Zhenfeng Wang

People’s Hospital of Linqing

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Deming Wang

Shanghai Jiao Tong University

Lixin Su

Shanghai Jiao Tong University

Xi Yang

Anhui Medical University

Caifeng Ding

Qingdao University of Science and Technology

Mingzhe Wen

Shanghai Jiao Tong University

Xin-yu Li

Guangzhou University of Chinese Medicine

Chao Li

Changchun Normal University

Tingbang He

Shandong First Medical University

Deguo Yu

People’s Hospital of Linqing

Feng Wang

Zunyi Medical University

Cooperative Institutions

Shanghai Jiao Tong University

Chinese Academy of Sciences

Shanghai Ninth People's Hospital

Hefei University of Technology

University of Chinese Academy of Sciences

Shandong First Medical University

Sichuan University

Central South University

Suzhou University of Science and Technology

Peking University

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Fluidity-Guided Assembly of Au@Pt on Liposomes as a Catalase-Powered Nanomotor for Effective Cell Uptake in Cancer Cells and Plant Leaves

ACS Nano (2022)

Zhenfeng Wang Yong Yan Chao Li Yue Yu Sheng Cheng

The fluidity of the liposomes is essential to nanoparticle–membrane interactions. We herein report a liposomal nanomotor system by controlling the self-assembly behavior of gold core–platinum shell nanoparticles (Au@Pt) on liposomes. Au@Pt can aggregate immediately on fluid-phase dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes, forming an uneven distribution. By control of the lipid phase and fluidity, either using pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) above its phase transition temperature or adding cholesterol as an adjuvant to DPPC lipids, we precisely control the assembly of Au@Pt on liposomes. Au@Pt maintained high catalase-like activity on the liposomal surface, promoting the decomposition of H2O2 and the movement of the liposomal nanomotors. Finally, we demonstrate that liposomal nanomotors are biocompatible and they can speed up the cellular uptake in mammalian HepG2 cancer cells and Nicotiana tabacum (Nb) plant leaves. This liposomal nanomotor system is expected to be further investigated in biomedicine and plant nanotechnology.

10.1021/acsnano.2c00327

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The relationship between interleukin-1 gene polymorphism and the serum level of interleukin-1β and the multiple sclerosis patients in the Northeast of China

Journal of Apoplexy and Nervous Diseases (2006)

Zhenfeng Wang

Objective To implore the relationship between interleukin-1,especially interleukin-1β(IL-1β) and interleukin-1 receptor antagonist(IL-1RA)gene polymorphism and the serum level of IL-1β and the multiple sclerosis(MS)patients in the Northeast of China. Methods DNA of white blood cells from 54 subjects with MS and 60 healthy controls was extracted.Polymorphisms in IL-1β and IL-1RA were analyzed with PCR in order to find the difference of the genotype distribution and the allele frequency between the MS patients and the healthy controls.Results The genotype(CC,CT,TT)distribution of IL-1β was 44.4%,22.2%,and 33.4% in patients with MS and 46.7%,23.3%,and 30.0% in controls(P(0.05));while for the IL-1RA(I/I,I/II,II/II,I/IV) was 81.5%,7.4%,5.6%,5.5% in MS patients and 80.0%,8.3%,5.0%,6.7% in controls(P(0.05)).The allele frequency of IL-1β in patients with MS were C 61.1%,T 38.9% and 60.0%,40.0% in controls;while for the IL-1RA(I,II,IV) were 85.2%,9.3%,5.5% in MS patients and 83.3%,10.0%,6.7% in controls.(P(0.05)).The serum production of IL-1β was 146.83pg/ml in MS patients and was 96.23pg/ml in controls(P(0.05)).Conclusion The IL-1β and IL-1RA polymorphisms were not significantly associated with the susceptibility of MS,while IL-1β maybe play an active role in the MS and be involved in the start of inflammation progression and tissue damage.

Gene polymorphism

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Single-cell and spatial transcriptomic analyses reveal transcriptional cell lineage heterogeneity in extracranial arteriovenous malformation

Journal of Dermatological Science (2025)

Yi Eve Sun Haoyang Xu Yafang Zhu Yamin Rao Xindong Fan

Lineage (genetic)

Spatial heterogeneity

10.1016/j.jdermsci.2025.02.005

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Apatinib plus paclitaxel versus paclitaxel monotherapy for platinum‐resistant recurrent ovarian cancer treatment: A retrospective cohort study

Journal of Clinical Pharmacy and Therapeutics (2022)

