Hongying Zhang

Nanchang University

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i-10 index

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William G. Rice

Aptose Biosciences (United States)

Stephen B. Howell

University of California, San Diego

Andrea Local

Bristol-Myers Squibb (United States)

Cheng‐Yu Tsai

Stanford University

Changsheng Deng

Guangzhou University of Chinese Medicine

Khalid Benbatoul

Aptose Biosciences (United States)

Si Sun

Institute of Optics and Electronics, Chinese Academy of Sciences

Larry A. Gross

University of California, San Diego

Yongxuan Su

University of California, San Diego

Jianping Song

Guangzhou University of Chinese Medicine

Cooperative Institutions

Chinese Academy of Sciences

127

Henan Agricultural University

103

Sichuan University

102

Chinese Academy of Medical Sciences & Peking Union Medical College

Nanjing Medical University

Sun Yat-sen University

Guangzhou University of Chinese Medicine

University of Chinese Academy of Sciences

Zhejiang University

Chinese Academy of Agricultural Sciences

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Analysis on the Application Value of Molecular Diagnostic Technology of Mycobacterium Tuberculosis

SSRN Electronic Journal (2022)

Yan Kong Yang Chen Yinyin Zhu Jun Zhu Dehua Kong

Objective: To analyze the value of molecular biological detection method [loop-mediated isothermal amplification([loop-mediated isothermal amplification(LAMP) and GeneXpert]) and GeneXpert]to improve the etiological detection of pulmonary tuberculosis in hospital.Methods: The sputum samples from 5019 suspected tuberculosis patients were sent to the Tuberculosis Laboratory from January 2016 to December 2020. The samples were detected by sputum smears and solid culture of mycobacterium from January 2016 to June 2018; and by molecular biological detection technology from July 2018 to November 2020. The etiological diagnosis ability was assessed by detection rate of all suspected tuberculosis patients and etiological positive rate of new diagnosed tuberculosis patients.Results: The etiological positive detection rate of suspected tuberculosis patients increased from 12.28% (135/1099) in 2016 to 26.84% (251/935) in 2020; and the etiological positive rate of new diagnosed tuberculosis patients increased from 40.18% (88/219) in 2016 to 57.62% (87/151) in 2020. Before and after molecular biological detection, the etiological positive detection rate of suspected tuberculosis patients was 13.22% (343/2595) and 20.64% (519/2514), respectively, with statistically significant difference (x2 =50.215, P =0.000); the etiological positive rate of new diagnosed tuberculosis patients was 43.13% (223/517) and 55.98% (220/393), respectively, with statistical significance (x2 =14.749, P =0.000). Conclusion: Molecular biological detection technology can improve the ability of tuberculosis etiological diagnosis in hospitals.

Value (mathematics)

10.2139/ssrn.4068652

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Identification and determination of virulence gene for different isolates of E. faecalis originated from infective piglet

Zhongguo shouyi xuebao (2010)

Yabin Wang Liying Chen Hongying Zhang Lei Liu Cheng JinPing

Among the recently isolated Enterococcal strains from some infected swine flocks in Henan province,five representatives of different origins were selected for systemic identification including pathogenicity assessment and virulent gene detection.The results indicated that the pathogen that caused swine infection was Enterococcus faecalis.The isolates showed similar morphological and cultivating characteristics and tolerance of extreme environments,whilst performed differently in carbohydrate fermentation;sensitive to vancomycin,teicoplanin,rifampicin and ampicillin,whereas totally tolerant to common clinical drugs such as erythromycin,kanamycin and tetracycline.The 16S rRNA analysis indicated that these isolates possess high homology to E.faecalis ATCC29212 (99.6%-99.8%),and the nucleotide homology to E.faecalis NC_004668 and AJ301831 strains that are published in GenBank are 99.7%-99.9% and 99.5%-99.7% respectively.Through analysis of virulence phenotype and six main virulent genes and determination of LD50 to mice,we discovered that the five isolates carried different virulent genes,and the HE1 and HE2 strains which carried all the tested genes were the most pathogenically potential,yet the 4-gene carrying HE41 was least capable.The challenge tests were respectively performed with piglets aged 20d with isolates of HE1 and HE5 and all piglets tested were infected and showed clinical symptoms.

