Xiaolong Li

Peking University

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Emmanuel Martínez-Ledesma

Tecnológico de Monterrey

Siyuan Zheng

Fujian University of Technology

Dimpy Koul

The University of Texas MD Anderson Cancer Center

Nghi Nguyen

The University of Texas Southwestern Medical Center

Feng Gao

Tianjin University

Stephan C. Clifford

Texas A&M University

Shaofang Wu

Fujian Medical University

Jie Ding

German Cancer Research Center

Hong Li

Inner Mongolia University

Keith L. Ligon

Brigham and Women's Hospital

Cooperative Institutions

Ministry of Agriculture and Rural Affairs

Gansu Agricultural University

Zhejiang A & F University

Lanzhou University

Zhejiang University

Chinese Academy of Sciences

Peking University

Hebei Medical University

Institute of Grassland Research

State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems

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Determination of ursolic acid and oleanolic acid contents in Kuding tea of Aquifoliaceae.

Medicinal plant (2010)

Hongyang Li Fei Liu Fengqin Zhang Xiaolong Li Guomin Liu

[Objective] The research aimed at determining the contents of ursolic acid and oleanolic acid in different species and different origins of Kuding tea and providing basic data for the industrialization development of ursolic acid.[Method] The contents of ursolic acid and oleanolic acid in functional leaves from 6 species of Kuding tea in Aquifoliaceae and 4 kinds of the commercial Ilex kudingcha C.J.Tseng shoots were determined by high performance liquid chromatography(HPLC).The chromatographic conditions were as follows:Eclipse XDB-C18 column(5 μm,4.6 mm×150 mm),mobile phases of methanol-0.2% aqueous solution of phosphoric acid(v:v=88:12),detection wavelength of 220 nm,flow rate of 0.8 ml/min,column temperature of 35 ℃ and injection volume of 20 μl.[Result] The contents of ursolic acid from 6 species of Kuding tea in Aquifoliaceae were as follows:I.cornuta Lindl.(1.64%)I.huoshanesis Y.H.He(1.63%)I.latifolia Thunb.(1.54%)I.pentagona S.K.Chen,Y.X.Feng et C.F.Liang(1.23%)I.kudingcha C.J.Tseng(0.96%)I.centrochinesis X.Y.Hu(0.69%)the shoots of Ilex kudingcha C.J.Tseng(0.28%).The contents of oleanolic acid from 6 species of Kuding tea in Aquifoliaceae were as follows:I.latifolia Thunb.(0.22%)I.huoshanesis Y.H.He(0.18%)I.cornuta Lindl.(0.17%)I.pentagona S.K.Chen,Y.X.Feng et C.F.Liang(0.14%)I.kudingcha C.J.Tseng(0.12%)I.centrochinesis X.Y.Hu(0.1%)the shoots of Ilex kudingcha C.J.Tseng(0).[Conclusion] The determination results were favorable for the selection of suitable materials for the processing of ursolic acid.

Ursolic acid

Phosphoric acid

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Profiling of gut microbial dysbiosis in adults with myeloid leukemia

FEBS Open Bio (2021)

Dandan Yu Xiao-Min Yu Aifang Ye Chunquan Xu Xiaolong Li

Dysregulation of gut microbiota is implicated in the pathogenesis of various diseases, including metabolic diseases, inflammatory diseases, and cancer. To date, the link between gut microbiota and myeloid leukemia (ML) remains largely unelucidated. Herein, a total of 29 patients with acute myeloid leukemia (AML), 17 patients with chronic myeloid leukemia (CML), and 33 healthy subjects were enrolled, and gut microbiota were profiled via Illumina sequencing of the 16S rRNA. We evaluated the correlation between ML and gut microbiota. The microbial α‐diversity and β‐diversity exhibited significant differences between ML patients and healthy controls (HCs). Compared to healthy subjects, we found that at the phylum level, the relative abundance of Actinobacteria , Acidobacteria , and Chloroflexi was increased, while that of Tenericutes was decreased. Correspondingly, at the genus level in ML, Streptococcus were increased, especially in AML patients, while Megamonas (P = 0.02), Lachnospiraceae NC2004 group, and Prevotella 9 (P = 0.007) were decreased. Moreover, ML‐enriched species, including Sphingomonas , Lysobacyer , Helicobacter , Lactobacillus , Enterococcus, and Clostridium sensu stricto 1, were identified. Our results indicate that the gut microbiota was altered in ML patients compared to that of healthy subjects, which could contribute to the elucidation of microbiota‐related pathogenesis of ML, and the development of novel therapeutic strategies in the treatment of ML.

