Li Jin

Obstetrics and Gynecology Hospital of Fudan University

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Jie Sheng

Shanghai Ocean University

Yishan Song

Shanghai Harbour Engineering Design & Research Institute

Peng Li

Macau University of Science and Technology

Mingfu Wang

Shenzhen University

Beixing Li

Shandong Agricultural University

Junyu Zhang

Lishui Central Hospital

Jing Teng

Tea Research Institute

Feng Zhang

McMaster University

Songchang Chen

Obstetrics and Gynecology Hospital of Fudan University

Jian‐Zhong Sheng

Women's Hospital, School of Medicine, Zhejiang University

Cooperative Institutions

Shanghai Jiao Tong University

Fudan University

Shanghai Ocean University

International Peace Maternity & Child Health Hospital

Chinese Academy of Medical Sciences & Peking Union Medical College

Obstetrics and Gynecology Hospital of Fudan University

Shandong Agricultural University

Zhejiang University

Ministry of Agriculture and Rural Affairs

Ministry of Education of the People's Republic of China

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Evaluation on Synergistic Activity and Resistance Development of the Mixture of Iprodione and Fluopyram Against Botrytis Cinerea

SSRN Electronic Journal (2022)

Fenghua Liu Li Jin Huan Li Kai Wang Meng Lingtao

Gray mold caused by Botrytis cinerea seriously threatens the quality and yield of vegetables or fruits in China. B. cinerea has developed different degrees of resistance to various fungicides because of the extensive and long-term application of fungicides. In this study, the mixture of iprodione and fluopyram was used to control resistant isolates and delay the evolution of resistance. A total of 28 isolates that include sensitive and resistant isolates were selected to determine the combined effect of binary mixtures of iprodione and fluopyram at different proportions, and a binary mixture of iprodione and fluopyram at a ratio of 4:1 (Ipr: Flu [4:1]) presented synergistic effects by 14 isolates of B. cinerea . The optimal proportion of the mixture determined by a variety of isolates showed a significant difference. Next, the mean EC 50 value of 106 isolates collected from different regions in 2021 was 1.84 mg/L for Ipr: Flu (4:1), lower than iprodione (2.41 mg/L) and fluopyram (20.53 mg/L), which indicated the adaptability of the mixture against B. cinerea . Furthermore, The disease suppression of the mixture at 200 mg/L was similar to that of fluopyram at 300 mg/L, which provided the possibility for the application of a mixture with a lower dosage. Finally, UV mutagenesis combined with fungicide-selection experiments was performed to evaluate the risk of resistance development. Compared with iprodione and fluopyram, the binary mixture of iprodione and fluopyram at 4:1 delayed the resistance development of B. cinerea. This study suggested Ipr: Flu (4:1) could enhance disease suppression against sensitive and resistant isolates and delay the resistance development of isolates, which greatly facilitated the application of the mixture of fungicides against B. cinerea and resistance management.

Iprodione

10.2139/ssrn.4118573

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Yili Valley Forage Grass, Straw and Non-conventional Feed Plant Utilization of Resources

Li Jin

Yili river valley is rich combination of grass resources, and it is the guarantee and foundation for animal husbandry sustainable development. In this paper, the forage grass, straw, plant-based unconventional feed resources and its utilization status are analyzed, from the perspective of farmers' income and the sustainable development of animal husbandry, puts forward the opinions and suggestions of efficient utilization of resources.

Animal Husbandry

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Research on Supportive Policy System of Binhai Agricultural Technology Park in Tianjin

Tianjin Agricultural Sciences (2012)

Li Jin

Based on the development status of agricultural technology park in Tianjin Binhai New Area, aiming at the existing issues such as park land, park financing, park mechanism and park technical support at present, this article put forward to integrate and utilize five kinds of park resources that was science and technology resource, land resource, capital resource, information resource and human resource, then to form the making, environment creating,supervision strengthening , risk control overall ideas of policy supporting. The paper has also constructed park policy support system on investment and financing, entrepreneurship and business, tax preference, talents gathering, land supply and industry leading, then proposed innovation ideas and main content of the policy support system.

Investment

Science park

Industrial park

Capital (architecture)

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Prokaryotic expression of cecropin gene isolated from the silkworm Bombyx mori Xinjiang race and antibacterial activity of fusion cecropin

Acta Entomologica Sinica (2004)

Li Jin

In order to clone the cecropin gene from the silkworm Bombyx mori(Xinjiang race) into a T-vector, designed primer was used to amplify 350 bp cecropin cDNA fragment with RT-PCR. Cecropin sequence analysis indicates that the special cecropin fragment of the silkworm Xinjiang race contains entire coding region with 98% identity compared with other reported Bombyx mori cecropin B. The cecropin gene was cloned into expression plasmid pGEX-4T-1 and expressed as fusion protein in Escherichia coli BL21. The results showed that the growth of E.coli contained pGEX-4T-1/cecropin was restrained 30 minutes after adding IPTG and the amount of E.coli began to recover 210 minutes after IPTG induction. This result reveals that the fusion protein has antibacterial activity.

