Pulp regeneration is to replace the inflamed/necrotic pulp tissue with regenerated pulp-like tissue to rejuvenate the teeth. Self-assembling peptide hydrogels RADA16-I (Ac-(RADA16-I)4-CONH2) can provide a three-dimensional environment for cells. The stem cell factor (SCF) plays a crucial role in homing stem cells. Combining these advantages, our study investigated the effects of SCF-RADA16-I on adhesion, proliferation, and migration of human dental pulp stem cells (DPSCs) and the angiogenesis of human umbilical vein endothelial cells (HUVECs). The β-sheet and grid structure were observed by circular dichroism (CD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Cytoskeleton staining, living cell staining, cell viability, cell migration, angiogenesis, and western blot assays were performed, and the results indicated that all the SCF groups were superior to the corresponding non-SCF groups in cell adhesion, proliferation, migration, and angiogenesis. RADA16-I provided a three-dimensional environment for DPSCs. Besides, the SCF promoted HUVECs to form more vascular-like structures and release more vascular endothelial growth factor A. In summary, the SCF-loaded RADA16-I scaffold improved adhesion, proliferation, and migration of DPSCs and the formation of more vascular-like structures of HUVECs. SCF-RADA16-I holds promise for guided pulp regeneration, and it can potentially be applied widely in tissue engineering and translational medicine in the future.

Self-assembling peptide

10.1021/acsomega.0c01266

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Citations (27)

Double Crosslinked Biomimetic Composite Hydrogels Containing Topographical Cues and Way-316606 Induce Neural Tissue Regeneration and Functional Recovery after Spinal Cord Injury

SSRN Electronic Journal (2022)

Jia Liu Xingchang Zhao Xianzhe Lu Kai Li Shiqiang Song

Spinal cord injury (SCI) is an overwhelming and incurable disabling condition, for which increasing forms of multifunctional biomaterials are being tested, but with limited progression. The promising material should be able to fill SCI-induced cavities and direct the growth of new neurons, with effective drug loading to improve the local micro-organism environment and promote neural tissue regeneration. In this study, a double crosslinked biomimetic composite hydrogel comprised of acellularized spinal cord matrix (ASCM) and gelatin-acrylated-β-cyclodextrin-polyethene glycol diacrylate (designated G-CD-PEGDA) hydrogel, loaded with WAY-316606 to activate canonical Wnt/β-catenin signaling, and reinforced by a bundle of three-dimensionally (3D) printed aligned polycaprolactone (PCL) microfibers, was constructed. The G-CD-PEGDA component endowed the composite hydrogel with a dynamic structure with a self-healing capability which enabled cell migration, while the ASCM component promoted neural cell affinity and proliferation. The diffusion of WAY-316606 could recruit endogenous neural stem cells (NSCs) and improve neuronal differentiation. The aligned PCL microfibers guided neurite elongation in the longitudinal direction. Animal behavior studies further showed that the composite hydrogel could significantly recover the motor function of rats after SCI. This study provides a proficient approach to produce a multifunctional system with desirable physiological, chemical, and topographical cues for treating patients with SCI.

10.2139/ssrn.4196058

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Comparative evaluation of a biomimic collagen/hydroxyapatite/β-tricaleium phosphate scaffold in alveolar ridge preservation with Bio-Oss Collagen

Frontiers of Materials Science (2016)

Tong Wang Qing Li Guifeng Zhang Gang Zhou Xin Yu

10.1007/s11706-016-0333-0

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Citations (5)

The Application of Stem Cell Sheets for Neuronal Regeneration after Spinal Cord Injury: A Systematic Review of Pre-Clinical Studies

Luchun Xu He Zhao Yongdong Yang Wenqing Zhong Guozheng Jiang

Participant or population All animal models with spinal cord injury are included without restriction on animal species and modeling methods.Intervention Stem cell sheets. Comparator Stem cell suspension injection

Animal model

Animal studies

10.37766/inplasy2023.7.0028

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全体式外圏自己潤滑関節軸受スピニング成形プロセスの検討【JST・京大機械翻訳】

Xiandai Zhizao Gongcheng (2018)

Xingmin Wang Xiumei Wang Linhui Luo Liu Hongyu Linlin Zhu

Source

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Regulation of MHC Class II and Interleukin-1 Gene Expression in Differentiating Macrophages

Springer eBooks (1987)

Lawrence B. Schook Jeffrey K. Pullen Mihael J. Myers Elizabeth P. Eustis-Turf Xiumei Wang

Northern blot

10.1007/978-1-4757-0764-9_28

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Research on the Spatial Network Structure and Influencing Factors of the Allocation Efficiency of Agricultural Science and Technology Resources in China

Agriculture (2021)

Yameng Wang Zhe Chen Xiumei Wang Mengyang Hou Wei Feng

The allocation efficiency of China’s agricultural science and technology resources (ASTR) varies in different regions and has a complicated spatial distribution pattern. To visually study whether there are correlations and mutual influences between the allocation efficiency of different regions, we use social network analysis methods (SNA). The study found that: (i) China’s allocation efficiency of ASTR has significant spatial correlation and spillover effects. The overall network density is declining. (ii) The spatial correlation network has significant regional heterogeneity. Some eastern provinces play “intermediaries” and “bridges” in the network. (iii) Geographical proximity, differences in economic development levels, industrial structure levels, and differences in urbanization have a significant impact on the formation of spatial association networks.

Spillover effect

Spatial heterogeneity

10.3390/agriculture11111170

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Genome-wide identification and expression of CYP71 gene family in response to low-temperature stress in banana

The Journal of Horticultural Science and Biotechnology (2022)

Chengyu Zhang Shanshan Ni Shuting Zhang Nigarish Munir Chunzhen Cheng

CYP71 belongs to cytochrome P450 (CYP) monooxygenase superfamily, which is functionally diverse and participates in shikimic acid pathway and the secondary metabolism of tryptophan. We identified 58 MaCYP71s and 14 MbCYP71s from the Musa acuminata Colla and Musa balbisiana Colla, and investigated the biological function of the banana CYP71 gene family. Analysis of conserved domain motifs showed that MaCYP71 was more conservative than MbCYP71. Through cluster analysis, MaCYP71 could be divided into 11 subgroups, and MbCYP71 could be divided into 4 subgroups. The expansion of the MaCYP71s might result from the combined actions of tandem and segmental duplications 17% of the MaCYP71 were found to be potentially responsive to low temperature. Exon skipping was the main alternative splicing events of MaCYP71. According to FPKM value and qPCR analysis of MaCYP71 at low temperature, the expressions of MaCYP71A1-4, MaCYP78A6-2, MaCYP78A5-5, and MaCYP78A6-3 at 4°C and 0°C were significantly higher than those at 28°C, which reflected their possible association with response to low-temperature stress. Fifteen members of MaCYP71 family were mainly regulated by miRNA (miR398 and novel_7. 15). MaCYP71s were targeted by 26 lncRNA genes, of which 13 lncRNAs were upregulated in expression at 4°C. From the above studies, it was suggested that MaCYP71 play a vital role in response to low-temperature stress in banana.

Musa acuminata

10.1080/14620316.2022.2098179

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