Lachnospiraceae

Prevotella

Dysbiosis

Pathogenesis

10.1002/2211-5463.13193

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Supplementary Data Figure S1 to S6, and Table S3 and S4. from Tie2–FGFR1 Interaction Induces Adaptive PI3K Inhibitor Resistance by Upregulating Aurora A/PLK1/CDK1 Signaling in Glioblastoma

Xiaolong Li Emmanuel Martínez-Ledesma Chen Zhang Feng Gao Siyuan Zheng

<p>This file contains supplementary Figure S1 to S6, and Table S3 and S4.</p>

PLK1

10.1158/0008-5472.22421865.v1

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Table S2 from Tie2–FGFR1 Interaction Induces Adaptive PI3K Inhibitor Resistance by Upregulating Aurora A/PLK1/CDK1 Signaling in Glioblastoma

Xiaolong Li Emmanuel Martínez-Ledesma Chen Zhang Feng Gao Siyuan Zheng

<p>Compounds map of high-throughput drug screening</p>

PLK1

Table (database)

10.1158/0008-5472.22421859

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An integrative analysis of ASCL1 in breast cancer and inhibition of ASCL1 increases paclitaxel sensitivity by activating ferroptosis via the CREB1/GPX4 axis

Frontiers in Immunology (2025)

Xiaolu Yang Yilun Li Yaqi Peng Chang Yuan Bei He

Objective Our previous study found that Achaete-scute complex homolog 1 (ASCL1) is involved in classifying BC subtypes with different prognostic and pathological characteristics. However, the biological role of ASCL1 in BC still remains largely unexplored. This study aims to elucidate the function of ASCL1 in BC using bioinformatics analyses, as well as in vitro and in vivo experimental approaches. Methods Data from the TCGA, GEO, and Human Protein Atlas databases were utilized to evaluate ASCL1 expression in BC and its association with patient prognosis. Genetic alterations in ASCL1 were assessed through the COSMIC and cBioPortal databases, while the TIMER2.0 database provided insights into the relationship between ASCL1 expression and key gene mutations in BC. The GDSC database was used to examine correlations between ASCL1 levels and sensitivity to standard chemotherapeutic agents. Associations between ASCL1 expression and cytokines, immunomodulatory factors, MHC molecules, and receptors were analyzed using Pearson and Spearman correlation methods. The TIP database was employed to investigate the connection between ASCL1 expression and immunoreactivity scores, and six computational approaches were applied to evaluate immune cell infiltration. Functional assays were conducted on BC cell lines MCF-7 and MDA-MB-231, and nude mouse models were used for in vivo studies. Results ASCL1 was found to be upregulated in BC and correlated with unfavorable prognosis and mutations in key oncogenes. Its expression was linked to immunomodulatory factors, immune cell infiltration, and immunoreactivity scores in the tumor microenvironment. Additionally, ASCL1 influenced tumor immune dynamics and chemosensitivity in BC. Overexpression of ASCL1 enhanced BC cell proliferation, migration and invasion, while its knockdown had the opposite effect. Notably, inhibition of ASCL1 increased BC cell sensitivity to paclitaxel both in vitro and in vivo . In addition, inhibition of ASCL1 activated ferroptosis in BC, including altered mitochondrial morphology, increased MDA and ROS levels, decreased GSH levels and reduced GSH/GSSG ratio. Mechanistically, inhibition of ASCL1 decreases the phosphorylation of CREB1, thus reducing the expression of GPX4. In summary, inhibition of ASCL1 increases paclitaxel sensitivity by activating ferroptosis via the CREB1/GPX4 axis. Conclusions ASCL1 exerts oncogenic effects in BC and represents a potential therapeutic target for intervention.

10.3389/fimmu.2025.1546794

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A new prediction nomogram of non-sentinel lymph node metastasis in cT1-2 breast cancer patients with positive sentinel lymph nodes

Scientific Reports (2024)

