Lu Zhang

First Affiliated Hospital of GuangXi Medical University

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Yuguang Zhang

Yanshan University

Liying Cheng

Shanghai Ninth People's Hospital

Xueqian Xie

Shanghai Jiao Tong University

Lingyun Wang

Ruijin Hospital

Xiaoming Sun

Shanghai Jiao Tong University

Guangdong Zhou

Shanghai Jiao Tong University

Keke Zhao

Henan Cancer Hospital

Yilin Cao

Shanghai Jiao Tong University

Wenguo Cui

Ruijin Hospital

Wenjie Zhang

Sichuan University

Cooperative Institutions

Shanghai Jiao Tong University

240

Chinese Academy of Sciences

Ruijin Hospital

Sichuan University

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

Shanghai Ninth People's Hospital

Sun Yat-sen University

Renji Hospital

University of Chinese Academy of Sciences

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Two-dimensional electrospun nanofibrous membranes for promoting random skin flap survival

RSC Advances (2016)

Xiaoming Sun Reila Zheng Liying Cheng Xin Zhao Rong Jin

Electrospun fibrous membranes made of natural materials are more advantageous for random skin flap survival, and can be used as carrier implantation materials for improving skin flap survival rate.

Electrospinning

Skin flap

Artificial skin

10.1039/c5ra23034a

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Citations (18)

Self-assembled anionic and cationic Au nanoparticles with Au nanoclusters for the exploration of different biological responsiveness in cancer therapy

Nanoscale Advances (2021)

Jingjing Yang Lu Zhang Qian Zhou Fan Chen Martina H. Stenzel

Self-assembly overcomes the biodegradation resistance of some traditional inorganic drug carriers. Herein, we prepared self-assembled Au nanocluster-based nanoparticles with different sizes and charges based on solvent- and cation-induced self-assembly nanotechnology as anti-cancer drug vehicles to solve the potential metabolism problems of solid gold nanoparticles. We also systematically explored the responsiveness of cancer cells to self-assembled Au nanocluster-based nanoparticles with different sizes and surface modified properties. We discovered that self-assembled nanoparticles inherited molecular-like properties of small-size Au NCs and exhibited an aggregation-induced emission (AIE) phenomenon with intense luminescence. Self-assembled Au nanocluster-based nanoparticles (Au NPs and cAu NPs) taking advantage of their size and positive charge exhibited better cell uptake than Au NCs. Encouraged by the excellent biological compatibility and cell uptake of these nanomaterials, we prepared drug-loaded nanomaterials by diffusion absorption and hydrophobic-induced embedding. cAu NPs@DOX showed an excellent anti-cancer effect owing to efficient cell internalization; Au NPs@DOX exhibited slow release of cargo drugs which might be significant to in vivo drug delivery. This work plays a crucial role in the rational design of self-assembled multifunctional gold-based nanoparticles in the application of nanomaterial-assisted multifunctional drug delivery systems (DDSs).

Nanomaterials

Nanoclusters

Internalization

10.1039/d0na01066a

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Citations (12)

A Handheld Contactless Conductivity Detector for Monitoring the Desalting of Low-Volume Virus and Cell Samples

Yiren Cao Zhimin Tao Youli Tian Ke‐Er Chen Lu Zhang

Desalting of biosamples is essential for analytical techniques intolerant to abundant salts. However, there is no simple tool to monitor the desalting of low-volume samples so far. Here we developed a handheld capacitively coupled contactless conductivity detector (hC4D) to measure the conductivity of 4-102 Î¼L of biosamples. One can easily hold the hC4D and pipette the sample into the detection window. After conductivity measurement, the results were transmitted to the smartphone for calibration and recording. To customize the desalting procedures for blood, virus, and cell samples, we used the hC4D to monitor the progress of gel filtration (GF), ultrafiltration (UF), and dialysis. Three rounds of GF and UF completely removed the salts but led to poor sample recovery. In contrast, low concentrations of residual salts remained and better recovery rates were achieved after two rounds of GF and UF. We further utilized the hC4D to monitor the dialysis in real time and tuned the salt concentration in the cell sample. The hC4D is a promising tool to balance the need for low salt concentration and good sample quality.

10.2139/ssrn.4394406

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A Novel Way for Upper Eyelid Rejuvenation by Combination of Local Fat-Fascia-Muscle Flap Repositioning for Middle-Aged Asian Women

Journal of Plastic Reconstructive & Aesthetic Surgery (2019)

Xiyuan Mao Yunkun Pei Lu Zhang Ying Zhang Rong Jin

Rejuvenation

Orbicularis oculi muscle

Upper trunk

Blepharoplasty

10.1016/j.bjps.2019.09.004

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Citations (5)

Comparison of three types of chondrocytes in collagen scaffolds for cartilage tissue engineering

Biomedical Materials (2009)

Lu Zhang Myron Spector

The objective of this study was to compare the chondrogenesis in type I and II collagen scaffolds seeded with chondrocytes from three types of cartilage, after four weeks of culture: auricular (AU), articular (AR) and meniscal (ME). Related aims were to investigate the expression of a contractile muscle actin isoform, α-smooth muscle actin (SMA), in the cells in the scaffold and to determine the presence of a lubricating glycoprotein, lubricin, in the constructs. Adult goat AU, AR and ME chondrocytes were seeded into two types of collagen scaffolds: type II collagen and type I/III collagen. After four weeks of culture, the constructs were prepared for histochemical and immunohistochemical analysis of the distribution of glycosaminoglycan (GAG), types I and II collagen, elastin, SM and lubricin. AU constructs contained substantially more tissue than the AR and ME samples. The AU constructs exhibited neocartilage, but no elastin. There were no notable differences between the type I and II collagen scaffolds. Novel findings were the expression of SMA by the AU cells in the scaffolds and the presence of lubricin in the AR and AU constructs. AU cells have the capability to produce cartilage in collagen scaffolds under conditions in which there is little histogenesis by AR and ME cells.

