Li Feng

Ningxia Medical University

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Glenn D. Prestwich

University of Utah

Zhang Li

Bethune International Peace Hospital

Guangli Wang

Yanbian University

Stacy S. Hawkins

Unilever (United States)

Dayong Xu

Huaibei Normal University

Yuanyuan Zhai

Inner Mongolia University

Maozhi Ren

Chinese Academy of Agricultural Sciences

Tong Xie

Nanjing University of Chinese Medicine

Jinjun Shan

Nanjing University of Chinese Medicine

Minghua Xiong

China Academy of Space Technology

Cooperative Institutions

Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Huaibei Normal University

Shanghai Jiao Tong University

Huazhong University of Science and Technology

Sun Yat-sen University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Medical Sciences & Peking Union Medical College

Zhejiang University

University of Chinese Academy of Sciences

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Study on the Vacuum Freeze-drying Technology of Ginger Powder

Li Feng

This paper is based on,which takes the moisture content, rate ,hydratability and the content of gingerol as the influence target, takes the slice thickness, the precooling temperature, the drying time as the influencing factor, , selectes L9(34) orthogonal test to determine the vacuum freeze-drying ginger powder best technological parameter,which is material thickness 2mm, pre-chilling temperatures -36℃, drying time 36h. And we have carried on experiments between it and microwave drying and hot-air seasoning.Finally the result indicated that the vacuum freezing drying method surpasses other dry methods obviously on the ginger powder rate ,moisture content and the sense organ performance.

Seasoning

Vacuum drying

Vacuum packing

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YAP1 promotes the stemness of airway epithelial basal cells and spontaneous formation of lung squamous cell carcinoma in a YAP1KITrp53KO mouse model

Research Square (Research Square) (2023)

Xiewan Chen Chun-Li Jian L. Zhang Li Feng Xingyun Liao

Abstract Background Tumorigenic mechanisms and personalized therapeutic strategies for lung squamous cell carcinoma (LSCC) lack clarity. Practical LSCC animal models remain to be developed or improved. We aimed to determine whether Yes-associated protein-1 (YAP1) promotes the stemness of airway epithelial basal cells and LSCC tumor-initiating cells (TICs) and spontaneous tumorigenesis in a self-developed YAP1 knock-in/Trp53 knockout mouse model. Methods Airway epithelial basal cells and LSCC TICs were assessed for stemness by immunofluorescence (IF) staining and fluorescence-activated cell sorting. YAP1 expression patterns and levels were evaluated by IF and qRT-PCR. The effect of YAP1 on the tracheosphere-forming ability of airway epithelial basal cells was investigated by YAP1 overexpression and deletion, observed by 3D-matrigel. Homozygous YAP1 KI Trp53 KO mice were generated by a special vector design that introduced a ciliated cell-specific promoter FOXJ1. Tumor formation was determined by micro-CT scanning, and histological subtype was confirmed through hematoxylin-eosin (H&E) and immunohistochemical (IHC) staining. Results YAP1 promoted the stemness maintenance of airway epithelial basal cells. Overexpression and deletion of YAP1 increased and decreased the tracheosphere-forming ability of airway epithelial basal cells, respectively. YAP1 also contributed to the stemness of LSCC TICs. A homozygous YAP1 KI Trp53 KO LSCC mouse model was constructed successfully. After a period of feeding, cancer nests occurred spontaneously in the murine lung. H&E and IHC staining confirmed the LSCC histological subtype, and YAP1 was primarily expressed in the nucleus as evidence of active proliferation. Conclusions We established a YAP1 KI Trp53 KO mouse model of spontaneous LSCC, providing a convenient tool for investigating novel targets and therapies.

YAP1

Basal (medicine)

10.21203/rs.3.rs-3737010/v1

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The Use of Barley in Beer Brewing&Its Technology Control

Liquor-making Science & Technology (2007)

Li Feng

The addition of part barley instead of barley malt in beer brewing could save production cost and improve wort quality. Currently, most breweries grinded barley and corn together for dextrinization at 100 ℃ and then for saccharification. However, our experiments proved that it was unnecessary to dextrinize at 100 ℃ and saccharification could be operated directly without any additional enzyme. The proper adjustment of the techniques could use barley more effectively and save energy and equipment and increase economic benefits.

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Microwave pasteurization of pre-packaged carrots

Journal of Food Engineering (2017)

Jing Peng Juming Tang Donglei Luan Frank Liu Zhongwei Tang

Pasteurization

Brine

10.1016/j.jfoodeng.2017.01.003

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Characterization of two novel hydrolases from Sphingopyxis sp. DBS4 for enantioselective degradation of chiral herbicide diclofop-methyl

Journal of Hazardous Materials (2024)

Zhenbo Mao Man Song Ruiqi Zhao Yuan Liu Yumeng Zhu

Amidase

10.1016/j.jhazmat.2024.133967

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全ゲノム配列決定によるバルク分離分析を組み合わせることによるイネ(Oryza sativa L.)における種子破砕のための遺伝子座の同定【JST・京大機械翻訳】

Molecular Breeding (2019)

Li Feng Hisataka Numa Naho Hara Naoki Sentoku T. Ishii

Plant Physiology

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Modulators of lysophosphatidic acid signalling

Expert Opinion on Therapeutic Patents (2003)

