It has been reported that bone marrow-derived mesenchymal stem cells (BMSCs) have capacity to migrate to the damaged liver and contribute to fibrogenesis in chronic liver diseases. 15-Deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ 2 ), an endogenous ligand for peroxisome proliferator-activated receptor gamma (PPAR γ ), is considered a new inhibitor of cell migration. However, the actions of 15d-PGJ 2 on BMSC migration remain unknown. In this study, we investigated the effects of 15d-PGJ 2 on the migration of BMSCs using a mouse model of chronic liver fibrosis and primary mouse BMSCs. Our results demonstrated that in vivo , 15d-PGJ 2 administration inhibited the homing of BMSCs to injured liver by flow cytometric analysis and, in vitro , 15d-PGJ 2 suppressed primary BMSC migration in a dose-dependent manner determined by Boyden chamber assay. Furthermore, the repressive effect of 15d-PGJ 2 was blocked by reactive oxygen species (ROS) inhibitor, but not PPAR γ antagonist, and action of 15d-PGJ 2 was not reproduced by PPAR γ synthetic ligands. In addition, 15d-PGJ 2 triggered a significant ROS production and cytoskeletal remodeling in BMSCs. In conclusion, our results suggest that 15d-PGJ 2 plays a crucial role in homing of BMSCs to the injured liver dependent on ROS production, independently of PPAR γ , which may represent a new strategy in the treatment of liver fibrosis.
The original version of this article contained an error in the spelling of the author Yuchen Chen, which was incorrectly given as Yuhuan Chen. This has now been corrected in both the PDF and HTML versions of the article.
Using photothermal therapy to treat cancer has become an effective method, and the design of photothermal agents determines their performance. However, due to the major radiative recombination of a photogenerated electron in photothermal materials, the photothermal performance is weak which hinders their applications. In order to solve this issue, preventing radiative recombination and accelerating nonradiative recombination, which can generate heat, has been proved as a reasonable way. We demonstrated a Cu 2 MoS 4 @MXene nanocomposite with an obviously enhanced photothermal conversion efficiency ( η = 87.98%), and this improvement can be attributed to the electron migration. Then, a mechanism is proposed based on the electron transfer regulatory effect and the localized surface plasmon resonance effect, which synergistically promote nonradiative recombination and generate more heat. Overall, our design strategy shows a way to improve the photothermal performance of Cu 2 MoS 4 , and this method can be extended to other photothermal agents to let them be more efficient in treating cancer.
Islet β-cell dysfunction is a basic pathophysiological characteristic of type 2 diabetes mellitus (T2DM). Appropriate assessment of islet β-cell function is beneficial to better management of T2DM. Protecting islet β-cell function is vital to delay the progress of type 2 diabetes mellitus. Therefore, the Pancreatic Islet β-cell Expert Panel of the Chinese Diabetes Society and Endocrinology Society of Jiangsu Medical Association organized experts to draft the "Clinical expert consensus on the assessment and protection of pancreatic islet β-cell function in type 2 diabetes mellitus." This consensus suggests that β-cell function can be clinically assessed using blood glucose-based methods or methods that combine blood glucose and endogenous insulin or C-peptide levels. Some measures, including weight loss and early and sustained euglycemia control, could effectively protect islet β-cell function, and some newly developed drugs, such as Sodium-glucose cotransporter-2 inhibitor and Glucagon-like peptide-1 receptor agonists, could improve islet β-cell function, independent of glycemic control.
Nowadays, due to the excessive dependence on chemical fertilizers and pesticides in agricultural production, many problems, such as soil hardening and soil-borne diseases, have become increasingly prominent, which seriously restrict the sustainable development of agriculture. The application of microbial fertilizer prepared by biocontrol microorganisms can not only improve soil structure and increase fertility but also have the function of controlling diseases. Streptomyces aureoverticillatus HN6 has obvious disease prevention and growth promotive effect, which can improve the rhizosphere fertility of plants and even regulate the rhizosphere microbial community of plants. Based on the comparison of frame composting and natural composting, we used the response surface method to optimize the preparation conditions of Streptomyces HN6 bacterial fertilizer. The results showed that natural composting not only produced higher composting temperatures and maintained long high temperature periods in accordance with local conditions, but was also more suitable for composting in the field according to local conditions. Therefore, the substrate's conductivity changed more, the ash accumulation increased, and the substrate decomposed more thoroughly. Thus, this composting method is highly recommended. Additionally, Streptomyces HN6 microbial fertilizer EC20 can reduce cowpea fusarium wilt and promote cowpea growth. The number of plant leaves, plant height and fresh weight, increased significantly in the microbial fertilizer EC20. Moreover, Streptomyces HN6 fertilizer EC20 could significantly induce soil invertase, urease and catalase activities. Our study highlights the potential use of Streptomyces HN6 as a biofertilizer to improve plant productivity and biological control of plant pathogenic fungi.
The unbalanced tension and conductor jump caused by ice-shedding from overhead line may lead to tower collapse and transmission line discharge in serious cases, which seriously threatens the safe operation of the power grid. In response to the problem of insufficient research on the prediction for response characteristics parameters of isolated-span transmission lines under various ice-shedding modes, study on the prediction for ice-shedding response parameters of isolated-span overhead lines in southern China is conducted in this paper based on finite element simulation and machine learning. Firstly, the finite element simulation model of isolated-span overhead line is established and verified. Seven model input parameters are determined by considering 12 typical ice-shedding modes and ice-shedding labels are introduced. Six key output parameters reflecting the electrical and mechanical characteristics of the ice-shedding transmission line are obtained by feature extraction. Secondly, the output parameters of the ice-shedding transmission line under different input parameter conditions are obtained by using the finite element simulation method. A total of 1400 sets sample data are constructed. Then, four typical regression prediction models of BP, RBF, SVM and RF are established to predict the key output parameters of ice-shedding transmission lines. Finally, the optimal prediction model for ice-shedding ......
Dopamine (DA), a kind of neurotransmitter in animal, has been proved to cause a positive influence on plant during abiotic stress. In the present study, the function of DA on plants under cadmium (Cd) stress was revealed. The endogenous DA content decreased by 17% in duckweed by Cd stress. Exogenous DA supplement improved Cd resistance by reducing root abscission as well as restoring photosynthesis under Cd stress. Also, 24 h DA treatment increased Cd content by 1.3 times in duckweed under Cd stress, which was caused by promoting the influx of Cd. Besides, the gene expression changes study showed that photosynthesis related genes were up-regulated by DA addition with Cd stress. Additionally, the mechanism of DA-induced Cd detoxification and accumulation were also investigated, some critical gene such as VIT1 and Rubisco were significantly up-regulated with DA addition under Cd stress. An increase in intracellular Ca 2+ content and a decrease in Ca 2+ efflux induced by DA synchrony with changes in CNGC2 expression were observed, predicting that CNGC2 may be an upstream site for DA action. Our results demonstrate that DA addition alleviated Cd stress by an increased photosynthesis system, an enhanced Cd detoxification and accumulation, and an increase in intracellular Ca 2+ content in duckweed during Cd stress. The function of DA in Cd stress offers a new insight for the application and study of DA to Cd phytoremediation of aquatic systems.
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