Different concentrations of exogenous hormones were applied on rapeseed at the initial blossoming stage.The net photosynthetic rate (P n ) and yield were investigated.In January 2008, a heavy snow and low temperature for a period destroyed crops including winter rape.In this experiments, exogenous hormones ABA and BR with optimal concentrations were applied on the rapeseed (Brassica napus L.) plants after the frozen from the worst weather.The gas exchange parameters and chlorophyll fluorescence characteristics of leaves as well as the regularity of pod size changes and yield components were determined.The results were as follows: ABA (76×10 -6 mol L -1 )and BR(1×10 -6 mol L -1 ) could delay the decreasing of net photosynthetic rate (P n ) in short petiole leaves.The P n , stomatal conductance (G s ), intercellular CO 2 concentration (C i ), transpiration rate (T r ) and photosynthetic saturated light intensity (LSP) were increased after exogenous hormones treatment.ABA and BR treatments also caused a significant increase in the maximum photochemical efficiency of photosystem II (F v /F m ), the quantum yield of electron transport (Φ PSII ), the photochemical quenching co-efficient (q P ), Chl content and soluble protein content.These changes were accompanied by the decline in both minimal fluorescence (F o ) and non-photochemical quenching co-efficient (NPQ).The pod surface areas per pod were increased.The number of the first branches per plant and seeds per pod and 1000-seed weight were raised, resulting in improvement of yield after BR treatment.As to the plants after ABA treatment, the high yield was mainly attributed to the increasing of seeds per pod and 1000-seed weight.In conclusion, ABA (76×10 -6 mol L -1 ) and BR (1×10 -6 mol L -1 ) treatments are effective on promoting the growth and relieving the negative effects of frozen on rapeseed.
The aim of the present study was to identify and characterize polymorphisms within the coding region and the 3' flanking region of the bovine serotonin receptor 1B gene among different cattle breeds. Four DNA fragments were amplified by polymerase chain reaction and then used for polymorphism identification by SSCP. The fragments showing different SSCP patterns were sequenced. And a total of six SNPs (G205T, C507T, C546G, C744T, G816A and G942A) were detected. The SNPs were at Hardy-Weinberg equilibrium except C507T and C546G in all genetic population. The frequencies of allele 205T of Holstein were much higher than that of the other six beef cattle populations. Almost the PIC of all SNPs were not more than 0.10 except that of G205T in Holstein cows, which indicated the bovine HTR1B gene was conserved.
contaminative fungal isolates were obtained from wooden cultures of Tremella fuciformis which were collected from four different geographic origins. Morphologic identification showed that the 48 tested strains belonged to Annulohypoxylon and bore
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