Abstract Rapid and ultra‐sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is critical for early screening and management of COVID‐19. Currently, the real‐time reverse transcription polymerase chain reaction (rRT‐PCR) is the primary laboratory method for diagnosing SARS‐CoV‐2. It is not suitable for at‐home COVID‐19 diagnostic test due to the long operating time, specific equipment, and professional procedures. Here an all‐printed photonic crystal (PC) microarray with portable device for at‐home COVID‐19 rapid antigen test is reported. The fluorescence‐enhanced effect of PC amplifies the fluorescence intensity of the labeled probe, achieving detection of nucleocapsid (N‐) protein down to 0.03 pg mL −1 . A portable fluorescence intensity measurement instrument gives the result (negative or positive) by the color of the indicator within 5 s after inserting the reacted PC microarray test card. The N protein in inactivated virus samples (with cycle threshold values of 26.6–40.0) can be detected. The PC microarray provides a general and easy‐to‐use method for the timely monitoring and eventual control of the global coronavirus pandemic.
CT-1 belongs to IL-6 superfamily, as a motion and sensory neurotrophic factor, which is target- derived and autocrine and/or paracrine. CT-1 can bind the receptor compounds gp130/ LIFRβ/ C-1Rα, active JAK, and induce phosphorylate of STAT. The activity of receptor compounds, JAK, STAT, MAPK and PI3-K are roles in the process, and can activate NF-кB, induce synthesize of anti-apoptosis proteins and promote phosphorylate of proteins.
Abstract Although some circular RNAs (circRNAs) were found to be translated through IRES-driven mechanism, the scope and functions of circRNA translation are unclear because endogenous IRESs are rare. To determine the prevalence and mechanism of circRNA translation, we developed a cell-based system to screen random sequences and identified 97 overrepresented hexamers that drive cap-independent circRNA translation. These IRES-like short elements are significantly enriched in endogenous circRNAs and sufficient to drive circRNA translation. We further identified multiple trans -acting factors that bind these IRES-like elements to initiate translation. Using mass-spectrometry data, hundreds of circRNA-coded peptides were identified, most of which have low abundance due to rapid degradation. As judged by mass-spectrometry, 50% of translatable endogenous circRNAs undergo rolling circle translation, several of which were experimentally validated. Consistently, mutations of the IRES-like element in one circRNA reduced its translation. Collectively, our findings suggest a pervasive translation of circRNAs, providing profound implications in translation control.
Abstract Migraine exhibits a substantial prevalence worldwide. The current diagnostic criteria rests exclusively on clinical characteristics without any objective and reliable means. The calcitonin gene‐related peptide (CGRP), as a biomarker for distinguishing migraine, undergoes swift degradation, featuring a half‐life of under 10 min, which poses a significant challenge to the point‐of‐care testing of CGRP in clinical application. Here, a photonic crystal (PC)‐based biochip has been developed to detect CGRP via the fluorescence competition assay. The chip integrates the functionalities of fluorescence enhancement and hydrophilic–hydrophobic patterning enrichment, enabling rapid and sensitive detection of CGRP. After investigating the optimal enhancement distance of fluorescence near PCs, the chip allows CGRP detection using <30 μL of saliva at room temperature within 10 min. A minimum detection limit of 0.05 pg/mL is achieved. Furthermore, CGRP concentrations in the saliva of 70 subjects have been tested by PC biochips. The results exhibit strong concordance with the enzyme‐linked immunosorbent assay (ELISA), demonstrating a linear correlation coefficient of R 2 of 0.97. This sensitive detection of markers within such a short duration surpasses the capacities of ELISA, which paves the way for establishing a precise diagnostic framework integrating clinical phenotypes and biomarkers for migraine.
Abstract Rapid detection of various exosomes is of great significance in early diagnosis and postoperative monitoring of cancers. Here, a divisional optical biochip is reported for multiplex exosome analysis via combining the self‐assembly of nanochains and precise surface patterning. Arising from resonance‐induced near‐field enhancement, the nanochains show distinct color changes after capturing target exosomes for direct visual detection. Then, a series of divisional nanochain‐based biochips conjugated with several specific antibodies are fabricated through designed hydrophilic and hydrophobic patterns. Because of the significant wettability difference, one sample droplet is precisely self‐splitting into several microdroplets enabling simultaneous identification of multiple target exosomes in 30 min with a sensitivity of 6 × 10 7 particles mL −1 , which is about two orders lower than enzyme‐linked immunosorbent assay. Apart from the trace amount detection, excellent semiquantitative capability is demonstrated to distinguish clinical exosomes from glioblastoma patients and healthy people. This method is simple, versatile, and highly efficient that can be extended as a diagnostic tool for many diseases, promoting the development of liquid biopsy.
