Lei Wang

Affiliated Hospital of Nantong University

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Yujie Feng

Harbin Institute of Technology

Baoqiang Li

Harbin Institute of Technology

Daqing Wei

Harbin Institute of Technology

Dechang Jia

Harbin Institute of Technology

Lu‐Ning Wang

University of Science and Technology Beijing

Feng Xu

Xi'an Jiaotong University

Kun Yang

Hebei University

Haiyu Wang

Gansu University of Traditional Chinese Medicine

Xuehui Zhang

Kunming Medical University

Yu Zhou

Jiujiang University

Cooperative Institutions

Chinese Academy of Sciences

Shanghai Jiao Tong University

University of Science and Technology Beijing

Tsinghua University

Peking University

Zhejiang University

Jilin University

Soochow University

Harbin Institute of Technology

Chinese Academy of Medical Sciences & Peking Union Medical College

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Highly Photoluminescent Carbon Dots for Multicolor Patterning, Sensors, and Bioimaging

Angewandte Chemie International Edition (2013)

Shoujun Zhu Qingnan Meng Lei Wang Junhu Zhang Yubin Song

Shine on you crazy dots: A rapid and high-output strategy allows the fabrication of polymer-like carbon dots (CDs) with quantum yields as high as ca. 80 %. This value is the highest reported to date for fluorescent carbon-based materials, and gives promise for their application in multicolor-patterning and biosensors. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

Carbon fibers

10.1002/anie.201300519

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Citations (3,359)

Smartphone-Based Photothermal Lateral Flow Immunoassay Using Rhenium Diselenide Nanosheet

ACS Applied Materials & Interfaces (2023)

Wenbin Hao Yan Huang Lei Wang Jing Liang Shuangshuang Yang

Developing a rapid antibody-based detection method is of great importance for preventing and controlling the spread of coronavirus disease 2019 (COVID-19). Among the antibody-based methods for point-of-care (POC) detection, lateral flow immunoassay (LFIA) is the most widely used. However, LFIA still has the disadvantage of low sensitivity. In this work, an ReSe2 nanosheet with a thickness of 10–20 nm was prepared by liquid exfoliation and applied as the label in a photothermal LFIA due to its high photothermal conversion efficiency and high photothermal stability. An integrated detection device was introduced for rapid, on-site, and highly sensitive assay of the human antisevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike (S) protein IgG antibodies. The device mainly included a rhenium diselenide (ReSe2) nanosheet-based photothermal LFIA, a portable laser, and a smartphone with a portable thermal imager, which was used to record and analyze the thermal signal of the LFIA test zone. The human anti-SARS-COV-2 S protein IgG antibodies in buffer solution can be detected in a portable box within 10 min, with a thermal signal detection limit of 0.86 ng mL–1, which was 108-fold lower than that of the colorimetric signal. The integrated device can detect values as low as 2.76 ng mL–1 of the human anti-SARS-COV-2 S protein IgG antibodies in 50% serum. The integrated device showed great potential for rapid and home self-testing diagnosis of COVID-19.

Nanosheet

10.1021/acsami.2c22616

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Citations (29)

A general route to make non-conjugated linear polymers luminescent

Chemical Communications (2012)

Shoujun Zhu Junhu Zhang Lei Wang Yubin Song Guoyan Zhang

Photoluminescent polymer dots (PDs) were prepared by a moderate hydrothermal treatment of poly(vinyl alcohol) (PVA). A single excited state was established in the PL mechanism by ultrafast spectroscopy. Moreover, the applied method be used to prepare fluorescent polymer dots from other non-conjugated polymers, and shows general universality.

Vinyl alcohol

10.1039/c2cc36080b

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Citations (188)

Antimicrobial polyacrylic acid/tannic acid hydrogel wound dressing facilitating full-thickness skin healing

Journal of Biomaterials Science Polymer Edition (2024)

