, and NCAPG/LCORL genes are strongly selected in the body weight trait of Chinese indigenous chicken breeds. In addition, the IL1RAPL1 gene was strongly selected in large body weight chickens, while the PCDH17 and CADM2 genes were strongly selected in small body weight chickens. This result suggests that the patterns of genetic variation of native chicken and commercial chicken, and/or distinct local chicken breeds may follow different evolutionary mechanisms.

Trait

Phenotypic trait

Genetic Analysis

10.1016/j.psj.2023.103031

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Citations (2)

ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae

Molecular Microbiology (2003)

Ping Luo Haiying Li Donald A. Morrison

Summary Natural competence for genetic transformation in Streptococcus pneumoniae is achieved directly by specific proteins that are involved in DNA uptake and chromosomal recombination, and is regulated indirectly by a quorum‐sensing system encoded by two loci, comAB and comCDE . The alternative sigma factor, ComX, is thought to be the unique link between quorum sensing and competence‐specific genes. To test this hypothesis, we replaced the quorum‐sensing inducible promoter (P Q ) of the comX gene with either a constitutive promoter (P C ) or a raffinose‐inducible promoter (P R ), so that comX transcription would be independent of quorum sensing. Surprisingly, both competence and expression of late genes, such as ssbB , cglA or celB , were found to depend on CSP in these mutants. An unknown, CSP‐dependent regulator was needed when comX was expressed from these ectopic promoters, and it appears to act post‐transcriptionally. However, when a multicopy nisin‐inducible ComX‐overexpressing plasmid was introduced, pneumococcal cells developed competence in the presence of nisin even despite deletion of comE . At 1% of the normal protein peak level, ComX protein stimulated competence without the participation of the pheromone response circuit. Thus, ComX is a unique link to competence‐specific genes, but depends on multiple outputs of quorum sensing for maximal expression.

10.1046/j.1365-2958.2003.03714.x

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Citations (106)

Over-expression of the Arabidopsis Na+/H+ antiporter gene in Populus deltoides CL × P. euramericana CL “NL895” enhances its salt tolerance

Acta Physiologiae Plantarum (2010)

Guirong Qiao Renying Zhuo Mingying Liu Jing Jiang Haiying Li

Salt lake

10.1007/s11738-010-0591-x

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Citations (9)

Protection against sepsis-induced lung injury by selective inhibition of protein kinase C-δ (δ-PKC)

Journal of Leukocyte Biology (2010)

Laurie E. Kilpatrick Stephen W. Standage Haiying Li Nichelle Raj Helen M. Korchak

Inflammation and proinflammatory mediators are activators of δ-PKC. In vitro, δ-PKC regulates proinflammatory signaling in neutrophils and endothelial and epithelial cells, cells that can contribute to lung tissue damage associated with inflammation. In this study, a specific δ-PKC TAT peptide inhibitor was used to test the hypothesis that inhibition of δ-PKC would attenuate lung injury in an animal model of ARDS. Experimental ARDS was induced in rats via 2CLP, a model of polymicrobial sepsis. Following 2CLP surgery, the δ-PKC TAT inhibitory peptide (2CLP+δ-PKC TAT in PBS) or PBS (2CLP+PBS) was administered intratracheally. Controls consisted of SO, where animals underwent a laparotomy without 2CLP. Twenty-four hours after SO or 2CLP, blood, BALF, and lung tissue were collected. 2CLP induced δ-PKC phosphorylation in the lung within 24 h. Treatment with the δ-PKC TAT inhibitory peptide significantly decreased pulmonary δ-PKC phosphorylation, indicating effective inhibition of δ-PKC activation. Plasma and BALF levels of the chemokines CINC-1 and MIP-2 were elevated in 2CLP + PBS rats as compared with SO rats. Treatment with δ-PKC TAT reduced 2CLP-induced elevations in chemokine levels in BALF and plasma, suggesting that δ-PKC modulated chemokine expression. Most importantly, intratracheal administration of δ-PKC TAT peptide significantly attenuated inflammatory cell infiltration, disruption of lung architecture, and pulmonary edema associated with 2CLP. Thus, δ-PKC is an important regulator of proinflammatory events in the lung. Targeted inhibition of δ-PKC exerted a lung-protective effect 24 h after 2CLP.

Proinflammatory cytokine

10.1189/jlb.0510281

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Citations (51)

COMMD1 regulates inflammation and colitis-associated cancer progression

OncoImmunology (2014)

Haiying Li Ezra Burstein

NF-κB is a master transcriptional regulator of inflammation that plays an important role in oncogenesis, particularly in tumors that arise in the context of inflammation. Copper metabolism MURR1 domain-containing 1 (COMMD1) is a negative regulator of NF-κB. Recent genetic-based studies in both mice and human patients indicate that COMMD1 has an important role in controlling intestinal inflammation and constraining progression to colitis-associated cancer.

10.4161/21624011.2014.947891

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Citations (9)

Exosomes from C2C12 myoblasts enhance osteogenic differentiation of MC3T3-E1 pre-osteoblasts by delivering miR-27a-3p

Biochemical and Biophysical Research Communications (2018)

Qian Xu Yazhou Cui Jing Luan Xiaoyan Zhou Haiying Li

C2C12

Myokine

Exosome

10.1016/j.bbrc.2018.02.144

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Citations (89)