Cecropin

Coding region

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Consideration of Transforming Traditional Agriculture to Modern Agriculture with New High-Technology

Tianjin Nongxueyuan xuebao (2001)

Li Jin

We can learn the connotation of modern agriculture from the distinction between traditional agriculture and modern agriculture.Industrialization of agricultural high technology plays an increasingly important role in transforming traditional agriculture.It must experience four stages of development to achieve industrialization and also have four basic qualifications,that is accurate ability of establishment,capability of factor input,feasibility of high technology and ability of acceptance of farms.The paragraph puts forward the situation of Chinese agricultural high technology industry.It also must coordinate the relation of every carrier of industrialization of agricultural high technology,that is the relation of scientific and technical workers,scientific research institutions,leading enterprises,farms and government.The intervention of government is the leading power of development of the industrialization of agricultural high technology.The relation of industrialization and industrialization operation is the relation of macro layout and micro management.'Scientific research +enterprises +farms'is the basic organization of industrialization and ideal model is to establish compact beneficial communities and develop agricultural high technology enterprises.Tianjin must pay more attention to the development of industrialization agricultural improved variety,industrializatoion of agricultural biologic technology,industrialization of modern equippment,industrialization of information technology and industrialization of agricultural products preservation in the period of the tenth five year program.

Industrialisation

Connotation

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The Changes of Endogenous Hormones in the Shoot of Metasequoia glyptostroboides During Flower Bud Formation

Chih Wu Sheng Li Hsueh T'ung Hsun (1993)

Yuan Fu Li Jin

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Pepper fragrant essential oil (PFEO) and functionalized MCM‐41 nanoparticles: formation, characterization, and bactericidal activity

Journal of the Science of Food and Agriculture (2019)

Li Jin Jing Teng Linghao Hu Xiu-Ting Lan Yue Xu

Abstract BACKGROUND It is well known that plant essential oils have good antimicrobial activity. However, their strong volatility and intense odor limit their application. Mesoporous silica (MCM‐41), a non‐toxic mesoporous material with excellent loading capability, is a promising delivery system for different types of food ingredients in the food industry. RESULTS In this study, we first performed component analysis of pepper fragrant essential oil (PFEO) by gas chromatography – mass spectrometry (GC–MS), then the MCM‐41 host was prepared, and the essential oil functionalized nanoparticles (EONs) were formed by embedding PFEO into mesoporous silica particles. Further analysis indicated that the particle size and zeta potential of EONs were 717 ± 13.38 nm and − 43.90 ± 0.67 mV, respectively. Transmission electron microscopy (TEM) images showed that EONs had an inerratic morphology and stable structure. The bactericidal activities of PFEO and EONs against Escherichia coli ( E. coli ), Salmonella enterica ( S. enterica ), Staphylococcus aureus ( S. aureus ) and Listeria monocytogenes ( L. monocytogenes ) were subsequently tested using the twofold dilution method. Results indicated that, after 48 h incubation, minimum bactericidal concentrations (MBC) of EONs used against gram‐negative bacteria were decreased to a greater degree than those of PFEO, suggesting that nanoencapsulation by MCM‐41 can improve antimicrobial activity. Atomic force microscopy (AFM) observation also confirmed that EONs showed a notable inhibitory effect against E. coli by disrupting cell membrane structure. CONCLUSION Pepper fragrant essential oil nanoencapsulation could be a very promising organic delivery system in food industry for antimicrobial activity enhancement. © 2019 Society of Chemical Industry

Zeta potential

Minimum bactericidal concentration

Salmonella enterica

10.1002/jsfa.9776

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Effects of Ammonium and Nitrate Nitrogen on Growth and Properties of ~(15)N Distribution of Apple Trees

Zhongguo nongye Kexue (2013)

Li Jin

【Objective】 The study was carried out to investigate the growth and properties of15N allocation and distribution in field grown apple trees with dwarfing interstock. 【Method】 The effectiveness of different forms of ammonium and nitrate nitrogen on growth and properties of15N distribution of apple trees were studied by using15N tracer on 1-year-old apple tree(Fuji/SH6/Malus hupehensis Pamp Rehd.).【Result】The results showed that 46 days after fertilization the utilization rate of15NO3-15N was 3.43%, which significantly higher than15NH4-15N, and also the growth vigor and root-shoot ratio. The15NO3-15N partition ratio of base stock was 27.91%, which was also significantly higher than15NH4-15N partition ratio of base stock of25.13%. The nitrogen efficiency of NO3--N reduced till 118 days after fertilization, the growth vigor was significantly lower than NH4+-N,and there was no significant difference between the utilization rate of15NO3-15N and15NH4-15N.15NO3-15N partition ratio of base stock(31.19%) was still significantly higher than15NH4-15N partition ratio of base stock(25.10%). 【Conclusion】Fertilizer efficiency of nitrate nitrogen was much faster than ammonium nitrogen, but there was no advance in long-term cultivation conditions.

Ammonium nitrate

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Preparation of β-cyclodextrin inclusion compound of luteolin

Li Jin

Objective:To prepare β-cyclodextrin inclusion compound of luteolin. Methods: Satu- rated aqueous solution was adapted to achieve inclusion. The inclusion compound obtained was evaluat- ed by IR absorption spectrophotometry, X-ray diffraction technique, and differential scanning calori-metric analysis and its inclusion rate and solubility were also measured. Results: The yield of β-cy-clodextrin inclusion compound of luteolin was 88.32% with a solubility of 88.25mg·L-1(solubility of luteolin=2. 160mg·L-1). Conclusion: The solubility of luteolin is significantly improved by β-cyclodextrin inclusion.

Inclusion compound

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A novel preimplantation genetic testing strategy for a subtelomeric genetic disorder: A case study

Genes & Diseases (2023)

Songchang Chen Li Wang Luting Chen Weihui Shi Junyu Zhang

10.1016/j.gendis.2023.05.013

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