Dongsheng He

South China Agricultural University

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i-10 index

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Co-Authors

Junsen Feng

South China Agricultural University

Yongsheng Xie

University of Science and Technology of China

He Yan

South China University of Technology

Yibo Chen

Guangzhou University

Hongbo Yi

Guangdong Academy of Agricultural Sciences

Danping Su

Sichuan University

Li Wang

Zunyi Medical University

Xiaoru Wang

University of Jinan

Weiyou Cai

South China Agricultural University

Liuming Wei

South China Agricultural University

Cooperative Institutions

South China Agricultural University

Key Laboratory of Guangdong Province

Ministry of Agriculture and Rural Affairs

Guangdong Academy of Agricultural Sciences

Chinese Academy of Agricultural Sciences

Zhaoqing University

Shanghai Jiao Tong University

Huazhong Agricultural University

Institute of Animal Science

China Pharmaceutical University

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Near-Complete Genome Sequence of a Newly Emerging Subgenotype of Atypical Porcine Pestivirus

Microbiology Resource Announcements (2020)

Xiaoru Wang Yongsheng Xie Dongsheng He He Yan

This study reports the near-complete genome sequence of GD-CT4, a strain of a newly emerging subgenotype of atypical porcine pestivirus detected in a newborn piglet with congenital tremors in Guangdong Province, China. This sequence will improve the understanding of the epidemiology and genetic characteristics of atypical porcine pestivirus.

Pestivirus

Sequence (biology)

Strain (injury)

10.1128/mra.00115-20

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Citations (3)

Diagnosis and Treatment of Pasteurellosis in Rhizomys sinensis

China Animal Husbandry & Veterinary Medicine (2009)

Dongsheng He

In December 2008,there occurred a serious disease that nearly 100 Rhizomys sinensis died in a group of 200 Rhizomys sinensis in Guangdong province.According to the epidemiological investigation,clinical symptoms,autopsy lesion,morphology,biochemical and mice inoculution test,a Rhizomys sinensis Pasteurella strain was isolated and confirmed.It was demonstrated that the isolate strain was highly sensitive to Penicillin,Norfloxacin,Ofloxacin,Ciprofloxacin,CephalosporinⅥ in drug sensitivity test and resistant to other antibiotics,such as Kanamycin,Gentamicin,Lincomycin.Finally,the outbreak disease has been controlled timely with medication of high-sensitivity.It provided the diagnosis for the Rhizomys sinensis pasteurellosis in clinic.

Pasteurellosis

Lincomycin

Kanamycin

Antibiotic sensitivity

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Genetic variation and phylogenetic analysis of hemagglutinin genes of H9 avian influenza viruses isolated in China during 2010–2012

Veterinary Microbiology (2013)

Ruiai Chen Hanzhang Lai Lin Li Yu-Peng Liu Weilan Pan

Lineage (genetic)

Flock

10.1016/j.vetmic.2013.04.005

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Citations (21)

The Prevalence, Genetic Characterization, and Evolutionary Analysis of Porcine Pegivirus in Guangdong, China

Virologica Sinica (2020)

Yongsheng Xie Xiaoru Wang Junsen Feng Liuming Wei Gen Li

Molecular Epidemiology

Genetic Analysis

10.1007/s12250-020-00240-3

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Nicotinic Acid Upregulates Intestinal Antimicrobial Peptides via Manipulating the Histone Acetylation Modification to Enhance the Resistance of Escherichia Coli Infection in Weaned Piglets

Research Square (Research Square) (2021)

Junsen Feng Dongsheng He Tianbao Chen Weiyou Cai Yibo Chen

Abstract Background: Nicotinic acid (NA) has been used to treat different inflammatory disease with positive influence, the mechanisms by which NA exerts its anti-inflammatory effects remain largely undefined. Here we proposed a new mechanism that NA manipulated endogenous antimicrobial peptides (AMPs) which contributed to the elimination of enterotoxigenic Escherichia coli (ETEC) K88, and thus affects the alleviation of inflammation. Results: The results showed that NA alleviated the clinical symptoms of weaned piglets infected with ETEC K88. NA significantly reduced the amount of ETEC K88 in the spleen and liver ( P < 0.05). The intestinal morphological damage caused by ETEC K88 infection was alleviated by NA in weaned piglets. In addition, NA significantly alleviated the expression of inflammatory cytokine (IL-6, IL-8, and TNF-α) in the serum and intestines of weaned piglets infected with ETEC K88 ( P < 0.05). and increased in intestinal mucosa. NA significantly increased the content of SIgA and the expression of antimicrobial peptides (pBD2, PG1-5 and PR39) in intestines of weaned pigs. NA increased the diversity of microflora in colonic contents, while NA significantly reduced the relative abundance of Bacteroidetes, Bacteroidales and bacteroidia in weaned piglets infected with ETEC K88 ( P < 0.05). Furthermore, the NA group significantly reduced the level of HDAC7 in jejunum ( P < 0.05) and increased the level of SIRT1 in the colon compared with the Control group. Moreover, NA significantly increased the levels phosphorylation of histone H3 at Ser10 (pH3S10) in ileum and the levels of acetylation of lysine 9 on histone 3 (acH3K9) and acH3K27 in colon ( P < 0.05) in weaned piglets infected with ETEC K88 ( P < 0.05). Conclusions: NA could alleviate the clinical symptoms, the damage of intestinal morphology, and intestinal inflammation in weaned piglets infected ETEC K88 through enhancing the endogenous AMPs expression by manipulating the histone acetylation modification.

