Abstract Background Increasing evidence suggests that ADSCs execute their paracrine function via the secretion of exosomes, especially under hypoxic conditions. However, the mechanisms by which ADSCs-derived exosomes (ADSC-exos) enhance angiogenesis under hypoxia remain unclear. Methods Exosomes were isolated from HIF-1α-modified ADSCs culture supernatants. To investigate the effects HIF-1α-ADSC-exos on HUVECs, cell growth, apoptosis, and tube formation assay were performed with or without HIF-1α-ADSC-exos. Moreover, to determine the function of HIF-1α-ADSC-exos, the therapeutic effects of ADSC-exos and HIF-1α-ADSC-exos were examined in PAH rats. Results Exosomes released by HIF-1α-modified ADSCs rescued the impaired angiogenic ability, migratory function, and inflammatory factors of hypoxia-injured HUVECs, with increased SDF-1α, Rac1, Rac2, VEGF and IL-10 expression. Furthermore, exos-HIF-1α activated SIRT3 to enhance angiogenesis in HUVECs and induced IL-10 expression to inhibit inflammatory response. Block SIRT3 or SDF-1α abolished the angiogenic effect in HUVECs. Conclusion Our findings indicated that the SIRT3 contributed a crucial role in HIF-1α-ADSC-exos in tissue repair under hypoxia.
OBJECTIVE:To evaluate the status quo and trend of the application of psychotropic drugs in hospitals of Xinjiang area. METHODS: By a retrospective study,the application data and the related indexes of psychotropic drugs in 7 general hospitals of Xinjiang area during 2007～2009 were analyzed statistically. RESULTS: Midazolam injection, Tramadol hydrochloride sustained release tablet and Tramadol hydrochloride injection ranked at the first three places in terms of consumption sum. Estazolam tablets, Alprazolam tablets and Clonazepam tablets ranked at the first three places in terms of DDDs. The DDDs of Tramadol hydrochloride injection decreased gradually. CONCLUSION: The application of psychotropic drugs in Xinjiang area tended to be rational more and more, but clinical use of drugs should be further standardized.
Objective
To study the prognosis, including menstruation, ovarian function and fertility after the treatment of uterine myoma with uterine arterial embolization (UAE).
Methods
From October 2007 to November 2011, a total of 56 patients who were diagnosed as symptomatic uterine myoma through ultrasound, MRI and gynecological examination in Chengdu Second People's Hospital were admitted to this study, excluding endometrial lesions and malignant cervical lesions. All the 56 patients were treated with bilateral uterine artery intubation for UAE therapy. A retrospective analysis was applied to analyze the medical records. Statistic analysis was used to evaluate the volume of uterine myoma and uterine, the menstruation, hormones levels and fertility of the subjects. The study procedure was in accordance with ethical standard of the hospital clinical study ethics committee and approved by the committee. The grouping information was acknowledged by the subject's approval and informed consent forms were signed by every subject.
Results
① After three months of treatment with UAE, the volumes of uterine myoma and uterine both were significantly decreased than those before treatment with UAE, and the differences were statistically significant (P 0.05). ④ After three years of following-up, among the 15 cases of patients with desire for pregnancy, 3 cases showed gestation and labor without preterm labour, miscarriage and other complications of maternal diseases. The average time to get pregancy was 11.5 months after UAE treatment. The fertility rate in the study was 20% (3/15).
Conclusions
UAE is a safe and effective therapy method to uterine myoma, and has minimal effect on the menstruation and fertility. Since the sample size of the current study is quite small, UAE treatment still needs multiple center and large sample size and random case-control study to further clarify the effect on the fertility of the patients with uterine myoma.
