Objective: To investigate the HPCE fingerprint of Dipsaci Radix.Methods: The HPCE fingerprint was established using a 50 μm× 65 cm uncoated fused silica capillary(effective length 57 cm),a buffer comprising 50 mmol·L-1 borate-boric acid at pH 9.20,a running voltage of 25 kV,a capillary temperature of 25 ℃ and a wavelength of 212 nm.The sample was injected under pressure of 5 kPa for 30 s.The HPCE fingerprint was evaluated by the software Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine Version 2004A and clustering analysis.Results: By analyzing the HPCE fingerprint,high similarities were found among the 12 samples.The similarity coefficients of the two batches of samples from Hubei were from 0.8 to 0.9,and those of the remaining samples from Sichuan were greater than 0.9.Fourteen major co-possessing peaks were selected as the characteristic fingerprint peaks of Dipsaci Radix.Clustering results were consistent with the similarity analysis.Conclusion: The developed method is accurate and reliable,and the fingerprint analysis can thus be used for the quality assessment and control of Dipsaci Radix.
Aiming at the medical practice problems of the surgical steel medical instruments, such as the crevice corrosion, the poor mechanical compatibility and the Ni, Cr plasma exudation, the laser deposition of Ti-6Al-4V alloy cladding layer at the local functional area as alternative coating was proposed and realized as a new process method. The accurate element content and good formability Ti-6Al-4V cladding powder was chosen, the low power and high duty cycle optimized laser process was adopt, the alternative coating of good fusion and low dilution was prepared. Through the elemental line scanning, the interface microstructure analysis and the experiments of basic mechanical properties, the basic properties of the cladding were characterized and verified. The experiments results showed that, the Ti, Al and V contents of the top coating were respectively about 88%, 4.9% and 3.9%, no sensitizing ions such as Cr and Ni were detected. Initial equiaxed α phase, flake β phase dist were distributed in the coating and interface, the α' martensite was precipitated at the boundary of the flake β phase, some refined granular β phase dispersion pinned to the grain boundary of basket structure. The microhardness of cladding layer was 352.08~312.76 HV0.1. The friction coefficient of the cladding layer was about 0.22~0.65. A new technology and method reference for improving and upgrading the performance of surgical medical devices is provided by this research.
Th2 immune cells infiltration into nasal mucosa is one of the characters of allergic rhinitis (AR). We aimed to explore whether inhibition of Th2 immune cells infiltration would attenuate AR progression. AR mouse model was established by i.p. injection of ovalbumin (OVA). The infiltrated immune cells into nasal lavage fluid were detected by flow cytometry. Cytokine concentration in serum was determined by ELISA. AR mice symptoms were indicated by the number of sneezing and nasal rubbing events. In AR mice, CCL2 expression levels and CD45+CD11b+Ly6Chi inflammatory monocytes cells significantly increased as compared with control mice. CCL2 siRNA encapsulated nanoparticles (NPsiCCL2) prevent CCL2 expression and inflammatory monocytes infiltration in AR mice. NPsiCCL2 treatment dramatically decreased the number of sneezing and nasal rubbing events in AR mice. Moreover, NPsiCCL2 treatment attenuated serum OVA-specific IgE, OVA-specific IgG1 and histamine levels. Mechanically, NPsiCCL2 treatment attenuates AR symptoms via inhibiting Th2 cytokine (IL-4, IL-5 and IL-13) production. Nanomedicine-mediated prevention of inflammatory monocytes infiltration ameliorates ovalbumin-induced allergic rhinitis in mouse model.
Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.
Objective To observe whether doxapram can attenuate the degree of somnolence arising from butorphanol intravenous analgesia. Methods One hundred and five adult patients were randomly divided into three groups receiving continuous intravenous analgesia with 0.01% butorphanol and 0.1% doxapram analgesia-pump (group Ⅰ , n=35), 0.01% butorphanol and 0.15% doxapram analgesia-pump ( group Ⅱ , n=35 ), and 0.01% butorphanol analgesia-pump ( group Ⅲ, n = 35 ) after epidural block. The analgesia effect and lethargic side-effect were compared . Results OAA/S (at 8 h-24 h after operation ) in group Ⅲ was larger than those in group Ⅰ and Ⅱ (P 0.05, VAS ≤3 respectively). Side-effects between three groups were not significantly different. Conclusion Doxa-pram is effective for attenuating lethargy symptom during intravenous butorphanol analgesia with less side-effects.