Chondrogenesis

Type II collagen

Type I collagen

Histogenesis

10.1088/1748-6041/4/4/045012

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Citations (40)

Multifunctional integrally-medicalized hydrogel system with internal synergy for efficient tissue regeneration

Chemical Engineering Journal (2020)

Liucheng Zhang Lu Chen Yi Xiang Zhimo Liu Xiyuan Mao

Biocompatibility

Gelatin

Bovine serum albumin

10.1016/j.cej.2020.126839

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Citations (35)

Prevalence, awareness, treatment and control of hypertension in elderly Chinese

Hypertension Research (2013)

Chang‐Sheng Sheng Ming Liu Yuan‐Yuan Kang Fang‐Fei Wei Lu Zhang

Sitting

Antihypertensive drug

Thiazide

Calcium channel blocker

Stepwise regression

10.1038/hr.2013.57

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Citations (60)

[Clinical study of polymerase chain reaction technique in the diagnosis of bone tuberculosis].

PubMed (1997)

Sun Yu Y Zhang Lu Zhang

To evaluate the clinical value of polymerase chain reaction (PCR) technique in diagnosis of bone tuberculosis.PCR, standard light-microscopy and standard culture technique were used to detect Mycobacterium tuberculosis in samples obtained from 60 patients with bone tuberculosis and 20 patients without bone tuberculosis. In the meantime, some factors affecting PCR result were analysed and methods to deal with them were discussed.In the group of 60 patients with bone tuberculosis, the positive rate was 83% in PCR technique, 3% in standard light-microscope technique and 7% in the standard culture technique. A statistically obvious difference was seen (P < 0.005). In the group of 20 patients without bone tuberculosis, 2 cases showed positive in PCR technique, none in the other methods. Specificity of PCR technique in a blind comparison study indicated 100%. The whole process of PCR amplification is fully automatic and can be finished within several hours, and the detection time is considerably reduced.PCR technique is a rapid, specific, sensitive and simple method for detection of mycobacterium tuberculosis in sample of bone tuberculosis, and it is of great value in the diagnosis of bone tuberculosis and differentiating bone tuberculosis from other bone diseases.

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USP47 deficiency in mice modulates tumor infiltrating immune cells and enhances antitumor immune responses in prostate cancer

Cancer Immunology Immunotherapy (2024)

Wang Qian-lan Shunyuan Lu Dandan Xu Jinxia Ma Rui Guo

Abstract This study investigates the role of USP47, a deubiquitinating enzyme, in the tumor microenvironment and its impact on antitumor immune responses. Analysis of TCGA database revealed distinct expression patterns of USP47 in various tumor tissues and normal tissues. Prostate adenocarcinoma showed significant downregulation of USP47 compared to normal tissue. Correlation analysis demonstrated a positive association between USP47 expression levels and infiltrating CD8 + T cells, neutrophils, and macrophages, while showing a negative correlation with NKT cells. Furthermore, using Usp47 knockout mice, we observed a slower tumor growth rate and reduced tumor burden. The absence of USP47 led to increased infiltration of immune cells, including neutrophils, macrophages, NK cells, NKT cells, and T cells. Additionally, USP47 deficiency resulted in enhanced activation of cytotoxic T lymphocytes (CTLs) and altered T cell subsets within the tumor microenvironment. These findings suggest that USP47 plays a critical role in modulating the tumor microenvironment and promoting antitumor immune responses, highlighting its potential as a therapeutic target in prostate cancer.

10.1007/s00262-024-03730-5

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R11 Peptides Can Promote the Molecular Imaging of Spherical Nucleic Acids for the Bladder Cancer Margin Identification

SSRN Electronic Journal (2021)

Minghai Ma Pu Zhang Xiao Liang Daxiang Cui Qiuya Shao

One of the critical problems in bladder cancer (BC) management is local recurrence of disease. However, achieving accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition technology. Herein, survivin molecular beacon (MB) and R11 peptides-linked spherical mucleic acids (SNAs) were synthesized as a nanoprobe (AuNP-MB@R11) for sensitive detection of BC margins. Physicochemical Properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of SNAs. The AuNP-MB@R11 had good stability against nuclease, high sensitivity and specificity to detect survivin ssDNA in vitro experiment. In cytology, R11 peptides could increase BC targeting ability and membrane penetrability of SNAs. Notably, R11 peptides can significantly promote the disintegration of lysosome and release of SNAs to enhance the fluorescence imaging quality. The further RNA sequencing proved that some genes related with endocytosis and lysosome regulated significantly, such as AGPAT5, GPD1L, TREX1 and APEX1. In orthotopic BC models and a clinical sample from patient with BC, AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection effects compared to those achieved by SNAs. Thus, R11 peptides-linked SNAs presented a promising potential to identify the BC margin, which may help to improve the R0 resection rate in surgery and improve patient’s quality of life.

Identification

Margin (machine learning)

10.2139/ssrn.3825979

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Citations (2)