Min Zheng

Wenzhou Medical University

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Jun Ge

Tianjin University of Traditional Chinese Medicine

Jiang Li

Sun Yat-sen University

Bi‐Cheng Liu

Zhongda Hospital Southeast University

Dan Liu

First Affiliated Hospital of Xi'an Jiaotong University

Fan Yang

First Affiliated Hospital of Wenzhou Medical University

Lin‐Li Lv

Zhongda Hospital Southeast University

Meiling Huang

Wuhan University

Du-Ping Huang

Wenzhou Medical University

Yuke Tian

University of Electronic Science and Technology of China

Qingkui Guo

Tongren Hospital

Cooperative Institutions

Wenzhou Medical University

First Affiliated Hospital of Wenzhou Medical University

Sichuan University

Chinese Academy of Sciences

University of Electronic Science and Technology of China

Shanghai Jiao Tong University

Nanjing University of Chinese Medicine

Jiangsu Province Hospital

National Institute for Occupational Health and Poison Control

Nanjing University

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Urinary mRNA expression of CCN2/CCN3 as a noninvasive marker for monitoring glomerular structure changes in nondiabetic chronic kidney disease

Biomarkers (2012)

Long Chen Yonggui Wu Dan Liu Lin‐Li Lv Min Zheng

Background: We investigated whether urinary mRNA of connective tissue growth factor (CCN2) and nephroblastoma overexpressed gene (CCN3) can provide clinical insight into the management of patients with nondiabetic CKD.Methods: Urinary mRNA expression of CCN2 and CCN3 were measured by Real-time PCR in 35 CKD patients and 12 controls.Results: Urinary mRNA of CCN2 and CCN3 were distinctively greater in CKDs than healthy controls. Urinary CCN3/CCN2 mRNA ratio correlated to the degree of glomerular histological changes in those who received renal biopsy.Conclusion: Urinary CCN3/CCN2 mRNA ratio may be a useful noninvasive biomarker for evaluating patients with nondiabetic CKD prior to renal biopsy.

Clinical Significance

10.3109/1354750x.2012.722229

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Citations (4)

Genetic landscape of breast cancer and mutation tracking with circulating tumor DNA in Chinese women

Aging (2021)

Yinghao Wang Lizhi Lin Linhong Li Jialiang Wen Yili Chi

Considerable efforts have been devoted to exploring the breast cancer mutational landscape to understand its genetic complexity. However, no studies have yet comprehensively elucidated the molecular characterization of breast tumors in Chinese women. This study aimed to determine the potential clinical utility of peripheral blood assessment for circulating tumor-derived DNA (ctDNA) and comprehensively characterize the female Chinese population's genetic mutational spectrum. We used Omi-Seq to create cancer profiles of 273 patients enrolled at The First Affiliated Hospital of Wenzhou Medical University. The gene landscape results indicate PIK3CA and TP53 as the most frequently detected genes, followed by ERBB2, in Chinese breast cancer patients. The accuracy of ERBB2 copy number variations in tissue/formalin-fixed and paraffin-embedded samples was 95% with 86% sensitivity and 99% specificity. Moreover, mutation numbers varied between different molecular cell-free DNA subtypes, with the basal-like patients harboring a higher number of variants than the luminal patients. Furthermore, ratio changes in the max ctDNA allele fraction highly correlated with clinical response measurements, including cancer relapse and metastasis. Our data demonstrate that ctDNA characterization using the Omi-Seq platform can extend the capacity of personalized clinical cancer management.

10.18632/aging.202888

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The roles of connective tissue growth factor and integrin‐linked kinase in high glucose‐induced phenotypic alterations of podocytes

Journal of Cellular Biochemistry (2011)

Houyong Dai Min Zheng Lin‐Li Lv Ri‐Ning Tang Kun‐Ling Ma

Abstract Emerging evidence has suggested that podocytes undergo epithelial–mesenchymal transition (EMT) in diabetic nephropathy (DN). Connective tissue growth factor (CTGF) and integrin‐linked kinase (ILK) are involved in the progression of DN. However, the underlying mechanisms of EMT are not well understood. The study aimed to investigate the roles of CTGF and ILK in high glucose‐induced phenotypic alterations of podocytes and determine whether ILK signaling is downstream of CTGF. The epithelial marker of nephrin and the mesenchymal marker of desmin were investigated by real‐time RT‐PCR and Western blotting. The results demonstrated that podocytes displayed a spreading, arborized morphology in normal glucose, whereas they had a cobblestone morphology in high glucose conditions, accompanied by decreased nephrin expression and increased desmin expression, suggesting podocytes underwent EMT. In response to high glucose, CTGF and ILK expression in podocytes were increased in a dose‐ and time‐dependent manner, whereas the increase did not occur in the osmotic control. Furthermore, the inhibition of CTGF with anti‐CTGF antibody prevented the phenotypic transition, as demonstrated by the preservation of epithelial morphology, the suppression of high glucose‐induced desmin overexpression and the restoration of nephrin. Of note, the upregulation of ILK induced by high glucose was partially blocked by the inhibition of CTGF. In summary, these findings suggested that CTGF and ILK were involved in high glucose‐induced phenotypic alterations of podocytes. ILK acted as a downstream kinase of CTGF and high glucose‐induced ILK expression might occur through CTGF‐dependent and ‐independent pathways. J. Cell. Biochem. 113: 293–301, 2012. © 2011 Wiley Periodicals, Inc.

