Fang Chen

Xian Yang Central Hospital

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Fengshan Yu

China University of Petroleum, Beijing

Xiaofeng Xue

Chinese Academy of Agricultural Sciences

Liming Wu

Institute of Apiculture Research

Jing Zhao

University of Macau

Jinhui Zhou

Nanjing Tech University

Yi Li

Beijing Information Science & Technology University

Jinzhen Zhang

Institute of Apiculture Research

Lanhua Chen

Soochow University

Lanzhen Chen

Institute of Apiculture Research

Huanjun Peng

Southwest University

Cooperative Institutions

Chinese Academy of Sciences

Huazhong University of Science and Technology

Ministry of Agriculture and Rural Affairs

Sichuan University

Fudan University

Shanghai Jiao Tong University

Chinese Academy of Agricultural Sciences

China Agricultural University

Central South University

Chinese Academy of Medical Sciences & Peking Union Medical College

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Hierarchical Interconnected Porous Scaffolds with Regulated Interfacial Nanotopography Exhibit Antimicrobial, Alleviate Inflammation, Neovascularization, and Tissue Integration for Bone Regeneration

Biomaterials (2025)

Shirun Chu Linlong Li Jiahao Zhang J. Q. You Xiaolan Li

Nanotopography

Interconnectivity

Bone tissue

10.1016/j.biomaterials.2025.123186

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Determination of protocatechuic acid in CORTEX ACAN THOPANACIS by high performance liquid chromatography-four channel UV-Vis detection method.

Medicinal plant (2011)

Fang Chen Lijuan Feng

[Objective] To establish a method to determine the content of protocatechuic acid in CORTEX ACAN THOPANACIS.[Method] Content of protocatechuic acid in CORTEX ACAN THOPANACIS was determined by high performance liquid chromatography-four channel UV-Vis detection method.Chromatographic conditions were as follows:ODSC18 chromatographic column(250 mm×4.6 mm,5 μm) ,and buffer solution of methanol and sodium dihydrogen phosphate as the mobile phase(0.1 mol/L,15∶85,V/V,pH=2.5) .4 detection wavelengths were 245,260,275 and 290 nm with the quantitative detection wavelength of 260 nm.[Result] Protocatechuic acid had good linear relationship within the range of 0.5-6.0 mg/L(r=0.999 9) .The average recovery rate was 99.87% with RSD=2.11%.[Conclusion] The method was reliable,stable,and simple,and could be used for the content determination of protocatechuic acid in CORTEX ACAN THOPANACIS.

Protocatechuic acid

Quantitative Analysis

Buffer solution

Phosphate buffered saline

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Determination of Cd(II) in Water Samples by Summable Spectrophotometry of Positive-Negative Peaks after Cloud Point Extraction

Advanced materials research (2012)

Lin Gao Fang Chen Hao Yi Yang Gao Yan Guo Zhong

Cadmium has been determined by summable spectrophotometry of positive-negative peaks with cadion and Triton X-114 after cloud point preconcentration. Influence factors on cloud point extraction, color reaction and absorbance were investigated by studying the summation absorbance of color compounds at 480 nm and 570 nm. The sensitivity and absorbance of this method are at least five times higher when compared with that of usual cadion spectrophotometry due to the summation of positive and negative peak. The proposed method is sensitive, simple, accurate and environmentally-friendly in the determination of cadmium in environment water.

Absorbance

Cloud point

Spectrophotometry

10.4028/www.scientific.net/amr.573-574.48

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Citations (2)

Proteomic analysis of rice plasma membrane reveals proteins involved in early defense response to bacterial blight. Proteomics 7:1529-1539

Fang Chen Yuexing Yuan Qun Li HE Zu-Hua

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Extraction and Content Determination of Shikimic Acid in Pinus elliottii Engelm

Agricultural Science and Technology Hunan (2010)

Xie Ji-yun Xiaopeng Chen Zhirong Li Fang Chen Benchao Su

[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources. [Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC. The HPLC conditions were as follows:Alltima NH2 (5 μm,4.6 mm × 150.0 mm) column separation;the mobile phase was Hexane-2%H3PO4(90∶10); the flowing velocity was 1 ml/min; test wavelength was 213 nm,the width of belt was 16 nm; compare wavelength was 300 nm,the width of belt was 80 nm. [Result] By the single factor and orthogonal tests,the optimum conditions were found as follows:ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1∶25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%. [Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.

