Abstract Background Colorectal cancer remains an incurable disease despite progress in treatment over the past few years. Methods In this report, we presented a case on colon cancer leading to the development (metastasis) of colorectal cancer in a 56-year-old woman. Preoperative colonoscopy was performed, and diagnosis revealed a malignant tumor of the colon with a tumor of the abdominal wall, a secondary malignant tumor of the abdominal cavity, a malignant tumor of the ovary. The biopsies revealed a metastatic or invasive grade I-II adenocarcinoma. Laparoscopic was performed after few days. Results During the surgery a metastasis of about 3 cm was found and the metastasis of the pelvic cavity showed that the anterior wall of the rectum and the cervical stump have recurrence of about 4 cm and some small intestinal cancerous adhesions were present in the lesion. Afterwards, the biopsy specimens were reanalyzed, and immunohistological analysis shown this as cancerous tissue. Conclusion When colorectal cancer is detected in a patient with a history of colon cancer and bilateral metastatic ovarian adenocarcinoma, the physician should be very observant of the condition as there is the possibility of recidivism due to poor prognosis. Surgery is the only curative treatment for metastatic colorectal cancer.
Activating transcription factor 4 (ATF4) is a DNA-binding protein widely generated in mammals, which has two biological characteristics that bind the cAMP response element (CRE). The mechanism of ATF4 as a transcription factor in gastric cancer affecting the Hedgehog pathway remains unclear. Here, we observed that ATF4 was markedly upregulated in gastric cancer (GC) using immunohistochemistry and Western blotting assays in 80 paraffin-embedded GC samples and 4 fresh samples and para-cancerous tissues. ATF4 knockdown using lentiviral vectors strongly inhibited the proliferation and invasion of GC cells. ATF4 upregulation using lentiviral vectors promoted the proliferation and invasion of GC cells. We predicted that the transcription factor ATF4 is bound to the SHH promoter via the JASPA database. Transcription factor ATF4 is bound to the promoter region of SHH to activate the Sonic Hedgehog pathway. Mechanistically, rescue assays showed that ATF4 regulated gastric cancer cells' proliferation and invasive ability through SHH. Similarly, ATF4 enhanced the tumor formation of GC cells in a xenograft model.
Objective: To assess whether applying betamethasone-gel to an endotracheal tube is more effective than using an unlubricated endotracheal tube in minimizing the occurrence of postoperative sore throat, hoarseness, and cough. Methods: 144 patients of the American Society of anaesthesiologists-physical status (ASA-PS) I and II, aged 18–65 years of either gender planned for elective surgery under general anaesthesia with endotracheal intubation were included. Patients undergoing ENT /oral cavity / oropharyngeal surgeries and upper respiratory tract infections were excluded. Patients were divided into two groups. In group X, patients were intubated with an endotracheal tube lubricated with 0.05% betamethasone-gel. In group Y patients were intubated with the unlubricated endotracheal tube. All patients were evaluated for the frequency of POST, HOV and PEC at 24 h after extubation. Results: Post-operative sore throat was present in 25 (34.7%) patients in group X and 29 (40.3%) patients in group Y. Post-extubation cough was present in 06 (8.3%) patients in group X and 9 (12.5%) patients in group Y. Hoarseness of voice was present in 04 (5.6%) patients in group X and 6 (8.3%) patients in group Y. A comparison of both groups showed a p-value of 0.0001 which is statistically significant. Conclusion: Using betamethasone-gel to lubricate the endotracheal tube markedly diminishes the frequency and intensity of postoperative airway symptoms. Keywords: Endotracheal intubation, betamethasone, sore throat, cough, hoarseness, anesthesia.
Previously, we have reported cloning of human epidermal growth factor gene from Huh-7 cells and its extracellular expression in Pichia pastoris. The presented work is a detailed report regarding molecular characterization of Huh-7 cells-derived hEGF expressed in Pichia pastoris with special reference to its glycosylation profiling and bioactivity studies. Densitometric scanning of SDS-PAGE separated extracellular proteins from hEGF recombinant Pichia pastoris strain indicated that about 84% of the extracellular proteins were glycosylated. Size exclusion chromatography using Superdex 75 prep grade column was successfully utilized to separate fractions containing glycosylated and non-glycosylated extracellular proteins. In dot blot assay, hEGF was detected in both glycosylated and non-glycosylated fractions. Bioactivity assays revealed that both glycosylated and non-glycosylated fractions were bioactive as determined by cell viability assay. It was also observed that hEGF present in non-glycosylated fraction was relatively more bioactive than hEGF present in glycosylated fraction.
