<div>Abstract<p>Circular RNAs (circRNA) are a new member of endogenously produced noncoding RNAs that have been characterized as key regulators of gene expression in a variety of malignances. However, the role of circRNA in oral squamous cell carcinoma (OSCC) remains largely unknown. In this study, we identified unique circRNA that regulate OSCC progression and metastasis and pave roads for future research in early diagnosis, prevention, and treatment of OSCC. Transcriptomic analyses identified a circRNA derived from <i>IGHG</i> locus (<i>circIGHG</i>) as significantly upregulated in OSCC and positively associated with poor prognosis of OSCC. <i>circIGHG</i> directly bound <i>miR-142-5p</i> and consequently elevated IGF2BP3 activity. Knockdown of <i>circIGHG</i> led to impaired expression of IGF2BP3 and attenuated aggressiveness of OSCC cells. Epithelial–mesenchymal transition was the main mechanism through which <i>circIGHG</i>/IGF2BP3 promotes metastasis of OSCC. Overall, these results demonstrate that circIGHG plays a pivotal role in OSCC development and metastasis and has potential to serve as a biomarker and therapeutic target for early-stage diagnosis and treatment of OSCC.</p>Significance:<p>These findings broaden our insights regarding regulation of OSCC progression by circular RNA and serve as a reference for future clinical research in OSCC diagnosis and treatment.</p></div>
The increasing emergence of pathogenic bacterial infections, especially multidrug-resistant (MDR) bacteria, has been regarded as an urgent threat. In addition, the invention of safe and effective methods for in-time bacterial diagnosis and treatment remains a considerable problem. To surmount this challenge, a multimode and broad-spectrum antimicrobial system based on cationic polypeptides (PG) functionalized meso-tetra(4-carboxyphenyl)-porphine (TCPP) and in situ self-assembled strategy were established to integrate the bioimaging, electrostatic targeting, and photodynamic antibacterial therapies. TCPP coordinated with cationic PG to facilitate the construction of water-soluble TCPP-PG nanoparticles (NPs) that avoid the self-aggregation of porphyrins and improve their dispersibility in an aqueous solution to ensure the reactive oxygen species (ROS) yield under irradiation for efficient bacterial inactivation. In addition, through the introduction of aqueous gold ions into bacterial cells, the metal precursors (i.e., HAuCl4) could be spontaneously in situ self-assembled to multifunctional gold nanoclusters (NCs) exhibiting luminescence and promoted the image sensitivity and specificity toward the bacterial. As a consequence, besides visualizing the bacteria, the constructed theranostic nanoplatform enabled the sterilization of both Gram-negative and Gram-positive bacteria under white light irradiation while displaying nontoxic against mammalian and red blood cells as reflected from their no significant histopathological toxicity, higher cell viability, and negligible hemolytic effect. This strategy may improve the possibility of establishment of a unique broad-spectrum antimicrobial routes that with significant potential for sterilization of intractable bacterial infections.
Aim: The aim was to highlight the clinical complications and to evaluate risk factors of mortality in Chinese HIV/TB patients. Methods: The etiology of clinical deterioration of Chinese HIV/TB patients were evaluated in 180 HIV-infected patients admitted in the Beijing Ditan Hospital between 1 January 2012 and 30 April 2014. Results & conclusion: AIDS-defining illnesses (20.0%) were the most common complication, followed by TB-associated immune reconstitution inflammatory syndrome (16.6%), drug-induced liver injury (11.1%), drug rash (11.1%), non-AIDS-defining illness (5.6%), as well as highly active antiretroviral therapy resistance (3.3%). The risk factors for mortality were tuberculous meningitis associated immune reconstitution inflammatory syndrome (OR: 152.614; CI: 18.324–1263.615; p < 0.001) and non-AIDS-defining illnesses (OR: 114.133; CI: 12.939–1006.752; p < 0.001), which will help remind physicians of the risk of clinical deterioration in HIV/TB patients after antiretroviral therapy in China.
Abstract Background In HIV-1 infection, more than 95% of CD4 + T cells die of caspase-1 mediated pyroptosis. What governs the increased susceptibility of CD4 + T cells to pyroptosis is poorly understood. Methods Blood samples were obtained from 31 untreated HIV-infected patients (UNT), 29 antiretroviral therapy treated HIV-infected patients (ART), and 21 healthy control donors (HD). Plasma levels of IL-18 and IL-1β, caspase-1 expression, mitochondrial mass (MM) and mitochondrial fusion/fisson genes of CD4 + T subsets were measured. Results A significantly higher IL-18 level in plasma and MM level of CD4 + T cells were found in HIV-infected patients (UNT and ART) compared to HD, and the MM high phenotype was manifested, related to increased caspase-1 expression. Moreover, the increased MM was more pronounced in the early differentiated and inactivated CD4 + T cells. However, higher MM was not intrinsically linked to T cell differentiation disorder or excessive activation of the CD4 + T cells. Mechanistically, the increased MM was significantly correlated with an elevated level of expression of the mitochondrial fusion gene mitofusin1 . Conclusion An increase in MM was associated with heightened sensitivity of CD4 + T cells to pyroptosis, even in early differentiated and inactivated CD4 + T cells, in patients with HIV-1 infection, regardless of whether patients were on antiretroviral therapy or not. These new revelations have uncovered a previously unappreciated challenge to immune reconstitution with antiretroviral therapy.
Autism spectrum disorder (ASD) is a widespread developmental disorder of the nervous system with an unclear etiology and pathogenesis. Its global incidence is currently increasing, and no effective drugs are available to improve its core symptoms. Nonpharmaceutical therapy can effectively relieve the core symptoms of autism, has fewer side effects than drugs, and is easily accepted by patients. This systematic and network meta-analysis aimed to evaluate the impact of non-pharmaceutical therapy on autism to explore preferable therapeutic options for autism.Online databases, including the China National Knowledge Infrastructure [CNKI], SinoMed, Wanfang Database [WF], China Science and Technology Journal Database [VIP], MEDLINE, Web of Science, EMBASE, and the Cochrane Library will be searched for randomized controlled trials of nonpharmacological interventions for autism published before October 2021. Two researchers will be independently responsible for the literature screening, data extraction, and quality assessment. Standard paired and Bayesian network meta-analyses will be performed using RevMan 5.3 Software and GEMTC 0.14.3, to compare the efficacy and safety of different nonpharmacological regimens.The results of this systematic and network meta-analysis will be submitted to a peer-reviewed journal for publication.This study provides a comprehensive and reliable evidence-based reference for the efficacy and safety of different non-pharmacological interventions for autism.CRD 42021275571.
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