The insect specimens were collected from in four artificial forests of Heshan Low subtropical hilly land in 2001～2002.They are belonged to 14 orders,161 famalies,488 species.In this paper,3 orders,77 families,272 species were recorded.
Additional file 1: Table S1. OTU table of the amplicon sequencing result of the ZymoBIOMICS™ Microbial Community DNA Standard. Table S2. OTU table of the customized mock community with four bacteria. Table S3. OTU table of the customized mock community with seven bacteria. Table S4. Summary of the number of reads and clustered OTUs of samples from the three typical ecosystems. Table S5. Correlation test of the relative abundance of OTUs and physiochemical properties of cow rumen fluid. Table S6. Mapping results of the cloned sequences of specifically designed primers for Nocardia coeliaca to the entire OTU representative sequences.
Objective To construct the eukaryotic expression vector pCDNA3.1(+) containing WT1y gene an assay the specific CTL responses to DNA vaccine of Wilms' tumor gene(WT1y) product.MethodsThe WT1y gene of 321 bp,including HLA-A*2402 anchoring residue,was synthetized,and then transfected into eukaryotic vector calledpCDNA3.1(+).The constructed eukaryotic vector calledpCDNA3.1(+)/WT1y was used to immunize Balb/c mice.WT1-specific antibody was detected in serum of mice by ELISA method.T lymphocyte proliferation was detected by FCM.The CTLs response was detected by co-culture the murine spleen cells and the tumor cells.ResultsRecombinant gene was confirmed using DNA sequencing.WT1-specific antibodies were detected in serum of immunized mice by ELISA.T lymphocyte proliferation of immunized mice was superior to that of control group(P0.01).The CTLs exerted specific lysis against WT1-expressing cells.ConclusionThe DNA vaccine of WT1y can induce specific CTLs,which offers a new way for treatment of Wilms' tumor.
Understanding spatiotemporal dynamics of cotton cultivation patterns is an essential input to cotton farming management, production prediction and policy formulations. Thus, this study demonstrates cotton cultivation dynamics by constructing their change trajectories using multi-temporal Landsat and Sentinel-2 images. Then we reclassified the trajectories into four change trends, namely permanent cotton-field, cotton-field gain, cotton-field loss and unstable changes type. The results showed that increased cotton-cultivated areas were greater than the decreased areas. Cotton fields' expansion gradually appeared in the outer parts of the permanent cotton fields, especially towards the northern mountain front alluvial-plain. Production decline caused by cotton cultivation loss was 3.09 × 108 kg. The observed cotton cultivation dynamics were associated to farmers' options driven by crop price fluctuations, agricultural policies and natural conditions. The production loss was associated to cotton field loss and fields that underwent unstable changes. Management strategies should thus focus intercepting the loss and the unstable change trajectories.
Abstract Endosomal sorting complex required transport (ESCRT) III proteins are essential for membrane remodeling and repair across all domains of life. Eukaryotic ESCRT-III and the cyanobacterial homologs PspA and Vipp1/Imm30 remodel membrane into vesicles, rings, filaments and tubular rods structures. Here our microscopy analysis showed that multicellular bacteria, referred to as magnetoglobules, possess multiple compartments including magnetosome organelles, polyphosphate granules, vesicles, rings, tubular rods, filaments and MVB-like structures. Therefore, membrane remodeling protein PspA might be required for the formation of these compartments, and contribute to the morphogenesis and evolution of multicellularity. To assess these hypotheses, we sequenced nine genomes of magnetoglobules and found a significant genome expansion compared to unicellular magnetotactic bacteria. Moreover, PspA was ubiquitous in magnetoglobules and formed a distinct clade on the tree of eubacterial and archaeal ESCRT-III. The phylogenetic feature suggested the evolution of magnetoglobules from a unicellular ancestor of deltaproteobacterium. Hetero-expression of ellipsoidal magnetoglobule pspA2 gene alone in Escherichia coli resulted in intracellular membrane aggregation. GFP fusion labeling revealed polar location of PspA2 in rod-shaped unicells and regular interval location in filamentous cells. Cryo-electron tomography analysis showed filament bundle, membrane sacculus, vesicles and MVB-like structure in the cells expressing PspA2. Moreover, electron-dense area with a similar distribution as GFP-PspA2 foci in filamentous cells changed the inward orientation of the septum, which might interfere with the cell division. Collectively, these results show the membrane remodeling function of magnetoglobule PspA proteins, which may contribute to morphogenesis and the evolution of multicellularity of magnetotactic bacteria.
According to exon 5 sequences published by GenBank of 2,4-dienoyl-CoA reductase 1(DECR1)gene of bovine(NP_001068891),one pair primer was designed to amplifiy exon 5 of DECR1 gene by using PCR technique for three sheep populations including of German MuttanMerino sheep,Dorper sheep and Texel sheep,the single nucleotide polymorphism of three sheep populations were detected by PCR-SSCP,and compared and analyzed with other species of chicken,rat,sheep,pig,human,bovine,rabbit,dog from GenBank.The results showed that the size of DECR1gene exon 5 was 137 bp,SNPs was not found in exon 5 of DECR1 gene by PCR-SSCP.By means of sequence homology aligment between sequence of exon 5 of sheep DECR1gene and others species,the results showed that homology of different species DECR1gene was 81.02%to 97.08%,the highest similarity was 97.08% between sheep and bovine.The molecular phylogenetic tree constructed by DECR1 gene exon 5sequences showed that sheep clustered with bovine firstly,and secondly with rabbit and dog,and then human and pig.This results indicated that sheep had the most nearest relative relationship with bovine,but the far-thest with chicken.It was consistent with the orthodox taxonomic results,and DECR1 gene was very conservative in the progress of animal evolu-tion.
As the population ages with fewer children, depression symptoms are increasing among the elderly who lack companionship. Intergenerational support is closely related to depression in the elderly; hence how the behavioral patterns and emotional quality of intergenerational support affect depressive symptoms in the elderly should be further explored.To study the effects of intergenerational exchange patterns and intergenerational relationship quality on depressive symptoms in the elderly.A total of 8,015 people over 60 years old in CHARLS in 2018 were selected as the object of this study. First, the correlation between demographics, economic conditions, health status, intergenerational support patterns, intergenerational relationship quality, and depressive symptoms in the elderly were analyzed. Three regression analysis models were established to analyze the relationship between control variables, intergenerational support patterns, intergenerational relationship quality, and depressive symptoms in the elderly. Results: Among the intergenerational economic, care, and emotional exchange modes, the risk of depressive symptoms in the elderly in the mutual support group was 31.8, 38.4, and 25.5% lower than that in the non-communication group. Compared with the elderly with very poor intergenerational relationship quality, the elderly with good, very good, and excellent intergenerational relationship quality had 74.5, 84.0, and 85.6% lower risk of depressive symptoms.Different cultural backgrounds also affect intergenerational exchange patterns and depression in the elderly. During the study of depressive symptoms, two aspects relating to intergenerational support should be considered behaviorally and emotionally: the intergenerational exchange model and the intergenerational relationship quality. As depression in the elderly is affected by multiple factors, the participation and joint efforts of the whole society are required to reduce depressive symptoms in the elderly and realize active aging.The intergenerational exchange pattern of mutual support and the higher quality of the intergenerational relationship can significantly reduce the depressive symptoms of the elderly.
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