Teicoplanin

Enterococcus faecalis

Kanamycin

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The Status and Role of Xiamen Garden Expo in City Regional Real Estate Economy

四川林业科技 (2016)

Song‐He Chen Zhang Wan-qi Huang Quan-neng Bao Yu-hang Hongying Zhang

In this paper,taking Xiamen Park Expo Garden for example,according to the statistical data of many years,analysis and studies were made of the status and role of Garden Expo in city regional real estate (commercial housing) economy in order to provide reference for the sustainable development of Garden Expo in China.

10.16779/j.cnki.1003-5508.2016.01.024

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Figure S3 from APTO-253 Is a New Addition to the Repertoire of Drugs that Can Exploit DNA BRCA1/2 Deficiency

Cheng‐Yu Tsai Si Sun Hongying Zhang Andrea Local Yongxuan Su

<p>Role of ABCG2 in resistance to Fe(253)3.</p>

10.1158/1535-7163.22504891

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Table S3 from APTO-253 Is a New Addition to the Repertoire of Drugs that Can Exploit DNA BRCA1/2 Deficiency

Cheng‐Yu Tsai Si Sun Hongying Zhang Andrea Local Yongxuan Su

<p>ABC transporter family member genes up-regulated in Raji/253R cells</p>

Raji cell

Table (database)

10.1158/1535-7163.22504879

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[Detection and preliminary study of a family carrying a CCR5Δ32 deletional mutation].

PubMed (2012)

Chi Zhou Hao Sun Jia-Xiang Yin Hongying Zhang Keqin Lin

To investigate the frequencies of chemokine (C-C motif) receptor 5 gene (CCR5)Δ32 deletional mutation of in Han and Dai populations from Yunnan province. Immortalized cell lines were derived from a family carrying the CCR5Δ32 mutation.Blood samples of 346 Han and 355 Dai individuals were collected for genotyping. The coding regions of CCR5 gene were amplified with PCR followed by agarose gel electrophoresis. Suspected mutations were verified with DNA sequencing. Immortalized cell lines were constructed by using Epstain Barr virus and cyclosporine A. The difference between the cell lines and original blood samples was verified with PCR.One ethnic Han individual was confirmed to be heterozygous for a deletional mutation by sequencing, which has led to discovery of a family with CCR5Δ32. Nine immortalized cell lines were established from this family, and no difference between the cell lines and original blood samples was detected by PCR.Together with previous reports, this study has indicated a significant difference in CCR5Δ32 among different ethnic groups in China. Established immortalized cell lines can also provide material for future research.

Agarose gel electrophoresis

Immortalised cell line

10.3760/cma.j.issn.1003-9406.2012.04.024

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Comparison of patients with avian influenza A (H7N9) and influenza A (H1N1) complicated by acute respiratory distress syndrome

Medicine (2018)

Hongyan Li Heng Weng Changqing Lan Hongying Zhang Xinhang Wang

The aim of this study was to compare the clinical features of patients with avian influenza A (H7N9) and influenza A (H1N1) complicated by acute respiratory distress syndrome (ARDS). The clinical data of 18 cases of H7N9 and 26 cases of H1N1 with ARDS were collected and compared in the respiratory intensive care unit (RICU) of Fuzhou Pulmonary Hospital of Fujian from March 2014 to December 2016. Patients with H7N9 had a higher acute physiology and chronic health evaluation-II score (P < .05) and lung injury score (P < .05). The rates of coexisting diabetes mellitus, hyperpyrexia, and bloody sputum production were significantly higher in the H7N9 group than in the H1N1 group (P < .05). The H7N9 group had a longer duration of viral shedding from the onset of illness (P < .05) and from the initiation of antiviral therapy (P < .05) to a negative viral test result than the H1N1 group. Patients with H7N9 had higher rates of invasive mechanical ventilation; serious complications, including alimentary tract hemorrhage, pneumothorax or septum emphysema, hospital-acquired pneumonia (HAP) and multiple organ dysfunction syndrome (MODS); and hospital mortality (P < .05). At the 6th month of follow-up, the rates of bronchiectasia, reticular opacities, fibrous stripes, and patchy opacities on chest computed tomography (CT) were significantly higher in the H7N9 group than in the H1N1 group (P < .05). Based on multiple logistic regression analysis, H7N9 influenza viral infection was associated with a higher risk of the presence of severe ARDS than H1N1 influenza viral infection (odds ratio 8.29, 95% confidence interval [CI] 1.53–44.94; P < .05). Compared to patients with H1N1, patients with H7N9 complicated by ARDS had much more severe disease. During long-term follow-up, more changes in pulmonary fibrosis were observed in patients with H7N9 than in patients with H1N1 during the convalescent stage.