Nephrin

Integrin-linked kinase

Desmin

Sorbinil

Sphingosine kinase 1

10.1002/jcb.23355

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Citations (31)

Solving the scalability issue in quantum-based refinement: Q|R#1

Acta Crystallographica Section D Structural Biology (2017)

Min Zheng Nigel W. Moriarty Yanting Xu Jeffrey R. Reimers Pavel V. Afonine

Accurately refining biomacromolecules using a quantum-chemical method is challenging because the cost of a quantum-chemical calculation scales approximately as nm, where n is the number of atoms and m (≥3) is based on the quantum method of choice. This fundamental problem means that quantum-chemical calculations become intractable when the size of the system requires more computational resources than are available. In the development of the software package called Q|R, this issue is referred to as Q|R#1. A divide-and-conquer approach has been developed that fragments the atomic model into small manageable pieces in order to solve Q|R#1. Firstly, the atomic model of a crystal structure is analyzed to detect noncovalent interactions between residues, and the results of the analysis are represented as an interaction graph. Secondly, a graph-clustering algorithm is used to partition the interaction graph into a set of clusters in such a way as to minimize disruption to the noncovalent interaction network. Thirdly, the environment surrounding each individual cluster is analyzed and any residue that is interacting with a particular cluster is assigned to the buffer region of that particular cluster. A fragment is defined as a cluster plus its buffer region. The gradients for all atoms from each of the fragments are computed, and only the gradients from each cluster are combined to create the total gradients. A quantum-based refinement is carried out using the total gradients as chemical restraints. In order to validate this interaction graph-based fragmentation approach in Q|R, the entire atomic model of an amyloid cross-β spine crystal structure (PDB entry 2oNA) was refined.

Quantum chemical

Quantum Chemistry

Non-covalent interactions

Graph partition

10.1107/s2059798317016746

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Citations (25)

TRIO Amplification and Abundant mRNA Expression Is Associated with Invasive Tumor Growth and Rapid Tumor Cell Proliferation in Urinary Bladder Cancer

American Journal Of Pathology (2004)

Min Zheng Ronald Simon Martina Mirlacher Robert Maurer Thomas Gasser

Tissue microarray

Comparative genomic hybridization

Tumor progression

10.1016/s0002-9440(10)63275-0

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Citations (65)

[Changes of pathologic feature and microtubulin associated protein 2 in nervous system of hens with organophosphate-induced delayed neuropathy induced by 2,4,6-trimethylbenzoyl phenylphosphonate].

Zhonghua laodong weisheng zhiyebing zazhi (2010)

Li Liu Guangyun Xie Min Zheng Jian Wang Wen‐Jin Zhao

To develop the organophosphate-induced delayed neuropathy (OPIDN) hen model with 2,4,6-trimethylbenzoyl phenylphosphonate (TOCP), and observe the change of pathology and investigate the alterations of microtubulin associated protein 2 (MAP2).48 adult hens were randomly divided into four groups, including three experimental groups and control group (n = 12 each group). The hens in three experimental groups were treated with TOCP by gavage at single dosages of 250, 500 and 750 mg/kg respectively while the control hens received an equivalent volume of corn oil by gavage. All hens were sacrificed after 21 days of treatment. Half hens in each group were dissected for HE examination and myelin straining of brain, spinal cord and sciatic nerve while brains of another half hens were dissected for the determination of MAP2 by western blotting.The delayed neurotoxicity symptoms of hens both in 500 and 750 mg/kg groups were consistently observed. The pathological changes of brain, spinal cord and sciatic nerve in 500 and 750 mg/kg groups showed nerve cells difference necrosis, increased cytoplasm basophilia, microglia proliferation, mono-nuclear and lymphocyte infiltration, myelin sheath extensive up to part of them disaggregation deletion. Compared with the control group, at 500 and 750 mg/kg respectively the increase of MAP2 was 25% and 23% (P < 0.01 and P < 0.05).The histopathologic changes of OPIDN caused by TOCP have dose-response relationship. The changes of MAP2 in nervous system may contribute to the occurrence and development of TOCP induced delayed neurotoxicity.

