Qingwei Chen

South China Normal University

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Guiqiong Li

Yunnan Agricultural University

Dazhi Ke

Chongqing Medical University

Xingsheng Li

Chongqing Medical University

Li Wang

Vanderbilt University Medical Center

Yipin Zhao

Henan Provincial People's Hospital

Minming Zheng

Chongqing Medical University

Zhongqiu Liu

Guangzhou University of Chinese Medicine

Xiangang Mo

Guiyang Medical University

Liping Wang

Northern Jiangsu People's Hospital

Linlin Lu

Guangzhou University of Chinese Medicine

Cooperative Institutions

Chongqing Medical University

Second Affiliated Hospital of Chongqing Medical University

Dalian Medical University

Sun Yat-sen University

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Shanghai Jiao Tong University

Ministry of Education of the People's Republic of China

Ministry of Agriculture and Rural Affairs

Ningbo University

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Association of fibrinogen/albumin ratio and coronary collateral circulation in stable coronary artery disease patients

Biomarkers in Medicine (2020)

Yipin Zhao Shunli Wang Jiaojiao Yang Zebin Lin Qingwei Chen

Aim: To investigate whether fibrinogen/albumin ratio (FAR) has an association with the coronary collateral circulation (CCC) in patients with stable coronary artery disease. Materials & methods: A total of 391 patients with stable coronary artery disease who underwent coronary angiography were included. The patients were divided into two groups according to the Rentrop score. Results: The poorly developed CCC group had a significantly higher FAR level compared with the well-developed CCC group (p < 0.001). In the multivariate analysis, the FAR (odds ratio: 1.700; 95% CI: 1.420–2.036; p < 0.001) was an independent predictor of poorly developed CCC. Conclusion: FAR can be used as one of the independent predictors of CCC formation.

Collateral circulation

10.2217/bmm-2020-0333

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Citations (12)

Safety and effectiveness of percutaneous coronary intervention (PCI) in elderly patients. A 5-year consecutive study of 201 cases with PCI

Archives of Gerontology and Geriatrics (2010)

Qingwei Chen Yang Yan Ying Liu Dazhi Ke Qing Wu

10.1016/j.archger.2010.01.007

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Citations (13)

[Ghrelin stimulates in vitro angiogenic capacity of rat cardiac microvascular endothelial cells].

PubMed (2012)

Li Wang Qingwei Chen Guiqiong Li Dazhi Ke

To clarify whether ghrelin could promote in vitro rat cardiac microvascular endothelial cells (CMECs) angiogenesis and related mechanisms.CMECs were isolated from myocardial tissue of adult male SD rats and characterized by the immunocytochemistry staining with Factor VIII and the capacity of in vitro capillary tube-like formation. The mRNAs and protein expressions of ghrelin and its receptor (growth hormone secretagogue receptor, GHS-R) of CMECs were determined by RT-PCR, Immunofluorescence, ELISA and Western blot. Proliferation, migration and in vitro angiogenesis as well as ERK2 phosphorylation of CMECs were tested in the presence of ghrelin (10(-9) - 10(-7) mol/L) with or without pretreatment with specific MAPK/ERK2 inhibitor PD98059.Purity of CMECs characterized by immunocytochemistry staining with Factor VIII was about 95%, and the cells showed a high ability to form the capillary tube-like structures on Matrigel. Ghrelin and GHS-R were constitutively expressed in CMECs. Proliferation, migration and in vitro angiogenesis capacities of CMECs (72.20 ± 5.72 vs. 28.60 ± 5.13, P < 0.001; 71.00 ± 7.78 vs. 28.60 ± 5.13, P < 0.001) as well as ERK2 phosphorylation (0.92 ± 0.13 vs. 0.29 ± 0.04, P < 0.001; 1.15 ± 0.16 vs. 0.29 ± 0.04, P < 0.001) were significantly enhanced by exogenous ghrelin (10(-8) - 10(-7) mol/L). PD98059 abolished ghrelin-induced ERK2 phosphorylation and in vitro angiogenesis.Ghrelin and its receptor are expressed in CMECs and ghrelin could stimulate CMECs in vitro angiogenesis through activation of MAPK/ERK2 signaling pathway.

