Feng Wang

Zunyi Medical University

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Juewen Liu

University of Waterloo

Jun Wang

Northwestern Polytechnical University

Ning Zhao

Bohai University

Fukui Xiao

Institute of Coal Chemistry

Chao Li

Changchun Normal University

Yucai Wang

University of Science and Technology of China

Yuhan Sun

ShanghaiTech University

Yong Yan

Henan Normal University

Liping Sun

Shanghai Tenth People's Hospital

Biwu Liu

Xi'an Jiaotong University

Cooperative Institutions

Chinese Academy of Sciences

182

University of Science and Technology of China

131

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Sun Yat-sen University

Hefei University of Technology

Huazhong University of Science and Technology

Central South University

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

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Copolypeptide-doped polyaniline nanofibers for electrochemical detection of ultratrace trinitrotoluene

Talanta (2009)

Feng Wang Wenbo Wang Bianhua Liu Zhenyang Wang Zhongping Zhang

Polyaniline nanofibers

Trinitrotoluene

10.1016/j.talanta.2009.03.062

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Citations (52)

Fluidity-Guided Assembly of Au@Pt on Liposomes as a Catalase-Powered Nanomotor for Effective Cell Uptake in Cancer Cells and Plant Leaves

ACS Nano (2022)

Zhenfeng Wang Yong Yan Chao Li Yue Yu Sheng Cheng

The fluidity of the liposomes is essential to nanoparticle–membrane interactions. We herein report a liposomal nanomotor system by controlling the self-assembly behavior of gold core–platinum shell nanoparticles (Au@Pt) on liposomes. Au@Pt can aggregate immediately on fluid-phase dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes, forming an uneven distribution. By control of the lipid phase and fluidity, either using pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) above its phase transition temperature or adding cholesterol as an adjuvant to DPPC lipids, we precisely control the assembly of Au@Pt on liposomes. Au@Pt maintained high catalase-like activity on the liposomal surface, promoting the decomposition of H2O2 and the movement of the liposomal nanomotors. Finally, we demonstrate that liposomal nanomotors are biocompatible and they can speed up the cellular uptake in mammalian HepG2 cancer cells and Nicotiana tabacum (Nb) plant leaves. This liposomal nanomotor system is expected to be further investigated in biomedicine and plant nanotechnology.

10.1021/acsnano.2c00327

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Citations (30)

Enhancement of lipopolysaccharide-induced nitric oxide and interleukin-6 production by PEGylated gold nanoparticles in RAW264.7 cells

Nanoscale (2012)

Zhimin Liu Wenqing Li Feng Wang Chunyang Sun Lu Wang

While the immunogenicity and cytotoxicity of gold nanoparticles (AuNPs) are noted by many researchers, the mechanisms by which AuNPs exert these effects are poorly understood. In this study, we investigated the effects of polyethylene glycolylated AuNPs (PEG@AuNPs) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and interleukin-6 (IL-6) production and the associated molecular mechanism in RAW264.7 cells. The results showed that PEG@AuNPs were internalized more quickly by LPS-activated RAW264.7 cells than unstimulated cells, and they reached saturation within 24 hours. PEG@AuNPs enhanced LPS-induced production of NO and IL-6 and inducible nitric oxide synthase (iNOS) expression in RAW264.7 cells, partially by activating p38 mitogen-activated protein kinases (p38 MAPK) and nuclear factor-kappaB pathways. In addition, the p38 MAPK inhibitor SB203580 attenuated PEG@AuNP-enhanced LPS-induced NO production and iNOS expression. Overproduction of NO and IL-6 is known to be closely correlated with the pathology of many diseases and inflammations. Thus, it is speculated that the highly biocompatible gold nanoparticles can induce immunotoxicity due to their potency to stimulate macrophages to release aberrant or excessive pro-inflammatory mediators.

10.1039/c2nr31355c

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Citations (56)

The hybrid polyhydroxyurethane materials synthesized by a prepolymerization method from CO2-sourced monomer and epoxy

Journal of CO2 Utilization (2016)

Jiexi Ke Xiaoyun Li Feng Wang Maoqing Kang Yuelan Feng

Diglycidyl ether

Thermogravimetric analysis

Polypropylene

10.1016/j.jcou.2016.11.001

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Citations (33)

Charge and Coordination Directed Liposome Fusion onto SiO₂ and TiO₂ Nanoparticles

Langmuir (2018)

