nucleopolyhedrovirus (BmNPV) is a pathogen that causes great economic losses in sericulture. Many genes play a role in viral infection of silkworms, but silkworm metabolism in response to BmNPV infection is unknown. We studied BmE cells infected with BmNPV. We performed liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)-based non-targeted metabolomics analysis of the cytosolic extract and identified 36, 76, 138, 101, 189, and 166 different molecules at 3, 6, 12, 24, 48, and 72 h post BmNPV infection (hpi) compared with 0 hpi. Compounds representing different areas of metabolism were increased in cells post BmNPV infection. These areas included purine metabolism, aminoacyl-tRNA biosynthesis, and ABC transporters. Glycerophosphocholine (GPC), 2-hydroxyadenine (2-OH-Ade), gamma-glutamylcysteine (γ-Glu-Cys), hydroxytolbutamide, and 5-pyridoxolactone glycerophosphocholine were continuously upregulated in BmE cells post BmNPV infection by heat map analysis. Only 5-pyridoxolactone was found to strongly inhibit the proliferation of BmNPV when it was used to treat BmE cells. Fewer infected cells were detected and the level of BmNPV DNA decreased with increasing 5-pyridoxolactone in a dose-dependent manner. The expression of BmNPV genes ie1, helicase, GP64, and VP39 in BmE cells treated with 5-pyridoxolactone were strongly inhibited in the BmNPV infection stage. This suggested that 5-pyridoxolactone may suppress the entry of BmNPV. The data in this study characterize the metabolism changes in BmNPV-infected cells. Further analysis of 5-pyridoxolactone, which is a robust antiviral molecule, may increase our understanding of antiviral immunity.
The skin is highly susceptible to friction during labor or outdoor activities, leading to blisters, bleeding, pain, and infection. In this paper, a butyl-cyanoacrylate/polymethylmethacrylate (504/PMMA) composite was developed as a protectant to prevent skin damage caused by external friction. The composite could rapidly solidify on the skin surface, forming a strong polymer film. Results from a rat model showed that the 504/PMMA composite effectively prevented skin damage and that increasing the proportion of PMMA improved its anti-friction performance. Moreover, the material is biocompatible. In this study, we offered new options for reducing skin friction and blisters, which have potential to practical applications.
Aluminium titanate (AT) ultrafine powder was prepared via hydrolytic sol-gel (HSG) method using aluminium nitrate (Al (NO 3 ) 3 ·9H 2 O) and titanium tetrachloride (TiCl 4 ) as precursors as well as ethanol as solvent. Water required for hydrolysis reaction was supplied by the crystal water of aluminium nitrate itself. The effect of gelation processes, i.e. reflux and solvothermal treatment, on synthesis of AT powder was investigated by means of DTA-TG, XRD, SEM, and TEM, etc. The result shows that the gelation process has significant effect on the synthesis of AT powder. By reflux process, AT powder was synthesized at 1350 °C with average particle size above 1μm and serious agglomeration. Through solvothermal treatment process, however, AT powder with average particle size less than 150 nm was prepared at 1050 °C at a relatively high synthesis rate, which is due to the refinement reactants by the solvothermal treatment.
Metal–organic frameworks (MOFs) supported metal nanoparticles (MNPs) (MNPs@MOFs) composites, as a class of effective functional materials, have been extensively explored because of the synergetic effect of MOFs and MNPs. Lots of synthesized methods are developed to synthesize MNPs@MOFs composites. These strategies can be generally divided into four main synthesis strategies: ship‐in‐the‐bottle, bottle‐around‐the‐ship, one‐pot, and sandwich assembly approaches. In this review, the recent synthesized strategies are discussed and summarized for the design and fabrication of different MNPs@MOFs composites. Then, the catalytic applications of some MNPs@MOFs composites are presented. Lastly, the challenges and future perspectives are also pointed out in this hot research filed.
In this study, we investigate the feasibility of the use of aminolevulinic acid hydrochloride (5-ALA)-induced fluorescence in the detection of parathyroid glands in rats.The intraoperative identification of parathyroid glands is difficult, which is one of the main reasons for postoperative hypoparathyroidism.An animal experiment was designed. Ten Sprague-Dawley (S-D) adult rats were injected intraperitoneally with a 300 mg/kg dosage of 5-ALA. Anesthesia and surgical dissection were performed to expose the thyroid gland 1 h later. Blue light with a wavelength of 405±3 nm was used to light the trachea bilaterally in order to detect parathyroid fluorescence by direct vision. Histology of the fluorescent tissue was performed to confirm parathyroid identification.Parathyroid gland fluorescence was visualized in all 10 S-D rats. The red fluorescence of the parathyroid glands was located on the posterolateral thyroid glands and could be seen with the naked eye. Histology of the fluorescent tissue confirmed the identification of parathyroid glands under light microscopy.5-ALA induced fluorescence of parathyroid glands can clearly differentiate them from the surrounding tissue by direct vision alone. This method could be used intraoperatively and has potential clinical value.
Y. L. Zhao and colleagues develop biocompatible, uniform, and redispersible mesoporous silica nanoparticle-based nanocarriers towards controlled and cancer-targeted drug delivery for significantly inhibiting the tumor growth in vivo. On page 2450, the research team demonstrates a successful cancer therapy protocol of 1) intravenous injection of drug-loaded nanocarriers, 2) controlled and targeted drug delivery to tumor sites for therapy, and 3) excretion of the nanocarriers from the animal bodies after the drug delivery.
OBJECTIVE:To study the expression and significance of Ezrin in adenoid cystic carcinoma of salivary gland. METHODS: The expression of Ezrin was detected by the immunohistochemical method in adenoid cystic carcinoma of salivary gland and normal salivary gland tissues, and the correlations among the expression of Ezrin in adenoid cystic carcinoma of salivary gland and invasion, occurrence, metastasis and prognosis were analyzed. RESULTS: The over expression rates of Ezrin in adenoid cystic carcinoma of salivary gland and normal salivary gland tissues were 44.68% and 9.09%, respectively, P0.05; in the adenoid cystic carcinoma tissue, strong Ezrin immunostaining was detected mainly in the cytoplasma, whereas in the normal salivary gland tissue, Ezrin immunostaining was observed principally in the membrane and varied in intensity from weak to negative. The intensity of Ezrin expression was associated with the presence or absence of nerve invasion, locoregional recurrence and distant metastasis, P0.05. The 5-year cumulative survival rates in positive Ezrin expression group and negative Ezrin expression group were 66.70% and 100.00%, respectively; 10-year cumulative survival rates were 27.83% and 95.00%, respectively, and the positive Ezrin expression group had worse prognosis than the negative one, P0.05. CONCLUSION: The intensity of Ezrin expression is correlated with the genesis and ability of invasion, occurrence and metastasis of adenoid cystic carcinoma of salivary gland, and its positive expression indicates poor prognosis.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)