Wei Liu

Jilin Agricultural University

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Wei Li

Fujian Agriculture and Forestry University

Guangqing Xia

Dalian University of Technology

Mengqiu Zhang

Second Hospital of Anhui Medical University

Yuan-Yuan Liu

Nanjing Traditional Chinese Medicine Hospital

Jingang Hou

Intelligent Synthetic Biology Center

Guo Shankang

Shun Liu

Chinese Academy of Medical Sciences & Peking Union Medical College

Jun‐Jie Zhang

Nanjing Medical University

Lan Wang

Henan University of Science and Technology

Junyi Zhu

Beijing University of Technology

Cooperative Institutions

Nanjing University of Chinese Medicine

Chinese Academy of Medical Sciences & Peking Union Medical College

Jilin University

Jilin Agricultural University

Jiangsu Province Hospital

China Pharmaceutical University

Chinese Academy of Sciences

Tonghua Normal University

Jiangsu Provincial Academy of Traditional Chinese Medicine

Shanghai Jiao Tong University

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A spatially-resolved approach to visualize the distribution and biosynthesis of flavones in Scutellaria baicalensis Georgi

Journal of Pharmaceutical and Biomedical Analysis (2019)

Chenglong Sun Minmin Zhang Hongjing Dong Wei Liu Lanping Guo

Flavones

Baicalin

Scutellaria

10.1016/j.jpba.2019.113014

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Citations (41)

Analysis content on the amino acid, vitamin and chemical element of cerebral cell active factor

Journal of Northeast Normal University (2001)

Wei Liu

The chemical content of injectio cerebral cell active factor, was determined by modern analytic determination methods, inorder to study pharmacologic work better. The content of amino acids vitamins and chemical element was determined by LC-60A shimadzu and P-E3030 made in USA. The drug has 18 kinds of amino acids, 3 kinds of vitamins (not complete) and 20 kinds of chemical elements. The results can provided referential data for improving the method of mixture and sound clinic administration.

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Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS

Current Pharmaceutical Analysis (2019)

Shun Liu Xun Wang Kaiping Zou Wei Liu Cunyu Li

Background: Zishen Tongguan (ZSTG) capsules were prepared at the Affiliated Hospital of Nanjing University of Chinese Medicine and have been proven to be clinically effective for treating pyelonephritis and benign prostatic hyperplasia. However, the quality standards are not ideal; a comprehensive study of the “quality markers” (Q-markers), the chemicals inherent in traditional Chinese medicine and its preparations, has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high-performance liquid chromatographictandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of eight potential Q-markers of ZSTG, including timosaponin A3, berberine, jatrorrhizine, phellodendrine, palmatine, mangiferin, neomangiferin, and timosaponin BII. A Kromasil 100-3.5 C18 column was used with a mobile phase of 0.2% formic acid with acetonitrile, and gradient elution at a flow rate of 0.2 mL/min was achieved in 13 minutes and used for separation. Detection was performed in positive/negative mode with multiple reaction monitoring (MRM). Results: The analytical method was validated in terms of the sensitivity, linearity, accuracy, precision, repeatability, stability and recovery. The method established here was successfully applied to study the potential Q-markers in 8 batches of commercial samples, which demonstrated its use in improving the quality control of ZSTG. Conclusion: The developed method had high repeatability and accuracy and was suitable for the simultaneous analysis of multiple Q-markers, which may provide a new basis for the comprehensive assessment and overall quality control of ZSTG.

Repeatability

Jatrorrhizine

Mangiferin

10.2174/1573412915666190522081113

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Determination of pteroic acid of hydrolysis folic acid by HPLC

Journal of Taishan Medical College (2012)

Wei Liu

Objective: To determine pteroic acid in the samples synthesized in our laboratory by hydrolyzing folic acid.Methods: HPLC method was developed employing reserved-phase chromatographic column Phenomexne C18(250 mm×4.6 mm)and UV detection in the wave length of 285nm.The mobile phase consisted of methanol and water(containing 2% acetic acid,0.2% triethylamine)(25︰75,v/v) with a flow rate of 1 ml/min.Results: The purity of synthesized pteroic acid was about 73%.The method provided a good linearity from 16 μg/mL to 9.6 μg/ml.Relativity coefficient was 0.9991,by the results of stability test RSD was 0.42%.The average recovery was 98.9%.Conclusion: The developed HPLC method is accurate and precise,which can be used for the content determination of pteroic acid.

