Changsheng Zhang

Institute of Oceanology

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Yiguang Zhu

Institute of Oceanology

105

Qingbo Zhang

Chinese Academy of Sciences

Wenjun Zhang

South China Sea Institute Of Oceanology

Haibo Zhang

South China Sea Institute Of Oceanology

Liping Zhang

Chinese Academy of Sciences

Guangtao Zhang

Shanghai University of Traditional Chinese Medicine

Qingbo Zhang

Jiangsu University of Science and Technology

Sumei Li

Institute of Botany

Chunfang Yang

South China Sea Institute Of Oceanology

Jianhua Ju

South China Sea Institute Of Oceanology

Cooperative Institutions

Chinese Academy of Sciences

352

University of California, San Diego

244

Manufacturing Advocacy & Growth Network (United States)

197

University of Chinese Academy of Sciences

171

University of Florida

154

Columbus Oncology and Hematology Associates

140

Peking University

104

Institute of Oceanology

South China Sea Institute Of Oceanology

Digital Video (Italy)

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The Journal of Clinical Pharmacology (2016)

Lisa H. Lam Rosene D. Pirrello Joseph Ma Dennis M. Fisher William Kramer

Aims and Scope: For more than 50 years, clinical pharmacologists, clinical and pharmaceutical researchers, drug development specialists, physicians, nurses, and other medical professionals have relied on The Journal of Clinical Pharmacology (JCP) for original research, special reviews, commentaries, and case reports on all phases of drug development from absorption, disposition, metabolism, excretion interactions, and preferred uses through post-marketing evaluations.

10.1002/jcph.777

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Citations (0)

Effects of elevated CO 2 and drought on the microbial biomass and enzymatic activities in the rhizospheres of two grass species in Chinese loess soil

Geoderma (2016)

Sha Xue Xiaomei Yang Guobin Liu Lingtong Gai Changsheng Zhang

Soil respiration

Medicago sativa

10.1016/j.geoderma.2016.10.025

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Citations (50)

Table of Contents

2020 IEEE International Conference on Plasma Science (ICOPS) (2023)

Eun Kyung Choi N. Riga D. Shiffler K Jensen John Petillo

Table (database)

10.1109/icops45740.2023.10481047

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Chemical Constituents ofDracocephalum forrestii

Planta Medica (2009)

Sumei Li Xian‐Wen Yang Yong‐Li Li Yun‐Heng Shen Lin Feng

A systematic phytochemical examination of the whole plant Dracocephalum forrestii led to the isolation of 4 new and 65 known chemical constituents. By detailed 1D and 2D NMR spectroscopic analyses, the new compounds were identified as 4-hydroxy-3-methoxyphenylethanol 8- O-[(6- O-syringoyl)- beta- D-glucopyranoside] (1), 3,4,5-trimethoxyphenylethanol beta- D-glucopyranoside ( 2), 4- O-[ beta- D-glucopyranosyl-(1 --> 3)- alpha- L-rhamnopyranosyl]phenylethylcinnamamide (3), and 9''- O- N-butyl lithospermate (4). The new isolates were evaluated for inhibitory activities against LPS-induced NO production in RAW 264.7 macrophages. Compound 2 revealed a moderate effect without any cytotoxicity under the assayed concentrations.

Phytochemical

10.1055/s-0029-1185868

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Citations (27)

Discovery and Biosynthesis of Neoenterocins Indicate a Skeleton Rearrangement of Enterocin

Organic Letters (2019)

Liujuan Zheng Xiaodong Jiang Qingbo Zhang Yiguang Zhu Haibo Zhang

Two polyketides neoenterocins A (1) and B (2), featuring a neighboring dicarbonyl motif and a furan-containing 5/6 ring system, were isolated from the enterocin producer Streptomyces sp. SCSIO 11863. Heterologous expression, gene disruptions, and isotope feeding experiments indicated that 1 and 2 were derived from the enterocin biosynthetic gene cluster. However, 2 was demonstrated as an artifact from enterocin via a unique skeleton rearrangement.

