Kang Zhang

Institute of Vegetables and Flowers

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Feng Cheng

Chinese Academy of Agricultural Sciences

Jingyan Zhang

Lanzhou Institute of Husbandry and Pharmaceutical Sciences

Jianxi Li

Chinese Academy of Agricultural Sciences

Zhenglin Yang

University of Electronic Science and Technology of China

Wenxian Sun

China Agricultural University

Lingkui Zhang

Ministry of Agriculture and Rural Affairs

Xiaobing Fu

Chinese PLA General Hospital

Shumin Chen

Ministry of Agriculture and Rural Affairs

Xiaolei Li

Soochow University

Weidong Han

Zhejiang Cancer Hospital

Cooperative Institutions

Chinese Academy of Agricultural Sciences

196

Ministry of Agriculture and Rural Affairs

161

Chinese Academy of Sciences

145

Sichuan University

101

Institute of Vegetables and Flowers

Zhejiang University

Johns Hopkins University

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Hebei Agricultural University

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Antiviral activity and underlying mechanisms of baicalin against avian infectious bronchitis virus in vitro

Avian Pathology (2022)

Haipeng Feng Kai Zhang Kang Zhang Zhiting Guo Qin Liu

Baicalin, a flavonoid compound extracted from the dry root of Scutellaria baicalensis Georgi, has been shown to have anti-inflammation, anti-viral, anti-bacterial, and immunomodulatory activity. However, the effect of baicalin against avian infectious bronchitis virus (IBV) remains unknown. The purpose of this study was to investigate the anti-IBV activity and underlying mechanism of baicalin in vitro. The results showed that baicalin has a direct virucidal effect but no prophylactic effect on IBV infection. The mRNA and protein of IBV N were decreased significantly when IBV-infected cells were treated with baicalin during the multiple stages of the virus replication cycle, including viral adsorption, invasion, internalization, and release. Stress granule (SG) formation resulted from the increase of G3BP1 and the phosphorylation of the PKR/eIF2α due to the treatment of IBV-infected cells with baicalin. The inhibitory activity of baicalin on IBV replication was increased when G3BP1 expression was inhibited, and the down-regulation of G3BP1 expression occurred when the expression of PKR and eIF2α was inhibited. These findings revealed that baicalin activates phosphorylation of the PKR/eIF2α pathway and induces SG formation by targeting G3BP1, initiating the antiviral response to suppress IBV replication in Vero cells. The results suggest that baicalin is a promising candidate drug to treat or prevent IBV infection.RESEARCH HIGHLIGHTS Baicalin inhibits IBV replication by reducing IBV N protein and mRNA.Baicalin disturbs multiple stages of the IBV life cycle.Baicalin activates PKR/eIF2α pathway and induces stress granule formation to exert anti-IBV response.

Baicalin

Stress granule

10.1080/03079457.2022.2109453

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Citations (11)

Peer Review #2 of "Transcriptomic and metabolomic analysis of autumn leaf color change in Fraxinus angustifolia (v0.2)"

Yanlong Wang Jinpeng Zhen Xiaoyu Che Kang Zhang Guowei Zhang

Fraxinus angustifolia is a type of street tree and shade tree with ornamental value.It has a beautiful shape and yellow or reddish purple autumn leaves, but its leaf color formation mechanism and molecular regulation network need to be studied.In this study, we integrated the metabolomes and transcriptomes of stage 1 (green leaf) and stage 2 (redpurple leaf) leaves at two different developmental stages to screen differential candidate genes and metabolites related to leaf color variation.The results of stage 1 and stage 2 transcriptome analysis showed that a total of 5827 genes were differentially expressed, including 2249 upregulated genes and 3578 downregulated genes.Through functional enrichment analysis of differentially expressed genes, we found that they were involved in flavonoid biosynthesis, phenylpropanoid biosynthesis, pigment metabolism, carotene metabolism, terpenoid biosynthesis, secondary metabolite biosynthesis, pigment accumulation, and other biological processes.By measuring the metabolites of Fraxinus angustifolia leaves, we found the metabolites closely related to the differentially expressed genes in two different periods of Fraxinus angustifolia, among which flavonoid compounds were the main differential metabolites.Through transcriptome and metabolomics data association analysis, we screened nine differentially expressed genes related to anthocyanins.Transcriptome and qRT-PCR results showed that these nine genes showed significant expression differences in different stages of the sample, and we speculate that they are likely to be the main regulatory factors in the molecular mechanism of leaf coloration.This is the first time that we have analyzed the transcriptome combination metabolome in the process of leaf coloration of Fraxinus angustifolia, which has important

