Man Xue

Jinzhou Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jing-Shan Lu

Xi'an Jiaotong University

Xiaodong Ding

Northeast Agricultural University

Qi‐Yu Chen

Tea Research Institute

Xu‐Hui Li

Xi'an Jiaotong University

Minglong Li

Northeast Agricultural University

Min Zhuo

University of Toronto

Qiang Li

Northeast Agricultural University

Jialei Xiao

Northeast Agricultural University

Xingchen Cao

East China University of Science and Technology

Shuzhen Zhang

Army Medical University

Cooperative Institutions

Shanghai Jiao Tong University

Chinese Academy of Sciences

Capital Medical University

Xi'an Jiaotong University

Chinese Academy of Medical Sciences & Peking Union Medical College

Beijing Tian Tan Hospital

Northeast Agricultural University

Fudan University

Shandong Agricultural University

Soochow University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Content determination of hesperidin in Wuwei granules by HPLC

Central South Pharmacy (2013)

Man Xue

Objective To establish a quality control method for hesperidin in Wuwei granules by RP-HPLC.Methods A Kromasil C18 column(150 mm×4.6 mm,5 μm) was used at 30 ℃;the mobile phase was acetonitrile-water(20 :80).The detection wavelength was 284 nm and the flow rate was 1.0 mL·min-1.Results Within the range of 19.96-159.68 μg·mL-1,hesperidin showed a good linear relationship between the injection amount and peak areas(r= 0.999 8).The average recovery was 99.0% and RSD was 1.3%(n=9).Conclusion The method is accurate and reliable,and it can be used for the quality control of Wuwei granules.

Linear range

Linear relationship

Source

Cite

Citations (0)

Determination of Baicalin in Jinchan Oral Liquid by TLC Scanning

Anti-infection Pharmacy (2008)

Man Xue

Objective:To establish a method for quality control of Jinchan oral liquid,the content of Baicalin in Jinchan oral liquid was determined by thin layer chromatographic scanning(TLCS).Methods:Baicalin of Jinchan oral liquid was separated byφ(C_2H_4O_2,HAC)=36% acids in polyamide film;the method of the dual wavelength TLCS was adopted to determine the content of baicalin in Jinchan oral liquid and the detection wave-length wasλ_2=278 nm,λ_R= 370 nm.Results:The content of baicalin between 0.217～1.083μg/mL showed a good linearity.The equation of linear regression was A=2 131.551+7.545 C,r=0.999 3.The average recovery was 101.60% and RSD was 2.50%(n=3). Conclusion:This method is accurate and simple.It can be used to determine baicalin in Jinchan oral liquid.

Baicalin

Content determination

Linear relationship

Thin layers

Source

Cite

Citations (0)

Quality Control on Compound Danggui Qizao Jing Oral Solution

Anti-infection Pharmacy (2012)

Man Xue

Objective:To establish the quality control on compound for Danggui Qizao Jing oral solution. Methods: Angelicae Sinensis Radix in Danggui Qizao Jing oral solution was identified by TLC. The content of ferulic acid was determined by HPLC. Results: The spots in the TLC were fairly clear, and no interference was shown in the blank samples. Ferulic acid showed a good linear relationship within a range of 0.06 and 1.2μg/mL (r=0.9998). The average rate of recovery was 96.89% and the RSD was 0.76%. The average mass concentration of ferulic acid was 11.83 ug/mL.Conclusion: The method is simple, accurate and reproducible. It could be used for the quality control of Danggui Qizao Jing oral solution.

Angelica sinensis

Radix (gastropod)

Content determination

Source

Cite

Citations (0)

Exploration of n-6 and n-3 Polyunsaturated Fatty Acids Metabolites Associated with Nutritional Levels in Patients with Severe Stable Chronic Obstructive Pulmonary Disease

DOAJ (DOAJ: Directory of Open Access Journals) (2020)

Man Xue Cai Chen L Guan Yongsheng Xu Jing Lin

Source

Cite

Citations (0)

Downregulated ZNF132 Predicts Unfavorable Outcomes in Breast Cancer via Hypermethylation Modification

Research Square (Research Square) (2019)