Aizhi Geng Hailei Yang Zhenfeng Wang Chuanzhong Wu

Apatinib, an oral antiangiogenic drug, exerts potential anti-tumour effects on platinum-resistant recurrent ovarian cancer (PROC). This study intended to evaluate the efficacy and safety of apatinib plus paclitaxel compared to paclitaxel monotherapy in PROC patients.This retrospective cohort study reviewed 70 PROC patients who received apatinib plus paclitaxel (apatinib plus paclitaxel group) (N = 32) or paclitaxel monotherapy (paclitaxel monotherapy group) (N = 38). The recommended regimens were as follows: paclitaxel (60 mg/m2 ) administrated once a week with a maximum of 18 weeks; apatinib (250-375 mg/day) administrated until disease progression or patient intolerance.Disease control rate was elevated (84.4% vs. 60.5%, P = 0.028), whereas objective response rate only disclosed an increasing trend (lacked statistical significance) (37.5% vs. 18.4%, P = 0.074) in apatinib plus paclitaxel group compared with paclitaxel monotherapy group. Progression-free survival (median [95% confidence interval (CI)]: 5.0 [2.5-7.5] months vs. 3.8 [2.4-5.2] months, P = 0.033) and overall survival (median [95% CI]: 21.1 [13.2-29.0] months vs. 14.8 [11.4-18.2] months, P = 0.032) were both prolonged in apatinib plus paclitaxel group compared to paclitaxel monotherapy group, which were further verified in the multivariate Cox's proportional hazard regression analyses (both P < 0.050). Additionally, the incidence of each adverse event was not different between the two groups (all P > 0.050).Apatinib plus paclitaxel exhibits better efficacy and acceptable toxicity compared with paclitaxel monotherapy in PROC patients.

Apatinib

10.1111/jcpt.13806

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Ultrasensitive chemiluminescence quantification of single-nucleotide polymorphisms by using monobase-modified Au and CuS nanoparticles

Biosensors and Bioelectronics (2009)

Caifeng Ding Zhenfeng Wang Hua Zhong Shusheng Zhang

Klenow fragment

10.1016/j.bios.2009.09.034

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Citations (35)

Ferric Ions as a Catalytic Mediator in Metal‐EGCG Network for Bactericidal Effect and Pathogenic Biofilm Eradication at Physiological pH

Advanced Materials Interfaces (2021)

Shuai Chen Yong Yan Yue Yu Zhenfeng Wang Xiaojun Zhu

Abstract Pathogenic bacteria and biofilm formation have become the urgent threats to global public health. Herein, metal‐epigallocatechin gallate (EGCG) network with improved bactericidal effect and pathogenic biofilm eradication ability via trivalent cationic Fe 3+ catalyzed hydrogen peroxide (H 2 O 2 ) decomposition is developed. EGCG, the predominant catechin from tea, is predicted to be a good chelator for eight metal ions (i.e., Al 3+ , Cr 3+ , Cu 2+ , Co 2+ , Zn 2+ , Mn 2+ , Ni 2+ , Fe 3+ ). 3,3',5,5'‐tetramethylbenzidine oxidation experiment using UV–vis spectrometer demonstrated that Zn 2+ , Mn 2+ , Ni 2+ are the potential candidates to accelerate the generation of H 2 O 2 by EGCG. Compared with the other two metal‐EGCG network (Zn 2+ ‐EGCG or Mn 2+ ‐EGCG), only Ni 2+ ‐EGCG network has better bactericidal property and plays a synergistic role in the network system. Furthermore, by introducing Fe 3+ to Ni 2+ ‐EGCG network, the complex exhibits the best bactericidal property for gram‐negative bacteria Escherichia coli ( E. coli ) and Pseudomonas aeruginosa (PAO1), which can be explained by the rapid H 2 O 2 decomposition and associated production of hydroxyl radicals. With SYTO 9/PI staining assay, Fe 3+ as a catalytic mediator in Ni 2+ ‐EGCG network shows the most significant bactericidal effect and pathogenic biofilm eradication ability. According to the above analysis, Ni 2+ ‐EGCG network presents a promising nanoplatform as an alternative to antibiotics for eradication of pathogenic bacteria and biofilms.

