Yi Li

University of Connecticut

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Richard McAvoy

University of Connecticut

Degang Zhao

Guizhou Academy of Agricultural Sciences

Yongqin Chen

Union Hospital

Hui Duan

U.S. National Arboretum

Tingting Gu

Nanjing Agricultural University

Xueju Wang

University of Connecticut

Chandra S. Thammina

Washington State University

Huseyin Yer

University of Connecticut

Yan Pei

Southwest University

Lorenzo Katin‐Grazzini

University of Connecticut

Cooperative Institutions

University of Connecticut

Nanjing Agricultural University

Chinese Academy of Sciences

China Agricultural University

Ministry of Agriculture and Rural Affairs

Sun Yat-sen University

University of Chinese Academy of Sciences

Sichuan University

Huazhong University of Science and Technology

Central South University

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The GLP‐1 receptor agonist liraglutide protects against oxidized LDL‐induced endothelial inflammation and dysfunction via KLF2

IUBMB Life (2019)

Yue Wen Yi Li Dengke Ou Qing Yang

Cardiovascular complications are the major causes of the mortality and morbidities in diabetic patients. The diabetic patients have an increased risk of developing atherosclerosis, which could lead to heart attack and stroke. Glucagon-like peptide 1 (GLP-1) receptor agonists are a class of potent anti-glycemic agents to treat diabetes. Recently, several GLP-1 receptor agonists have been found to have cardiovascular benefit independent of their glucose lowing ability. Liraglutide is one of clinically approved effective GLP-1 receptor agonists. In this study, we explored the molecular mechanism of Liraglutide against oxidized low-density lipoprotein (ox-LDL) in cultured endothelial cells. Our data show that Liraglutide treatment ameliorates ox-LDL caused reduction of the transcriptional factor KLF2. In the same experiment, Liraglutide also rescues ox-LDL induced reduction of mitogen-activated protein kinase (MAPK) kinase extracellular signal regulated kinase 5 (ERK5) phosphorylation, and blockage of ERK5 activity by its inhibitor XMD8-92 abolishes the protection of Liraglutide on KLF2 expression. These facts suggest that the action of Liraglutide on endothelial KLF2 is dependent on ERK5. Liraglutide also recovers ox-LDL caused reduction of endothelial tight junctions protein Occludin and ameliorates ox-LDL induced endothelial monolayer permeability increase. On the other hand, Liraglutide inhibits ox-LDL induced expression of vascular adhesion molecules (E-selectin and vascular cell adhesion molecule 1), and prevents ox-LDL induced attachment of monocytes adhesion to endothelial cells. Moreover, Liraglutide mitigates ox-LDL triggered reduction of endothelial nitric oxide synthase (eNOS) expression and NO release. Collectively, our study provides multiple facets of the mechanisms that Liraglutide is a protective agent in endothelial cells and has the potential implication in therapeutic usage of vascular complication in diabetes patients. © 2019 IUBMB Life, 71(9):1347-1354, 2019.

Endothelial Dysfunction

10.1002/iub.2046

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Citations (45)

Confirmation of ‘Pollen- and Seed-Specific Gene Deletor’ System Efficiency for Transgene Excision from Transgenic Nicotiana tabacum under Field Conditions

International Journal of Molecular Sciences (2023)

Zhenzhen Duan Mingyang He Sehrish Akbar Degang Zhao Muqing Zhang

The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-specific Gene Deletor’ system that automatically excised all transgenes from the pollen and seeds of greenhouse-grown transgenic Nicotiana tabacum. In this study, we conducted seven field experiments over three consecutive years to evaluate the stability of transgene excision under field conditions. Our results showed that transgenes were stably excised from transgenic Nicotiana tabacum under field conditions with 100% efficiency. The stability of transgene excision was confirmed based on PCR, as well as the GUS staining patterns of various organs (roots, leaves, petiole, stem, flower, fruit, and seeds) from transgenic N. tabacum. In six transgenic lines (D4, D10, D31, D56, and D43), the transgenes were stably deleted in the T0 and T1 generations. Thus, the ‘Gene Deletor’ system is an efficient and reliable method to reduce pollen- and seed-mediated unintentional gene flow. This system might help to alleviate the food safety concerns associated with transgenic crops.

10.3390/ijms24021160

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Regulation of growth, antioxidant capacity, fatty acid profiles, hematological characteristics and expression of lipid related genes by different dietary n-3 highly unsaturated fatty acids in juvenile black seabream (Acanthopagrus schlegelii)

Aquaculture (2017)

Min Jin You Lu Ye Yuan Yi Li Hong Qiu

FADS2

Sparidae

Malondialdehyde

10.1016/j.aquaculture.2017.01.004

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Citations (95)

Performance of batch, fed-batch, and continuous A–B–E fermentation with pH-control

Bioresource Technology (2010)

Siyu Li Ranjan Srivastava Steven L. Suib Yi Li Richard S. Parnas

Clostridium acetobutylicum

Chemostat

Acidogenesis

Dilution

Continuous production

Batch processing

10.1016/j.biortech.2010.12.078

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Citations (156)

Pollination Competition Effects on Gene‐Flow Estimation: Using Regular vs. Male‐Sterile Bait Plants

Agronomy Journal (2006)

Junming Wang Xiusheng Yang Yi Li Phillip F. Elliott

Pollen‐mediated gene flow from transgenic crops is a concern of the scientific community as well as the general public. Although a common practice, the use of male‐sterile bait plants in field trials to demonstrate rates of gene transfer has been questioned due to the lack of pollination competition. However, little direct evidence has been published. Field experiments of male‐sterile and male‐fertile corn bait plants were conducted in 2001 and 2002, respectively, to evaluate the effects of pollination competition on gene‐flow assessment. Male‐sterile bait plants exhibited a significantly higher rate of outcrossing than male‐fertile plants. The results obtained from this study suggest that actual gene flow from transgenic plants to their wild‐type cultivars or relatives is likely to be lower than estimates reported in previous studies using male‐sterile bait plants, and that male‐fertile that is, normal, bait plants should be used in future studies attempting to estimate gene flow.

