Lu Song

University of California, Los Angeles

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Imir G. Metushi

University of California, Los Angeles

Randy Schekman

Howard Hughes Medical Institute

K. H. Brian Lam

University of California, Los Angeles

Xinran Tian

Tianjin University

Vincent Buggs

University of California, Los Angeles

Yan Wang

Qingdao Eighth People's Hospital

Mei Yuan

University of Science and Technology of China

Xiaojie Li

Wuhan Hankou Hospital

Naihe Jing

Guangzhou Institutes of Biomedicine and Health

Gopal Vithlani

University of California, Los Angeles

Cooperative Institutions

Tsinghua University

University of California, Los Angeles

University of California, San Diego

UC San Diego Health System

Shenzhen University

University of California, Berkeley

Chinese Academy of Sciences

University of Chinese Academy of Sciences

University of California System

First Affiliated Hospital of Zhengzhou University

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Extracellular vesicles from neuronal cells promote neural induction of mESCs through cyclinD1

bioRxiv (Cold Spring Harbor Laboratory) (2021)

Lu Song Xinran Tian Randy Schekman

Abstract Extracellular vesicles (EVs) that are thought to mediate the transport of proteins and RNAs involved in intercellular communication. Here, we show dynamic changes in the buoyant density and abundance of extracellular vesicles that are secreted by PC12 cells stimulated with nerve growth factor (NGF), N2A cells treated with retinoic acid to induce neural differentiation and mESCs differentiated into neuronal cells. EVs secreted from in vitro differentiated cells promote neural induction of mouse embryonic stem cells (mESCs). Cyclin D1 enriched within the EVs derived from differentiated neuronal cells contributes to this induction. EVs purified from cells overexpressing cyclin D1 are more potent in neural induction of mESC cells. Depletion of cyclin D1 from the EVs reduced the neural induction effect. Our results suggest that extracellular vesicles regulate neural development through sorting of cyclin D1. Summary Extracellular vesicles (EVs) may mediate intercellular communication through the transfer of cargo molecules. Here, we report that cyclinD1 is sorted into EVs during neuronal differentiation and that these EVs help to promote the neural induction of mouse embryonic stem cells.

Neural Development

10.1101/2021.05.09.443321

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Citations (1)

Materials Selection, Processing, and Characterization Technologies

Jing Ma Lu Song Chen Liu Chengzhou Xin

Magnetoelectric (ME) composites have recently attracted an ever-increasing interest and provoked a great number of research activities, driven by profound physics from coupling between ferroelectric and magnetic orders, as well as potential applications in novel multifunctional devices, such as sensors, transducers, memories, and spintronics. This chapter discusses the development and the consideration of materials selection of polymer-based magnetoelectric (ME) composites, followed by an introduction to the characterization technologies of ferroelectric/piezoelectric, magnetism, and direct/converse ME coupling, which are essential in the study of ME composites. In polymer-based ME composites in which the polymer acts as the binder, two categories are common. One is the structure - magnetostrictive and piezoelectric particles are mixed or separately embedded in a polymer matrix - and the other is that the polymer only acts as the binding boundary layer. The chapter discusses the popular characterization techniques and facilities for magnetism measurement and an important branch, particularly for ME composites, magnetostriction measurement.

Characterization

Magnetism

Converse

Ferroelectric Polymers

Material selection

Smart material

10.1002/9783527801336.ch2

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Discovery of 1-(3-aryl-4-chlorophenyl)-3-( p -aryl)urea derivatives against breast cancer by inhibiting PI3K/Akt/mTOR and Hedgehog signalings

European Journal of Medicinal Chemistry (2017)

Wenlu Li Qinsheng Sun Lu Song Chunmei Gao Feng Liu

GLI1

10.1016/j.ejmech.2017.09.002

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Citations (17)

Connectedness of commodity, exchange rate and categorical economic policy uncertainties — Evidence from China

The North American Journal of Economics and Finance (2022)