Zhao Liu Jiaxin Liu Man Xue Weifan Zhang Xinhui Zhao

Abstract Background: Methylation modification of tumor suppressor gene is one of the most critical mechanisms in human breast cancer (BC). Some biomarkers, including HER2, ER and PR, have been used in clinical practice, however, the effective was limited in terms of high recurrence and mortality rate. Thus, a better diagnostic and therapeutic target for BC is urgent. To date, no study provide information on the status of ZNF132 in BC or analyze diagnosis and prognostic significance of ZNF132 in BC. Methods: In the present study, we investigated the expression and clinical signifcance of ZNF132 by TCGA database and clinical samples analysis. Besides, a further exploration was performed to assess the molecular mechanisms of ZNF132 using the multiple bioinformatic tools. Importantly, a MSP assay was executed to confirm the epigenetic alteration of ZNF132. Results: Our results showed that the mRNA expression of ZNF132 was significantly downregulated in BC tissues, the consistent results was obtained by immunohistochemistry assessment. Importantly, survial analysis revealed that the lower expression of ZNF132 was remarkably correlated to Relapse Free Survival (RFS), but not Overall Survival (OS). Besides, the ROC curve confirmed ZNF132 had powerful sensitivity and specificity to distinguish between BC and adjacent normal tissues. Finally, MSP analysis demonstrated that ZNF132 was hypermethylated in BC tissues. Interestingly, ZNF132 methylation level was negatively correlated with its gene expression. Conclusions: Our results revealed that hypermethylation of ZNF132 contributed to its downregulated expression and could be identified as a new diagnostic and prognostic marker in BC.

Clinical Significance

10.21203/rs.2.15590/v1

Cite

Citations (0)

[Study on biocompatibility of skin reproductive membrane].

PubMed (2000)

Liqun Ning Man Xue Huang Hn

To study the biocompatibility of skin reproductive membrane.According to ISO's standards, the extractions of the skin reproductive membrane were prepared, and the acute systematic toxicity test, primary skin irritant test, cytotoxicity test, gene expression of type I collagen and fibronectin were detected to evaluate the biocompatibility of skin reproductive membrane.All of those tests showed negative results.The skin reproductive membrane has excellent biocompatibility in the level of the systematic, cellular and molecular biology.

Biocompatibility

Reproductive toxicity

Source

Cite

Citations (2)

The Phosphorylation of Wild Soybean (Glycine soja) Splicing Factor GsSCL30a by GsSnRK1 Regulates the Tolerance of Soybean to Alkali Stress

Authorea (Authorea) (2024)

Minglong Li X. Liu Xin‐Huai Zhao Fujing Liu Man Xue

The splicing of pre-mRNA can be constitutive or alternative. The resulting transcripts may play significant roles in plant development and responses to environmental changes. Wild soybean, as a wild relative of cultivated soybean, has excellent traits such as alkali tolerance and is an ideal material for studying stress mechanisms and mining resistant genes. In this study, we identified a splicing factor GsSCL30a of the SR family that depends on the GsSnRK1 protein kinase. Y2H and BiFC assays verified the interaction between GsSnRK1 and GsSCL30a. In vitro phosphorylation experiments confirmed that GsSnRK1 could phosphorylate GsSCL30a, and six phosphorylation sites of GsSCL30a by GsSnRK1 were determined. qRT-PCR results showed that the expression level of GsSCL30a was highest in wild soybean leaves, and its transcription level was upregulated under alkali stress. Splicing factor reporter analysis found that GsSCL30a could self-splice the third intron, which contains multiple conserved regions of the GAAG motif. In addition, the phosphorylation of GsSCL30a by GsSnRK1 promoted its self-splicing. Y2H experiments proved that there is a physical interaction between GsSCL30a and the U1 snRNP-specific protein U1-70K, indicating that GsSCL30a plays an important role at the 5' splicing site. We overexpressed GsSCL30a-GsSnRK1 in soybean hairy roots. It was found that OE# GsSCL30a-GsSnRK1 could synergistically enhance the tolerance of soybean to alkali stress. Our study revealed the response mechanism of GsSCL30a in soybean under alkali stress conditions, that is, GsSCL30a relies on GsSnRK1 for phosphorylation modification during the alkali stress process, which affects the activity of the splicing factor and then regulates the plant's response to alkali stress, providing a new idea for improving the stress resistance ability of crops.

