Jing Wang

Shandong First Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Qiyu Bo

Shanghai First People's Hospital

Xiaodong Sun

Shanghai First People's Hospital

Junran Sun

Shanghai Jiao Tong University

Nana Xu

Zunyi Medical University

Minwen Zhou

Shanghai Jiao Tong University

Xi Shen

Ruijin Hospital

Tao Sun

China Pharmaceutical University

Yisheng Zhong

Sichuan University

Xiaowei Tong

Shanghai Children's Hospital

Hong Zhu

The People's Hospital Tongling

Cooperative Institutions

Shanghai Jiao Tong University

167

Sun Yat-sen University

Shanghai First People's Hospital

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Fudan University

Zhejiang University

Peking Union Medical College Hospital

Sichuan University

Central South University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Autophagy activated by SIRT6 regulates Aβ induced inflammatory response in RPEs

Biochemical and Biophysical Research Communications (2018)

Yiji Feng Jian Liang Yuanqi Zhai Junran Sun Jing Wang

Proinflammatory cytokine

Sirtuin 1

SIRT2

10.1016/j.bbrc.2018.01.159

Cite

Citations (16)

The association between decreased choriocapillary flow and electroretinogram impairments in patients with diabetes

Photodiagnosis and Photodynamic Therapy (2023)

Xiaowen Deng Zijing Li Peng Zeng Jiaqi Liang Jing Wang

Electroretinography

Erg

10.1016/j.pdpdt.2023.103547

Cite

Citations (7)

Association between retinol binding protein 4 and diabetic retinopathy among type 2 diabetic patients: a meta-analysis

Acta Diabetologica (2020)

Wentao Han Huagen Wei Weizheng Kong Jing Wang Luqian Yang

Funnel plot

Subgroup analysis

10.1007/s00592-020-01535-3

Cite

Citations (7)

MicroRNA-376b-3p Suppresses Choroidal Neovascularization by Regulating Glutaminolysis in Endothelial Cells

Investigative Ophthalmology & Visual Science (2023)

Yifan Feng Liyang Wang Chunqiong Dong Xi Yang Jing Wang

Purpose: Choroidal neovascularization (CNV) is a common pathological change of various ocular diseases that causes serious damage to central vision. Accumulated evidence shows that microRNAs (miRNAs) are closely related with the regulation of endothelial metabolism, which plays crucial roles in angiogenesis. Here, we investigate the molecular mechanism underlying the regulation of endothelial glutamine metabolism by miR-376b-3p in the progression of CNV. Methods: Human retinal microvascular endothelial cells (HRMECs) were transfected with control or miR-376b-3p mimics, and the expression of glutaminase 1 (GLS1), a rate-limiting enzyme in glutaminolysis, was detected by real-time PCR or Western blotting. The biological function and glutamine metabolism of transfected HRMECs were measured by related kits. Luciferase reporter assays were used to validate the CCAAT/enhancer-binding protein beta (CEBPB) was a target of miR-376b-3p. Chromatin immunoprecipitation and RNA immunoprecipitation assays were performed to verify the binding of CEBPB on the promoter region of GLS1. Fundus fluorescein angiography and immunofluorescence detected the effect of miR-376b-3p agomir on rat laser-induced CNV. Results: The expression of miR-376b-3p was decreased, whereas GLS1 expression was increased in the retinal pigment epithelial–choroidal complexes of rats with CNV. HRMECs transfected with miR-376b-3p mimic showed inhibition of CEBPB, resulting in the inactivation of GLS1 transcription and glutaminolysis. Moreover, the miR-376b-3p mimic inhibited proliferation, migration and tube formation but promoted apoptosis in HRMECs, whereas these effects counteracted by α-ketoglutarate supplementation or transfection with CEBPB overexpression plasmid. Finally, the intravitreal administration of the miR-376b-3p agomir restrained CNV formation. Conclusions: Collectively, miR-376b-3p is a suppressor of glutamine metabolism in endothelial cells that could be expected to become a therapeutic target for the treatment of CNV-related diseases.