Outcrossing

10.2134/agronj2005.0104

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Citations (12)

Elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting success

Plant Biotechnology Journal (2017)

Li Wei Chu Fang Sanalkumar Krishnan Junmei Chen Hao Yu

Plant grafting is an important technique for horticultural and silvicultural production. However, many rootstock plants suffer from undesirable lateral bud outgrowth, low grafting success rates or poor rooting. Here, we used a root-predominant gene promoter (SbUGT) to drive the expression of a tryptophan-2-monooxygenase gene (iaaM) from Agrobacterium tumefaciens to increase auxin levels in tobacco. The transgenic plants, when used as a rootstock, displayed inhibited lateral bud outgrowth, enhanced grafting success rate and improved root initiation. However, root elongation and biomass of SbUGT::iaaM transgenic plants were reduced compared to those of wild-type plants. In contrast, when we used this same promoter to drive CKX (a cytokinin degradation gene) expression, the transgenic tobacco plants displayed enhanced root elongation and biomass. We then made crosses between the SbUGT::CKX and SbUGT::iaaM transgenic plants. We observed that overexpression of the CKX gene neutralized the negative effects of auxin overproduction on root elongation. Also, the simultaneous expression of both the iaaM and CKX genes in rootstock did not disrupt normal growth and developmental patterns in wild-type scions. Our results demonstrate that expression of both the iaaM and CKX genes predominantly in roots of rootstock inhibits lateral bud release from rootstock, improves grafting success rates and enhances root initiation and biomass.

Apical dominance

Lateral root

10.1111/pbi.12738

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Citations (23)

An AGAMOUS intron‐driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar

Plant Biotechnology Journal (2016)

Wei Li Wei Hu Chu Fang Longzheng Chen Weibing Zhuang

Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orientation of the PTAG2 intron sequence). In tobacco, expression of the β-glucuronidase gene (uidA) demonstrates that the fPTAG2I promoter is non-floral-specific, while the rPTAG2I promoter is active in floral buds but with no detectable vegetative activity. Under glasshouse conditions, transgenic tobacco plants expressing the Diphtheria toxin A (DT-A) gene driven by the rPTAG2I promoter produced three floral ablation phenotypes: flowerless, neuter (stamenless and carpel-less) and carpel-less. Further, the vegetative growth of these transgenic lines was similar to that of the wild-type plants. In field trials during 2014 and 2015, the flowerless transgenic tobacco stably maintained its flowerless phenotype, and also produced more shoot and root biomass when compared to wild-type plants. In poplar, the rPTAG2I::GUS gene exhibited no detectable activity in vegetative organs. Under field conditions over two growing seasons (2014 to the end of 2015), vegetative growth of the rPTAG2I::DT-A transgenic poplar plants was similar to that of the wild-type plants. Our results demonstrate that the rPTAG2I artificial promoter has no detectable activities in vegetative tissues and organs, and the rPTAG2I::DT-A gene may be useful for producing flowerless poplar that retains normal vegetative growth.

Vegetative reproduction

Agamous

10.1111/pbi.12581

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Citations (12)

Three-dimensional thermochromic liquid crystal elastomer structures with reversible shape-morphing and color-changing capabilities for soft robotics

Soft Matter (2022)

Yi Li Yasmin Teixeira Gina Parlato Jaclyn Grace Fei Wang

Functional structures with reversible shape-morphing and color-changing capabilities are promising for applications including soft robotics and biomimetic camouflage devices. Despite extensive studies, there are few reports on achieving both reversible shape-switching and color-changing capabilities within one structure. Here, we report a facile and versatile strategy to realize such capabilities via spatially programmed liquid crystal elastomer (LCE) structures incorporated with thermochromic dyes. By coupling the shape-changing behavior of LCEs resulting from the nematic-to-isotropic transition of liquid crystals with the color-changing thermochromic dyes, 3D thermochromic LCE structures change their shapes and colors simultaneously, which are controlled by the nematic-isotropic transition temperature of LCEs and the critical color-changing temperature of dyes, respectively. Demonstrations, including the simulated blooming process of a resembled flower, the camouflage behavior of a "butterfly"/"chameleon" robot in response to environmental changes, and the underwater camouflage of an "octopus" robot, highlight the reliability of this strategy. Furthermore, integrating micro-ferromagnetic particles into the "octopus" thermochromic LCE robot allows it to respond to thermal-magnetic dual stimuli for "adaptive" motion and diverse biomimetic motion modes, including swimming, rolling, rotating, and crawling, accompanied by color-changing behaviors for camouflage. The reversibly reconfigurable and color-changing thermochromic LCE structures are promising for applications including soft camouflage robots and multifunctional biomimetic devices.

Camouflage

Thermochromism

Structural Coloration

Morphing

Soft Robotics

Reconfigurability

Biomimetics

10.1039/d2sm00876a

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Citations (23)

Keeping the genie in the bottle: transgene biocontainment by excision in pollen

Trends in biotechnology (2009)