Infiltration (HVAC)

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Effects of miR-132-3p on progress and epithelial mesenchymal transition of non-small cell lung cancer via regulating KLF7

Journal of Thoracic Disease (2021)

Ning Wang Ye Xu Qingkui Guo Zhu Chen Wen Zhao

MicroRNAs (miRNAs) often appear as oncogenes or tumor suppressor genes. The aim of this research was to examine miR-132-3p and Kruppel-like factor 7 (KLF7) effects in the development of non-small cell lung cancer (NSCLC).We used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to determine miR-132-3p expression in tissue specimens and 6 cells (A549, H1650, H292, H1299, H1944, BEAS-2b). Luciferase report forecasted the targeting relationship between miR-132-3p and KLF7. The expression of KLF7 and interstitial protein was determined by western blot. Proliferation test and Transwell assay were adopted for examining cell development. The Cell Counting Kit-8 (CCK-8) colorimetric method was used to observe the effects of miR-132-3p and KLF7 on the proliferation, metastasis, and invasion of NSCLC tumor cells. In order to determine whether the metastasis of NSCLC tumor cells was epithelial-mesenchymal transition (EMT)-mediated, supplementary experiments with E-cadherin and vimentin were performed.An increased expression of miR-132-3p was detected in NSCLC. Its mimic promoted the proliferation of tumor cells. As an immediate site of miR-132-3p, KLF7 was reversely adjusted via miR-132-3p and restrained the development of tumor cells in NSCLC, the effects of which were attenuated via KLF7 over-expression. Besides, the presence of EMT-related diversions was confirmed in NSCLC.By targeting KLF7, miR-132-3p was capable of promoting the proceeding of NSCLC tumor cells. We discovered miR-132-3p/KLF7 route may exhibit curative target for NSCLC.

10.21037/jtd-21-353

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Citations (8)

Protective Effect of CpG-Oligodeoxynucleotides Against Low- and High-LET Irradiation

Cellular Physiology and Biochemistry (2014)

Chao Zhang Min Zheng Xiang-hui Zhu Sha Li Ni Jin

Background/Aims: CpG-oligodeoxynucleotides (ODNs) are synthetic DNA sequences containing unmethylated cytosine-guanine motifs with potent immunomodulatory effects. Previous reports showed a powerful protective effect of CpG-ODN against the damage induced by low-LET γ-rays. In this study, we explored whether CpG-ODN also protects against the damage induced by high-LET irradiation. Parallel experiments were performed with low-LET irradiation. Methods: RAW264.7 cells were incubated with 1 μM of CpG-ODN after γ-ray or carbon-beam irradiation. Cell death was then measured by PI/DAPI double staining, cell survival was assessed by colony-formation assays, DNA damage was evaluated by comet assays, cell cycle was monitored by flow cytometry, and the levels of apoptosis-related proteins were detected by western blots. Results: When irradiated cells were treated with the CpG-ODN, cell viability decreased, cell survival increased, DNA damage and G2/M-phase arrest were ameliorated, and apoptosis was inhibited. Conclusions: The CpG-ODN showed protective effects against low-LET γ-ray and high-LET carbon-beam irradiation. These effects might be associated with the repair of DNA damage and inhibition of apoptosis.

DAPI

Comet Assay

CpG site

Viability assay

CpG Oligodeoxynucleotide

10.1159/000366368

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Citations (7)

NFIX Downregulation Independently Predicts Poor Prognosis in Lung Adenocarcinoma, but not in Squamous Cell Carcinoma

Future Oncology (2018)

Jun Ge Hang Dong Ye Yang Bin Liu Min Zheng

Aim: To study the expression profile of NFIX, its prognostic value and the mechanism of its dysregulation in lung adenocarcinoma (LUAD). Patients & materials: A retrospective study was performed by using data from the Cancer Genome Atlas and the Human Protein Atlas. Results: High NFIX RNA expression was an independent prognostic factor of favorable overall survival (HR: 0.687, 95% CI: 0.496–0.951; p = 0.024) and recurrence-free survival (HR: 0.700, 95% CI: 0.493–0.994, p = 0.046) in LUAD, but not in lung squamous cell carcinoma. NFIX DNA hypermethylation was associated with significantly decreased NFIX expression and shorter overall survival and recurrence-free survival in LUAD. Conclusion: NFIX downregulation might independently predict poor prognosis in LUAD. DNA hypermethylation might be an important cause of the downregulation.

10.2217/fon-2018-0164

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Citations (11)

Alteration of gut microbial community after N,N-Dimethylformamide exposure

The Journal of Toxicological Sciences (2017)

Man Zhang Min Zheng Zhijun Wu Mingyue Guan Shuai Liu

N,N-Dimethylformamide (DMF), a solvent commonly used in factories, can induce liver toxicities, including hepatitis, fibrosis, cirrhosis and hepatoma. It is well known that the gut microbial community plays a role in the metabolism of many toxic substance and in liver regeneration. However, the effect of DMF on rat gut microbial community is poorly understood. The gut microbiotas in control rats and rats exposed to DMF were characterized by high-throughput sequencing of the bacterial 16S rRNA gene. The levels of biochemical parameters in the serum of rats, including cholesterol, bile acid, alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were evaluated. The weight was lower in the DMF exposure group than in the control group. DMF exposure led to changes in gut microbiotas that were reflected in a decreased abundance of Prevotellaceae, Lactobacillaceae, and increased abundance of S24-7, Baceroidaceae, Rikenellaceae and Peptostreptococcaceae. Compared with control group, the cholesterol level was substantially reduced in the DMF exposure group (p < 0.05), while the concentration of bile acid was significantly increased in the DMF exposure group (p < 0.05). The present data established that the gut microbiotasy were changed after DMF exposure, and it revealed the relationship between DMF and gut microbiotas for the first time.

Dimethyl formamide

10.2131/jts.42.241

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Citations (13)