Li Feng Gordon B. Mills Glenn D. Prestwich

Lysophosphatidic acid (LPA) is a key lipid mediator in the regulation of cell proliferation, cell survival, motility, invasion and wound healing in normal cells, such as fibroblasts and haematopoietic cells. In addition, LPA signalling is implicated in cancer, atherosclerosis, ischaemia perfusion injury and other pathophysiological conditions. LPA, sphingolipids and other lysophospholipids act through several mechanisms: (i) a family of cell-surface seven transmembrane domain G-protein-coupled receptors; (ii) a nuclear hormone-activated transcription factor; (iii) membrane curvature and endocytosis and (iv) other targets yet to be defined. Based on the action of LPA on molecular targets in different human pathologies, both receptor-selective agonists and antagonists are sought as potential clinical agents. In addition, the control of endogenous production and clearance of LPA may provide an important target for treatment of multiple disease states. Thus, modifiers of phospholipase A1 and A2, lysophospholipase D, LPA acyl transferase and lipid phosphate phosphatase activities should be explored as potential therapeutics. This overview summarises the literature and issued patents covering the molecular agents developed to potentially manipulate the specific effects of LPA on cell physiology and clinical outcome.

Autotaxin

Phospholipase D

10.1517/13543776.13.10.1619

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Effect of tocotrienol rich fraction of palm oil on glucose metabolism in atherosclerotic mice

Zhongguo yaolixue yu dulixue zazhi (2009)

Li Feng

AIM To investigate the effect of tocotrienol rich fraction of palm oil(TRF) on glucose metabolism in atherosclerotic mice and the possible mechanism.METHODS Apolipoprotein E gene deficient(ApoE-/-) mice were divided into 3 groups as model control,TRF 0.05% and 0.2%(W/W) groups.10%(W/W) fat and 0.2%(W/W) cholesterol were added into the diets to induce atherosclerosis formation.Oral glucose tolerance test and insulin tolerance test were conducted after mice were treated by TRF for 12 and 14 weeks respectively.Serum cholesterol,triglyceride,free fatty acid,and insulin levels were measured using corresponding kits.The mRNA expression levels for peroxisome proliferator-activated receptor γ(PPARγ),adiponectin and glucose transporter 4(Glut4) in white adipose tissue(WAT) were determined by using quantitative real-time PCR.Activation of PPARγ by TRF was tested using luciferase reporter assays.RESULTS Compared with the model control group,TRF decreased non-fasting or fasting blood glucose levels and improved insulin sensitivity of ApoE-/-mice.Both TRF groups showed decreased levels of triglyceride and free fatty acid.The mRNA level of adiponectin in WAT was up-regulated by(1.73±0.32) times in TRF 0.2% group compared with the control group.Glut4 mRNA level was increased(1.89±0.24) and(2.01±0.61) times compared with control group in TRF 0.05% group and TRF 0.2% group respectively.The fold inductions of TRF on PPARγ-ligand-binding domain,PPARγ1 and PPARγ2 activities were(2.7±0.2),(6.1±0.65) and(5.3±0.1) times compared with DMSO by using luciferase reporter assay.CONCLUSION TRF can improve glucose metabolism in atherosclerotic mice and this effect may be partly due to modulating the activity of PPARγ.

GLUT4

Carbohydrate Metabolism

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Study on the melt resistance of jellied meat

Science and Technology of Food Industry (2008)

Li Feng

Tissue transglutaminase

Gelatin

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Metabolomics combined with proteomics analysis of femur provides a comprehensive interpretation of the changes in postmenopausal osteoporosis under salidroside treatment

Pharmacological Research - Modern Chinese Medicine (2022)

Yuanyuan Zhai Xin Li Yifei Wang Mengting Gao Li Feng

Salidroside (SA), a primary biologically active compound in Fructus Ligustri Lucidi, has a definite effect on anti-aging, a physiological phenomenon that is directly related to postmenopausal osteoporosis (PMOP). However, the mechanisms of treating PMOP of SA have not been comprehensively investigated. In this study, metabolomics combined with proteomics analysis of femur was established to uncover the detailed molecular mechanism of SA exerted on PMOP. Firstly, we established the mice models of PMOP and detected the changes of biochemical indices in serum and histomorphometric analysis in femur to explore the effects of salidroside (SA) in treating the disease. Then, the gas chromatography-mass spectrometry (GC-MS) based metabolomics and Tandem Mass Tag (TMT) labeled proteomics of femur samples have been developed to find the possible significant metabolites and proteins in PMOP mice and all difference alters were further integrated to find the potential pathways related to PMOP. Next, network analysis was applied to visualize the relationships between identified metabolites and proteins in detail to systematically understand the pathological mechanism of PMOP at a molecular level. Finally, crucial proteins were verified and it might provide a foundation for making out the potential sensitive targets on PMOP. This study demonstrated that SA might prevent the pathological process of PMOP through regulating the disturbed metabolic pathway, including glucose metabolism, lipid metabolism and amino acid metabolism. In addition, the proteins of GLB1 and B4GALT1 were further verified by Western blot and ELISA. Meanwhile, GLB1 as an enzyme related to aging might be a potential marker for PMOP. Our integrated proteomics/metabolomics study of femur samples could contribute to systematically understand the pathological mechanism of PMOP at a molecular level and might provide a foundation for making out the potential sensitive targets of SA on PMOP via anti-aging.

10.1016/j.prmcm.2022.100079

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