Jianan Chen

Sun Yat-sen University

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Shiwen Mei

Chinese Academy of Medical Sciences & Peking Union Medical College

Qian Liu

Chinese Academy of Medical Sciences & Peking Union Medical College

Fu-Qiang Zhao

Wuhan University

Zhigang Ren

First Affiliated Hospital of Zhengzhou University

Guangying Cui

First Affiliated Hospital of Zhengzhou University

Ranran Sun

First Affiliated Hospital of Zhengzhou University

Haiyu Shen

Changchun University of Science and Technology

Fangze Wei

Chinese Academy of Medical Sciences & Peking Union Medical College

La‐Sheng Long

Xiamen University

Fang Ren

Dalian Medical University

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Sun Yat-sen University

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Peking University

First Affiliated Hospital of Zhengzhou University

Tsinghua University

Huazhong University of Science and Technology

Capital Medical University

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Metagenomic sequencing reveals altered gut microbial compositions and gene functions in patients with non-segmental vitiligo

Research Square (Research Square) (2022)

Mei Luan Mengtian Niu Pengju Yang Dan Han Yudan Zhang

Abstract Background Vitiligo has been correlated with an abnormal gut microbiota. We aimed to systematically identify characteristics of the gut microbial compositions, genetic functions, and potential metabolic features in patients with non-segmental vitiligo. Methods Twenty-five patients with non-segmental vitiligo and 25 matched healthy controls (HCs) were enrolled. Metagenomic sequencing and bioinformatic analysis were performed to determine the gut microbiota profiles. Differences in gut microbiota diversity and composition between patients with vitiligo and HCs were analyzed. Gene functions and gut metabolic modules were predicted with the Kyoto Encyclopedia of Gene and Genomes (KEGG) and MetaCyc databases. Results Compared with HCs, alpha diversity of intestinal microbiome in vitiligo patients was significantly reduced. At the species level, the relative abundance of Staphylococcus thermophiles was decreased, and that of Bacteroides fragilis was increased in patients with vitiligo compared with those of the HCs. Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed representative microbial markers of Lachnospiraceae_bacterium_BX3 , Massilioclostridium_coli , TM7_phylum_sp_oral_taxon_348 and Bacteroides_fragilis for patients with vitiligo. KEGG gene function analysis showed that the NOD-like receptor signaling pathway was significantly enriched in patients with vitiligo. Gut metabolic modules (GMMs) analysis showed that cysteine degradation was significantly down-regulated, and galactose degradation was up-regulated in patients with vitiligo. A panel of 11 microbial features was constructed to distinguish patients with vitiligo from HCs. Conclusions The gut microbial profiles and genetic functions of patients with vitiligo were distinct from those of the HCs. The identified gut microbial markers may potentially be used for earlier diagnosis and treatment targets.

Vitiligo

KEGG

Lachnospiraceae

Dysbiosis

Bacteroides fragilis

10.21203/rs.3.rs-2275067/v1

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The expression and clinical significance of Chymase protein in colorectal cancer tissue

Chin J Colorec Dis (Electronic Edition) (2018)

Jinhua Tao Jian Cui Fan Zhang Jianan Chen Guiyu Wang

Objective To investigate the expression and clinical significance of chymase protein in colorectal cancer tissue. Methods 135 colorectal cancer patients who underwent radical resection were collected at the Cancer Hospital Chinese Academy of Medical Sciences and the Second Affiliated Hospital of Harbin Medical University between September 2010 and September 2012. The expression of Chymase protein in colorectal cancer tissue was detected by immunohistochemistry, and the relationship between Chymase protein and clinicopathological data and prognosis was analyzed. Results The expression of chymase protein in colorectal cancer tissue was lower than that of the adjacent tissues, the difference was of statistically significance (Χ2=4.305, P=0.038). In colorectal cancer tissue, the expression of chymase protein was decreased in distant metastatic patients (P=0.002), the expression of chymase protein was decreased in late N staging (Χ2=54.81, P<0.001), the expression of chymase protein was decreased in Ⅲ+ Ⅳ patients (Χ2=50.84, P<0.001), survival analysis indicated that patients with low expression of chymase protein had poor prognosis (X2=10.501, P=0.001). Conclusion Chymase protein may be a molecular target for prognosis of colorectal cancer. Key words: Colorectal neoplasms; Neoplasm metastasis; Prognosis; Chymase

Chymase

Clinical Significance

10.3877/cma.j.issn.2095-3224.2018.01.008

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Reprogramming of a human induced pluripotent stem cell line from a long QT syndrome patient harboring a heterozygous mutation of c.1537C > T in SCN5A gene

Stem Cell Research (2021)

Jianan Chen Yesang Song Junqing Gu Lian Chen

Long QT syndrome (LQTS) often causes malignant arrhythmia and syncope. Mutations in the SCN5A gene are the main cause of type 3 LQTS. In this study, we generated a human induced pluripotent stem cell line ZJYYPHi001-A from a female LQTS patient harboring a heterozygous mutation in SCN5A using non-integrative Sendai virus. This cell line expressed pluripotency markers, had the normal karyotype and could differentiate into three germ layers in vitro.