Shikimic acid

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An improved on-line solid phase extraction coupled HPLC–MS/MS system for quantification of Sifuvirtide in human plasma

Journal of Chromatography B (2010)

Qingqing Wang Shensi Xiang Yanbo Jia Lun Ou Fang Chen

Solid phase extraction

10.1016/j.jchromb.2010.05.015

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Citations (10)

Characterisations of carbon-fenced conductive silver nanowires-supported hierarchical polyaniline nanowires

Electrochimica Acta (2018)

Shaoyun Chen Xingying Zhang Ben Liu Hong Shi Fang Chen

10.1016/j.electacta.2018.09.143

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Citations (13)

Visible Light-Driven Membraneless Photocatalytic Fuel Cell toward Self-Powered Aptasensing of PCB77

Analytical Chemistry (2018)

Kai Yan Yuhan Zhu Weihao Ji Fang Chen Jingdong Zhang

Artificial photocatalytic systems have been extensively established to mimic the natural solar-energy conversion process for developing useful photoelectric devices. In this work, a membraneless, hydrogen peroxide (H2O2)-based photocatalytic fuel cell (PFC) operated under visible light was proposed and applied in self-powered sensing of 3,3′,4,4′-tetrachlorobiphenyl (PCB77). The PFC was comprised of a photoanode fabricated with gold nanoparticles-decorated graphitic C3N4 nanosheet and a cathode modified with hemin-graphene nanocomposites. The combination of both photocatalytic oxidation and electrochemical reduction of H2O2 processes led to electron transfer in the external circuit, which could generate a certain electric power output. Taking the advantage of the inhibited output performance of PFC by PCB77 which interacted with aptamer immobilize on the photoanode, a self-powered aptasensor driven by visible light was achieved, without applying an external electric source. Thus, a highly sensitive and selective self-powered aptasensor for PCB77 was successfully demonstrated.

Hemin

Visible spectrum

Nanosheet

10.1021/acs.analchem.8b02302

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Citations (65)

Determination of EDTA Based on the Fluorescence Recovery of 5-sulfosalicylic Acid-Fe~(3+) Complex

Journal of Hubei University for Nationalities (2011)

Fang Chen

A new spectrofluorimetric method was developed for determination of EDTA.The interaction between 5-sulfosalic acid and Fe3+ was studied by using UV-vis absorption and fluorescence spectras.It found that Fe3+ could quench the fluorescence of 5-sulfosalicylic acid and the fluorescence of 5-sulfosal-icylic acid-Fe3+ recovered when EDTA was added.Under the optimum conditions,the dynamic range forthe determination of EDTA was 1.0×10-6～1.2×10-5mol/L,and the detection limit(3σ/k) was 6.0×10-7mol/L.The relative standard deviation was 3.1% for the determination of 6.0×10-6mol/L EDTA(n=11).This method could be successfully applied to determination of EDTA in real water samples.

Sulfosalicylic acid

Relative standard deviation

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Analysis on the Volatile Oils from Rhododendron calophytum Franch by GC-MS

Fang Chen

Objective To analyze the chemical constituents of volatile oils from leaves of Rhoodendron calophytum Franch.Methods The volatile oils were extracted by steam distillation.The chemical compounds were separated and identified by capillary GC-MS method.The relative content of each component was calculated by area normalization.Results Sixty four compounds were identified from leaves of Rhododendron calophytum Franch.The ratio of identification of total compounds was 90.00%.The main components are 3,7,11,15-tetramethyl-2-hexadecen-1-ol(5.68%);1,3,5-Trimethylbenzene(5.53%);naphthalene,2,3,6-trimethy(4.75%);kaur-16-ene(3.66%);1-Nonadecene(2.88%);3-eicosyne(2.63%);Hemimellitene(2.46%);p-ethylguaiacol(2.39%);dibutyl phthalate(2.39%);naphthalene,2,6-dimethyl(2.20%).Conclusion The result of research provides a scientific method for the reasonable exploitation and utilization of Rhododendron calophytum Franch.

Dibutyl phthalate

Steam distillation

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