The measurement characteristics of chemiluminescence nitrogen analyser directly affect the measurement results of total nitrogen content. In order to promote the development of petroleum technology, the corresponding calibration method is studied to ensure the accuracy and reliability of the quantity value. On the basis of fully understanding the structure and working principle of the chemiluminescence nitrogen analyzer, the main measurement parameters were analyzed, and the calibration feasibility was analyzed combined with the corresponding standards and measurement technical specifications. The specific calibration method was determined, which was verified by experiments to provide technical basis for the effective traceability of the chemiluminescence nitrogen analyzer.
The genetic diversity of widely-planted soybean varieties released after 1940s in Northeast China and Yellow-Huai-Hai Rivers Valley(YHH)were analyzed by 125 simple sequence repeats(SSR)markers,which were related to the important traits including yield,quality,stress tolerance and adaptability.The results showed that the average polymorphism information content(PIC)from north part of Heilongjiang province,south and middle parts of Heilongjiang province,Jilin and Liaoning provinces and Yellow-Huai-Hai Rivers Valley were 0.414,0.469,0.522,and 0.562,respectively.Except for the varieties from the north part of Heilongjiang province,the varieties released after 1980 in the other three regions had higher PIC than those released before1980.Based on similarity proposed by Nei and Li,we classified the tested soybean varieties into three major groups by the cluster analysis,corresponding with the original places of the varieties.The varieties from the Northeast and YHH were classified into two major groups respectively,except for Jidou 12,a variety from northern YHH,which was classified into an independent group.In the Northeast,varieties of Heilongjiang were classified into one subgroup and those from Jilin and Liaoning provinces another subgroup,indicating that the widely-planted varieties from the same region were apparently homogenized.
Cotyledon,cotyledonary node and hypocotyl of aseptic seedling of mature black sesame seed were taken as explants,using MS as basic culture medium.There were four kinds of sucrose concentration medium of callus inducement;MS medium with 6-BA,KT,IAA and 2,4-D were formed up as differential medium of sixteen different growth regulator consistencies,the formation and differentiation of callus were researched.The results showed that 30 g/L-cane sucrose concentration is quite advantageous to the callus inducement of cotyledon and cotyledonary node,40 g/L-cane sucrose concentration is quite advantageous to callus inducement of hypocotyl,and the differential medium of MS+ 6-BA 2.0 mg/L+ IAA 1.5 mg/L can make the differenciation frequency of cotyledon callus reach 30.0%,which is the most effective.
Sideband Doppler modulation caused by micro motion of targets on radar echoes is called micro-Doppler effect. Vibration, swing, rotation, coning, precession and nutation are the most common kinds of micro motion. In this paper, micro-Doppler is induced by coning motion mathematically. Then echoes of point scatterers are modelled with linear frequency modulation (LFM) signal. This paper mainly studies the performance of micro-Doppler extracted from echoes of targets with translation compensated completely under different bandwidth. It is more beneficial to extract micro-Doppler characteristics of each scatterer respectively as the bandwidth grows wider, when different scatterers can be separated in range profile domain. At last, the analysis of performance is verified by simulation.
Ectropis grisescens Warren (Lepidoptera: Geometridae) is one of the most severe pests of tea plants in China. This species commonly pupates in soil; however, little is known about its pupation ecology. In the present study, choice and no-choice tests were conducted to investigate the pupation behaviors and emergence success of E. grisescens in response to different substrates (sand, sandy loam 1, sandy loam 2, and silt loam) and moisture contents (5, 20, 35, 50, 65, and 80%). Moisture-choice bioassays showed that significantly more E. grisescens individuals pupated in or on soil (sandy loam 1 and 2 and silt loam) that was at the intermediate moisture levels, whereas 5%- and 35%-moisture sand was significantly more preferred over 80%-moisture sand for pupating. Substrate-choice bioassays showed that sand was most preferred by E. grisescens individuals at 20%- and 80%-moisture levels, but no preference was detected among the four substrates at 50%-moisture content. No-choice tests showed that the percentage of burrowed E. grisescens individuals and pupation depth were significantly lower when soil was dry (20% moisture) or wet (80% moisture). In addition, 20%-moisture sandy loam 2 and silt loam significantly decreased the body water content of pupae and emergence success of adults compared to 50%-moisture content. However, each measurement (percentage of burrowed individuals, pupation depth, body water content, or emergence success) was similar when compared among different moisture levels of sand. Interestingly, pupae buried with 80%-moisture soil exhibited significantly lower emergence success than that were unburied.