Reprogramming

Germ layer

Sendai virus

10.1016/j.scr.2021.102576

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Corrigendum: Differential Expression Analysis Revealing CLCA1 to Be a Prognostic and Diagnostic Biomarker for Colorectal Cancer

Frontiers in Oncology (2020)

Fangze Wei Shiwen Mei Zhijie Wang Jianan Chen Haiyu Shen

CORRECTION article Front. Oncol., 18 December 2020Sec. Gastrointestinal Cancers Volume 10 - 2020 | https://doi.org/10.3389/fonc.2020.634122

10.3389/fonc.2020.634122

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Survey on iodine nutrition and thyroid function among residents in Longyan of Fujian

Chinese Preventive Medicine (2013)

Jianan Chen

Objective To understand the current status of iodine nutritional level and thyroid function among residents in Longyan,and to provide evidence for scientific iodine supplementation.Methods Residents from both urban and rural areas were surveyed.B ultrasound was used to determine the size of thyroid among children.Urinary iodine level and iodine concentration in salt were determined by arsenic cerium catalytic spectrophotometric assay and by direct titrimetric method.Thyroid function parameters were measured using direct chemiluminescence immunoassay.χ2 test and Median test were used for data analysis.Results The median value of Thyroid volume among children aged 8to 10years was 2.54ml with the goiter rate of 1.67%(4/239).There was no significant difference between children from urban and rural areas(P0.05).The coverage rate of iodized salt was 100%(403/403houshold),and qualified iodized salt intake rate was 95.29%(384/ 403).The median values of urinary iodine among children,adults,pregnant and lactating women were 332.3,233.9,162.3and 152.6μg/L,respectively.Children from both areas and adults from rural area showed excessive iodine intake.Abnormal thyroid function rate was 18.52%(115/621).Majority of residents with low FT4level came from urban area(P0.01).However,most of those with subclinical hypothyroidism were from rural area(P0.05).Conclusions To reinforce the monitor of iodine nutritional level and thyroid function and to adjust salt iodization standard are important methods to meet iodine nutritional needs for different population.

Iodised salt

Subclinical infection

Source

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The clinical characteristics, gene mutations and outcomes of myelodysplastic syndromes with diabetes mellitus

Journal of Cancer Research and Clinical Oncology (2024)

Fanhuan Xu Jiacheng Jin Juan Guo Feng Xu Jianan Chen

Abstract Purpose Diabetes mellitus (DM) is the second most common comorbidity in myelodysplastic syndromes (MDS). The purpose of the study was to investigate the clinical characteristics of MDS patients with DM. Methods A retrospective analysis was performed on the clinical data of 890 MDS patients with or without DM. Clinical data, including genetic changes, overall survival (OS), leukemia-free survival (LFS) and infection, were analyzed. Results Among 890 patients, 184 (20.7%) had DM. TET2 and SF3B1 mutations occurred more frequently in the DM group than those in the non-DM group ( p = 0.0092 and p = 0.0004, respectively). Besides, DM was an independent risk factor for infection (HR 2.135 CI 1.451–3.110, p = 0.000) in MDS. Compared to non-DM patients, MDS patients with DM had poor OS and LFS ( p = 0.0002 and p = 0.0017, respectively), especially in the lower-risk group. While in multivariate analysis, DM did not retain its prognostic significance and the prognostic significance of infection was maintained (HR 2.488 CI 1.749–3.538, p = 0.000). Conclusions MDS patients with DM have an inferior prognosis which may due to higher infection incidence, with TET2 and SF3B1 mutations being more frequent in those cases.

Hematology

Clinical Significance

10.1007/s00432-023-05591-4

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Magnetic 3d–4f Chiral Clusters Showing Multimetal Site Magneto-Chiral Dichroism

Journal of the American Chemical Society (2022)

Xing Wang Shi‐Qiang Wang Jianan Chen Jian‐Hua Jia Cheng Wang

Here, we report the molecular self-assembly of hydroxido-bridged {Ln5Ni6} ((Ln3+ = Dy3+, Y3+) metal clusters by the reaction of enantiopure chiral ligands, namely, (R/S)-(2-hydroxy-3-methoxybenzyl)-serine), with NiII and LnIII precursors. Single-crystal diffraction analysis reveals that these compounds are isostructural sandwich-like 3d–4f heterometallic clusters showing helical chirality. Direct current magnetic measurements on {Dy5Ni6} indicates ferromagnetic coupling between DyIII and NiII centers, whereas those on {Y5Ni6} denote that the NiII centers are antiferromagnetically coupled and/or magnetically anisotropic. Magneto-chiral dichroism (MChD) measurements on {Dy5Ni6} and its comparison to that of {Y5Ni6} provide the first experimental observation of intense multimetal site MChD signals in the visible–near-infrared region. Moreover, the comparison of MChD with natural and magnetic circular dichroism spectra unambiguously demonstrate for the first time that the MChD signals associated with the NiII d–d transitions are mostly driven by natural optical activity and those associated with the DyIII f–f transitions are driven by magnetic optical activity.