10.7287/peerj.15319v0.2/reviews/2

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Towards understanding the biosynthetic pathway for ustilaginoidin mycotoxins in Ustilaginoidea virens

Environmental Microbiology (2019)

Yuejiao Li Ming Wang Zhaohui Liu Kang Zhang Fuhao Cui

Summary Ustilaginoidins, toxic to plants, animals and human, are one of major types of mycotoxins produced by Ustilaginoidea virens . In this study, a gene cluster containing the polyketide synthase gene UvPKS1 was analysed via gene replacement and biochemical studies to determine ustilaginoidin biosynthetic pathway in U. virens . UvPKS1 was first proven to be responsible for the first step of ustilaginoidin biosynthesis, since neither ustilaginoidin derivatives nor intermediates were produced when UvPKS1 was deleted. Replacement of ugsO greatly reduced ustilaginoidin production but increased the ratios of dehydrogenated/hydrogenated ustilagioidin derivatives. The enhanced growth rate of the Δ ugsO mutant indicates that accumulation of certain ustilaginoidin derivatives may adversely affect mycelial growth in U. virens . Deletion of ugsT encoding a putative MFS transporter disrupted the ability to generate ustilaginoidins. The ustilaginoidin derivatives produced in the Δ ugsJ mutant all lack C3‐methyl, indicating that UgsJ is responsible for C3‐methylation. Only monomeric intermediates, such as 3‐methyl‐dihydro‐nor‐rubrofusarin, but no ustilaginoidin derivatives were generated in the Δ ugsL mutant, indicating that UgsL is responsible for the dimerization of nor‐rubrofusarin derivatives to produce ustilaginoidins. However, ugsR2 deletion had no dramatic effect on ustilaginoidin biosynthesis. Together, biochemical analyses with bioinformatics and chemoinformatics uncover a multiple‐step enzyme‐catalysed pathway for ustilaginoidin biosynthesis in U. virens .

Polyketide synthase

Gene cluster

10.1111/1462-2920.14572

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Citations (45)

Cordyceps militaris Solid Medium Extract Alleviates Lipoteichoic Acid-Induced MH-S Inflammation by Inhibiting TLR2/NF-κB/NLRP3 Pathways

International Journal of Molecular Sciences (2023)

Xiaoya Wang Zhiping Peng Lei Wang Jingyan Zhang Kang Zhang

The aim of this study was to investigate the inhibitory effects of Cordyceps militaris solid medium extract (CME) and cordycepin (COR) on LTA-induced inflammation in MH-S cells and their mechanisms of action. In this study, the establishment of an LTA-induced MH-S inflammation model was determined, the CCK-8 method was used to determine the safe concentration range for a drug for COR and CME, the optimal concentration of COR and CME to exert anti-inflammatory effects was further selected, and the expression of inflammatory factors of TNF-α, IL-1β, IL-18, and IL-6 was detected using ELISA. The relative expression of TNF-α, IL-1β, IL-18, IL-6, IL-10, TLR2 and MyD88 mRNA was detected using RT-PCR, and the IL-1β, IL-18, TLR2, MyD88, NF-κB p-p65, NLRP3, pro-caspase-1, Caspase-1 and ASC protein expression in the cells were detected using Western blot; immunofluorescence assay detected the expression of Caspase-1 in MH-S cells. The results revealed that both CME and COR inhibited the levels of IL-1β, IL-18, IL-6, and TNF-α in the supernatants of LTA-induced MH-S cells and the mRNA expression levels of IL-1β, IL-18, IL-6, TNF-α, TLR2 and MyD88, down-regulated the LTA-induced IL-1β, IL-18, TLR2 in MH-S cells, MyD88, NF-κB p-p65/p65, NLRP3, ASC, pro-caspase-1, and caspase-1 protein expression levels, and inhibited LTA-induced caspase-1 activation in MH-S cells. In conclusion, CME can play a therapeutic role in LTA-induced inflammation in MH-S cells via TLR2/NF-κB/NLRP3, and may serve as a potential drug for bacterial pneumonia caused by Gram-positive bacteria.