Glycine soja

10.22541/au.173269101.17797756/v1

Cite

Citations (0)

Inhibiting Neuronal Adenylyl Cyclase Subtype 1 (AC1) for Treating Anxiety and Headache in the Animal Model of Migraine

SSRN Electronic Journal (2022)

Ren-Hao Liu Mingjie Zhang Man Xue Tao Wang Jing-Shan Lu

Migraines are a common medical condition, currently without effective treatment. The recently developed CGRP treatment is expensive and with limited beneficial effect. From a basic science point of view, the central mechanism for migraine and headache is largely unknown. In the present study, we demonstrate that cortical excitatory transmission is significantly enhanced in the ACC - a brain region which is critical for pain perception. Biochemical studies found that both of NMDA receptor GluN2B serine 1303 phosphorylation and AMPA receptor GluA1 serine 831 and serine 845 phosphorylation levels were enhanced in ACC of migraine rats. Both the presynaptic release of glutamate, and postsynaptic responses of AMPA receptors and NMDA receptors were enhanced. Furthermore, behavioral anxiety and nociceptive responses, were increased. Synaptic long-term potentiation (LTP) is occluded since theta-burst stimulation (TBS) failed to induce LTP in migraine rats. Inhibition of cortical excitation by application of a selective AC1 chemical inhibitor NB001 within the ACC significantly reduced behavioral sensitization and anxiety. Our results provide strong evidence that cortical LTPs contribute to migraine-related pain and anxiety, and drugs that inhibit cortical excitation such as NB001 may serves as potential new medicines for treating migraine in the future.

10.2139/ssrn.4309079

Cite

Citations (0)

The Synchronized Progression from Mitosis to Meiosis in Female Primordial Germ Cells between Layers and Broilers

Genes (2023)

Yuxiao Ma Wenhui Wu Yun Zhang Xuzhao Wang Jiahui Wei

Layer and broiler hens show a dramatic difference in the volume and frequency of egg production. However, it is unclear whether the intrinsic competency of oocyte generation is also different between the two types of chicken. All oocytes were derived from the primordial germ cells (PGC) in the developing embryo, and female PGC proliferation (mitosis) and the subsequent differentiation (meiosis) determine the ultimate ovarian pool of germ cells available for future ovulation. In this study, we systematically compared the cellular phenotype and gene expression patterns during PGC mitosis (embryonic day 10, E10) and meiosis (E14) between female layers and broilers to determine whether the early germ cell development is also subjected to the selective breeding of egg production traits. We found that PGCs from E10 showed much higher activity in cell propagation and were enriched in cell proliferation signaling pathways than PGCs from E14 in both types of chicken. A common set of genes, namely insulin-like growth factor 2 (IGF2) and E2F transcription factor 4 (E2F4), were identified as the major regulators of cell proliferation in E10 PGCs of both strains. In addition, we found that E14 PGCs from both strains showed an equal ability to initiate meiosis, which was associated with the upregulation of key genes for meiotic initiation. The intrinsic cellular dynamics during the transition from proliferation to differentiation of female germ cells were conserved between layers and broilers. Hence, we surmise that other non-cell autonomous mechanisms involved in germ-somatic cell interactions would contribute to the divergence of egg production performance between layers and broilers.

10.3390/genes14040781

Cite

Citations (1)

Current Progress and Outlook for Agrimonolide: A Promising Bioactive Compound from Agrimonia pilosa Ledeb.

Pharmaceuticals (2023)

Ting Huang Chun‐Cao Zhao Man Xue Yun‐Feng Cao Liang-Kang Chen

Agrimonolide (AM), which is a derivative of isocoumarins, is found mainly in the herb Agrimonia pilosa Ledeb. This compound is highly lipophilic and readily crosses the blood–brain barrier. In recent years, interest has grown in the use of AM as a multitarget natural treatment for various diseases, such as cancer, inflammation, hepatic injury, myocardial damage, and diabetes mellitus. The potential mechanisms of these pharmacological effects have been clarified at cellular and molecular levels. AM shows no cytotoxicity over a range of concentrations in different types of cells, providing evidence for its good safety profile in vitro. These findings indicate that AM is a promising medicinal agent. However, most studies on AM’s pharmacological activities, mechanisms of action, and safety lack substantial animal or human data. Additionally, the pharmacokinetics, metabolism, and disposition of this compound have received little attention. This review highlights the status of current information regarding the sources, properties, pharmacological effects, and safety of AM. Furthermore, potential strategies to resolve problematic issues identified in previous studies are fully discussed. This summary and analysis of the research progress of AM may inspire deeper investigations and more extensive applications of AM in the future.

Natural compound

Safety profile

10.3390/ph16020150

Cite

Citations (2)