Abstract This study aimed to determine the impact of the neutrophil-to-lymphocyte ratio (NLR) and the platelet-to-lymphocyte ratio (PLR) on the prognosis of nasopharyngeal carcinoma (NPC) before and after intensity modulated radiotherapy (IMRT). Pre/post-treatment and changes in inflammatory biomarker levels of 207 patients who were diagnosed with NPC and received IMRT between January 2012 and December 2014 were analyzed, and the cellular biomarker analyses were from patient blood. ROC (receiver operating characteristic) analysis was used to decide the optimal cutoff values of NLR and changes in NLR (ΔNLR) and PLR (ΔPLR). The Kaplan–Meier and logarithmic rank methods were used to compare overall survival times between groups. Univariate analysis was used to investigate the effects of age, gender, histology, Karnofsky performance score (KPS), TNM stage, clinical stage, course of disease and lymphocyte, neutrophil and platelet counts as well as alkaline phosphatase (ALP) levels on the prognosis of NPC. The independent predictors of OS were determined by Cox multivariate regression analysis. The optimal cut-off values of NLR, PLR, ΔNLR and ΔPLR were 2.49, 155.82, 1.80, and 100.00, respectively. These were used to classify patients into high (NLR > 2.49) and low NLR groups (NLR < 2.49); high (PLR>155.82) and low (PLR < 155.82) PLR groups; high (ΔNLR>1.80) and low ΔNLR groups (ΔNLR < 1.80); high (ΔPLR > 100.00) and low ΔPLR groups (ΔPLR < 100.00). TNM stage, clinical stage and ALP levels were highly correlated with high NLR and PLR. Cox multivariate regression analysis suggested that the ΔNLR (HR = 2.89, 95% CI: 1.33∼2.78) was independent of the characteristics for NPC. As a novel inflammatory index, ΔNLR appears to have some predictive power for the prognosis of patients with NPC.
The postinfarction myocardium is in an environment of ischemia and hypoxia, and the damaged myocardium develops into scar tissue lacking electrical conductivity after a series of pathological changes. Thus, the regeneration of blood perfusion and electrical connections is crucial to repair the injured myocardium. In this study, an injectable self-doping conductive hydrogel composed of functionalized carboxymethyl chitosan (CMC) and oxidized hyaluronic acid (OHA) is developed for human umbilical cord mesenchymal stem cell (HUMSC)-derived secretome delivery to protect cardiac function after myocardial infarction (MI). In vitro experiments shows that the conductive hydrogel had good biocompatibility and conductivity consistent with the natural myocardium, and the secretome presents a good ability to promote cell proliferation, migration and angiogenesis. In a rat MI model, echocardiography shows an improvement in ejection fraction and fraction shortening after hydrogel injection. Additionally, susceptibility to arrhythmia is decreased in the standard clinically programmed electrical stimulation (PES) protocol. Immunofluorescence staining demonstrates that hydrogel treatment reduces cardiomyocyte apoptosis and oxidative stress, improves cardiomyocyte morphology and function, promotes angiogenesis. Overall, this study demonstrated the application potential of a conductive hydrogel combined with a cell-free strategy to reconstruct electromechanical coupling in infarcted myocardium and promote cardiac repair and regeneration.
Recent studies have shown that some inflammatory markers are associated with the prognosis of solid tumors. This study aims to evaluate the prognosis of glioma patients with or without adjuvant treatment using the systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), and platelet-lymphocyte ratio (PLR).All patients who were diagnosed with gliomas at the first and second affiliated hospital of Guangxi Medical University between 2011 and 2020 were included in this study. The optimal cutoff value of SII, NLR, and PLR was determined by X-tile software program. We stratified patients into several groups and evaluated the progression-free survival (PFS) and overall survival (OS) of SII, NLR, and PLR during the period of pre-surgical, con-chemoradiotherapy, and post-treatments. Multivariate Cox regression analyses were performed to detect the relationships between OS, PFS, and prognostic variables.A total of 67 gliomas patients were enrolled in the study. The cutoff values of SII, NLR, and PLR were 781.5 × 109/L, 2.9 × 109/L, and 123.2 × 109/L, respectively. Patients who are pre-SII < 781.5 × 109/L had better PFS (P = .027), but no difference in OS. In addition, patients who had low pre-NLR (<2.9 × 109/L) meant better OS and PFS. PLR after adjuvant treatments (post-PLR) was significantly higher than pre-PLR (P = .035). Multivariate analyses revealed that pre-SII, pre-NLR were independent prognostic factors for OS (pre-SII: HR 1.002, 95% CI: 1.000-1.005, P = .030 and pre-PLR: HR 0.983, 95% CI: 0.973-0.994, P = .001), while pre-PLR was an independent factor for PFS (HR 0.989, 95% CI: 0.979-1.000, P = .041).High pre-SII or high pre-NLR could be prognostic markers to identify glioma patients who had a poor prognosis.
The relationship between the consumption of foods with added fructose and non-alcoholic fatty liver disease (NAFLD) was inconsistent in previous epidemiological studies, and no meta-analysis has been performed on the pooled results.
The essay generalizes the study on chemical composition and analysis method of natural active polysacharides, and the clinical application, according to domestic reports in recent years.
This paper reviews the research status of the adverse drug reactions(ADRs)and cause of injection of Herba Houttuyniae.By means of reading its professional paper and periodicals,its ADRs and cause were found,ana- lyzed and summarized.Basic research work on the injection of Herba Houttuyniae should be made as soon as possi- ble,which can make it safe,reliable and effective.At the same time,its standard should be improved perfectly.
To detect the infection of Schistosoma japonicum in mice with a novel test based on agglutination of hybridoma cells and to study the mechanism of the hybridoma cells agglutination.The procedure was developed with a murine cell line H226 producing a monoclonal antibody specific to schistosome 31/32 kDa antigen and sera collected from mice infected with different numbers (10, 30, 50) of S. japonicum cercariae in different period. Immunofluorescent test was carried out with the hybridoma cells and schistosome-infected sera.The circulating antigen was detected by the test as early as 2 weeks after a heavy infection and all mice showed positive results in the test by 5 weeks after infection. The titers of antigen rose along with the time post infection, and the titers of sera from heavy infection were statistically higher than that from the mice receiving a lower number of cercariae. Specific yellowish green fluorescence appeared on the membrane of the hybridoma cells; no signal was detected inside.Hybridoma cell agglutination test (HCAT) may become useful to diagnose schistosomiasis.
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