Changlin Zhou L. G. Chen Wenyan Zhou Lei Wang Ruitao Zhang

Polyphenolic compound-modified hydrogel wound dressings with excellent wet tissue adhesion, antimicrobial properties, stretchability, and full-thickness skin healing properties are still extremely rare so far. Polyphenolic compounds such as tannic acid or dopamine can improve the antibacterial and bioadhesive properties of hydrogels, and are also polymerization inhibitors for free radical polymerization. In this study, polyacrylic acid (PAA) aqueous solution was first synthesized, and then antibacterial PAA-TA hydrogel was prepared by mixing it with tannic acid (TA) and the crosslinker 1,6-hexanediol bis(2-methyl-1-propionic acid azide) (HBMAP). This method avoids the hindrance of the phenolic hydroxyl groups in TA on acrylic acid polymerization, and we were able to obtain a series of TA hydrogels (in the range of 0–15 wt.%. We applied these PAA-TA hydrogels to wound dressings and found that they had excellent adhesion to biological tissues, and the tensile strength and elongation at break of PAA-TA hydrogels with 15 wt.%TA content were as high as 1.72 MPa and 1446.3% in tensile strength evaluation. In addition, microbiological analysis showed that wound dressings had significant antimicrobial activity against Staphylococcus aureus and Escherichia coli. In vitro wound healing experiments confirmed that the wound dressing was biocompatible and could significantly promote the healing of full-thickness skin defects in the guinea pig model. Our work describes an injectable, self-healing, antimicrobial hydrogel that may have promising clinical applications as a wound dressing material.

Tannic acid

Polyacrylic acid

10.1080/09205063.2023.2300493

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Citations (2)

Engineering immunomodulatory and osteoinductive implant surfaces via mussel adhesion-mediated ion coordination and molecular clicking

Nature Communications (2022)

Tao Wang Jiaxiang Bai Min Lu Cheng-long Huang Dechun Geng

Abstract Immune response and new tissue formation are important aspects of tissue repair. However, only a single aspect is generally considered in previous biomedical interventions, and the synergistic effect is unclear. Here, a dual-effect coating with immobilized immunomodulatory metal ions (e.g., Zn 2+ ) and osteoinductive growth factors (e.g., BMP-2 peptide) is designed via mussel adhesion-mediated ion coordination and molecular clicking strategy. Compared to the bare TiO 2 group, Zn 2+ can increase M2 macrophage recruitment by up to 92.5% in vivo and upregulate the expression of M2 cytokine IL-10 by 84.5%; while the dual-effect of Zn 2+ and BMP-2 peptide can increase M2 macrophages recruitment by up to 124.7% in vivo and upregulate the expression of M2 cytokine IL-10 by 171%. These benefits eventually significantly enhance bone-implant mechanical fixation (203.3 N) and new bone ingrowth (82.1%) compared to the bare TiO 2 (98.6 N and 45.1%, respectively). Taken together, the dual-effect coating can be utilized to synergistically modulate the osteoimmune microenvironment at the bone-implant interface, enhancing bone regeneration for successful implantation.

10.1038/s41467-021-27816-1

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Citations (119)

Full-thickness osteochondral defect repair using a biodegradable bilayered scaffold of porous zinc and chondroitin sulfate hydrogel

Bioactive Materials (2023)

Fan Yang Yageng Li Lei Wang Haodong Che Xin Zhang

The regeneration of osteochondral tissue necessitates the re-establishment of a gradient owing to the unique characteristics and healing potential of the chondral and osseous phases. As the self-healing capacity of hyaline cartilage is limited, timely mechanical support during neo-cartilage formation is crucial to achieving optimal repair efficacy. In this study, we devised a biodegradable bilayered scaffold, comprising chondroitin sulfate (CS) hydrogel to regenerate chondral tissue and a porous pure zinc (Zn) scaffold for regeneration of the underlying bone as mechanical support for the cartilage layer. The photocured CS hydrogel possessed a compressive strength of 82 kPa, while the porous pure Zn scaffold exhibited a yield strength of 11 MPa and a stiffness of 0.8 GPa. Such mechanical properties are similar to values reported for cancellous bone. In vitro biological experiments demonstrated that the bilayered scaffold displayed favorable cytocompatibility and promoted chondrogenic and osteogenic differentiation of bone marrow stem cells. Upon implantation, the scaffold facilitated the simultaneous regeneration of bone and cartilage tissue in a porcine model, resulting in (i) a smoother cartilage surface, (ii) more hyaline-like cartilage, and (iii) a superior integration into the adjacent host tissue. Our bilayered scaffold exhibits significant potential for clinical application in osteochondral regeneration.

Hyaline cartilage

Chondrogenesis

Fibrocartilage

Cancellous bone

10.1016/j.bioactmat.2023.10.014

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Citations (31)

H₂S donor S‐propargyl‐cysteine for skin wound healing improvement via smart transdermal delivery

MedComm (2024)

