Xiao Xiao

East China University of Science and Technology

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Yaacov Ben‐David

Guiyang Medical University

Eldad Zacksenhaus

University of Toronto

Wuling Liu

Guiyang Medical University

Anling Hu

Guiyang Medical University

Babu Gajendran

Guiyang Medical University

Yi Kuang

Guiyang Medical University

Dao Wen Wang

Huazhong University of Science and Technology

Chunfang Gao

Yueyang Hospital

Chunlin Wang

Ningbo University

Darryl C. Zeldin

National Institute of Environmental Health Sciences

Cooperative Institutions

Huazhong University of Science and Technology

Sun Yat-sen University

Central South University

Guiyang Medical University

Shanghai Jiao Tong University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Fudan University

Southern Medical University

Sichuan University

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Artificial intelligence–assisted real-time monitoring of effective withdrawal time during colonoscopy: a novel quality marker of colonoscopy

Gastrointestinal Endoscopy (2023)

Thomas Ka-Luen Lui Michael Ko Jingjia Liu Xiao Xiao Wai K. Leung

Withdrawal time

10.1016/j.gie.2023.10.035

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Citations (4)

Two cultivated legume plants reveal the enrichment process of the microbiome in the rhizocompartments

Molecular Ecology (2017)

Xiao Xiao Weimin Chen Le Zong Jun Yang Shuo Jiao

The microbiomes of rhizocompartments (nodule endophytes, root endophytes, rhizosphere and root zone) in soya bean and alfalfa were analysed using high-throughput sequencing to investigate the interactions among legume species, microorganisms and soil types. A clear hierarchical filtration of microbiota by plants was observed in the four rhizocompartments - the nodule endosphere, root endosphere, rhizosphere and root zone - as demonstrated by significant variations in the composition of the microbial community in the different compartments. The rhizosphere and root zone microbial communities were largely influenced by soil type, and the nodule and root endophytes were primarily determined by plant species. Diverse microbes inhabited the root nodule endosphere, and the corresponding dominant symbiotic rhizobia belonged to Ensifer for alfalfa and Ensifer-Bradyrhizobium for soya bean. The nonsymbiotic nodule endophytes were mainly Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The variation in root microbial communities was also affected by the plant growth stage. In summary, this study demonstrated that the enrichment process of nodule endophytes follows a hierarchical filtration and that the bacterial communities in nodule endophytes vary according to the plant species.

Bradyrhizobium

Mesorhizobium

10.1111/mec.14027

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Citations (156)

The Astragalus Membranaceus Herb Attenuates Leukemia by Inhibiting the FLI1 Oncogene and Enhancing Anti-Tumor Immunity

International Journal of Molecular Sciences (2024)

Kunlin Yu Yaoliang Tang Chunlin Wang Wuling Liu Maoting Hu

Astragalus membranaceus (AM) herb is a component of traditional Chinese medicine used to treat various cancers. Herein, we demonstrate a strong anti-leukemic effect of AM injected (Ai) into the mouse model of erythroleukemia induced by Friend virus. Chemical analysis combined with mass spectrometry of AM/Ai identified the compounds Betulinic acid, Kaempferol, Hederagenin, and formononetin, all major mediators of leukemia inhibition in culture and in vivo. Docking analysis demonstrated binding of these four compounds to FLI1, resulting in downregulation of its targets, induction of apoptosis, differentiation, and suppression of cell proliferation. Chemical composition analysis identified other compounds previously known having anti-tumor activity independent of the FLI1 blockade. Among these, Astragaloside-A (As-A) has marginal effect on cells in culture, but strongly inhibits leukemogenesis in vivo, likely through improvement of anti-tumor immunity. Indeed, both IDO1 and TDO2 were identified as targets of As-A, leading to suppression of tryptophane-mediated Kyn production and leukemia suppression. Moreover, As-A interacts with histamine decarboxylase (HDC), leading to suppression of anti-inflammatory genes TNF, IL1B/IL1A, TNFAIP3, and CXCR2, but not IL6. These results implicate HDC as a novel immune checkpoint mediator, induced in the tumor microenvironment to promote leukemia. Functional analysis of AM components may allow development of combination therapy with optimal anti-leukemia effect.

