This chapter contains sections titled: Characteristics and Compatibility Principles of Traditional Chinese Medicine (TCM) Key Scientifi c Issues in TCM Modernization Development of Systems Biology Chemomics Integrated Systems Biology Research Strategy and Prospective of Systems Biology in TCM References
This chapter contains sections titled: Introduction Toxicity Study of Chansu Chemomics Study of LSP Assessment of Effectiveness and Safety of LSP In Vivo Distribution and Metabonomics of LSP and Xionghuang Metabonomic Research of LSP Conclusions References
Zishen Yutai Pills (ZYP) is a safe and well quality-controlled TCM preparation with promising effects in many fields of reproduction, including prevention of miscarriage, increase of pregnancy rate during in vitro fertilization-embryo transfer (IVF-ET). The plasma of patients was collected from a clinical trial, namely, “Effect of Traditional Chinese Medicine vs placebo on live births among women undergoing in vitro fertilization, a multi-center randomized controlled trial.” Plasma samples were analyzed with metabonomics method. UPLC-MS technology was used to establish the plasma metabolic fingerprint. Multivariate statistical analysis was applied for comparing the differences of plasma metabolites between ZYP group and placebo group, 44 potential metabolites were screen out and identified. Pathway analysis was conducted with database mining. Compared with placebo, chemicals were found to be significantly down-regulated on HCG trigger day and 14 days after embryo transplantation, including trihexosylceramide (d18:1/26:1), glucosylceramide(d18:1/26:0), TG(22:6/15:0/22:6), TG(22:4/20:4/18:4). Compared with placebo, some chemicals were found to be significantly up-regulated on HCG trigger day and 14 days after embryo transplantation, i.e., PIP3(16:0/16:1), PIP2(18:1/18:1), tauroursodeoxycholic acid, L-asparagine, L-glutamic acid, kynurenic acid, 11-deoxycorticosterone, melatonin glucuronide, hydroxytyrosol. These metabolites were highly enriched in pathways including sphingolipid metabolism, alanine, aspartic acid and glutamic acid metabolism, aminoacyl tRNA biosynthesis, taurine and hypotaurine metabolism. This study revealed metabolic differences between subjects administered with ZYP and placebo. Relating metabolites were identified and pathways were enriched, providing basis on the exploration on the underlying mechanisms of ZYP combined with IVF-ET in the treatment of infertility.
A rapid, sensitive, and specific high-performance liquid chromatography electrospray tandem mass spectrometric method has been developed for the determination of levonorgestrel in human serum using norethindrone as an internal standard. Levonorgestrel was detected using multiple reaction monitoring mode after extracted from human serum by an ether extraction procedure. The calibration curve was linear over the range of 5–500 ng/mL (r 2 ≥ 0.999) with the limitation of detection of 1 ng/mL. The intra-day precision and accuracy, expressed as CV and RE, ranged from 0.5% to 10.1% and −0.2% to 2.2%. The inter-day precision and accuracy ranged from 2.1% to 13.6% and −2.3% to 3.3%. The mean recovery was 89.5% for levonorgestrel, and 90.2% for the internal standard.
Abstract A novel asymmetrical cyanine dye (TR) with NIR absorption at 970 nm was synthesized and applied in photoacoustic imaging (PAI) in vivo for the first time. The advantages of asymmetrical structure in PAI was demonstrated with DFT calculation and verified by related experiments. BSA was selected as the matrix to encapsulate TR‐BSA nanoparticles (TR‐NPs) which was investigated as a PAI agent. Exceptional performance of TR‐NPs was shown in Balb/c rats caudal vein model; the details could be clearly displayed and identified at a 0.1 mm scale.
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