Yang Liu Xueyi Zhao Lixian Yang Chang Yan Congbo Cao

Abstract We aimed to analyze the risk factors and construct a new nomogram to predict non-sentinel lymph node (NSLN) metastasis for cT1-2 breast cancer patients with positivity after sentinel lymph node biopsy (SLNB). A total of 830 breast cancer patients who underwent surgery between 2016 and 2021 at multi-center were included in the retrospective analysis. Patients were divided into training ( n = 410), internal validation ( n = 298), and external validation cohorts ( n = 122) based on periods and centers. A nomogram-based prediction model for the risk of NSLN metastasis was constructed by incorporating independent predictors of NSLN metastasis identified through univariate and multivariate logistic regression analyses in the training cohort and then validated by validation cohorts. The multivariate logistic regression analysis revealed that the number of positive sentinel lymph nodes (SLNs) ( P < 0.001), the proportion of positive SLNs ( P = 0.029), lymph-vascular invasion ( P = 0.029), perineural invasion ( P = 0.023), and estrogen receptor (ER) status ( P = 0.034) were independent risk factors for NSLN metastasis. The area under the receiver operating characteristics curve (AUC) value of this model was 0.730 (95% CI 0.676–0.785) for the training, 0.701 (95% CI 0.630–0.773) for internal validation, and 0.813 (95% CI 0.734–0.891) for external validation cohorts. Decision curve analysis also showed that the model could be effectively applied in clinical practice. The proposed nomogram estimated the likelihood of positive NSLNs and assisted the surgeon in deciding whether to perform further axillary lymph node dissection (ALND) and avoid non-essential ALND as well as postoperative complications.

Nomogram

Perineural invasion

10.1038/s41598-024-60198-0

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Sheep fecal transplantation affects growth performance in mouse models by altering gut microbiota

Journal of Animal Science (2022)

Jiangbo Cheng Xiaoxue Zhang Deyin Zhang Yukun Zhang Xiaolong Li

Animal growth traits are important and complex traits that determine the productivity of animal husbandry. There are many factors that affect growth traits, among which diet digestion is the key factor. In the process of animal digestion and absorption, the role of gastrointestinal microbes is essential. In this study, we transplanted two groups of sheep intestinal microorganisms with different body weights into the intestines of mice of the same age to observe the effect of fecal bacteria transplantation on the growth characteristics of the mouse model. The results showed that receiving fecal microbiota transplantation (FMT) had an effect on the growth traits of recipient mice (P < 0.05). Interestingly, only mice receiving high-weight donor microorganisms showed differences. Use 16S rDNA sequencing technology to analyze the stool microorganisms of sheep and mice. The microbial analysis of mouse feces showed that receiving FMT could improve the diversity and richness of microorganisms (P < 0.05), and the microbial composition of mouse feces receiving low-weight donor microorganisms was similar to that of the control group, which was consistent with the change trend of growth traits. The feces of high-weight sheep may have higher colonization ability. The same five biomarkers were identified in the donor and recipient, all belonging to Firmicutes, and were positively correlated with the body weight of mice at each stage. These results suggest that FMT affects the growth traits of receptors by remodeling their gut microflora.The rate of development of growth traits determines the productivity of sheep. Shortening the fattening cycle of sheep can effectively improve economic efficiency. Gut microbes have important effects on host growth traits. In this study, the method of fecal microbiota transplantation (FMT) was used to transplant the intestinal microbes of sheep into the mouse model, and the changes of the growth traits and intestinal microbes of the mice were observed. The results suggest that FMT can effectively affect growth traits by altering recipient gut microbes. This provides a new idea for the regulation of sheep growth traits.

Digestion

10.1093/jas/skac303

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Hapten Synthesis and Identification of Tetracycline

Xiaolong Li

A new synthesis way of hapten for tetracycline base on the formation of its diazo derivatives with acidification,extraction by ethyl acetate.Then,happen TC was covalently attached through its carboxylic acid moiety to the carrier pro-tein(BSA,OVA)by means of the active ester method for preparing immungens and coating antigen,the titer of serum were 1﹕70 000 and 1﹕32 000,respectively.The purpose of this artificial is to provide an effective way to preparate haptens for tetracycline.This study laid a well foundation for the control for the control of tetracycline by ELISA method.

Hapten

Moiety

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Comparative genomics analysis provides insights into evolution and stress responses of Lhcb genes in Rosaceae fruit crops

BMC Plant Biology (2023)