Triethylamine

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Human D-Tyr-tRNATyr deacylase contributes to the resistance of the cell to D-amino acids

Biochemical Journal (2008)

Gen Zheng Wei Liu Yanhua Gong Hongbo Yang Bin Yin

DTD (D-Tyr-tRNA(Tyr) deacylase) is known to be able to deacylate D-aminoacyl-tRNAs into free D-amino acids and tRNAs and therefore contributes to cellular resistance against D-amino acids in Escherichia coli and yeast. We have found that h-DTD (human DTD) is enriched in the nuclear envelope region of mammalian cells. Treatment of HeLa cells with D-Tyr resulted in nuclear accumulation of tRNA(Tyr). D-Tyr treatment and h-DTD silencing caused tRNA(Tyr) downregulation. Furthermore, inhibition of protein synthesis by D-Tyr treatment and h-DTD silencing were also observed. D-Tyr, D-Asp and D-Ser treatment inhibited mammalian cell viability in a dose-dependent manner; overexpression of h-DTD decreased the inhibition rate, while h-DTD-silenced cells became more sensitive to the D-amino acid treatment. Our results suggest that h-DTD may play an important role in cellular resistance against D-amino acids by deacylating D-aminoacyl tRNAs at the nuclear pore. We have also found that m-DTD (mouse DTD) is specifically enriched in central nervous system neurons, its nuclear envelope localization indicates that D-aminoacyl-tRNA editing may be vital for the survival of neurons under high concentration of D-amino acids.

10.1042/bj20080617

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Diversity and composition of the Panax ginseng rhizosphere microbiome in various cultivation modesand ages

BMC Microbiology (2021)

Ai-Zi Tong Wei Liu Qiang Liu Guangqing Xia Junyi Zhu

Continuous cropping of ginseng (Panax ginseng Meyer) cultivated in farmland for an extended period gives rise to soil-borne disease. The change in soil microbial composition is a major cause of soil-borne diseases and an obstacle to continuous cropping. The impact of cultivation modes and ages on the diversity and composition of the P. ginseng rhizosphere microbial community and technology suitable for cropping P. ginseng in farmland are still being explored.Amplicon sequencing of bacterial 16S rRNA genes and fungal ITS regions were analyzed for microbial community composition and diversity.The obtained sequencing data were reasonable for estimating soil microbial diversity. We observed significant variations in richness, diversity, and relative abundances of microbial taxa between farmland, deforestation field, and different cultivation years. The bacterial communities of LCK (forest soil where P. ginseng was not grown) had a much higher richness and diversity than those in NCK (farmland soil where P. ginseng was not grown). The increase in cultivation years of P. ginseng in farmland and deforestation field significantly changed the diversity of soil microbial communities. In addition, the accumulation of P. ginseng soil-borne pathogens (Monographella cucumerina, Ilyonectria mors-panacis, I. robusta, Fusarium solani, and Nectria ramulariae) varied with the cropping age of P. ginseng.Soil microbial diversity and function were significantly poorer in farmland than in the deforestation field and were affected by P. ginseng planting years. The abundance of common soil-borne pathogens of P. ginseng increased with the cultivation age and led to an imbalance in the microbial community.

Diversity index

10.1186/s12866-020-02081-2

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Stability of the tetramine in blood samples under different temperatures

Journal of Forensic Medicine (2009)

Jun Bu Wei Liu Hui Yan

OBJECTIVE To study the stability of tetramine in blood samples under different environment temperatures. METHODS The tetramine samples in 0.5 microg/mL concentration were stored in the environment temperature at 45 degrees C, 25 degrees C and 4 degrees C. The tetramine concentration were measured by GC-MS in 1, 3, 12, 18 and 39 days, respectively. And then the results of measurement were analyzed by SPSS software. RESULTS At 45 degrees C and 25 degrees C, the concentration of tetramine changed without distinctly in first 3 days and declined significantly from 4 days to 12 days. Thereafter it declined more slowly. At 4 degrees C, the concentration of tetramine changed without distinctly in 12 days and declined significantly from 13 days to 18 days. Thereafter it declined more slowly. CONCLUSION The concentration of tetra mine in blood samples is stable in 3 days. Thereafter it declined gradually. Temperature also influences the concentration of tetra mine.