Jurkat cells

10.3390/ijms252413426

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Citations (1)

Targeting Pancreatic Cancer Stem Cells

Journal of Cancer Sciences (2015)

Xiao Xiao Xiangwei Xiao

10.13188/2377-9292.1000005

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Citations (0)

Neuroprotective Effects of an Edible Pigment Brilliant Blue FCF against Behavioral Abnormity in MCAO Rats

Pharmaceuticals (2022)

Jingyang Le Xiao Xiao Difan Zhang Yi Feng Zhuoying Wu

Ischemic stroke leads to hypoxia-induced neuronal death and behavioral abnormity, and is a major cause of death in the modern society. However, the treatments of this disease are limited. Brilliant Blue FCF (BBF) is an edible pigment used in the food industry that with multiple aromatic rings and sulfonic acid groups in its structure. BBF and its derivatives were proved to cross the blood-brain barrier and have advantages on the therapy of neuropsychiatric diseases. In this study, BBF, but not its derivatives, significantly ameliorated chemical hypoxia-induced cell death in HT22 hippocampal neuronal cell line. Moreover, protective effects of BBF were attributed to the inhibition of the extracellular regulated protein kinase (ERK) and glycogen synthase kinase-3β (GSK3β) pathways as evidenced by Western blotting analysis and specific inhibitors. Furthermore, BBF significantly reduced neurological and behavioral abnormity, and decreased brain infarct volume and cerebral edema induced by middle cerebral artery occlusion/reperfusion (MCAO) in rats. MCAO-induced increase of p-ERK in ischemic penumbra was reduced by BBF in rats. These results suggested that BBF prevented chemical hypoxia-induced otoxicity and MCAO-induced behavioral abnormity via the inhibition of the ERK and GSK3β pathways, indicating the potential use of BBF for treating ischemic stroke.

Hypoxia

Penumbra

10.3390/ph15081018

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Citations (2)

Intramuscular delivery of rAAV-mediated kallikrein gene reduces hypertension and prevents cardiovascular injuries in model rats

Acta Pharmacologica Sinica (2007)

Tao Wang Ling-bo HOU Zhen-jun LIU Yan Wang Chun-lian CHEN

Intramuscular injection

10.1111/j.1745-7254.2007.00677.x

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Citations (9)

A novel uveitis model induced by lipopolysaccharide in zebrafish

Frontiers in Immunology (2022)

Xiao Xiao Zhangluxi Liu Guannan Su Huan Liu Wenhui Yin

Endotoxin-induced uveitis (EIU) is an important tool for human uveitis study. This study was designed to develop a novel EIU model in zebrafish. An EIU model in zebrafish was induced by intravitreal lipopolysaccharide (LPS) injection and was assessed dynamically. Optical coherence tomography (OCT) was used to assess infiltrating cells in the vitreous body. The histological changes wereevaluated using HE staining and immune cells were measured by immunofluorescence. The retinal RNA Sequencing (RNA-Seq) was used to explore the transcriptional changes during inflammation. RNA-Seq data were analyzed using time-course sequencing data analysis (TCseq), ClueGO plugin in Cytoscape, and Gene Set Enrichment Analysis (GSEA) software. Flow cytometry and retinal flat mounts were used to dynamically quantify the immune cells. EIU was successfully induced in zebrafish following intravitreal LPS injection. Inflammation appeared at 4 hours post injection (hpi), reached its peak at 24 hpi, and then resolved at 72 hpi. Immunofluorescence confirmed that massive influx ofneutrophils into the iris and vitreous body, and activation of microglia as evidenced by ameboid-shaped appearance in the retina. Retinal RNA-seq during the EIU course identified four gene clusters with distinct expression characteristics related to Toll-likereceptor signaling pathway, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, and extracellular matrix (ECM)-receptor interaction, respectively. Prednisone immersion inhibited the inflammatory response of EIU in zebrafish, whichwas confirmed by decreased neutrophils detected in flow cytometry and retinal flat mounts. We developed a novel EIU model in zebrafish, which may be particularly useful for gene-editing and high-throughput screening of new drugs for the prevention and treatment of uveitis.