Chromatin immunoprecipitation

10.1167/iovs.64.1.22

Cite

Citations (3)

The dynamic changes of macrophage morphology in progression of choroidal neovascularization in laser-induced choroidal neovascularization mice model

Research Square (Research Square) (2023)

Nana Xu Tao Sun Yulan Wang Xiaowei Tong Shiheng Lu

Abstract Background: Neovascular AMD is responsible for the majority of severe vision loss which is mainly caused by choroidal neovascularization (CNV) . It persists or recurs in a subset of patients and regression at 5 years of anti-VEGF treatment or later. The precise mechanisms contribute to CNV continue to be elucidated. According to our previous studies, macrophage play a critical role in CNV, herein, we aim to unveil the conceret morphological changes of macrophage in CNV process to help us understand the dynamic changes intuitively. Methods: Mice were subjected to laser injury to induce CNV, and lesion expansion and macrophages , transformation were tracked using immunofluorescence and confocal analysis. Several strategies were taken to verify the dynamic changes of macrophage: Immunofluorescence and confocal assays were performed on choroidal flat-mount to evaluate the morphologe and phenotype of macrophage in different CNV phase and further certified with western-blot and RT-PCR. Results: Location of infiltrated macrophages was dynamic after laser injury in CNV mice model and morphology of macrophages was also in dynamic changing. Branching macrophage were gradually shift to be round with the progression of CNV which were certificated to be M2 phenotype shift. Conclusions: The dynamic changes of macrophage morphology were obviously in CNV formation and round-shaped M2 phenotype was proved to promote neovascularization. In general, the changes of morphology we found in this study can further help us to know the critical role macrophages play in CNV progression and to exploite the potential treatment option for CNV implied by macrophage polarity shift.

Immunofluorescence

10.21203/rs.3.rs-2892025/v1

Cite

Citations (0)

Dynamic versus Static Ultra-widefield Fluorescein Angiography in Eyes with Diabetic Retinopathy: A Pilot Prospective Cross-sectional Study

Research Square (Research Square) (2020)

Hangqi Shen Jing Wang Tian Niu Jili Chen Xun Xu

Abstract Background: Dynamic angiography can present additional information. However, evidence-based support in clinical application of dynamic modalities remain insufficient. The purpose of this study is to analyze differences in ultra-widefield fluorescein angiography (UWFA) findings between time-lapse dynamic and static imaging in eyes with diabetic retinopathy (DR). Methods: Twenty-eight eyes of 28 DR patients undergoing UWFA were included in the cross-sectional study. A series of UWFA images from each patient were converted into a time-lapse video in a Moving Picture Experts Group 4 movie format as a dynamic image. A single clear arteriovenous phase image was chosen as a static UWFA image. Non-perfusion index (NPI) and its correlation with intraretinal microvascular abnormality and neovascularization in different retinal zones were compared between dynamic and static UWFA imaging in severe nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR). Results: NPI appeared to increase from the center to the far-periphery in both groups. Dynamic NPI appeared to be lower in the total retinal area (0.25 vs 0.28, p =0.005), far-periphery (0.30 vs 0.34, adjusted p =0.040) and superior quadrant (0.27 vs 0.34, adjusted p =0.045) in contrast to static NPI. Far-peripheral NPI was associated with intraretinal microvascular abnormality in posterior area in both groups. Conclusion: Time-lapse dynamic UWFA imaging is a useful method in the differential diagnosis of hypofluorescence in the most peripheral region, which may provide a reliable measurement of NPI and improve the diagnostic accuracy of DR.

Abnormality

10.21203/rs.3.rs-51867/v1

Cite

Citations (0)

Sevoflurane attenuate hypoxia-induced VEGF level in tongue squamous cell carcinoma cell by upregulating the DNA methylation states of the promoter region

Biomedicine & Pharmacotherapy (2015)

Yi Lu Jing Wang Jia Yan Yaqiong Yang Yu Sun

Hypoxia

10.1016/j.biopha.2015.02.032

Cite

Citations (21)

Associations of DNA methylation algorithms of aging with cardiovascular disease and mortality risk among US older adults

Research Square (Research Square) (2024)

