Maria Olívia Pereira

University of Minho

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Ana Margarida Sousa

University of Minho

M. J. Vieira

University of Minho

Susana Patrícia Lopes

University of Minho

Idalina Machado

University of Minho

Anália Lourenço

University of Minho

Manuel Simões

Universidade do Porto

Nuno F. Azevedo

Universidade do Porto

Diana Alves

University of Minho

Paula Jorge

University of Minho

Andreia Patrícia Magalhães

International Iberian Nanotechnology Laboratory

Cooperative Institutions

University of Minho

Edith Cowan University

University of Wisconsin–Stout

Institute for Biotechnology and Bioengineering

Universidade do Porto

University of Lisbon

Universidade Federal do Ceará

Universidade Estadual do Ceará

University of Coimbra

Universidade Nova de Lisboa

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Bridging tourism theory and practice (2024)

Jafar Jafari Noel Scott Manuela Guerreiro Patrícia Pinto Destination Nunzia Borrelli

Recognizing the increasing gap between what is researched in academic communityand what is practiced in industry, this series aims to bring together academic and industry leaders in their respective fields to discuss, exchange, and debate issues critical to the advancement of tourism.The book series intends to not only create a platform for academics and practitioners to share theories and practices with each other, but more importantly to serve as a collaborative venue for meaningful synthesis.

10.1108/s2042-144320240000014017

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Effect of flow regime on the architecture of a Pseudomonas fluorescens biofilm

Biotechnology and Bioengineering (2002)

Maria Olívia Pereira M. Kuehn Stefan Wuertz Thomas R. Neu L. F. Melo

Abstract A comparison of the effects of laminar versus turbulent flow regime on the characteristics of a single‐species biofilm is presented. The study was carried out by growing Pseudomonas fluorescens biofilms in a flow cell and studying the different layers of the biological matrix with a confocal laser‐scanning microscope. The following conclusions were obtained: i) a higher concentration of cells was found in the upper layers of the microbial films than in their inner layers, regardless of the flow regime; ii) the fraction of cells in the overall biofilm mass decreased with time as the film grew; and iii) under laminar flow the total number of cells was higher than in biofilms formed under turbulent flow, but the latter had a higher number of cells per unit volume. Such conclusions, together with the fact that the biofilms were more dense and stable when formed in contact with turbulent flows, favor the design of more compact and efficient biofilm reactors operating in turbulent conditions. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 164–171, 2002; DOI 10.1002/bit.10189

Pseudomonas fluorescens

Laminar sublayer

Flow conditions

10.1002/bit.10189

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Citations (205)

An harmonised vocabulary for communicating and interchanging biofilms experimental results.

PubMed (2014)

Ana Margarida Sousa Maria Olívia Pereira Nuno F. Azevedo Anália Lourenço

Biofilm studies are at the crossroads of Biology, Chemistry, Medicine, Material Science and Engineering, among other fields. Data harmonisation in Biofilms is therefore crucial to allow for researchers to collaborate, interchange, understand, and replicate studies at an inter-laboratory and inter-domain scale. The international Minimum Information About a Biofilms Experiment initiative has prepared a set of guidelines for documenting biofilms experiments and data, namely the minimum information checklist. This paper goes a step forward and describes a new ontology for the broad description of biofilm experiments and data. In such an interdisciplinary context we chose to rely on a common integration framework provided by a foundational ontology that facilitates the addition and extension of various sub-domain modules, and the consistent integration of terminology extracted from several existing ontologies, e.g. EXPO and ChEBI. The community is participating actively in the production of this resource, and it is already used by public biofilms-centred databases, such as BiofOmics, and bioinformatics tools, such as the Biofilms Experiment Workbench. This practical validation serves the purpose of disseminating the controlled vocabulary among researchers and identifying current limitations, glitches, and inconsistencies. Information branches will be added, extended or refactored according to user feedback and group discussions.