Key words:
Myoma, uterus; Artery, uterus; Embolism; Menstruation disturbances; Fertility; Complications
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Abstract Background: Ferroptosis is a peroxidation-driven form of cell death. Glutathione peroxidase 4 (GPX4) and System Xc − are leading regulators of ferroptosis. This study investigated System Xc − and GPX4 ubiquitination in ferroptosis after subarachnoid hemorrhage (SAH). Methods: SAH rat model was established by artery perforation operation. The mortality, neurological score, brain water content and blood brain barrier permeability were and analyzed. The morphology of mitochondria was observed, the content of reactive oxygen species and the pathology of hippocampal neurons were evaluated, and the iron deposition was observed. Levels of ferroptosis-related proteins and proinflammatory factors were detected. The protein interaction between System Xc − and GPX4 was verified. After ferroptosis was induced by FIN56 in neurons, the neurons were transfected with OTUB1 overexpression vector or ubiquitination inhibitors to verify the regulatory mechanism of System Xc − and GPX4 ubiquitination in ferroptosis. Results: After SAH operation, GPX4 was downregulated, and induced iron deposition and ROS accumulation. In SAH rats, System Xc − function was lost, GSH decreased, MDA content and SLC3A2, SLC7A11 and GPX4 ubiquitination increased, and OTUB1 expression decreased. SLC3A2 and OTUB1, GPX4 and OTUB1 can interact with each other in protein. Overexpression of OTUB1 reduced the ubiquitination of SLC3A2, SLC7A11 and GPX4 in FIN56-induced ferroptosis in neurons. Ubiquitination inhibitor or OTUB1 increased GSH level in ferroptotic neurons, and reduced ROS accumulation and inflammatory damage. Conclusion: SAH induced the ubiquitination of System Xc − and GPX4, resulting in GSH depletion, increased lipid oxidative damage, and promoted ferroptosis in hippocampal neurons.
Aim To study the antitumor activity of Dipeptide Lys-Glu (Vilon). Methods Dipeptide Vilon, which molecular weight is 275.3, was synthetized by the liquid-phase synthetic method. Proliferation inhibition of human intestines tumor cell of LOVO, human stomach tumor cell MKN-45 and human liver tumor cell QGY7703 assays were examined by cell accounting and MTT method, and test the influence of healthy human normal leucocytes. Experiments in vivo were tested by treatment on tumor mice. Results Vilon exerted strong dose-dependent inhibition on the proliferation of three tumor cell lines, but not human normal leucocytes. Treatments on tumor mice showed that effectively inhibited tumor growth of mice H22 hepatoma with apparently dose-dependent effect. The most minimum efficient dose was 15 mg·kg-1. When higher dose (30 mg·kg-1) was used, the inhibition rate of mice H22 hepatoma reached above 60%. Conclusion Vilon has apparent antitumor activity in vitro and vivo.
Ramie (Boehmeria nivea L.), belonging to Urticaceae, is principally used for fabric production. It is a well-known natural fiber material for ancient clothing. Despite its important position and application value, the understanding on genetic regulation mechanism of fiber quality is limited. Here, we generate a chromosome-scale, high-quality reference genome of ramie, in which, approximately 90.2% of the assembled sequences have been anchored to 14 pseudochromosomes. Totally 27,664 protein-coding genes are predicted which cover 268.24 Mb region of the genome. Comparative genomic analysis reveals that 2,047 and 796 gene clusters expand and contract, respectively, underlying significant genes in plant hormone signal transduction and cellulose/lignin biosynthesis pathways. An integrative analysis combining quantitative trait loci (QTL), comparative transcriptomic data, and cytological experiments unravels the molecular regulatory mechanism of ramie fiber fineness, especially the critical regulating role of ethylene. This study would lay a solid foundation for the research of molecular biology in ramie and provide valuable reference for the improvement of high-quality fiber varieties.
A novel flow-injection chemiluminescence method was developed for the selective determination of human immunoglobulin G (IgG) in the presence of thiomersal by changing the flow rates of peristaltic pump. The study was based on the independence and additivity of the CL signals of human IgG and thiomersal in the galangin-potassium permanganate-polyphosphoric acid system. In meantime, two equations relating to the concentrations of mixing solutions of human IgG and thiomersal vs the CL intensity were established and solved, on the basis of which the content of thiomersal included in samples was simultaneously determined too. The enhanced CL intensity was in proportion to concentrations in the range 8.0 × 10(-7) to 8.0 × 10(-5) g/mL for human IgG and 1.0 × 10(-7) to 2.0 × 10(-6) g/mL for thiomersal with the detection limits of 5.0 × 10(-7) g/mL for human IgG and 6.0 × 10(-8) g/mL for thiomersal, respectively. The relative standard deviation for 1.0 × 10(-5) g/mL human IgG was 0.8% and for 2.0 × 10(-7) g/mL thiomersal it was 2.0% (n = 10). The proposed method was applied to determine three synthetic samples with recoveries of 91.5-109.5%. In addition, the possible chemiluminescence mechanisms are discussed as well.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)