A research has been conducted to study the effect of some concentrations of Noni (Morindacitrifolia L.) fruit extract in controlling anthracnose disease, caused by Colletorichum capsici on thepost-harvested red chilli fruits. The research has been conducted experimentally using a CompletelyRandomized Design consisting of 5 treatments and 4 replications. The treatments are someconcentrations of noni fruit extract : 0%=Mo, 5%=M1,10%=M2,15%=M3 and 20%=M4. Result of theresearch indicated that the concentration of noni fruit extract gave a significantly different effect incontrolling anthracnose disease on post-harvested red chilli fruits. The concentration of noni fruit extractat 20% showed a better effect in controlling anthracnose disease on post-harvested red chilli fruits whichresulted in a smaller in-vitro colony growth of the causal fungal pathogen, a longer incubation period ofthe disease and a lower disease intensity.
With increasing travel and migration, rates of parasitic lung and pleural diseases are increasing in the immunecompetent population in developed countries as well as among immune compromised patients.Amoebic pleuropulmonary disease is the most common complication of amoebic liver abscess, occurring in patients with amoebic liver disease and those with amoebic dysentery.The main source of transmission is the chronically infected human.Stools infected with the cyst form of the parasite may contaminate fresh food or water.The most common symptoms amoebic pleuropulmonary diseases include pain, cough, hemoptysis, and dyspnea.The pain may be pleuritic or localized to the right upperquadrant.The diagnosis of pleuropulmonary amoebiasis may be supported by the clinical manifestation.The diagnosis of pleuropulmonary amoebiasis may be supported by the clinical manifestation of the disease.The paper reviews the epidemiology, transmission and management of pleuropulmonary amoebiasis.
The objective of this research is to study the effect of some concentrations of neem leaves extractin controlling anthracnose disease on post-harvested red chilli fruits. A research has been conductedexperimentally using a Completely Randomized Design, consisting of 6 treatments and 5 replications. Thetreatments are 6 concentrations of neem leaves extract : 0%=M0, 1%=M1, 5%=M2, 10%=M3, 15%= M4and 20%=M. Result of the research indicated that the concentration of neem leaves extract gave differenteffect in controlling anthracnose disease of the red chilli fruits. The concentration of neem leaves extractat 15% and 20% gave a better effect in controlling anthracnose disease of post-harvested red chilli fruits,which resulted in a smaller diameter of in-vitro fungi colony growth, a longer incubation period of thedisease and a lower incidence of the disease.
Stunting merupakan kondisi gizi buruk pada balita usia 24-59 bulan dengan kondisi postur tubuh tidak seusai dengan umur anak. Berbagai faktor yang menyebabkan faktor seperti kondisi ekonomi keluarga dan pengetahuan keluarga. Untuk mengendalikan terjadinya stunting ini diperlukan identifikasi pengetahuan orang tua mengenai pencegahan stunting. Penelitian ini bertujuan untuk mengetahui gambaran pengetahuan orang tua mengenai pencegahan stunting. Metode dalam penelitian ini adalah survei dengan pendekatan deksriptif, teknik pengambilan sampel adalah random dengan pendekatan purposive sampling. Kuesioner ini terdiri dari 20 pertanyaan mengenai pengetahuan umum mengenai stunting, penyebab dan upaya mencegah terjadinya stunting. Responden merupakan orang tua yang memiliki balita. Penelitian ini dilakukan di desa Malinau Hulu kabupaten Malinau. Hasil penelitian ini melibatkan 20 responden yang merupakan orang tua balita dengan status 75% sebagai ibu dan 25% sebagai ayah. Pengetahuan orang tua dengan kategori baik sebanyak 40%, cukup 35% dan kurang 25%. Berdasarkan hasil penelitian ini menunjukkan bahwa perlu upaya untuk meningkatkan pengetahuan orang tua untuk pencegahan stunting.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)