Xiangwei Li Hui Zhang Yulu Gong D.H Hao Yaqian Xu

Abstract Recently, several DNA methylation (DNAm) algorithms have emerged as strong predictors of adverse health outcomes in older adults, offering insights into cardiovascular disease (CVD) risk stratification. Using data from the National Health and Nutrition Examination Survey (1999–2002), we assessed twelve DNAm algorithms (HannumAgeacc, PhenoAgeacc, GrimAgeMortacc, GrimAge2Mortacc, among others) in relation to CVD risk and mortality. The analysis included two subsets (n = 1,230 for CVD risk and n = 1,606 for CVD mortality). Significant associations were found between GrimAgeMortacc and GrimAge2Mortacc with coronary heart disease and heart attack risk. Additionally, HannumAgeacc, PhenoAgeacc, GrimAgeMortacc, and GrimAge2Mortacc showed significant associations with CVD mortality, with multivariable-adjusted hazard ratios per standard deviation increase of 1.15 (95% CI = 1.02–1.28), 1.15 (95% CI = 1.03–1.28), 1.72 (95% CI = 1.38–2.14), and 1.89 (95% CI = 1.52–2.29), respectively. DNAm algorithms, particularly GrimAgeMortacc and GrimAge2Mortacc, may serve as valuable tools for CVD risk stratification and mortality assessment.

dNaM

Risk Stratification

10.21203/rs.3.rs-5191920/v1

Cite

Citations (0)

A specific RIP3 ⁺ subpopulation of microglia promotes retinopathy through a hypoxia-triggered necroptotic mechanism

Proceedings of the National Academy of Sciences (2021)

Chang He Yan Liu Zijing Huang Ziqi Yang Tian Zhou

Significance Retinopathy is the leading cause of blindness, and development of effective therapy is urgently needed. Here, we defined an unprecedented subgroup of microglia that is responsible for causing retinopathy under hypoxia. Mechanistic studies demonstrated the signaling pathway of hypoxia-induced necroptosis of retinal microglia, i.e., the hypoxia–RIP1–RIP3–MLKL signaling axis, triggered an explosive release of FGF2, which in its turn to induce retinal neovascularization. Simultaneous targeting of necroptosis–FGF2 pathway and VEGF produces synergistic effects for treating retinopathy. On the basis of our findings, we propose a concept of necroptotic microglia-induced retinal angiogenesis and highlight a combination therapy for effective treatment of retinopathy.

Hypoxia

10.1073/pnas.2023290118

Cite

Citations (60)

Intravitreal conbercept for branch retinal vein occlusion induced macular edema: one initial injection versus three monthly injections

BMC Ophthalmology (2020)

X. Chen Teh‐Min Hu Jian Zuo Hong-Fei Wu Zhihong Liu

Abstract Background To compare the efficacy of one initial intravitreal injection of conbercept (IVC) versus three monthly IVCs in patients with macular edema (ME) after branch retinal vein occlusion (BRVO). Both options were followed by a pro re nata (PRN) retreatment regimen. Methods This study retrospectively investigated and followed 60 patients with acute ME secondary to BRVO for over a year. 30 subjects received one initial injection (1 + PRN group); while, 30 received three monthly injections (3 + PRN group). The functional and anatomic outcomes were assessed during each follow-up. Results The general characteristics of the 60 subjects were as follows: mean [SD] age, 57.43 [13.06] years; 33 [55%] female; 36 [60%] non-ischemic form. Both groups showed a stable gain in visual acuity (VA) with similar logMAR (mean ± SD) (1 + PRN group 0.308 ± 0.399, 3 + PRN group 0.34 ± 0.352) during the first 12 months. Additionally, both groups exhibited a significant reduction in central foveal thickness (CFT) with no statistically significant difference between them (1 + PRN group 222.1 μm ± 197.1 μm , 3 + PRN group 228.4 μm ± 200.2 μm ). Both treatment groups had similar improvements in logMAR and anatomic outcomes over time. The stratified analysis showed that patients with the non-ischemic form and those with the ischemic form had similar improvements in VA (0.346 ± 0.366 VS 0.29 ± 0.39, P = 0.575) during the 12 months follow-ups. The number of injections was lower in the 1 + PRN group (4.0 ± 1.6) than in the 3 + PRN group (4.7 ± 1.3) ( P = 0.068). No adverse effects or unexpected safety issues were reported in either group. Conclusions Conbercept yielded significant improvements in VA and CFT among patients with BRVO induced ME, independent of their retinal ischemia status. The results showed that the 3 + PRN regimen do not lead to better functional outcomes or lower treatment needs in clinical practice as compared to the 1 + PRN regimen.

Pro re nata

10.1186/s12886-020-01494-x

Cite

Citations (6)