Lu Song Gengyu Tian Yonghong Jiang

Spillover effect

Dominance (genetics)

Social Connectedness

10.1016/j.najef.2022.101656

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Citations (33)

Wholemount in situ Hybridization for Spatial-temporal Visualization of Gene Expression in Early Post-implantation Mouse Embryos

BIO-PROTOCOL (2021)

Xianfa Yang Yingying Chen Lu Song Ting Zhang Naihe Jing

Regionalized distribution of genes plays crucial roles in the formation of the spatial pattern in tissues and embryos during development. In situ hybridization has been one of the most widely used methods to screen, identify, and validate the spatial distribution of genes in tissues and embryos, due to its relative simplicity and low cost. However, acquisition of high-quality hybridization signals remains a challenge while maintaining good tissue morphology, especially for small tissues such as early post-implantation mouse embryos. In this protocol, we present a detailed RNA in situ hybridization protocol suitable for wholemount early post-implantation mouse embryos and other small tissue samples. This protocol uses digoxigenin (DIG) labeled riboprobes to hybridize with target transcripts, alkaline phosphatase-conjugated anti-DIG antibodies to recognize DIG-labeled nucleotides, and nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl-phosphate (BCIP) chromogenic substrates for color development. Specific steps and notes on riboprobe preparation, embryo collection, probe hybridization, and color development are all included in the following protocol. Graphic abstract: Overview of Wholemount in situ Hybridization in Early Mouse Embryos.

Riboprobe

Dig

Digoxigenin

10.21769/bioprotoc.4229

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Silencing of microRNA-210 inhibits the progression of liver cancer and hepatitis B virus-associated liver cancer via targeting EGR3

BMC Medical Genetics (2020)

Xiaojie Li Mei Yuan Lu Song Yan Wang

This study was aimed to investigate the regulatory role of microRNA-210 (miRNA-210) on the progression of liver cancer and Hepatitis B virus (HBV)-associated liver cancer.The expression of miRNA-210 was detected in liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells by qRT-PCR. MiRNA-210 was silenced in HepG2 and HepG2.2.15 cells by the transfection of miRNA-210 inhibitor. The cell viability and apoptosis was detected by MTT assay and Annexin V-fluorescein isothiocyanate/propidium iodide staining, respectively. The protein expression of EGR3 was detected by Western blot. The regulatory relationship between EGR3 and miRNA-210 was predicted by TargetScan and identified by Dual luciferase reporter gene assay.MiRNA-210 was overexpressed in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells (P < 0.05). Silencing of miRNA-210 inhibited the viability and promoted the apoptosis of HepG2 and HepG2.2.15 cells (P < 0.05). EGR3 was a target of miRNA-210, which was down-regulated in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells (P < 0.05). Silencing of miRNA-210 increased the mRNA and protein expression of EGR3 (P < 0.05). Silencing of EGR3 reversed the anti-tumor effect of miRNA-210 inhibitor on HepG2 and HepG2.2.15 cells (P < 0.05).Silencing of miRNA-210 inhibits the progression of liver cancer and HBV-associated liver cancer via up-regulating EGR3.

Liver Cancer

Propidium iodide

Viability assay

10.1186/s12881-020-0974-9

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Citations (13)

Silencing of microRNA-210 inhibits the progression of liver cancer and Hepatitis B virus-associated liver cancer via targeting EGR3

Research Square (Research Square) (2020)