Lipoteichoic acid

Cordycepin

10.3390/ijms242115519

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ZmMYC7 directly regulates ZmERF147 to increase maize resistance to Fusarium graminearum

The Crop Journal (2022)

Hongzhe Cao Kang Zhang Wei Li Xi Pang Pengfei Liu

The jasmonic acid (JA) signaling pathway is involved in plant growth, development, and response to abiotic or biotic stresses. MYC2, a bHLH transcription factor, is a regulatory hub in the pathway. The function of ZmMYC7, a putative MYC2 ortholog, in jasmonate-signaled defense responses of maize has not been reported. In this study, we found that ZmMYC7 possesses JID, TAD, bHLH and Zip domains and essential characteristics of transcription factors: a nuclear location and transactivation activity. The ZmMYC7 mutants showed markedly increased sensitivity to Fusarium graminearum and Setosphaeria turcica. The expression levels of the defense-associated genes ZmPR1, ZmPR2, ZmPR3, ZmPR5, ZmPR6, and ZmPR7 in response to F. graminearum infection were downregulated in ZmMYC7 mutants, while ZmPR4 and ZmPR10 were up-regulated. ZmMYC7 interacted with members of the ZmJAZ family, including ZmJAZ8, ZmJAZ11, and ZmJAZ12. ZmMYC7 physically interacted with G-box cis-elements in the ZmERF147 promoter in vitro and transcriptional activation of ZmERF147 by ZmMYC7 was inhibited by ZmJAZ11 and ZmJAZ12. ZmERF147 mutants were more susceptible to F. graminearum infection than inbred line B73 with concomitant down-regulation of all defense-associated ZmPRs except ZmPR4. These findings indicate that ZmMYC7 functions in maize resistance to F. graminearum and sheds light on maize defense responses to pathogenic fungi via the JA signaling pathway.

Jasmonic acid

Jasmonate

10.1016/j.cj.2022.05.006

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Identification and optimization of genes potentially related to protein expression for enhancing α-amylase production in Bacillus subtilis

Systems Microbiology and Biomanufacturing (2024)

Hui Luo Xuyang Zhu Jing Wu Kang Zhang

Identification

10.1007/s43393-024-00234-2

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Citations (3)

Co-expression network analyses identify functional modules associated with development and stress response in Gossypium arboreum

Scientific Reports (2016)

Qi You Liwei Zhang Xin Yi Kang Zhang Dongxia Yao

Abstract Cotton is an economically important crop, essential for the agriculture and textile industries. Through integrating transcriptomic data, we discovered that multi-dimensional co-expression network analysis was powerful for predicting cotton gene functions and functional modules. Here, the recently available transcriptomic data on Gossypium arboreum , including data on multiple growth stages of tissues and stress treatment samples were applied to construct a co-expression network exploring multi-dimensional expression (development and stress) through multi-layered approaches. Based on differential gene expression and network analysis, a fibre development regulatory module of the gene GaKNL1 was found to regulate the second cell wall through repressing the activity of REVOLUTA , and a tissue-selective module of GaJAZ1a was examined in response to water stress. Moreover, comparative genomics analysis of the JAZ1 -related regulatory module revealed high conservation across plant species. In addition, 1155 functional modules were identified through integrating the co-expression network, module classification and function enrichment tools, which cover functions such as metabolism, stress responses, and transcriptional regulation. In the end, an online platform was built for network analysis ( http://structuralbiology.cau.edu.cn/arboreum ), which could help to refine the annotation of cotton gene function and establish a data mining system to identify functional genes or modules with important agronomic traits.

