Rui Zhang

Harbin Medical University

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Jundong Jiao

Harbin Medical University

Chang‐Hui Liao

China Pharmaceutical University

Kexin Meng

Chinese Academy of Medical Sciences & Peking Union Medical College

Ye-Ping Ren

Beijing Institute of Technology

Xiaohui Zhang

Peking University

Menghua Chen

Guangxi Medical University

Huiping Zhao

Peking University

Tongying Zhu

Peking University People's Hospital

Qingfeng Han

Peking University

Jie Dong

Peking University First Hospital

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Harbin Medical University

Second Affiliated Hospital of Harbin Medical University

Peking University

Chinese Academy of Sciences

Peking University People's Hospital

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

Sichuan University

Sun Yat-sen University

Army Medical University

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Prevalence of chromosomal abnormalities identified by copy number variation sequencing in high-risk pregnancies, spontaneous abortions, and suspected genetic disorders

Journal of International Medical Research (2019)

Rui Zhang Xiangbin Chen Dong Wang Xuan Chen Chao Wang

Objective High-throughput sequencing based on copy number variation (CNV-seq) is commonly used to detect chromosomal abnormalities including aneuploidy. This study provides evidence for the prevalence of chromosomal abnormalities in target populations. Methods A total of 160 samples, including 83 high-risk pregnancies, 37 spontaneous abortions, and 40 suspected genetic disorders, were analyzed by CNV-seq. Relationships between the incidence of these chromosomal abnormalities and risk factors (e.g. advanced maternal age, abnormal pregnancy history, and family history of congenital disease) were further analyzed by subgroup. Results A total of 37 (44.6%) high-risk pregnancies, 25 (67.6%) spontaneous abortions, and 22 (55%) suspected genetic disorders had chromosomal abnormalities including aneuploidy and CNVs. There was an increased risk association between the prevalence of aneuploidy and pathogenic-relevant CNV in the fetus or abortive tissue and advanced maternal age. Moreover, a family history of congenital disease was also positively correlated with fetal chromosomal abnormalities in high-risk pregnancies. Conclusion A relatively high prevalence of chromosomal abnormalities was detected in high-risk pregnancies, spontaneous abortions, and suspected genetic disorders, indicating the importance of CNV detection in such populations.

Advanced maternal age

Trisomy

10.1177/0300060518818020

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Citations (27)

Crystal Structure of HugZ, a Novel Heme Oxygenase from Helicobacter pylori

Journal of Biological Chemistry (2010)

Yonglin Hu Fan Jiang Ying Guo Xihui Shen Ying Zhang

The crystal structure of a heme oxygenase (HO) HugZ from Helicobacter pylori complexed with heme has been solved and refined at 1.8 Å resolution. HugZ is part of the iron acquisition mechanism of H. pylori, a major pathogen of human gastroenteric diseases. It is required for the adaptive colonization of H. pylori in hosts. Here, we report that HugZ is distinct from all other characterized HOs. It exists as a dimer in solution and in crystals, and the dimer adopts a split-barrel fold that is often found in FMN-binding proteins but has not been observed in hemoproteins. The heme is located at the intermonomer interface and is bound by both monomers. The heme iron is coordinated by the side chain of His245 and an azide molecule when it is present in crystallization conditions. Experiments show that Arg166, which is involved in azide binding, is essential for HugZ enzymatic activity, whereas His245, surprisingly, is not, implying that HugZ has an enzymatic mechanism distinct from other HOs. The placement of the azide corroborates the observed γ-meso specificity for the heme degradation reaction, in contrast to most known HOs that have α-meso specificity. We demonstrate through sequence and structural comparisons that HugZ belongs to a new heme-binding protein family with a split-barrel fold. Members of this family are widespread in pathogenic bacteria and may play important roles in the iron acquisition of these bacteria. The crystal structure of a heme oxygenase (HO) HugZ from Helicobacter pylori complexed with heme has been solved and refined at 1.8 Å resolution. HugZ is part of the iron acquisition mechanism of H. pylori, a major pathogen of human gastroenteric diseases. It is required for the adaptive colonization of H. pylori in hosts. Here, we report that HugZ is distinct from all other characterized HOs. It exists as a dimer in solution and in crystals, and the dimer adopts a split-barrel fold that is often found in FMN-binding proteins but has not been observed in hemoproteins. The heme is located at the intermonomer interface and is bound by both monomers. The heme iron is coordinated by the side chain of His245 and an azide molecule when it is present in crystallization conditions. Experiments show that Arg166, which is involved in azide binding, is essential for HugZ enzymatic activity, whereas His245, surprisingly, is not, implying that HugZ has an enzymatic mechanism distinct from other HOs. The placement of the azide corroborates the observed γ-meso specificity for the heme degradation reaction, in contrast to most known HOs that have α-meso specificity. We demonstrate through sequence and structural comparisons that HugZ belongs to a new heme-binding protein family with a split-barrel fold. Members of this family are widespread in pathogenic bacteria and may play important roles in the iron acquisition of these bacteria.