Gallate

Pathogenic bacteria

Minimum bactericidal concentration

10.1002/admi.202101605

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Clinical Implications of Necroptosis Genes Expression for Cancer Immunity and Prognosis: A Pan-Cancer Analysis

Frontiers in Immunology (2022)

Xin-yu Li Lixin Su Wenxue Chen Hui Liu Luyu Zhang

Necroptosis, a form of programmed cell death, is increasingly being investigated for its controversial role in tumorigenesis and progression. Necroptosis suppresses tumor formation and tumor development by killing tumor cells; however, the necrotic cells also promote tumor formation and tumor development via the immunosuppressive effect of necroptosis and inflammatory response caused by cytokine release. Thus, the exact mechanism of necroptosis in pan-cancer remains unknown.The data of 11,057 cancer samples were downloaded from the TCGA database, along with clinical information, tumor mutation burden, and microsatellite instability information of the corresponding patients. We used the TCGA data in a pan-cancer analysis to identify differences in mRNA level as well as single nucleotide variants, copy number variants, methylation profiles, and genomic signatures of miRNA-mRNA interactions. Two drug datasets (from GDSC, CTRP) were used to evaluate drug sensitivity and resistance against necroptosis genes.Necroptosis genes were aberrantly expressed in various cancers. The frequency of necroptosis gene mutations was highest in lung squamous cell carcinoma. Furthermore, the correlation between necroptosis gene expression in the tumor microenvironment and immune cell infiltration varied for different cancers. High necroptosis gene expression was found to correlate with NK, Tfh, Th1, CD8_T, and DC cells. These can therefore be used as biomarkers to predict prognosis. By matching gene targets with drugs, we identified potential candidate drugs.Our study showed the genomic alterations and clinical features of necroptosis genes in 33 cancers. This may help clarify the link between necroptosis and tumorigenesis. Our findings may also provide new approaches for the clinical treatment of cancer.

Tumor progression

10.3389/fimmu.2022.882216

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Citations (4)

Glutathione-Sensitive Nanoglue Platform with Effective Nucleic Acids Gluing onto Liposomes for Photo-Gene Therapy

ACS Applied Materials & Interfaces (2022)

Yue Yu Zhenfeng Wang Sichen Wu Chunmeng Zhu Xianshe Meng

Liposomal spherical nucleic acids possess a high density of nucleic acids, e.g., DNA, on a liposomal core. There are two approaches to conjugate DNA onto the zwitterionic liposomes, i.e., covalent and noncovalent conjugation, otherwise using cationic liposomes. However, complex and expensive DNA chemical modification methods need to seek a novel and easy-operating approach to decorating DNA onto liposomes. Inspired by the nanoparticle solution as nanoglues for gels and biological tissues, we use MnO2 nanosheets to "glue" DNA onto liposomes without DNA modification. In tumor cells with a high glutathione concentration, MnO2-based nanoglues are degraded, generating water-soluble Mn2+ ions, further "unglue" DNA (i.e., DNAzyme), and liposomes. Using the intelligent liposomal nanoglue (DNAzyme/MnO2/Lip) combining glutathione-sensitive MnO2 nanosheets, gene silencing agent DNAzyme, and photosensitizer Chlorin e6 (Ce6) in liposomes, effective photo-gene therapy was demonstrated.

Deoxyribozyme

Conjugate

10.1021/acsami.2c04022

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Citations (12)

Screening and functional identification of antioxidant microRNA-size sRNAs from

Functional Plant Biology (2021)

Dechao Chen Shuya Chen Chenxi Zhao Jin Yan Zelong Ma

MiRNA-size small RNAs, abbreviated as sRNAs, are increasingly being discovered as research progresses and omics technologies development in prokaryotes. However, there is a paucity of data concerning whether or not sRNAs exist in cyanobacteria and regulate the resistance to oxidative stress. In this investigation, small RNA libraries were constructed from the control, 50-nM and 100-nM H2O2 treatments of Spirulina platensis. By high-throughput sequencing, 23 candidate sRNAs showed significantly differential expression under oxidative stress, among which eight sRNAs were identified with the similar expression patterns as the sequencing results by real-time qPCR. By nucleic acid hybridisation, the corresponding expression changes also demonstrated that sequencing results of sRNAs were feasible and credible. By bioinformatics prediction and structure identification, 43 target genes were predicted for 8 sRNAs in plant miRNA database, among which 29 were annotated into the genome and related metabolic pathways of S. platensis. By COG functional classification and KEGG pathway analysis, 31 target genes were predicted to be directly or indirectly involved in the defence mechanism of H2O2 stress. Thirteen target genes displayed reversely changing patterns compared with those of their sRNAs under H2O2 treatment. These findings provide compelling evidence that these sRNAs in S. platensis play a crucial role in oxidative stress responses, and thus provide a theoretical reference for improving the stress-triggering physiological regulation.

KEGG

Identification

10.1071/fp20405

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Citations (1)

Rolling circle amplification combined with nanoparticle aggregates for highly sensitive identification of DNA and cancercells

Biosensors and Bioelectronics (2012)

Caifeng Ding Nannan Wang Jian Zhang Zhenfeng Wang

Differential pulse voltammetry

Rolling circle replication

Primer (cosmetics)

Aptamer

10.1016/j.bios.2012.10.015

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Citations (20)