Isostructural

Enantiopure drug

Chirality

Vibrational Circular Dichroism

10.1021/jacs.2c03049

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Facet joint tropism, pelvic incidence and intervertebral height index: associations with facet joint osteoarthritis in lumbar spinal stenosis

The Spine Journal (2023)

Huihong Shi Shaoguang Li Song Liu Wenjun Hu Jianan Chen

Pelvic tilt

Facet (psychology)

10.1016/j.spinee.2023.10.001

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ADM-Loaded GelMA hydrogel promotes endogenous dental pulp regeneration: an in vitro and in vivo study

Journal of Endodontics (2024)

Yangpeng Zhao Qian Zhang Song Zhang Jianan Chen Lingtong Kong

To prepare a gelatin methacryloyl (GelMA) hydrogel scaffold embedded with adrenomedullin (ADM) and investigate its impact and underlying mechanisms in endogenous pulp regeneration. ADM was evenly distributed within the GelMA hydrogel through a simple and conventional physical mixing technique. The scaffold underwent characterization via scanning electron microscopy, alongside assessments of swelling, degradation, and release properties. Biocompatibility was evaluated using cytoskeletal and live-dead staining techniques. The hydrogel's influence on dental pulp stem cells' proliferation, migration, and differentiation was assessed with CCK-8 assays, Transwell assays, and alizarin red and alkaline phosphatase staining. Transcriptomics provided insights into potential mechanisms. The angiogenic effects on umbilical vein endothelial cells were examined using scratch and tube formation assays. In vivo, the composite hydrogel's regenerative capacity was tested in a rat model of pulp regeneration. Statistical analysis involved Student's t-test and one-way analysis of variance, with significance set at P < .05. The ADM-loaded GelMA (GelMA@ADM) hydrogel displayed a porous architecture under electron microscopy conducive to cell adhesion and demonstrated excellent biocompatibility. In vitro experiments showed that GelMA@ADM significantly boosted dental pulp stem cells' migration, proliferation, and differentiation, and enhanced the angiogenic activity of umbilical vein endothelial cells after one week of treatment. Corresponding in vivo experiments revealed that GelMA@ADM facilitated the formation of new vascularized pulp tissue after 2 weeks of treatment. The GelMA@ADM hydrogel effectively promotes dental pulp stem cells' proliferation and differentiation, augments vascularization by umbilical vein endothelial cells, and fosters the creation of new vascularized pulp tissue. These findings underscore the potential of GelMA@ADM hydrogel for endogenous pulp regeneration.

10.1016/j.joen.2024.11.011

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Effect of Multi-Mode Thermosonication on the Microbial Inhibition and Quality Retention of Strawberry Clear Juice during Storage at Varied Temperatures

Foods (2022)

Min Feng Bimal Chitrakar Jianan Chen Md. Nahidul Islam Benxi Wei

Strawberry juice, which is rich in nutrients and charming flavor, is favored by consumers. To explore whether multi-mode thermosonication (MTS) can ensure the quality stability of strawberry clear juice (SCJ) during storage, the effects of microbial inhibition, enzyme activity, and physicochemical properties of SCJ pretreated by MTS were evaluated during storage at 4, 25, and 37 °C in comparison with thermal pretreatment (TP) at 90 °C for 1 min. The MTS, including dual-frequency energy-gathered ultrasound pretreatment (DEUP) and flat sweep-frequency dispersive ultrasound pretreatment (FSDUP), were conducted at 60 °C for 5 and 15 min, respectively. Results showed that the total phenols, flavonoids, anthocyanins, ascorbic acid, and DPPH free radical scavenging ability of SCJ decreased during the storage period. The control sample of SCJ was able to sage for only 7 days at 4 °C based on the microbiological quality, while the FSDUP and DEUP group extended the storage period up to 21 and 14 days, respectively. The polyphenol oxidase in SCJ pretreated by MTS did not reactivate during the storage period. The MTS remarkably (p < 0.05) reduced the color deterioration, browning degree, and nutrient degradation during the storage period. Moreover, the FSDUP group exhibited the maximum shelf life with a minimum loss of quality, demonstrating that it was the most suitable processing method for obtaining high-quality SCJ. It can be concluded that the MTS has the potential to inhibit enzymatic browning, inactivating microorganisms, preserve original quality attributes, and prolong the shelf life of SCJ.

CRANBERRY JUICE

Retention time

Fruit juice

10.3390/foods11172593

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Citations (14)