Xiaoshun Wang Xiaoqiu Li Hui Wang Xiaohan Zhang Lei Zhang

TiO2 and SiO2 are very useful materials for building biointerfaces. A particularly interesting aspect is their interaction with lipid bilayers. Many past research efforts focused on phosphocholine (PC) lipids, which form supported lipid bilayers (SLB) on SiO2 at physiological conditions but are adsorbed as intact liposomes on TiO2. Low pH was required to form PC SLBs on TiO2. This work intends to understand the surface forces and chemistry responsible for such differences. Two charge neutral lipids: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 2-((2,3-bis(oleoyloxy)propyl)dimethylammonio)ethyl ethyl phosphate (DOCPe) and two negatively charged lipids: 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) and 2-((2,3-bis(oleoyloxy)propyl)dimethylammonio)ethyl hydrogen phosphate (DOCP) were used. Using calcein leakage assays, adsorption measurement, cryo-TEM, and washing, we concluded that charge is the dominating factor on SiO2. The two neutral lipids form SLB on SiO2 at pH 3 and 7, but the two negatively charged ones cannot form. On TiO2, both charge and coordination chemistry are important. The two anionic lipids formed SLB from pH 3 to 10. DOCP had stronger affinity than DOPS likely due to the tighter terminal phosphate binding of the former. The two neutral liposomes formed SLB only at pH 3, where phosphate interaction and van der Waals force are deemed important. The pH 3 prepared TiO2 DOPC SLBs are destabilized at neutral pH, indicating the reversible nature of the interaction. This work has provided new insights into two important materials interacting with common liposomes, which are important for reproducible biosensing, device fabrication, and drug delivery applications.

Calcein

Surface charge

10.1021/acs.langmuir.8b02979

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Citations (22)

Effect of Gambogic Acid–Loaded Porous-Lipid/PLGA Microbubbles in Combination With Ultrasound-Triggered Microbubble Destruction on Human Glioma

Frontiers in Bioengineering and Biotechnology (2021)

Feng Wang Lei Dong Xixi Wei Yongling Wang Liansheng Chang

Gambogic acid (GA) is a highly effective antitumor agent, and it is used for the treatment of a wide range of cancers. It is challenging to deliver drugs to the central nervous system due to the inability of GA to cross the blood–brain barrier (BBB). Studies have shown that ultrasound-targeted microbubble destruction can be used for transient and reversible BBB disruption, significantly facilitating intracerebral drug delivery. We first prepared GA–loaded porous-lipid microbubbles (GA porous-lipid/PLGA MBs), and an in vitro BBB model was established. The cell viability was detected by CCK-8 assay and flow cytometry. The results indicate that U251 human glioma cells were killed by focused ultrasound (FUS) combined with GA/PLGA microbubbles. FUS combined with GA/PLGA microbubbles was capable of locally and transiently enhancing the permeability of BBB under certain conditions. This conformational change allows the release of GA to extracellular space. This study provides novel targets for the treatment of glioma.

Gambogic acid

PLGA

Viability assay

10.3389/fbioe.2021.711787

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Citations (7)

Ion-Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine

Methods in molecular biology (2016)

Richard R. Rustandi Feng Wang Catherine Lancaster Adam Kristopeit David S. Thiriot

Ion chromatography

10.1007/978-1-4939-6361-4_20

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Citations (5)

Evaluation of the Automated Indirect Immunofluorescence Test for Antinuclear Antibodies.

Annals of clinical and laboratory science (2020)

Shiji Wu Feng Liu Feng Wang Jin Huang Botao Yin

The performance of the automated indirect immunofluorescence system was compared with the manual method for detection of antinuclear antibodies (ANA) from 354 clinical serum samples. We compared the results (negative or positive), ANA patterns, and titers for the two methods. The coincidence rates for ANA positive and negative samples were 93.4% and 98.7%, respectively. The coincidence rates for single patterns, mixed patterns, and the final titers were 85.1%, 87.6%, and 87.6%, respectively. The homogeneous, speckled, cytoplasmic, centromere, multiple nuclear dots, and nucleolar pattern coincidence rates were 79.3%, 83.0%, 87.8%, 72.7%, 50%, and 56.3%, respectively. The automated indirect immunofluorescence system had acceptable accuracy for detection of ANA.

Immunofluorescence

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Citations (2)

The agglutination of the crude proteins from 4 species of animals

Journal of Qiqihar University (2004)

Feng Wang

8native erythrocytes from 5 kinds of vertebrates,7 kinds of single-cell microbe and 5 single-cell algae were used to detect agglutination activity from 4 marine mollusceas. All protein solutions have hemagglutination. Sheep erythrocyte, Saccharomyces carlsbergensi and Porphyridium purpureum are more sensitive to agglutination than the others. The agglutinations need pH 6.5～8.0 as fit conditions, but can’t tolerate high temperature. They all lost the activity under 60℃ or 65℃ for 15min .The hemagglutination activity can be inhibited by different kinds of saccharide and 0.02mol/L EDTA.

Agglutination (biology)

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Direct measurement of sequence-dependent transition path times and conformational diffusion in DNA duplex formation

Proceedings of the National Academy of Sciences (2017)

Krishna Neupane Feng Wang Michael T. Woodside

Significance The transition path time in folding reactions—the time required to cross through the high-energy transition states—offers a powerful tool for studying folding mechanisms because it is uniquely sensitive to the microscopic motions along the transition paths that dominate the folding dynamics. We used transition path times to probe helix formation in nucleic acids and elucidate differences between the formation of canonical G:C and A:T base pairs. Stem-loop hairpins of the same size but increased G:C content had faster transition path times, revealing that the conformational diffusion coefficient determining the timescale for molecular dynamics was higher for formation of G:C base pairs than A:T base pairs. Such sequence dependence, not previously observed, reflects differences in the internal friction for reconfiguring the bases.

Folding (DSP implementation)

Duplex (building)

Sequence (biology)

Helix (gastropod)

Transition state

10.1073/pnas.1611602114

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Citations (49)