Rong Zhang

Second Affiliated Hospital of Zhejiang University

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Sheng Chen

Gaozhou People's Hospital

Hongwei Zhou

Second Affiliated Hospital of Zhejiang University

Edward Wai‐Chi Chan

Hong Kong Polytechnic University

Gong-Xiang Chen

Second Affiliated Hospital of Zhejiang University

Ning Dong

Zhejiang University

Qiaoling Sun

Zhejiang University

Jiachang Cai

Sichuan University

Yang Wang

China Agricultural University

Yanyan Hu

Florida State University

Congcong Liu

Second Affiliated Hospital of Zhejiang University

Cooperative Institutions

Chinese Academy of Sciences

245

Chinese Academy of Medical Sciences & Peking Union Medical College

189

Zhejiang University

184

Sun Yat-sen University

160

Shanghai Jiao Tong University

156

Peking University

145

Fudan University

141

China Agricultural University

133

Second Affiliated Hospital of Zhejiang University

125

Sichuan University

124

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The association of Ala54Thr variant of intestinal fatty acid binding protein gene with general and regional adipose tissue depots.

PubMed (1999)

Kun‐san Xiang Z Taisan Weiping Jia S Duoqi Wei D

To ascertain the relationship between the Ala54Thr variation of FABP2 gene and general as well as regional adipose tissue depots.165 subjects, in which 86 were subjects with normal glucose tolerance (NGT) [age 54.45+/-9.80, male/female 1.05, body mass index (BMI) 26.48+/-4.01] and 79 were subjects with non-insulin-dependent diabetes mellitus (NIDDM) (age 55.86+/-10.00, male/female 1.08, BMI 26.75+/-3.30).An association study of FABP2-Ala54Thr variation detected by PCR/HhaI digestion with general and regional adipose tissue depots determined by BMI and magnetic resonance imaging [abdominal subcutaneous and visceral adipose tissue area (SA and VA) and femoral subcutaneous adipose tissue area (FA)].The geneotype and allele frequencies of FABP2-Ala54Thr variation in Chinese were quite close to the frequencies in American Caucasians and Pima Indians reported in the literature. Significant difference in genotype frequency distribution was observed between FA subgroups comparisons (FA > or = 75 cm2 versus FA < 75 cm2) in NIDDM subjects (X2 = 11.460, P = 0.003), with significantly increased in Thr54-carrier [Thr54 (+)] genotype frequency and Thr54 allele frequency in NIDDM subject with FA < 75 cm2 (odd ratio for genotype was 4.62, X2 = 10.112, P = 0.001; and for allele = 2.36, X2 = 5.379, P = 0.020). The FA in NIDDM-Thr54 (+) subgroup was significantly lower than that in subjects with NIDDM-Thr54 (-) subgroup (61.19+/-21.51 cm2 versus 75.36+/-31.70 cm2, P = 0.021). Stepwise regression analysis revealed that FABP2-Thr54 genotype variation was an independent factor contributing to the variation of FA in NIDDM (P = 0.003).FABP2 is associated with regional adipose tissue depot. The decreased femoral subcutaneous adipose tissue depot in NIDDM subjects is related to FABP2-Thr54 variant.

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Author response for "Hypersensitive C‐reactive protein‐albumin ratio is associated with stroke‐associated pneumonia and early clinical outcomes in patients with acute ischemic stroke"

Lingling Huang Rong Zhang Jiahui Ji Fengdan Long Yadong Wang

Stroke

10.1002/brb3.2675/v2/response1

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The Thermal Conductivity of 3d Network Reinforced Polypropylene Matrix Composites Were Constructed by the Pickering-Like Emulsion Method

Zhoukai Zheng Yang Bai Chenyu Wang Shengfei Hu Rong Zhang

Download This Paper Open PDF in Browser Add Paper to My Library Share: Permalink Using these links will ensure access to this page indefinitely Copy URL Copy DOI

Polypropylene

Pickering emulsion

Matrix (chemical analysis)

10.2139/ssrn.4786700

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Genome-wide association studies in the Japanese population identify seven novel loci for type 2 diabetes

Nature Communications (2016)

Minako Imamura Atsushi Takahashi Toshimasa Yamauchi Kazuo Hara Kazuki Yasuda

Abstract Genome-wide association studies (GWAS) have identified more than 80 susceptibility loci for type 2 diabetes (T2D), but most of its heritability still remains to be elucidated. In this study, we conducted a meta-analysis of GWAS for T2D in the Japanese population. Combined data from discovery and subsequent validation analyses (23,399 T2D cases and 31,722 controls) identify 7 new loci with genome-wide significance ( P <5 × 10 −8 ), rs1116357 near CCDC85A , rs147538848 in FAM60A , rs1575972 near DMRTA1 , rs9309245 near ASB3 , rs67156297 near ATP8B2 , rs7107784 near MIR4686 and rs67839313 near INAFM2 . Of these, the association of 4 loci with T2D is replicated in multi-ethnic populations other than Japanese (up to 65,936 T2Ds and 158,030 controls, P <0.007). These results indicate that expansion of single ethnic GWAS is still useful to identify novel susceptibility loci to complex traits not only for ethnicity-specific loci but also for common loci across different ethnicities.