Xiaojie Li Mei Yuan Lu Song Yan Wang

Abstract Background: This study was aimed to investigate the regulatory role of microRNA-210 (miRNA-210) on the progression of liver cancer and Hepatitis B virus (HBV)-associated liver cancer. Methods: The expression of miRNA-210 was detected in liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells by qRT-PCR. MiRNA-210 was silenced in HepG2 and HepG2.2.15 cells by the transfection of miRNA-210 inhibitor. The cell viability and apoptosis was detected by MTT assay and Annexin V-fluorescein isothiocyanate/propidium iodide staining, respectively. The protein expression of EGR3 was detected by Western blot. The regulatory relationship between EGR3 and miRNA-210 was predicted by TargetScan and identified by Dual luciferase reporter gene assay. Results: MiRNA-210 was overexpressed in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells ( P < 0.05). Silencing of miRNA-210 inhibited the viability and promoted the apoptosis of HepG2 and HepG2.2.15 cells ( P < 0.05). EGR3 was a target of miRNA-210, which was down-regulated in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells ( P < 0.05). Silencing of miRNA-210 increased the mRNA and protein expression of EGR3 ( P < 0.05). Silencing of EGR3 reversed the anti-tumor effect of miRNA-210 inhibitor on HepG2 and HepG2.2.15 cells ( P < 0.05). Conclusions: Silencing of miRNA-210 inhibits the progression of liver cancer and HBV-associated liver cancer via up-regulating EGR3.

Propidium iodide

Liver Cancer

Viability assay

10.21203/rs.2.14458/v2

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APEX-mediated Proximity Labeling of Proteins in Cells Targeted by Extracellular Vesicles

BIO-PROTOCOL (2021)

Lu Song Jun Chen Angela Sun Randy Schekman

Extracellular vesicles (EVs) are thought to mediate intercellular communication through the delivery of cargo proteins and RNA to target cells. The uptake of EVs is often followed visually using lipophilic-dyes or fluorescently-tagged proteins to label membrane constituents that are then internalized into recipient cells ( Christianson et al., 2013 ; De Jong et al., 2019 ). However, these methods do not probe the exposure of EV cargo to intracellular compartments, such as the cytoplasm and nucleus, where protein or RNA molecules could elicit functional changes in recipient cells. In this protocol, we employ an EV cargo protein-APEX fusion to detect proximity interactions with recipient cell cytoplasmic/nuclear targets. This approach results in the biotinylation of proteins in close contact with the reporter fusion and thus permits profiling of biotinylated proteins affinity purified on immobilized streptavidin beads. Graphic abstract: Schematic showing three steps of APEX-mediated proximity labeling of proteins in cells targeted by EVs.

Streptavidin

Transport protein

Extracellular vesicles

10.21769/bioprotoc.4213

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Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1

The Journal of Cell Biology (2021)

Lu Song Xinran Tian Randy Schekman

Extracellular vesicles (EVs) are thought to mediate the transport of proteins and RNAs involved in intercellular communication. Here, we show dynamic changes in the buoyant density and abundance of EVs that are secreted by PC12 cells stimulated with nerve growth factor (NGF), N2A cells treated with retinoic acid to induce neural differentiation, and mouse embryonic stem cells (mESCs) differentiated into neuronal cells. EVs secreted from in vitro differentiated cells promote neural induction of mESCs. Cyclin D1 enriched within the EVs derived from differentiated neuronal cells contributes to this induction. EVs purified from cells overexpressing cyclin D1 are more potent in neural induction of mESC cells. Depletion of cyclin D1 from the EVs reduced the neural induction effect. Our results suggest that EVs regulate neural development through sorting of cyclin D1.

Neural Development

Neurosphere

10.1083/jcb.202101075

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Citations (26)

A case of false positive opiate immunoassay results from rifampin (rifampicin) treatment

Practical Laboratory Medicine (2023)

K. H. Brian Lam Lu Song Vincent Buggs Gopal Vithlani Imir G. Metushi

The drug screen test on a 12-year-old male patient was positive for opiates by a kinetic interaction of microparticles in solution (KIMS) immunoassay method on the Roche Cobas C502. The positive opiates result was not confirmed by the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. A chart review revealed that the patient had tuberculosis and was on rifampin. We spiked rifampin into drug-free urine and tested opiates with the Cobas method. Once again, a positive result was obtained. This case showed that rifampin can still cause false positive opiate results measured with the KIMS method. We want to stress the importance of confirming positive screen results by more specific methods such as LC-MS/MS.

Opiate

10.1016/j.plabm.2023.e00334

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Citations (1)