10.1074/jbc.m110.172007

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Citations (46)

Chronic hypoxia in cultured human podocytes inhibits BKCa channels by upregulating its β4-subunit

Biochemical and Biophysical Research Communications (2012)

Rui Zhang Hui Sun Chang‐Hui Liao Yang He Bo Zhao

Hypoxia

Pathogenesis

10.1016/j.bbrc.2012.03.021

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Citations (17)

High glucose-induced apoptosis in cultured podocytes involves TRPC6-dependent calcium entry via the RhoA/ROCK pathway

Biochemical and Biophysical Research Communications (2013)

Yang He Bo Zhao Chang‐Hui Liao Rui Zhang Kexin Meng

TRPC6

10.1016/j.bbrc.2013.03.087

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Citations (58)

Diabetic Patients Could Do As Well as Non-Diabetic Patients without Inflammation on Peritoneal Dialysis

PLoS ONE (2013)

Rong Xu Qingfeng Han Tongying Zhu Ye-Ping Ren Jianghua Chen

BackgroundDiabetic patients on peritoneal dialysis (PD) have lower survival and are more likely complicated with inflammation than their non-diabetic counterparts. Here, we explored the interaction effects between diabetes and inflammation on the survival of PD patients. MethodsOverall, 2,264 incident patients were enrolled from a retrospective cohort study in China. Patients were grouped according to the baseline levels of high-sensitive C-reactive protein (hsCRP, ≤3 mg/L or >3 mg/L) or serum albumin (SA, ≥38 g/L or <38 g/L). Then, several multivariable adjusted stratified Cox regression models were constructed for these groups to explore the predicted role of diabetes on all-cause or cardiovascular death under inflammatory or non-inflammatory conditions. ResultsDiabetics on PD were more likely to have inflammation than non-diabetics on PD, and they presented with elevated hsCRP (52.7% vs. 47.3%, P = 0.03) or decreased SA (77.9% vs. 62.7%, P < 0.001) levels. After stratification by size of center and controlling for confounding factors, diabetes was found to predict all-cause death in patients with hsCRP >3 mg/L or SA <38 g/L but not in patients with hsCRP ≤3 mg/L or SA ≥38 g/L. Similarly, the presence of diabetes was an indication of cardiovascular death in patients with hsCRP >3 mg/L or SA <38 g/L. However, if further adjusted by baseline cardiovascular disease, the predicted role of diabetes on death related to cardiovascular disease in patients with SA <38 g/L disappeared. ConclusionDiabetic patients could do as well as non-diabetic patients without inflammation on peritoneal dialysis. Active strategies should be implemented to improve inflammation status in diabetic patients on PD.

10.1371/journal.pone.0080486

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Citations (7)

The role of TRPC6 in oxidative stress-induced podocyte ischemic injury

Biochemical and Biophysical Research Communications (2015)

Bo Zhao Yang He Rui Zhang Hui Sun Chang‐Hui Liao

TRPC6

Hypoxia

Renal ischemia

10.1016/j.bbrc.2015.04.054

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Citations (30)

Functional Recovery from Damage of Intestinal Barrier and Ion Pump by Pingwei Powder in Rats with Syndrom of Dampness Blocking Spleen and Stomach

Zhongguo shiyan fangjixue zazhi (2010)

Rui Zhang

Objective: To explore the damage of intestinal barrier in the rats model of dampness blocking spleen and stomach syndrome and the functional recovery of Pingwei powder for it.Method: SD rats were randomly divided into control group,model group,treatment group and autonomous recovery group,ten rats in each.Animal model of dampness blocking spleen and stomach was established.Such indexes as content of D-lactic acid and activities of DAO,Na +-K +-ATPase and Ca2 +-ATPase were tested.Result: Compared with the blank group,the content of D-lactic acid increased(P 0.01) and the activities of Na +-K +-ATPase and Ca2 +-ATPase decreased obviously(P 0.01)except DAO(P 0.05).The general trend in Pingwei powder group treatment was better than that in natural recovery.Conclusion: Pingwei Powder can markedly recovery the damage of intestinal barrier and ion pump in rats with syndrom of dampness blocking spleen and stomach.

