Platelets with high hemostatic activity play a key role during percutaneous coronary interventions (PCI), and in recent years, mean platelet volume (MPV) has been looked upon as a crucial indicator of platelet reactivity. Thus, MPV may emerge as a potential gauge for the measurement of major adverse cardiac event (MACE) risks in PCI patients. This study aimed to conduct a meta-analysis illustrating the association between MPV and long-term MACE in PCI. The Cochrane Library, Pubmed, EMBASE, Ovid MEDLINE, and BIOSIS databases were used to search for relevant studies from their inception to 30 June 2019. All studies reporting incidences of MACE and MPV in PCI patients were retained. Data extraction was performed by three independent reviewers. A total of 33 studies were included in this meta-analysis. The results indicated that patients with MACE had a significantly larger MPV than those without, with an unstandardized mean difference (USMD) of 0.29 fL (95% CI, 0.04–0.54). The USMD of MPV in deceased patients was 0.39 fL (95% CI, 0.09–0.68). The results also indicated that patients with larger MPV were at greater risks of having MACE and higher incidence of mortality than those with smaller MPV, with a pooled risk ratio of 1.81 (95% CI, 1.29–2.55) and 2.34 (95% CI, 1.52–3.60), respectively. These findings indicate a significant association between larger MPV and MACE in PCI patients. Consequently, MPV, an easily accessible indicator, might be helpful in PCI patients’ risk assessment and stratification.
FasL-DC were cultured with C57BL/6 mice hematopoietic stem cell grafts, and the modified stem cell grafts were used in a C57BL/6 to BALB/c mice GVHD model system H-2~(b)→H-2~(d), or FasL-DC were directly administered intravenously or intraperitoneally. The GVHD clinical manifestations were observed. Lethally irradiated recipients receiving donor hematopoietic stem cell grafts pretreated with FasL-DC in vitro did not develop lethal GVHD. In contrast, the other groups displayed all clinical signs of acute GVHD after transplantation. FasL-DC administered in vitro to pretreated donor haematopoietic stem cell grafts are more effective than those administered IV or IP in protecting against GVHD.
Diabetes increases the vulnerability of the heart to ischemic injury. Recent advances have shown that necroptosis is crucially involved in myocardial ischemia/reperfusion (I/R) injury. Our objectives were thus to extend our knowledge to whether and how necroptosis renders diabetic hearts more vulnerable to I/R injury, which involves activation of Ca 2+ -calmodulin-dependent protein kinase (CaMKII) by receptor-interacting protein 3 (RIP3). In this study, diabetes was induced by streptozotocin (40mg/kg, i.v.) and after 6 weeks, anesthetized open-chest diabetic and age-matched control C57 mice were subjected to 30-min regional ischemia (occlusion of LAD coronary artery) followed by 4-h reperfusion. Diabetic animals manifested significantly greater cardiac injury than their littermate controls. Notably, diabetic hearts were sensitized to I/R-induced myocardial necrosis, as evidenced by rise in lactate dehydrogenase (LDH) release and Evans blue dye (EBD) penetration. Notably, compared with control mice, the activation pattern of CaMKII in diabetic hearts subjected to I/R is altered by earlier advanced (60 min after reperfusion) phosphorylation and enhanced oxidation (21.5% increase). Then we proved that inhibition of CaMKII in early stage of I/R could effectively reduce myocardial necroptosis. Further, RIP3 expression and RIP3-CaMKII interaction are markedly enhanced in diabetic hearts during I/R. Inhibition of RIP3 markedly suppresses CaMKII activation and reduces necroptosis. Collectively, these findings identify enhanced RIP3-CaMKII pathway as a key regulator that increases myocardial necroptosis in diabetic I/R hearts.
Angiotensin receptor neprilysin inhibitor (ARNI) sacubitril-valsartan has been recommended as one of the first-line therapies in heart failure with reduced ejection fraction. However, whether ARNI could benefit patients with ST-segment elevation myocardial infarction (STEMI) by improving left ventricular (LV) remodeling remains unknown. The primary objective of the PERI-STEMI trial is to assess whether sacubitril-valsartan is more effective in preventing adverse LV remodeling for patients with STEMI than enalapril.
Abstract Over the past decade, organoids have emerged as a prevalent and promising research tool, mirroring the physiological architecture of the human body. However, as the field advances, the traditional use of animal or tumor-derived extracellular matrix (ECM) as scaffolds has become increasingly inadequate. This shift has led to a focus on developing synthetic scaffolds, particularly hydrogels, that more accurately mimic three-dimensional (3D) tissue structures and dynamics in vitro. The ECM–cell interaction is crucial for organoid growth, necessitating hydrogels that meet organoid-specific requirements through modifiable physical and compositional properties. Advanced composite hydrogels have been engineered to more effectively replicate in vivo conditions, offering a more accurate representation of human organs compared to traditional matrices. This review explores the evolution and current uses of decellularized ECM scaffolds, emphasizing the application of decellularized ECM hydrogels in organoid culture. It also explores the fabrication of composite hydrogels and the prospects for their future use in organoid systems.
Objective: To study the effects of vitamin A deficiency (VAD) on brain development, learning and memory ability in young rats. Methods: A model of VAD in female rats and the offsprings were established. The growth and development, learning and memory ability, protein content, cell proliferative cycle of cortex and hippocampus, and hippocampus cell ultrastructure changes under electron microscopy of young rats were detected. Results: (1) The weights gain in VAD rats were decreased compared with the control. (2) The learning and memory scores in VAD rats was significantly lower than those of the control. (3) The protein content of cortex and hippocampus in VAD rats were significantly lower than those of the control. (4) The cell proliferative index (PI) of cortex and hippocampus in VAD rats were higher than those of the control. (5) The synapses between hippocampus pyramidal cells and the small bubbles in synapses in VAD rats were significantly decreased compared with the control. The astrocytes of hippocampus in VAD rats were significantly increased. Degeneration was observed in many astrocytes in VAD rats. Conclusion: VAD can affect the brain development and the ability of learning and memory in young rats.
An ideal arterial pressure monitoring system for perioperative care is required to be accurate, noninvasive, continuous, and risk free. Although several continuous noninvasive arterial pressures (CNAP) are determined on the finger, a new wrist CNAP monitoring system was developed. This prospective study was designed as a randomized-controlled trial to assess its validity in comparison with invasive arterial pressure (IAP) monitoring.Sixty patients undergoing elective surgery under general anesthesia were enrolled. One-side arm in each patient was selected randomly to assess the new monitoring system placed on the wrist; an arterial catheter for IAP was inserted at the radial artery in the contralateral arm. The Bland-Altman method for repeated measurements was used to assess agreement between measurement methods by the levels of agreement according to the American Association for the Advancement of Medical Instrumentation standards.A total of 6600 valid pressure readings were obtained from 60 patients, including 3000 beat data and 3600 pulse wave data, respectively. The mean differences in wrist CNAP versus IAP for beat data were as follows: systolic arterial pressure (SAP): -2.09±5.39 mmHg; mean arterial pressure (MAP): 0.27±3.64 mmHg; diastolic arterial pressure (DAP): and 2.63±6.44 mmHg. For pulse wave data, the mean difference (SD) values were as follows: SAP: -2.06±6.51 mmHg; MAP: 0.50±4.36 mmHg; and DAP: 3.06±6.81 mmHg. Percentage errors were less than 28.3%. Levels of agreements were detected to be -12.65-8.47 for SAP, -6.86-7.40 for MAP, and -9.99-15.26 for DAP.The new wrist CNAP monitoring system showed an acceptable agreement and interchangeability with the IAP.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)