Key words:
doxapram; butorphanol; combination; analgesia; side-effect
Objective To study the effect of fentanyl with different injection technigues on fentanyl-induced cough.Methods One hundred and seventy-five patients undergoing general anesthesia were randomly allocated into five groups with 35 cases each.The concentration of fentanyl and injection time were 50 μg/ml and 3-5 s in group Ⅰ,20 μg/ml and 3-5 s in group Ⅱ,50 μg/ml and ≥30 s in group Ⅲ,20 μg/ml and ≥30 s in group Ⅳ.Propofol 1-1.5 mg/kg was given at 1 min before fentanyl 50 μg/ml was administered as that in group Ⅰ.The dose of fentanyl was 3 μg/kg in all patients.To observe the state of cough after injection.Results Fentanyl-induced coughing was significantly less in groups Ⅳ and Ⅴ(11.4% and 3.0%) than that in groupⅠ(34.3%)(P0.05 or P0.01).The severity of coughing was significantly less in group Ⅴthan that in group Ⅰ(P0.05).Conclusion Fentanyl-induced cough can be reduced by diluted solution and slow injection,and better by pre-injection of propofol.
Aim To evaluate the clinical effect of the nitinol (NiTi)-patellar concentrator (NT-PC) for the treatment of comminuted patellar fractures. Material and methods A total of 32 patients with acute comminuted patellar fracture accepted open reduction and internal fixation with the NT-PC, and the curative effects were evaluated using the Böstman clinical grading scale. Results All fractures were anatomically reduced by surgery and all cases were followed-up for six to 18 months. The mean score of patients according to the Böstman clinical grading scale was 25.6, with 29 of 32 (90.7%) patients achieving excellent or good results. Two patients had traumatic arthritis, one had slippage of the NT-PC, and all patients received pharmacotherapy. Conclusions The application of the NT-PC is a satisfactory approach to the treatment of comminuted patellar fractures.
BACKGROUND We report a rare case of cervical spinal canal penetrating trauma and review the relevant literatures. CASE SUMMARY A 58-year-old male patient was admitted to the emergency department with a steel bar penetrating the neck, without signs of neurological deficit. Computed tomography (CT) demonstrated that the steel bar had penetrated the cervical spinal canal at the C6–7 level, causing C6 and C7 vertebral body fracture, C6 left lamina fracture, left facet joint fracture, and penetration of the cervical spinal cord. The steel bar was successfully removed through an open surgical procedure by a multidisciplinary team. During the surgery, we found that the cervical vertebra, cervical spinal canal and cervical spinal cord were all severely injured. Postoperative CT demonstrated severe penetration of the cervical spinal canal but the patient returned to a fully functional level without any neurological deficits. CONCLUSION Even with a serious cervical spinal canal penetrating trauma, the patient could resume normal work and life after appropriate treatment.
To compare perioperative hidden blood loss in the treatment of femoral intertrochanteric fractures with percutaneous compression plate (PCCP) and proximal femoral nail anti-rotation (PFNA) in elderly patients, and analyse its influencing factors in order to provide the necessary data support for clinical perioperative treatment, and choice of appropriate internal fixation method.Retrospective analyses was carried out on data obtained from 158 patients with intertrochanteric fracture treated with PCCP or PFNA from January 2010 to May 2017.Data were obtained from variables such as age, gender, height, weight, operative bleeding and postoperative drainage, operation time, etc. Upon blood routine examination before and after surgery (RBC, Hb and Hct), total and hidden blood losses were calculated using Gross equations.A comparative analysis was carried out on the differences in hidden blood loss, postoperative complications and prognosis between PCCP and PFNA.Visible blood loss was higher in PCCP than in PFNA, but total and hidden blood losses were significantly lower in PFNA(P<0.05).Within the two groups, hidden blood loss in elderly patients was significantly higher (P<0.05).Start time of weight-bearing of PCCP group was significantly longer than of PFNA group (P<0.05),but incidences of complications of important organs and perioperative mortality in PCCP group were significantly lower than those of PFNA group (P<0.05).There were no significant differences in operation time, infection of incision, failure of internal fixation, fracture healing time, or Harris function score of half year after operation between the two groups (P<0.05).There is serious hidden blood loss before and after operation in elderly patients with femoral intertrochanteric fractures, of which intramedullary fixation is higher.Intramedullary fixation causes more severe complications of important organs than extramedullary fixation.
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