Blocking (statistics)

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Modeling studies with Helicobacter pylori octaprenyl pyrophosphate synthase reveal the enzymatic mechanism of trans-prenyltransferases

The International Journal of Biochemistry & Cell Biology (2012)

Jinyong Zhang Xiaoli Zhang Rui Zhang Chao Wu Ying Guo

10.1016/j.biocel.2012.09.002

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Citations (3)

<p>Urinary C-Peptide Creatinine Ratio as a Non-Invasive Tool for Identifying Latent Autoimmune Diabetes in Adults (LADA)</p>

Diabetes Metabolic Syndrome and Obesity (2019)

Wei Liu Xingquan Huang Xiuying Zhang Xiaoling Cai Xueyao Han

Latent autoimmune diabetes in adults (LADA) is a slowly progressing form of immune-mediated diabetes that combines phenotypical features of both type 2 diabetes mellitus (T2DM) and type 1 diabetes mellitus (T1DM), meaning that accurate and early diagnosis of this subtype of diabetes is critical for optimal long-term management. Urinary C-peptide creatinine ratio (UCPCR) represents a non-invasive and practical method for assessing endogenous insulin production to facilitate diabetes classification. However, no study to date has reported the use of UCPCR in identifying LADA.A total of 574 subjects were included in our study (42 LADA, 61 T1DM, 471 T2DM). All participants were evaluated for UCPCR and underwent clinical and laboratory evaluations. UCPCR was compared among different subtypes of diabetes using multinomial regression analysis, and a receiver operating characteristic (ROC) curve was used to identify its performance in diagnosing LADA.UCPCR was lower in LADA (0.4±0.6 nmol/mmol) compared with T2DM (1.2±0.9 nmol/mmol), but higher than in T1DM (0.2±0.3 nmol/mmol) (p<0.05). The association between UCPCR and LADA remained significant after adjusting for gender, age, age at diagnosis, body mass index, high-density lipoprotein cholesterol, and triglyceride (OR, 95% confidence interval (CI), 0.29 (0.09, 0.95)). The ROC curve revealed an area under the curve of 0.835 (95% CI (0.742-0.928), p<0.001). The cut-off point for UCPCR ≤ 0.46 nmol/mmol was 82.1% for sensitivity and 76.7% for specificity in the diagnosis of LADA.UCPCR may represent a non-invasive, simple, and practical measurement of insulin secretion for early discrimination of LADA in routine clinical practice.

10.2147/dmso.s229675

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Citations (7)

Serum Albumin, but not Bilirubin, is Associated with Diabetic Chronic Vascular Complications in a Chinese Type 2 Diabetic Population

Scientific Reports (2019)

Yu Zhu Xiaoling Cai Yan Liu Mengdie Hu Lingli Zhou

Abstract To identify the factors associated with serum total bilirubin (STB) and determine whether STB is independently associated with diabetic retinopathy (DR) or diabetic kidney disease (DKD), 1,665 Chinese patients with type 2 diabetes (T2DM) (248 outpatients newly diagnosed with T2DM [NDM] and 1,417 inpatients previously diagnosed with T2DM [PDM]) were studied. Clinical and biochemical information was collected, and a single nucleotide polymorphism (rs6704078) of the UGT1A1 gene was genotyped in 1,059 individuals. Multiple linear regression showed that STB was associated with haemoglobin concentration, platelet count, and serum triglyceride concentration in NDM and PDM patients, and with serum albumin, duration of diabetes, and smoking in PDM patients. In patients with PDM, multiple logistic regression revealed that serum albumin was associated with DR (odds ratio [OR] = 0.92, 95% confidence interval [CI]: 0.87–0.96, p = 0.001) and DKD (OR = 0.93, 95% CI: 0.88–0.98, p = 0.005) after adjustment for STB, STB-related factors, and risk factors for DR and DKD. In addition, patients with the T allele of rs6704078 had higher STB (13.2 [10.4–17.9] μmol/L versus 11.8 (9.4–14.8) μmol/L; p < 0.001) and similar risks of DR or DKD to those without the T allele. Thus, serum albumin, but not STB, is associated with DR and DKD.

10.1038/s41598-019-48486-6

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Citations (25)