Genome-wide Association Study

Genetic Association

10.1038/ncomms10531

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Emergence of the clinical rdar morphotype carbapenem-resistant and hypervirulent Klebsiella pneumoniae with enhanced adaption to hospital environment

The Science of The Total Environment (2023)

Congcong Liu Ning Dong Xueting Huang Zixian Huang Chang Cai

Strain (injury)

10.1016/j.scitotenv.2023.164302

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Genome sequence of the ectophytic fungus Ramichloridium luteum reveals unique evolutionary adaptations to plant surface niche

BMC Genomics (2017)

Bo Wang Xiaofei Liang Mark L. Gleason Rong Zhang Guangyu Sun

Ectophytic fungi occupy the waxy plant surface, an extreme environment characterized by prolonged desiccation, nutrient limitation, and exposure to solar radiation. The nature of mechanisms that facilitate adaptation to this environment remains unclear. In this study, we sequenced the complete genome of an ectophytic fungus, Ramichloridium luteum, which colonizes the surface of apple fruit, and carried out comparative genomic and transcriptome analysis. The R. luteum genome was 28.18 Mb and encoded 9466 genes containing 1.85% repetitive elements. Compared with cell-penetrating pathogens, genes encoding plant cell wall degrading enzymes (PCWDEs), PTH11-like G protein-coupled receptors (GPCRs) and effectors were drastically reduced. In contrast, genes encoding cutinases and secretory lipases were strikingly expanded, and four of nine secretory lipases were probably acquired by horizontal gene transfer from Basidiomycota. Transcriptomic analysis revealed elevated expression of genes involved in cuticle degradation (cutinase, secretory lipase) and stress responses (melanin biosynthesis, aquaporins, lysozymes and HOG pathway). Taken together, our results highlight genomic features associated with evolution of surface niche adaptation by the ectophytic fungus R. luteum, namely the contraction of PCWDEs, PTH11-like GPCRs and effectors, and the expansion of cuticle degradation and stress tolerance.

Horizontal Gene Transfer

10.1186/s12864-017-4118-3

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The biological activity of antibacterial substance produced by Enterobacter cloacae B8.

PubMed (2003)

Yaping Jin Wei‐Liang Chen Rong Zhang Zhu Bai-rong

An antibacterial substance was extracted and purified from the culture of Enterobacter cloacae B8 and the antagonistic activities of the extract to 229 clinical pathogen strains was tested by the K-B paper-dish method. The extract of Enterobacter cloacae B8 showed strongly antagonistic activity to all 146 tested stains of Staphylococcus spp.; the antagonistic diameter was about 18-32 mm, averaging about 26.5 mm; showing especially strong antagonistic activity to all 87 tested strains of Methicillin-resistant Staphylococcus aureus (MRSA), the average antagonistic diameter was about 22.6 mm. The average antagonistic diameter of the extract of Enterobacter cloacae B8 to 3 tested strains of Escherchia coli and 8 tested strains of Klebsiella pneumoniae was about 14 mm and 13 mm, respectively; showing only antagonistic activities to 3 strains of Acinetobacter spp. in 26 tested strains, and showing no antagonistic activities to all 25 tested strains of Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) of partially purified extract against MRSA was about 9.4 micrograms/ml determined by disc-diffusion method, and the MIC of Vancomycin was about 3.1 micrograms/ml as the control in this study.

Enterobacter cloacae

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Linezolid resistance mechanisms and molecular epidemiology of clinical isolates of methicillin-resistant coagulase-negative staphylococci

Zhonghua weishengwuxue he mianyixue zazhi (2012)