Xiaoqing Zhao Chen Yao Zhongxiao Lin Jin Xinyang Bolun Su

Abstract Hydrogen sulfide for wound healing has drawn a lot of attention recently. In this research, the S‐propargyl‐cysteine (SPRC), an endogenous H 2 S donor, was loaded on carbomer hydrogel, and a copper sheet rat burn model was developed. Pathological changes in rat skin tissue were examined using hematoxylin–eosin (HE) and Masson staining. The immunohistochemistry (IHC) staining was performed to detect the expression of Collagen I (Col I) and Collagen III (Col III). The mRNA levels of interleukin (IL)‐6, Col Iα2, Col IIIα1, tissue inhibitors of metalloproteinase (TIMP)‐1, matrix metalloproteinase (MMP)‐9, vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)‐β1 were examined by quantitative real‐time chain polymerase reaction. The findings demonstrated that the collagen layer was thicker in the SPRC group during the proliferative phase, SPRC hydrogel promoted VEGF expression. In the late stage of wound healing, the expression of IL‐6, TIMP‐1, MMP‐9, and TGF‐β1 was inhibited, and the Col I content was closer to that of normal tissue. These results surface that SPRC hydrogel can promote wound healing and play a positive role in reducing scar formation. Our results imply that SPRC can facilitate wound healing and play a positive role in reducing scar formation.

10.1002/mco2.485

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Citations (2)

Chitooligosaccharides promote diabetic wound healing by mediating fibroblast proliferation and migration

Scientific Reports (2025)

Zihan Li Chuwei Zhang Lei Wang Qingrong Zhang Yipeng Dong

Diabetic wounds are notoriously difficult to heal due to impaired cell repair mechanisms, reduced angiogenesis, and a heightened risk of infection. Fibroblasts play a vital role in wound healing by producing extracellular matrix (ECM) components and various growth factors, but their function is inhibited in diabetic wounds. Chitooligosaccharides (COS), intermediate products of chitosan degradation, have shown efficacy in promoting tissue repair, yet their role in diabetic wound healing remains underexplored. In a mouse model of diabetic wounds, COS treatment demonstrated substantial bioactivity in accelerating wound healing by enhancing fibroblast proliferation and migration. Additionally, COS increased collagen III deposition and angiogenesis at the wound sites. The COS also mitigated inflammatory responses by controlling leukocyte infiltration and bacterial infection. Mechanistically, COS regulated fibroblast activity via the PI3K/Akt signaling pathway, providing a novel bioactive material for chronic wound healing.

10.1038/s41598-024-84398-w

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Citations (0)

Super-resolution analyzing spatial organization of lysosomes with an organic fluorescent probe

bioRxiv (Cold Spring Harbor Laboratory) (2022)

Lei Wang Rui Chen Guan‐Qun Han Xuan Liu Taosheng Huang

Abstract Lysosomes are multifunctional organelles involved in macromolecule degradation, nutrient sensing and autophagy. Live imaging has revealed lysosome subpopulations with dynamics and characteristic cellular localization. An as-yet unanswered question is whether lysosomes are spatially organized to coordinate and integrate their functions. Combined with super-resolution microscopy, we designed a small organic fluorescent probe, TPAE, that targeted lysosomes with a large Stokes shift. When we analyzed the spatial organization of lysosomes against mitochondria in different cell lines with this probe, we discovered different distance distribution patterns between lysosomes and mitochondria during increased autophagy flux. By using SLC25A46 mutation fibroblasts derived from patients containing highly fused mitochondria with low oxidative phosphorylation, we concluded that unhealthy mitochondria redistributed the subcellular localization of lysosomes, which implies a strong connection between mitochondria and lysosomes.

Organelle

10.1101/2022.02.14.480395

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Citations (3)

Machine Learning-based Start-up Company Lifespan Prediction: the Chinese Market as an Example

Lei Wang Tian-Ze Zhang Yingting Chen Yongyang Huang Xitong Yin

Start-ups have emerged as key drivers of economic growth, fostering innovation, job creation, and knowledge dissemination across various industries. Accurately forecasting start-up life spans is critical for investors, policymakers, and entrepreneurs to make informed decisions and optimize resource allocation. However, existing predictive models, such as linear regression and survival analysis, face challenges in capturing the complex interactions and dynamic nature of factors influencing start-up success. This paper proposes applying the XGBoost algorithm, an advanced machine learning technique, to enhance the accuracy and reliability of start-up life span predictions. XGBoost offers several advantages over traditional methods, including adaptability to various data types, robustness to outliers, and efficient computational performance. By incorporating a wide range of features, such as financial, organizational, and death reasons, the algorithm can effectively capture the complex relationships among these factors without explicit feature engineering. Moreover, applying SHAP values provides an additional layer of interpretability, aiding stakeholders in better understanding the factors driving start-up life span. Utilizing the IT Orange dataset, we investigate the determinants of startup life spans, offering valuable insights for stakeholders in the entrepreneurial ecosystem.

Interpretability

Robustness

Adaptability

Feature Engineering

10.1109/uv56588.2022.10185504

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Citations (1)