Matrigel

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Characterization of an inositol-producing Lactobacillus plantarum strain and the assessment of its probiotic potential and antibacterial activity

LWT (2021)

Bing Fu Xingxing Huang Jian Ma Qingwei Chen Qili Zhang

The compound inositol has many important applications in various fields. However, no information can be obtained on the biosynthesis of inositol by lactic acid bacteria (LAB) so far. In this study, an inositol-producing Lactobacillus plantarum ZJ2868 was isolated and identified, and its probiotic potential and antibacterial activity were assessed. The isolated L. plantarum ZJ2868 strain produced 33.66 ± 0.75 mg/L of inositol. The optimal temperature and pH for the strain growth were 30 °C and 6.0, respectively. This strain could resist high osmotic pressure, and grew well in Man Rogosa Sharpe (MRS) medium with 6% NaCl. It displayed a strong acid resistance, and the number of viable cells was over 93.9% on the conditions of pH 3.0 and 3.5 for 4 h. 59.8% cells could survive at a high bile salt concentration of 0.1% for 1 h. This strain exhibited an obvious inhibitory effect on Pseudomonas fluorescens and Listeria monocytogenes, and was sensitive to amoxicillin and ampicillin. These findings broaden the use of LAB and deepen our understanding of LAB as potential producer of inositol.

Strain (injury)

Pseudomonas fluorescens

10.1016/j.lwt.2021.112553

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Citations (19)

Enhancing the biosynthesis of riboflavin in the recombinant Escherichia coli BL21 strain by metabolic engineering

Frontiers in Microbiology (2023)

Bing Fu Junhui Ying Qingwei Chen Qili Zhang Jiajie Lu

In this study, to construct the riboflavin-producing strain R1, five key genes, ribA, ribB, ribC, ribD, and ribE, were cloned and ligated to generate the plasmid pET-AE, which was overexpressed in Escherichia coli BL21. The R1 strain accumulated 182.65 ± 9.04 mg/l riboflavin. Subsequently, the R2 strain was constructed by the overexpression of zwf harboring the constructed plasmid pAC-Z in the R1 strain. Thus, the level of riboflavin in the R2 strain increased to 319.01 ± 20.65 mg/l (74.66% increase). To further enhance ribB transcript levels and riboflavin production, the FMN riboswitch was deleted from E. coli BL21 with CRISPR/Cas9 to generate the R3 strain. The R4 strain was constructed by cotransforming pET-AE and pAC-Z into the R3 strain. Compared to those of E. coli BL21, the ribB transcript levels of R2 and R4 improved 2.78 and 3.05-fold, respectively. The R4 strain accumulated 437.58 ± 14.36 mg/l riboflavin, increasing by 37.17% compared to the R2 strain. These results suggest that the deletion of the FMN riboswitch can improve the transcript level of ribB and facilitate riboflavin production. A riboflavin titer of 611.22 ± 11.25 mg/l was achieved under the optimal fermentation conditions. Ultimately, 1574.60 ± 109.32 mg/l riboflavin was produced through fed-batch fermentation with 40 g/l glucose. This study contributes to the industrial production of riboflavin by the recombinant E. coli BL21.

Strain (injury)

Metabolic Engineering

10.3389/fmicb.2022.1111790

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Progress in Transplantation Strategy of Endothelial Progenitor Cells

Advances in Cardiovascular Diseases (2011)

Qingwei Chen

Endothelial progenitor cells,the precursors of endothelial cells,are stem cells that are associated with angiogenesis in adult individuals.These progenitor cells have broad clinical applications in the treatment of ischemic diseases and vascular injury as revealed both by animal experimentation and clinical trials.This article reviews the progress in transplantation strategies of endothelial progenitor cells and their biological characteristics.

Progenitor

Endothelial progenitor cell

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Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model

Peptides (2017)

Li Wang Qingwei Chen Dazhi Ke Guiqiong Li

Monocyte

10.1016/j.peptides.2017.01.013

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Citations (32)

Seven new 1-oxygenated cholestane glycosides from Ornithogalum saundersiae

Journal of Asian Natural Products Research (2019)

Qingwei Chen Ting Gong Pei‐Cheng Zhang Jian‐Qiang Kong

As the continuous scientific research, seven new 1-oxygenated cholestane glycosides named osaundersiosides 1 A - 1 G were isolated from an EtOH extract of the bulbs of Ornithogalum saundersiae. Their structures were deduced by means of spectroscopic data, chemical evidence and the results of hydrolytic cleavage. The cytotoxicity and anti-inflammatory effects of osaundersiosides 1 A - 1 G were evaluated, but none of them displayed significant activities. [Formula: see text].

Materia medica

Cholestane

10.1080/10286020.2019.1656617

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Citations (6)

The effects and mechanisms of ghrelin upon angiogenesis in human coronary artery endothelial cells under hypoxia

Peptides (2022)

Li Wang Qingwei Chen Jun Pang

Angiopoietin receptor

10.1016/j.peptides.2022.170921

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Citations (5)