2019-20 coronavirus outbreak

H1N1 influenza

10.1097/md.0000000000010194

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Citations (37)

Integrating Semantic Information into Multiple Kernels for Protein-Protein Interaction Extraction from Biomedical Literatures

PLoS ONE (2014)

Lishuang Li Panpan Zhang Tianfu Zheng Hongying Zhang Zhenchao Jiang

Protein-Protein Interaction (PPI) extraction is an important task in the biomedical information extraction. Presently, many machine learning methods for PPI extraction have achieved promising results. However, the performance is still not satisfactory. One reason is that the semantic resources were basically ignored. In this paper, we propose a multiple-kernel learning-based approach to extract PPIs, combining the feature-based kernel, tree kernel and semantic kernel. Particularly, we extend the shortest path-enclosed tree kernel (SPT) by a dynamic extended strategy to retrieve the richer syntactic information. Our semantic kernel calculates the protein-protein pair similarity and the context similarity based on two semantic resources: WordNet and Medical Subject Heading (MeSH). We evaluate our method with Support Vector Machine (SVM) and achieve an F-score of 69.40% and an AUC of 92.00%, which show that our method outperforms most of the state-of-the-art systems by integrating semantic information.

Tree kernel

Kernel (algebra)

Graph kernel

Tree (set theory)

Similarity (geometry)

10.1371/journal.pone.0091898

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Analysis of the Application Value of Molecular Diagnostic Technology of Mycobacterium Tuberculosis

SSRN Electronic Journal (2022)

Yinyin Zhu Jianming Wang Mengkai Qiao Minghe Guo Yan Kong

Background: To evaluate the application value of molecular diagnostic technology for tuberculosis.Methods: Firstly, the sensitivity and specificity were compared between sputum smear and isothermal amplification (IA, Di’ao Biological Technology Co., Ltd, Guangzhou, China) with solid culture as the standard method. Secondly, the detection rate was compared between liquid culture and molecular diagnostic methods, including IA and Xpert MTB/RIF. Thirdly, the minimum detection limit of IA and Xpert MTB/RIF was compared. Finally, IA was used from 2019 to February 2020, and Xpert MTB/RIF was used from March 2020, the contribution of molecular diagnosis to the etiological diagnosis of tuberculosis in Lishui District in Nanjing was compared in 2019 and 2020.Results: The sensitivity of IA and sputum smear was 100% and 54.55%, respectively (P < 0.05), and the specificity was 97.66% and 98.44%, respectively (P >0.05). IA was more consistent with solid culture (Kappa =0.924) than smear (Kappa =0.624). The detection rates of IA, Xpert MTB/RIF and liquid culture were 75.00%, 77.50% and 77.50%, respectively (P > 0.05). The minimum detection limit of Xpert MTB/RIF was lower than that of IA. In 2019 and 2020, molecular diagnosis accounted for 31.58% and 39.28% of positive etiology, respectively, and the incidence of positive molecular pathogens was 1.25/100,000 and 2.24/100,000, respectively. Conclusion: Molecular detection technology improves the detection rate of tuberculosis etiology. Each TB designated detection hospital can select molecular detection methods according to local conditions, which will help control the spread of tuberculosis.

Value (mathematics)

10.2139/ssrn.4097533

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[Heat-induced antigen retrieval in immunohistochemistry for estrogen and progesterone receptors in breast cancer tissues].

PubMed (2011)

Xinjun Li Bing Wei Huijiao Chen Zhang Zhang Hongying Zhang

免疫组织化学是目前乳腺癌激素受体检测的常规方法,但免疫组织化学检测易受多种因素的影响,其标准化程度令人担忧.英国国家免疫细胞化学外部质量评估方案(NEQAS-ICC)曾评估200余个实验室对同一例乳腺癌组织进行的雌激素受体(ER)染色,发现仅36%的实验室获得准确的检测结果[1],提示抗原修复是影响免疫组织化学检测结果最主要的因素,而激素受体水平低的癌组织进行免疫组织化学染色更易受到各种因素的影响。

Antigen retrieval

Progesterone receptor

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Citations (3)