Gene cluster

Heterologous expression

Heterologous

10.1021/acs.orglett.9b03460

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Citations (14)

Neutron diffractometer RSND for residual stress analysis at CAEP

Nuclear Instruments and Methods in Physics Research Section A Accelerators Spectrometers Detectors and Associated Equipment (2015)

Jian Li Hong Wang Guangai Sun Bo Chen Chen Yanzhou

Diffractometer

10.1016/j.nima.2015.02.026

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Citations (44)

Characterization of the Amicetin Biosynthesis Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation

Applied and Environmental Microbiology (2012)

Gaiyun Zhang Haibo Zhang Sumei Li Xiao Ji Guangtao Zhang

Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an α-(1→4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthesis gene cluster was cloned from Streptomyces vinaceusdrappus NRRL 2363 and localized on a 37-kb contiguous DNA region. Heterologous expression of the amicetin biosynthesis gene cluster in Streptomyces lividans TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of the amicetin skeleton by the linkage of cytosine, p-aminobenzoic acid (PABA), and the terminal (+)-α-methylserine moieties. The inactivation of the benzoate coenzyme A (benzoate-CoA) ligase gene amiL and the N-acetyltransferase gene amiF led to two mutants that accumulated the same two compounds, cytosamine and 4-acetamido-3-hydroxybenzoic acid. These data indicated that AmiF functioned as an amide synthethase to link cytosine and PABA. The inactivation of amiR, encoding an acyl-CoA-acyl carrier protein transacylase, resulted in the production of plicacetin and norplicacetin, indicating AmiR to be responsible for attachment of the terminal methylserine moiety to form another amide bond. These findings implicated two alternative strategies for amide bond formation in amicetin biosynthesis.

Gene cluster

Disaccharide

10.1128/aem.07185-11

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Citations (43)

Characterization of the flavoenzyme XiaK as an N-hydroxylase and implications in indolosesquiterpene diversification

Chemical Science (2017)

Qingbo Zhang Huixian Li Lu Yu Yu Sun Yiguang Zhu

Flavoenzymes are ubiquitous in biological systems and catalyze a diverse range of chemical transformations. The flavoenzyme XiaK from the biosynthetic pathway of the indolosesquiterpene xiamycin A is demonstrated to mediate the in vivo biotransformation of xiamycin A into multiple products, including a chlorinated adduct as well as dimers characterized by C-N and N-N linkages that are hypothesized to form via radical-based mechanisms. Isolation and characterization of XiaK in vitro shows that it acts as a flavin-dependent N-hydroxylase that catalyzes the hydroxylation of xiamycin A at the carbazole nitrogen to form N-hydroxyxiamycin, a product which was overlooked in earlier in vivo experiments because its chemical and chromatographic properties are similar to those of oxiamycin. N-Hydroxyxiamycin is shown to be unstable under aerobic conditions, and characterization by electron paramagnetic resonance spectroscopy demonstrates formation of an N-hydroxycarbazole radical adduct. This radical species is proposed to serve as a key intermediate leading to the formation of the multiple xiamycin A adducts. This study suggests that non-enzyme catalyzed reactions may play a greater role in the biosynthesis of natural products than has been previously recognized.

Hydroxylation

Biotransformation

Carbazole

10.1039/c7sc01182b

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Citations (41)

Elucidating the Cyclization Cascades in Xiamycin Biosynthesis by Substrate Synthesis and Enzyme Characterizations

Organic Letters (2014)

Huixian Li Yu Sun Qingbo Zhang Yiguang Zhu Shu‐Ming Li

Indolosesquiterpene xiamycin A features a pentacyclic core structure. The chemical synthesis of two key precursors, 3-farnesylindole and 3-(epoxyfarnesyl)-indole, allowed elucidation of the enzymatic cascades forming the pentacyclic ring system of xiamycin A by XiaO-catalyzed epoxidation and the membrane protein XiaH-catalyzed terpene cyclization. The substrate flexibility of XiaI, an indole oxygenase for assembly of the central ring, was also demonstrated.

Terpene

10.1021/ol503399b

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Citations (38)

Nocardiamides A and B, Two Cyclohexapeptides from the Marine-Derived Actinomycete Nocardiopsis sp. CNX037

Journal of Natural Products (2013)

Zhengchao Wu Sumei Li Sang‐Jip Nam Zhong Liu Changsheng Zhang

Two new cyclic hexapeptides, nocardiamides A (1) and B (2), were isolated from the culture broth of marine-derived actinomycete CNX037 strain that was identified as a Nocardiopsis species. The planar structures of nocardiamides A (1) and B (2) were assigned on the basis of 1D and 2D NMR and HRESIMS spectroscopic analyses. Their absolute configurations were deduced by the advanced Marfey's method and chiral-phase HPLC analysis. The challenge of locating two d- and one l-valine residue in 1 and 2 was accomplished by total synthesis using solid-phase peptide synthetic methods. Both 1 and 2 showed negligible antimicrobial activities against seven indicator strains and exhibited no cytotoxicity against HCT-116.

Residue (chemistry)

Absolute Configuration

Strain (injury)

10.1021/np400009a

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Citations (35)