Jiachang Cai Hongwei Zhou Yanyan Hu Rong Zhang Gong-Xiang Chen

Objective To investigate the linezolid resistance mechanisms and molecular epidemiology of clinical isolates of methicillin-resistant coagulase-negative staphylococci (MRCoNS).Methods Seventeen MRCoNS,including 10 S.capitis,4 S.cohnii,2 S.haemolyticus,and 1 S.sciuri with various levels of linezolid resistance were isolated from intensive care units in our hospital from March to August 2011. Minimal inhibitory concentration (MIC) was determined by E-test method. Pulsed-field gel electrophoresis was performed to analyze the molecular epidemiology.PCRs and DNA sequencing were preformed to investigate the mechanisms of linezolid resistance in MRCoNS.Results Nine S.capitis with linezolid MIC of ＞256 μg/ml were indistinguishable,and another S.capitis with linezolid MIC of 4 μg/ml was closely related.Four S.cohnii with linezolid MIC of ＞256 μg/ml were belonged to the same clonal strain.MIC of linezolid for S.sciuri was 64 μg/ml,and were 4 μg/ml and 6 μg/ml for 2 S.haemolyticus,respectively.A commom G2576T mutation and a novel C2104T mutation were identified in 9 S.capitis with linezolid MIC of ＞256 μg/ml by DNA sequence analysis of domain V of the 23S rRNA gene.cfr gene was deteeted in all staphylococci except a S.sciuri whose 23S rRNA gene contained the G2576T mutation.Conclusion It is the first report of linezolid-resistant clinical isolates of staphylococci in China.Linezolid resistance in MRCoNS is related to the presence of DNA mutation in domain V of the 23S rRNA gene and cfr gene.It's a clonally dissemination of linezolid-resistant MRCoNS in intensive care units of our hospital. Key words: Staphylococci; Linezolid; Antibiotic resistance; 23S rRNA; cfr

Linezolid

23S ribosomal RNA

Coagulase

Staphylococcus haemolyticus

SCCmec

10.3760/cma.j.issn.0254-5101.2012.06.010

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Licorice-saponin A3 is a broad-spectrum inhibitor for COVID-19 by targeting viral spike and anti-inflammation

Journal of Pharmaceutical Analysis (2023)

Yi Yang Wenzhe Li Kefang Liu Heng Xue Rong Yu

Currently, human health due to corona virus disease 2019 (COVID-19) pandemic has been seriously threatened. The coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein plays a crucial role in virus transmission and several S-based therapeutic approaches have been approved for the treatment of COVID-19. However, the efficacy is compromised by the SARS-CoV-2 evolvement and mutation. Here we report the SARS-CoV-2 S protein receptor-binding domain (RBD) inhibitor licorice-saponin A3 (A3) could widely inhibit RBD of SARS-CoV-2 variants, including Beta, Delta, and Omicron BA.1, XBB and BQ1.1. Furthermore, A3 could potently inhibit SARS-CoV-2 Omicron virus in Vero E6 cells, with EC50 of 1.016 μM. The mechanism was related with binding with Y453 of RBD determined by hydrogen-deuterium exchange mass spectrometry (HDX-MS) analysis combined with quantum mechanics/molecular mechanics (QM/MM) simulations. Interestingly, phosphoproteomics analysis and multi fluorescent immunohistochemistry (mIHC) respectively indicated that A3 also inhibits host inflammation by directly modulating the JNK and p38 MAPK pathways and rebalancing the corresponding immune dysregulation. This work supports A3 as a promising broad-spectrum small molecule drug candidate for COVID-19.

Vero cell

Coronavirus

10.1016/j.jpha.2023.05.011

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Structural and mechanistic basis of the high catalytic activity of monooxygenase Tet(X4) on tigecycline

BMC Biology (2021)

Qipeng Cheng Yan Chu Cheung Chenyu Liu Qingjie Xiao Bo Sun

Abstract Background Tigecycline is a tetracycline derivative that constitutes one of the last-resort antibiotics used clinically to treat infections caused by both multiple drug-resistant (MDR) Gram-negative and Gram-positive bacteria. Resistance to this drug is often caused by chromosome-encoding mechanisms including over-expression of efflux pumps and ribosome protection. However, a number of variants of the flavin adenine dinucleotide (FAD)-dependent monooxygenase TetX, such as Tet(X4), emerged in recent years as conferring resistance to tigecycline in strains of Enterobacteriaceae, Acinetobacter sp., Pseudomonas sp., and Empedobacter sp. To date, mechanistic details underlying the improvement of catalytic activities of new TetX enzymes are not available. Results In this study, we found that Tet(X4) exhibited higher affinity and catalytic efficiency toward tigecycline when compared to Tet(X2), resulting in the expression of phenotypic tigecycline resistance in E. coli strains bearing the tet (X4) gene. Comparison between the structures of Tet(X4) and Tet(X4)-tigecycline complex and those of Tet(X2) showed that they shared an identical FAD-binding site and that the FAD and tigecycline adopted similar conformation in the catalytic pocket. Although the amino acid changes in Tet(X4) are not pivotal residues for FAD binding and substrate recognition, such substitutions caused the refolding of several alpha helixes and beta sheets in the secondary structure of the substrate-binding domain of Tet(X4), resulting in the formation of a larger number of loops in the structure. These changes in turn render the substrate-binding domain of Tet(X4) more flexible and efficient in capturing substrate molecules, thereby improving catalytic efficiency. Conclusions Our works provide a better understanding of the molecular recognition of tigecycline by the TetX enzymes; these findings can help guide the rational design of the next-generation tetracycline antibiotics that can resist inactivation of the TetX variants.

Tigecycline

10.1186/s12915-021-01199-7

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Citations (10)