Muller glia

10.3389/fimmu.2022.1042849

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Citations (2)

Erythropoietin

Springer eBooks (2014)

Yaacov Ben‐David Xiao Xiao

10.1007/978-3-642-27786-3_466-2

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Citations (0)

Dark Green Tea Processing Craft in Southen Shaanxi

Northern Horticulture (2012)

Xiao Xiao

With processing dark green tea by using fresh leaves produced in Autumn as raw material in Hanzhong Xixiang county.According to the sequence of fixation,rub twists,pile-fermentation and drying,and the national standard,used the ultraviolet specrophotometer,polyphenols,caffeine,amino acids,carbohyd rate and pigment were tested contains composition of processing dark green tea to pile-fermentation,to explore the better process parameters.The results showed that with the pile-fermentation prolonged,the main biochemical components had changed.At the end of the pile-fermentation,thearubigins(TR),theaflavins(TF) and theabrownin(TB) were greatly increased;polyphenols,amino acids,caffeine and carbohydrate reduced.It showed that the better process parameters of dark green tea were fixation's leaf∶water was 0.2∶1,fixation's weight leaves was 10~12 kg,weight leaves when rub twists was 28~30 kg,thickness of spreading and drying was 10 cm,water content when pile-fermentation was about 50%.It showed that thearubigins(TR),theaflavins(TF),theabrownin(TB) were greatly increased and tea polyphenols was reduced,it revealed quality of dark green tea's color and tastes,further proof that the processing of dark tea.

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Arachidonic Acid Epoxygenase Metabolites Stimulate Endothelial Cell Growth and Angiogenesis via Mitogen-Activated Protein Kinase and Phosphatidylinositol 3-Kinase/Akt Signaling Pathways

Journal of Pharmacology and Experimental Therapeutics (2005)

Yan Wang Xin Wei Xiao Xiao Rutai Hui Jeffrey W. Card

Cytochrome P450 arachidonic acid (AA) epoxygenase metabolites, the epoxyeicosatrienoic acids (EETs), dilate arteries via hyperpolarization of smooth muscle cells and also have nonvasodilatory effects within the vasculature. The present study investigated the angiogenic effects of endogenous and exogenous EETs and the relevant signaling mechanisms involved. Bovine aortic endothelial cells (BAECs) were incubated with synthetic EETs or infected with recombinant adeno-associated viruses (rAAVs) containing CYP2C11-NADPH-cytochrome P450 oxidoreductase (CYPOR), CYP2J2, or CYP102 F87V mutant to increase endogenous levels of EETs. The following endpoints were measured: BAEC proliferation, migration, capillary formation, and in vivo angiogenesis. The potential involvement of various signaling pathways was explored using selective inhibitors. The results showed that transfection with either rAAV-CYP2C11-CYPOR, rAAV-CYP2J2, or rAAV-CYP102 F87V, or incubation with EETs promoted BAEC proliferation, increased migration of BAECs as assessed by Transwell analysis and wound healing assays, and enhanced capillary tubule formation as determined by chicken embryo chorioallantoic membrane assays and tube formation tests on matrigel. The effects of EETs on proliferation, migration, and capillary tubule formation were attenuated by inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 (PI3)-kinase/Akt pathways and partially attenuated by an endothelial nitricoxide synthase (eNOS) inhibitor but not by a protein kinase C inhibitor. In a rat ischemic hind limb model, rAAV-mediated AA epoxygenase transfection induced angiogenesis. We conclude that AA epoxygenase metabolites can promote angiogenesis, which may provide protection to ischemic tissues. The results also suggest that the angiogenic effects of EETs involve the MAPK and PI3-kinase/Akt signaling pathways, and to some extent, the eNOS pathway.

Epoxygenase

Wortmannin

10.1124/jpet.105.083477

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Citations (199)