Tetramine

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Protective Effect of Ginsenosides from Stems and Leaves of Panax ginseng against Scopolamine-Induced Memory Damage via Multiple Molecular Mechanisms

The American Journal of Chinese Medicine (2022)

Ying Wang Jun‐Jie Zhang Jingang Hou Xin Li Wei Liu

Although growing evidence has shown that ginsenosides from stems and leaves of Panax ginseng (GSLS) exercise a protective impact on the central nervous system, in the model of memory damage induced by scopolamine, it is still rarely reported. Thus, the mechanism of action needs to be further explored. This study was to investigate the effect of GSLS on scopolamine (SCOP)-induced memory damage and the underlying mechanism. Male ICR mice were treated with SCOP (3 mg/kg) for 7 days, with or without GSLS (75 and 150 mg/kg) treatment for 14 days. After GSLS treatment, the memory damage induced by SCOP was significantly ameliorated as shown by the improvement of cholinergic function (AChE and ChAT), brain tissue hippocampus morphology (H&E staining), and oxidative stress (MDA, GSH, and NO). Meanwhile, immunohistochemical assay suggested that GSLS increased the expression of brain-derived neurotrophic factor (BDNF) and Tyrosine Kinase receptor B (TrkB). Further mechanism research indicated that GSLS inhibited the Tau hyperphosphorylation and cell apoptosis by regulating the PI3K/AKT pathway and inhibited neuroinflammation by regulating the NF-[Formula: see text]B pathway, thereby exerting a cognitive impairment improvement effect. This work suggested that GSLS could protect against SCOP-induced memory defects possibly through inhibiting oxidative stress, inhibiting neuroinflammation and cell apoptosis.

Araliaceae

10.1142/s0192415x22500458

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The key gluconeogenic gene PCK1 is crucial for virulence of Botrytis cinerea via initiating its conidial germination and host penetration

Environmental Microbiology (2018)

Jian‐Kang Liu Haowu Chang Yue Liu Yu Qin Yu‐Han Ding

Summary The process of initiation of host invasion and survival of some foliar phytopathogenic fungi in the absence of external nutrients on host leaf surfaces remains obscure. Here, we demonstrate that gluconeogenesis plays an important role in the process and nutrient‐starvation adaptation before the pathogen host invasion. Deletion of phosphoenolpyruvate c arboxy k inase gene BcPCK1 in gluconeogenesis in Botrytis cinerea , the causative agent of grey mould, resulted in the failure of the Δ Bcpck1 mutant conidia to germinate on hard and hydrophobic surface and penetrate host cells in the absence of glucose, reduction in conidiation and slow conidium germination in a nutrient‐rich medium. The wild‐type and Δ Bcpck1 conidia germinate similarly in the presence of glucose (higher concentration) as the sole carbon source. Conidial glucose‐content should reach a threshold level to initiate germination and host penetration. Infection structure formation by the mutants displayed a glucose‐dependent fashion, which corresponded to the mutant virulence reduction. Exogenous glucose or complementation of BcPCK1 completely rescued all the developmental and virulence defects of the mutants. Our findings demonstrate that BcPCK1 plays a crucial role in B. cinerea pathogenic growth and virulence, and provide new insights into gluconeogenesis mediating pathogenesis of plant fungal pathogens via initiation of conidial germination and host penetration.

Conidiation

Gluconeogenesis

10.1111/1462-2920.14112

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Citations (33)

Comparison of hair DNA extraction methods from fur products.

Jingji dongwu xuebao (2018)

Qian Cheng Xu LinYa Wei Liu Bai Suying

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