Aim To probe into local characters of fractal set. Methods Ordinary density concept is popularized with restricted direction by cone. Results and Conclusion The concept of angular density of fractal sets is given, and its upper and lower bound is discussed.
Objective To study the relation of antineutrophil cytoplasmic antibodies (ANCA) and vasculitis in patients with systemic lupus erythematosus (SLE),and observe their serum tumor necrosis factor alpha (TNF α) and interleukin (IL) 6 level changes.Methods ANCA levels in 60 patients with SLE was measured by indirect immunofluorescence (IIF),and levels of ANCA against myeloperoxidase (MPO) were measured by enzyme linked immunosorbent assay (ELISA).Serum TNF α and IL 6 levels were measured by ELISA in 30 SLE patients and 30 health subjects.Results The prevalence of ANCA was 25% (15/60),which all was perinuclear ANCA (pANCA).MPO ANCA was found in 13 3% of patients (8/66) ,showing that ANCA was associated with certain clinical manifestations of SLE.In particular,the prevalence of ANCA tended to be higher in the patients with a disease duration1 year than with a shorter disease duration ( P 0 05).Positive correlations were found betwen IIF ANCA and lupus nephritis (LN) ( P 0 05),serositis ( P 0 05),skin vasculitis ( P 0 05) and arthritits ( P 0 05).Compared with controls,serum IL 6 and TNF α levels in patients with SLE and in anti MPO ANCA positive group were increased (both P 0 05).Conclusion Above results demonstrate that ANCA is detectable in SLE sera and that some of them are associated with particular clinical manifestations.TNF α and IL 6 are also detected in the sera in association with anti MPO antibodies.Whether ANCA plays a direct pathogenetic role in the vascular damage of SLE or only represents an epiphenomen or a marker of the disease activity remains to be elucidated.
The black-winged subterranean termite, Odontotermes formosanus Shiraki, is a severe pest of plantations and forests in China. This termite cultures symbiotic Termitomyces in the fungal combs, which are challenged by antagonistic microbes such as Trichoderma fungi. In a previous study we showed that O. formosanus workers made significantly fewer tunnels in sand containing commercially formulated conidia of Trichoderma viride Pers. ex Fries compared with untreated sand. Herein, we hypothesize that fungi in the genus Trichoderma exert repellent effects on O. formosanus. Different choice tests were conducted to evaluate the tunneling and aggregation behaviors of O. formosanus workers reacting to sand/soil containing the unformulated conidia of seven Trichoderma fungi (Trichoderma longibrachiatum Rifai, Trichoderma koningii Oud., Trichoderma harzianum Rifai, Trichoderma hamatum (Bon.) Bain, Trichoderma atroviride Karsten, Trichoderma spirale Indira and Kamala, and T. viride). We also investigated the colony-initiation preference of paired O. formosanus adults to soil treated with Trichoderma conidia (T. koningii or T. longibrachiatum) versus untreated soil. Tunneling-choice tests showed that sand containing conidia of nearly all Trichoderma fungi tested (except T. harzianum) significantly decreased tunneling activity in O. formosanus workers compared with untreated sand. Aggregation-choice test showed that T. koningii, T. atroviride and T. spirale repelled O. formosanus workers, whereas T. longibrachiatum and T. hamatum attracted termites. There was no significant difference in proportions of paired adults that stayed and laid eggs in the soil blocks treated with conidia of Trichoderma fungi and untreated ones. Our study showed that Trichoderma fungi generally repelled tunneling in O. formosanus, but may exert varied effects on aggregation preference by workers.
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