Enterotoxigenic Escherichia coli

Jejunum

10.21203/rs.3.rs-513837/v1

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Complete Genome Sequence of Senecavirus A Strain SVV HN16 Identified in China

Genome Announcements (2017)

Tianxia Luo Shuaifei Xu Jingfeng Xiong Danping Su Dongsheng He

ABSTRACT A virus that caused blisters and ulcers in pigs in China was detected to be a strain of Senecavirus A (SVA). Complete genome sequencing and analysis results showed that the isolate shares 93.8% to 99.1% identity with other isolates that have been reported, proving that a new isolate of SVA was found in China.

Strain (injury)

10.1128/genomea.01168-17

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Citations (8)

Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of enterocytozoon hepatopenaei (EHP)

Aquaculture Reports (2021)

Gen Li Feng Cong Weiyou Cai Jinhui Li Miaoli Wu

The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes retardation of shrimp growth, leading to significant financial losses in shrimp aquaculture. Therefore, the development of an efficient and sensitive detection method will be conducive to the prevention and control of the shrimp parasite. In this study, we developed and evaluated a rapid real-time recombinase polymerase amplification (RPA) method that can detect EHP within 15 min at a constant temperature of 38.5 °C. The detection limit of this EHP RPA was 10 copies/μL of DNA molecules per reaction. The specificity of EHP RPA was tested, and the assay did not cross-react with white spot syndrome virus (WSSV), shrimp hemocyte iridescent virus (SHIV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), or Vibrio parahaemolyticus. Field and clinical applicability of this assay was evaluated using 61 field samples. The coincidence rate of the detection results for the clinical samples between RPA and qPCR was 95.1 %. In summary, the real-time RPA analysis provides an efficient and sensitive detection method for EHP.

Recombinase Polymerase Amplification

White spot syndrome

10.1016/j.aqrep.2020.100584

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Citations (10)

Development of Haemophilus parasuis Attenuated Strains

China Animal Husbandry & Veterinary Medicine (2012)

Dongsheng He

According to Haemophilus parasuis which was isolated from the department,the strain of poison strong,stable growth and virulence stability was selected as the original strain.The original strain was induced to cause a mutation by chemical agents,then after toxicity test,significantly less virulent strains were selected.The safety trials found that these attenuated strains had a high security,and layed the foundation for the development of attenuated vaccine.

Strain (injury)

Attenuated vaccine

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Haemophilus parasuis Strains Prevalent in Guangdong Isolated and Identified with the Genetic Analysis

China Animal Husbandry & Veterinary Medicine (2011)

Dongsheng He

This study from different areas of Guangdong province inspection disease material success in the identification of four strains Haemophilus parasuis and according to Haemophilus parasuis 16S rRNA specificity gene detection of Hps by PCR and genetic gene sequencing allogenic analysis,through which GenBank online than analysis,with domestic and international strains isolated strains 16S rRNA sequence homology in 98.2% above,separation between homology strains in between 99.6% and 100%,which demonstrates that the outbreak strain is pair of Haemophilus parasuis.

Homology

Genetic Analysis

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Sequencing of HN gene of three newcastle disease virus isolates from south China and phylogenic analysis

Zhifeng Qin Dongsheng He Wu HongZhuan Lou Hua Dewei Yang

The hemagglutinin-neuraminidase (HN) is a major pathogenic and immunogenic protein of NDV. One step RT-PCR was employed to amplify a cDNA fraction (including for four of the five hemagglutinin determinants) of HN gene of NDV-WS1, NDV-FS2 and NDV-HY strains using a pair of specific primers. The products were directly sequenced and cloned into the T-T window of pGET-Easy vector. Sequencing revealed that the HN gene fraction of all the strains contained 896 nucleotides, coding for 298 amino acids. Comparison of the nucleotide sequence and deduced amino acid sequence of HN of the isolates showed 89%-99%, 84%-96% and 84%-96% homology respectively with other representative NDV strains; and 90%-98%, 87%-91% and 88%-93% homology for the deduced amino acid sequence. A phylogenetic tree was constructed, and the reuslt indicated that there existed great discrepancy between NDV isolates from South China and II-V genotype in Western countries, but NDV strains from South China were of the same lineage as those from Taiwan, belonging to the novel genotype VII.

Newcastle Disease

Homology

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