Controlled vocabulary

Workbench

10.2390/biecoll-jib-2014-249

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Role of planktonic and sessile extracellular signals on interspecies relationships

Susana Patrícia Lopes Idalina Machado M. J. Vieira Maria Olívia Pereira

Universidade do Minho. Centro de Engenharia Biologica. Instituto de Biotecnologia e Bioengenharia (IBB-CEB) e Fundacao para a Ciencia e a Tecnologia (FCT) - PTDC/SAU-ESA/64609/2006; SFRH/BD/31065/2006

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Exploring new treatment strategies for Pseudomonas aeruginosa biofilm infections based on plant essential oils

Formatex Research Center eBooks (2013)

Filipa Alexandra Baltar Lobo Coelho Maria Olívia Pereira

Pseudomonas aeruginosa is an important cause of nosocomial and biofilm-associated infections which is often difficult to eradicate, due to its intrinsic resistance and capacity to undergo mutations and adaptations. This study aimed to assess the anti-biofilm activity of three essential oils (EOs) (Cinammon (Cinnamomum zeylanicum), Tea tree (TTO) (Melaleuca alternifolia) and Palmarosa (Cymbopogon martini), and the existence of synergistic effects of TTO and its major component (Terpinen-4-ol (T4ol)) combined with Ciprofloxacin (CIP), against 24-hour-old P. aeruginosa biofilms. Results showed that the treatment of pre-established P. aeruginosa biofilms with the three plant EOs, T4ol and CIP, individually, promoted a significant decrease of biofilm biomass and the number of biofilm entrapped cells, mainly for higher concentrations. Nevertheless, the antimicrobial synergisms combining TTO or T4ol with CIP revealed to be more successful, since significant disturbance of biofilms occurs for lower concentrations of the products. T4ol associated with CIP was the combination that revealed the most promising synergistic effects. Therefore, T4ol-CIP association may be a useful therapeutic option to treat P. aeruginosa biofilm infections.

Melaleuca alternifolia

Cinnamomum zeylanicum

Biocide

Melaleuca

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Citations (11)

Role of the polymeric matrix constituents on the performance of a biocide against pseudomonas fluorescens biofilms

Maria Olívia Pereira M. J. Vieira

Biocide

Pseudomonas fluorescens

Matrix (chemical analysis)

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Gestão da Qualidade na Administração Pública. A utilização da ferramenta CAF nos municípios portugueses

Ana Sofia Silveira Maria Olívia Pereira

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Emergent Bacteria in Cystic Fibrosis:In VitroBiofilm Formation and Resilience under Variable Oxygen Conditions

BioMed Research International (2014)

Susana Patrícia Lopes Nuno F. Azevedo Maria Olívia Pereira

Concurrent to conventional bacterial pathogens, unusual microbes are emerging from cystic fibrosis (CF) airways. Nonetheless, little is known about the contribution of these newly microbes to the resilience of CF-associated biofilms, particularly under variable-oxygen concentrations that are known to occur in vivo in the mucus of CF patients. Two CF-emergent bacterial species, Inquilinus limosus and Dolosigranulum pigrum , and the major pathogen Pseudomonas aeruginosa were studied in terms of biofilm development and antibiotic susceptibilities under in vitro atmospheres with different oxygen availabilities. All species were able to develop in vitro biofilms under different oxygen-available environments, with D. pigrum accumulating high amounts of biomass and respiratory activities. When established, biofilms were of difficult eradication, with antibiotics losing their effectiveness in comparison with the corresponding planktonic populations. Surprisingly, biofilms of each emergent organism displayed multidrug resistance under aerobic environments, enduring even in low-oxygen atmospheres. This study suggests a potential prospect on the impact of nonconventional organisms I. limosus and D. pigrum on CF lung infections, demonstrating capacity to adapt to biofilm mode of life under restricted-oxygen atmospheres resembling CF airways, which may ultimately endanger the efficacy of currently used antibiotic regimens.

Multidrug tolerance

10.1155/2014/678301

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Citations (27)

Novel nanoparticle-based therapy to eradicate Pseudomonas aeruginosa infections in cystic fibrosis lungs: dual drug release by cubosomes

Ana Margarida Sousa Maria Olívia Pereira

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Antibacterial efficacy of essential oils and its major components against planktonic and biofilm cultures of E. coli and S. aureus

F. M. Millezi Roberta Hilsdorf Píccoli J. M. Oliveira Maria Olívia Pereira

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