Gossypium

Functional Genomics

Gene regulatory network

Gene co-expression network

Network Analysis

10.1038/srep38436

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Citations (36)

Xenobiotic Pregnane X Receptor (PXR) Regulates Innate Immunity via Activation of NLRP3 Inflammasome in Vascular Endothelial Cells

Journal of Biological Chemistry (2014)

Shaolan Wang Ting Lei Kang Zhang Wenxiang Zhao Fang Li

Pregnane X receptor

10.1074/jbc.m114.578781

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Citations (49)

Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice

Journal of Clinical Investigation (2009)

Zhenglin Yang Yali Chen Concepción Lillo Jeremy Chien Zhengya Yu

During the preparation of the manuscript, the mice in Figure 2 were incorrectly identified as having been infected with S. mansoni.The correct legend for Figure 2A appears below.(A) Mice received 1 or more egg injections.Repeat egg injections were 5 d apart, such that mice for the 20-d time point had received 4 injections prior to sacrifice.At 5, 10, 15, and 20 d after the first immunization, CD4 + T cells from draining popliteal LNs were analyzed ex vivo for the expression of IL-4/GFP by flow cytometry.Numbers within plots indicate mean ± SD percent of CD4 + cells that were IL-4/GFP + (3 mice per group).The JCI regrets the error.

Degeneration (medical)

10.1172/jci35891c1

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Citations (22)

Evaluation of a hybrid automatic planning solution for rectal cancer

Radiation Oncology (2022)

Jiyou Peng Yu Lei Fan Xia Kang Zhang Zhen Zhang

Abstract Background Script-based planning and knowledge-based planning are two kinds of automatic planning solutions. Hybrid automatic planning may integrate the advantages of both solutions and provide a more robust automatic planning solution in the clinic. In this study, we evaluated and compared a commercially available hybrid planning solution with manual planning and script-based planning. Methods In total, 51 rectal cancer patients in our institution were enrolled in this study. Each patient generated 7 plans: one clinically accepted manual plan ( $${plan}_{manual}$$ plan manual ), three script-based plans and three hybrid plans generated with the volumetric-modulated arc therapy technique and 3 different clinical goal settings: easy, moderate and hard ( $${plan}_{hybrid}^{easy}$$ plan hybrid easy , $${plan}_{hybrid}^{moderate}$$ plan hybrid moderate , $${plan}_{hybrid}^{hard}$$ plan hybrid hard , $${plan}_{script}^{easy}$$ plan script easy , $${plan}_{script}^{moderate}$$ plan script moderate and $${plan}_{script}^{hard}$$ plan script hard ). Planning goals included planning target volume (PTV) D max , bladder D mean and femur head D mean . The PTV prescription was the same (50.00 Gy) for the 3 goal settings. The hard setting required a lower PTV D max and stricter organ at risk (OAR) dose, while the easy setting was the opposite. Plans were compared using dose metrics and plan quality metric (PQM) scores, including bladder D 15 and D 50 , left and right femur head D 25 and D 40 , PTV D 2 , D 98 , CI (conformity index) and HI (homogeneity index). Results Compared to manual planning, hybrid planning with all settings significantly reduced the OAR dose ( p < 0.05, paired t-test or Wilcoxon signed rank test) for all dose-volume indices, except D 25 of the left femur head. For script-based planning, $${plan}_{script}^{easy}$$ plan script easy significantly increased the OAR dose for the femur head and D 2 and the PTV homogeneity index ( p < 0.05, paired t-test or Wilcoxon signed rank test). Meanwhile, the maximum dose of the PTV was largely increased with hard script-based planning (D2 = 56.06 ± 7.57 Gy). For all three settings, the comparison of PQM between hybrid planning and script-based planning showed significant differences, except for D 25 of the left femur head and PTV D 2 . The total PQM showed that hybrid planning could provide a better and more robust plan quality than script-based planning. Conclusions The hybrid planning solution was manual-planning comparable for rectal cancer. Hybrid planning can provide a better and more robust plan quality than script-based planning.

10.1186/s13014-022-02129-9

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Citations (2)