Objective In the recent twenty years, the diaphragmatic contraction, relaxation functions and electric activity have been explored through electromyography (EMG) and transdiaphragmatic pressure (Pdi) determination But these techniques required some complex and expensive instruments, so the diagnosis and treatment of children′s diaphragmatic fatigue have not been well evaluated The present study explored the diagnosis of children′s diaphragmatic fatigue through measuring ribcage abdomen motion and analyzed its asynchrony Methods Fifty three children (male 37, female 16, and age rage from 1 months to 9 years) with respiratory rate30 breaths/min, heart rate 110 beats/min, and respiratory dysfunction had asynchronized ribcage abdomen motion showed by impedance respirograph (IRG) The authors observed whether ribcage abdomen motion was synchronic and calculated M levels (staggered peak time /total duration of the breathing cycle) The ribcage and abdomen outputs were displayed on vertical (for rib cage) and horizontal (for abdomen) axes of X Y instrument In addition, the change of respiratory frequency and heart rate was observed and arterial blood gas analysis was also performed Results (1) M levels in one dimensional IRG were positively correlated with alpha angle in two dimensional IRG ( r =0 956, P 0 001 ) Asynchronized respiratory motions could be divided into three types type Ⅰ showed completely contra directional movements of respiration, M levels for (48 1 ±4 4)%, an irregularly clockwise loop in the two dimensional IRG, and alpha angle for (138 3±15 0) degrees In type Ⅱ, one dimensional IRG showed displaced peak of the chest and abdomen motion curves, M levels were(16 5±4 7)%, two dimensional IRG was displaced in a counterclockwise direction, and alpha angle was (55 3±10 8) degrees In type Ⅲ, abdominal motion curve of one dimensional IRG had double peaks, M levels were 0, two dimensional IRG was presented as 8 shaped double circles, alpha angle was (41 3±3 8)degrees; (2) pH levels in the patients with type Ⅰand type Ⅱ diaphragmatic fatigue were significantly lower, and PCO 2 levels were significantly higher than those with type Ⅲ or in the normal subjects ( P 0 001 for all), but there was no statistically significant difference between type Ⅲ and the normal subjects ( P 0 05); (3) Both of respiratory rate and heart rate in type Ⅰ, type Ⅱ and type Ⅲ were higher than those in the normal subjects (all P 0 001), and the differences among the three types were significant ( P 0 001 for all); (4) Both M levels and alpha angle were negatively correlated with pH levels ( r =-0 514, P 0 001 and r =-0 497, P 0 001 ), while positively correlated with PCO 2 levels ( r =0 672, P 0 001 and r =0 625, P =0 01).Conclusions (1) IRG can be reliably used to diagnose children′s diaphragmatic fatigue This technique is simple and easy to perform and non invasive It is therefore worthy of recommending for further clinical investigations (2) According to the characteristics of IRG, diaphragmatic fatigue can be divided into three types (3) The development of children′s diaphragmatic fatigue has a series of characteristic changes (4) To avoid the patients suffering from respiratory failure, it is the key time to adopt the policies of prevention and treatment when IRG shows signs of type Ⅲdiaphragmatic fatigue
Objective To explore the nursing problem to prevent the complications after laparoscope-assisted radical resection for rectal carcinoma,to provide dependable nursing theories basis to paramedic by evidence-based nursing.Methods To observe 60 cases of same surgical operation average 62.5-year-old,looking for the reason in clinical nursing problems and theories basis of solving problems by evidence-based nursing.Result The research improved the knowledge of preventing the complications after the laparoscope-assisted radical resection for rectal carcinoma in nursing problems and provide theories basis.Conclusion Evidence-based theory is the base of the clinical nursing.It can solve the clinical problems by nursing research.reduce complications and improve postoperative life quality.
Fifty-seven cadaveric renal transplantations in fifty-five cases were analysed of them, thirty cases were prepared by continuous ambulatory peritoneal dialysis (CAPD group); twenty-seven transplantations in twenty-five cases were prepared by hemodialysis (HD group). Our data show that there is no significant difference in the survival duration between CAPD group and HD group; that there is also no significant difference in the survival duration between the two groups treated with the same immunosuppressive drugs, and that patients prepared by CAPD have a low risk of peritonitis after transplantation. It is better not to remove the Tenckhoff catheter until the graft function is stable so that it can be used for the transient peritoneal dialysis in the case of the insult of graft function or for the sampling of the peritoneal effusion for diagnosis of suspectable peritonitis after renal transplantation.
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