An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.

Peanut butter

Quantitative Analysis

10.3390/toxins9040137

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Citations (16)

Experimental and kinetic study of pyridine oxidation under the fuel-lean condition in a jet-stirred reactor

Combustion and Flame (2022)

Lingnan Wu Zhen‐Yu Tian Kai-Ru Jin Zhi-Hao Zheng Du Wang

10.1016/j.combustflame.2022.112042

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Citations (12)

Effects of second injection timing on combustion characteristics of the spark ignition direct injection gasoline engines with dimethyl ether enrichment in the intake port

Energy (2019)

Lei Shi Changwei Ji Shuofeng Wang Teng Su Xiaoyu Cong

Thermal efficiency

Dimethyl ether

Ignition timing

Mean effective pressure

10.1016/j.energy.2019.05.056

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Citations (28)

A Comparative Study of Energy-Saving and Emission-Reduction of Urban Heating Schemes

Applied Mechanics and Materials (2013)

Pu Yan Zheng Du Wang Xiu Ping Yao Yan Yuan

The urban heating is closely linked with peoples life. The chosen of heating scheme is affected by the index of energy-saving and emission-reduction. In this thesis the computational model of the indexes for different range were built and the indexes of four schemes were calculated. The results predicted the energy-saving and emission-reduction of each scheme and provided references for the government to formulate assessment criteria.

10.4028/www.scientific.net/amm.411-414.3023

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Gene Co-expression Network Analysis of the Comparative Transcriptome Identifies Hub Genes Associated With Resistance to Aspergillus flavus L. in Cultivated Peanut (Arachis hypogaea L.)

Frontiers in Plant Science (2022)

Mengjie Cui Suoyi Han Du Wang Muhammad Salman Haider Junjia Guo

Cultivated peanut (Arachis hypogaea L.), a cosmopolitan oil crop, is susceptible to a variety of pathogens, especially Aspergillus flavus L., which not only vastly reduce the quality of peanut products but also seriously threaten food safety for the contamination of aflatoxin. However, the key genes related to resistance to Aspergillus flavus L. in peanuts remain unclear. This study identifies hub genes positively associated with resistance to A. flavus in two genotypes by comparative transcriptome and weighted gene co-expression network analysis (WGCNA) method. Compared with susceptible genotype (Zhonghua 12, S), the rapid response to A. flavus and quick preparation for the translation of resistance-related genes in the resistant genotype (J-11, R) may be the drivers of its high resistance. WGCNA analysis revealed that 18 genes encoding pathogenesis-related proteins (PR10), 1-aminocyclopropane-1-carboxylate oxidase (ACO1), MAPK kinase, serine/threonine kinase (STK), pattern recognition receptors (PRRs), cytochrome P450, SNARE protein SYP121, pectinesterase, phosphatidylinositol transfer protein, and pentatricopeptide repeat (PPR) protein play major and active roles in peanut resistance to A. flavus. Collectively, this study provides new insight into resistance to A. flavus by employing WGCNA, and the identification of hub resistance-responsive genes may contribute to the development of resistant cultivars by molecular-assisted breeding.

Arachis hypogaea

10.3389/fpls.2022.899177

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Citations (18)

[Kinetic mechanism and characteristics researches for hydrazine-based NOx removal at moderate to high temperatures].

PubMed (2012)

Hong Liu De-zhen Chen Du Wang Su Huang

The kinetic mechanism of NOx reduction with hydrazine hydrate in selective non-catalytic reduction (SNCR) process was studied and verified by the experimental results, the dominant radicals and reactions were confirmed through sensitivity analysis and key parameters in SNCR process have been obtained. The results of numerical simulation based on the proposed mechanism showed that the effective temperatures for SNCR de-NOx process were bimodal distribution with the optimum temperatures being 650 degrees C and 975 degrees C respectively and the lower temperature window fell in the range of 597-747 degrees C; while the experimental results proved the bimodal distribution of effective temperature with peak values being 653 degrees C and 968 degrees C respectively and the lower temperature window was within the range of 587-707 degrees C for hydrazine-based SNCR process, Simulation results and experimental results matched well with respect to reaction behaviors versus temperature. Through sensitivity analysis it had been found that the most effective reaction to reduce NO was the decomposition reaction of N2H4 to NH2 and the most helpful radical was NH2. Compared to ammonia the temperature window of hydrazine-based SNCR process on the lower temperature side is more flexible for application. Decreased O2 concentration would move the temperature window to the right side, which was contrary to that of ammonia; while increased n(N2H4)/n(NO) moved the temperature window to the left side and NO, reduction efficiency was also improved. All of these results showed that hydrazine has lower temperature window than those traditional de-NOx reagents and it will be widely used in application of SNCR as a potential reductant for NOx reduction.

Hydrazine (antidepressant)

Atmospheric temperature range

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Citations (1)