Xiaolong Li Zeyu Jiang Chaofan Zhang Kefan Cai Hui Wang

Light-harvesting chlorophyll a/b b evelopment of higher plants and in response to abiotic stress. Previous works has demonstrated that that Lhcb genes were involved in the phytochrome regulation and responded to the different light and temperature conditions in Poaceae (such as maize). However, the evolution and functions of Lhcb genes remains poorly characterized in important Rosaceae species.In this investigation, we conducted a genome-wide analysis and identified a total of 212 Lhcb genes across nine Rosaceae species. Specifically, we found 23 Lhcb genes in Fragaria vesca, 20 in Prunus armeniaca, 33 in Malus domestica 'Gala', 21 in Prunus persica, 33 in Rosa chinensis, 29 in Pyrus bretschneideri, 18 in Rubus occidentalis, 20 in Prunus mume, and 15 in Prunus salicina. Phylogenetic analysis revealed that the Lhcb gene family could be classified into seven major subfamilies, with members of each subfamily sharing similar conserved motifs. And, the functions of each subfamily was predicted based on the previous reports from other species. The Lhcb proteins were highly conserved within their respective subfamilies, suggesting similar functions. Interestingly, we observed similar peaks in Ks values (0.1-0.2) for Lhcb genes in apple and pear, indicating a recent whole genome duplication event (about 30 to 45 million years ago). Additionally, a few Lhcb genes underwent tandem duplication and were located across all chromosomes of nine species of Rosaceae. Furthermore, the analysis of the cis-acting elements in the 2000 bp promoter region upstream of the pear Lhcb gene revealed four main categories: light response correlation, stress response correlation, hormone response correlation, and plant growth. Quantitative expression analysis demonstrated that Lhcb genes exhibited tissue-specific expression patterns and responded differently to low-temperature stress in Rosaceae species.These findings shed light on the evolution and phylogeny of Lhcb genes in Rosaceae and highlight the critical role of Lhcb in pear's response to low temperatures. The results obtained provide valuable insights for further investigations into the functions of Lhcb genes in Rosaceae, and these functional genes will be used for further fruit tree breeding and improvement to cope with the current climate changes.

Subfamily

Malus

Prunus armeniaca

Rubus

10.1186/s12870-023-04438-x

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Effects of circFOXO3 on the Proliferation and Invasion of Liver Cancer Cells by Regulating PI3K/Akt Pathway

Contrast Media & Molecular Imaging (2022)

Siyu Liu Minzi Yao Xiaolong Li Yumei Liu Cuiling Xie

Objective . Hepatocellular carcinoma is a malignant disease occurring in the liver and is one of the main causes of death in cancer patients. Tumor cells are the main components of tumors and have a strong proliferative capacity. They are easily transferred to other parts of the body and can produce harmful substances that destroy the normal organ structure and endanger human life and health. In this study, we investigate the effect of circFOXO3 on the proliferation and invasion of hepatocellular carcinoma cells and its possible mechanism. Methods . Human hepatocellular carcinoma cells BEL‐7404, Hep G2, Hep 3B2.1–7, HuH‐7, Li‐7, and human normal hepatocytes HHL‐5 were selected, and the expression level of circFOXO3 in the cell lines was determined by qRT‐PCR. The cell line with low circFOXO3 expression level (HuH‐7 cells) was used for follow‐up experiments. HuH‐7 liver cancer cells were divided into the control group (normal cultured), circFOXO3‐NC group (transfected with circFOXO3 negative control), circFOXO3 mimic group (transfected with circFOXO3 mimic), PI3K activator group (20 μ mol/L PI3K activator 740Y‐P), and circFOXO3 mimic + PI3K activator group (transfected with circFOXO3 mimic + treated with PI3K activator 740Y‐P). The qRT‐PCR method was used to determine the expression level of circFOXO3 in HuH‐7 liver cancer cells in each group, WB was used to detect the expression of apoptosis, invasion, and phosphoinositide 3‐kinase/protein kinase B (PI3K/Akt) pathway related proteins in HuH‐7 liver cancer cells in each group, the CCK‐8 method was used to determine the viability of HuH‐7 liver cancer cells in each group, flow cytometry was used to determine the apoptotic ability of HuH‐7 liver cancer cells in each group, the transwell chamber experiment was used to determine the invasion ability of HuH‐7 liver cancer cells in each group, and the scratch test was used to determine the migration ability of HuH‐7 liver cancer cells in each group. Results . circFOXO3 showed low expression in liver cancer cells; compared with the control group, the circFOXO3 expression and apoptosis rate of HuH‐7 liver cancer cells in the circFOXO3 mimic group were significantly increased ( P < 0.05) and the PI3K/Akt pathway‐related protein expression, cell viability, invasion, and migration abilities were significantly reduced ( P < 0.05); the apoptosis rate of HuH‐7 liver cancer cells in the PI3K activator group was significantly reduced ( P < 0.05) and the PI3K/Akt pathway related protein expression, cell viability, invasion and migration abilities were significantly increased ( P < 0.05). Compared with the circFOXO3 mimic group, the apoptosis rate of HuH‐7 liver cancer cells in the circFOXO3 mimic + PI3K activator group was significantly reduced ( P < 0.05) and the PI3K/Akt pathway‐related protein expression, cell viability, invasion and migration abilities were significantly increased ( P < 0.05). Conclusion . Highly expressed circFOXO3 can inhibit the proliferation and invasion of HuH‐7 liver cancer cells, which may be achieved by inhibiting the PI3K/Akt pathway.

Liver Cancer

10.1155/2022/2109908

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