Yonggang Li

Jinzhou Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Kazuyoshi Ikuta

Osaka University

Wei Zhao

Jinzhou Medical University

Xiaoli Tao

Jinzhou Medical University

Shoutaro Tsuji

Kanagawa Cancer Center

Fu‐Sheng Wang

Chinese PLA General Hospital

Zheng Zhang

Shanxi Jincheng Anthracite Mining Group (China)

Malinee Chittaganpitch

Department of Medical Sciences

Madiha S. Ibrahim

Damanhour University

Jiranan Warachit

Ministry of Public Health

Pathom Sawanpanyalert

Ministry of Public Health

Cooperative Institutions

Chinese Academy of Sciences

433

Institute of High Energy Physics

340

University of Chinese Academy of Sciences

160

Shanghai Jiao Tong University

100

Peking University

Sun Yat-sen University

Chinese Academy of Medical Sciences & Peking Union Medical College

Nanjing University

Huazhong University of Science and Technology

Tsinghua University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells

Nature Cell Biology (2024)

Huafeng Zhang Jincheng Liu Yuan Wu Qian Zhang Xiao Luo

10.1038/s41556-024-01503-x

Cite

Citations (9)

Evaluation of immunologic enhancement mediated by a polysaccharide isolated from the fruit of Physalis alkekengi L. var. francheti (Mast.) Makino

Journal of Medicinal Plants Research (2011)

Yonggang Li Shuying Han Zhaohua Hou Yali Liu Jinbo Fang

The study was to investigate the immunologic enhancement mediated by a polysaccharide (designated PPSB) purified from the mature fruits of Physalis alkekengi L. var. francheti (Mast.) Makino. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone, OVA/PPSB, OVA/QuilA, and saline two times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze humoral and cellular immunity. Delayed-type hypersensitivity (DTH) response was further detected. Moreover, toxicity of PPSB was evaluated by haemolytic activity and acute toxicity assay. The results showed that PPSB significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum, the concanavalin (ConA), lipopolysaccharide (LPS), OVA-induced splenocyte proliferation, proportions of CD4+ and CD8+ T lymphocytes in spleen, DTH response when compared with OVA-injected mice (P < 0.05 or P < 0.01). Toxicity assay indicated PPSB was non-toxic. Therefore, PPSB mediated both humoral immunity and cellular immunity, and it was non-toxic. Thus, PPSB should be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine. Key words: Herbal medicine, polysaccharide, humoral response, cellular response, adjuvant.

Humoral immunity

Physalis

Splenocyte

Cellular immunity

10.5897/jmpr.9000627

Cite

Citations (7)

Analysis of the Intestinal Microbial Community Altered During Rotavirus Infection in Suckling Mice

Research Square (Research Square) (2021)

Wei Zhao Xiaoli Tao Meiling Yu Mei-hui Cheng Changcheng Liu

Abstract Background： Rotavirus (RV) is a principal cause of diarrhea. However, there is a limited understanding regarding alteration of the gut microbial community structure and abundance during RV infection. This study was to characterize any potential associations between RV infection and the intestinal microbiota. Methods: Suckling mice were divided into normal group (NC) and infected group (RV) randomly. All of the suckling mice were euthanized four days post-RV infection. The virus titer was counted as fluorescent focus assay (FFA), and viral load was quantified by QPCR. Five sucking mice were randomly selected from each RV group and NC group for sample collection and pathological analysis. All of the suckling mice mixed intestinal contents of the colon and rectum were collected. To investigate the detailed relationship between RV infection and intestinal microbiota, the composition and distribution of intestinal microbiota from suckling mice were first analyzed using 16S rRNA sequencing technology. Results: The results of the pathological characteristics showed that vacuolar degeneration, vasodilation, hyperemia, and destruction of the intestinal epithelium were apparent in the RV group. Representative genera from Lactobacillus and Fusobacterium were enriched in the NC group, while the Enterococcus and Escherichia/Shigella genera were enriched in the RV group. Helicobacter, Alloprevotrlla, Brevundimonas, Paenibacillus, and Parabacteroides were completely undetectable in the RV group. The predicted intestinal flora metabolic function results showed that “carbohydrate metabolism” and “lipid metabolism” pathways were significantly enriched within the NC group. A significant difference has been observed in the gut microbiota composition between the two groups. Conclusions: Our results demonstrated a significant difference in the gut microbiota composition in RV-infected suckling mice as compared to the RV un-infected suckling mice group. This work may provide meaningful information regarding the bacterial genera changed during RV infection. Moreover, the changes in these bacteria may affect the replication of RV and contribute to the pathogenesis of RV infection.

Roseburia

Eubacterium

Intestinal epithelium

10.21203/rs.3.rs-696842/v1

Cite

Citations (0)

Characterization of a G9 group A rotavirus reassortant strain detected in Jinzhou, China, in 2018–2019

Archives of Virology (2020)

Ying Lu Hui Li Weiwei Li Xiaofang Wang Xiaoli Tao

Reassortment

Strain (injury)

Molecular Epidemiology

Group A

10.1007/s00705-020-04563-0

Cite

Citations (7)

DETECT CRYPTIC CHROMOSOMAL REARRANGEMENT BY OPTICAL GENOME MAPPING (OGM) IN INFERTILE POPULATION

Fertility and Sterility (2022)

Yunxiu Li Yan Xu Jinghui Yang Yonggang Li Yanwen Xu

Chromosomal rearrangement

10.1016/j.fertnstert.2022.08.714

Cite

Citations (0)

Characterization of two anti-dengue human monoclonal antibodies prepared from PBMCs of patients with dengue illness in Thailand

Acta Virologica (2016)

Ziqiang Li Atsushi Yamashita Norihito Kawashita Tadahiro Sasaki Yang Pan

Th e global spread of the four dengue virus (DENV) serotypes (dengue-1 to -4) has made this virus a major and growing public health concern.Generally, pre-existing neutralizing antibodies derived from primary infection play a signifi cant role in protecting against subsequent infection with the same serotype.By contrast, these pre-existing antibodies are believed to mediate a non-protective response to subsequent heterotypic DENV infections, leading to the onset of dengue illness.In this study, two monoclonal antibodies prepared by using peripheral blood mononuclear cells (PBMCs) from patients with dengue fever were characterized.Epitope mapping revealed that amino acid residues 254-278 in domain II of the viral envelope protein E were the target region of these antibodies.A database search revealed that certain sequences in this epitope region showed high conservation among the four serotypes of DENV.Th ese two human monoclonal antibodies could neutralize DENV-2,-4 more eff ectively than DENV-1,-3.Th e amino acid sequences could not explain this diff erence in neutralizing activity.However, the 3D structure results showed that amino acid 274 could be the critical residue for the diff erence in neutralization.Th ese results may provide basic information for the development of a dengue vaccine.

Dengue vaccine

Flavivirus

Antibody-dependent enhancement

10.4149/av_2016_02_166

Cite

Citations (0)

Enhancement of human immunodeficiency virus type 1 infectivity by replacing the region including Env derived from defective particles with an ability to form particle-mediated syncytia in CD4+T cells

Microbes and Infection (2004)

Masanobu Kinomoto Tetsu Mukai Yonggang Li Yukie Iwabu Jiranan Warachit

Syncytium

Infectivity

Provirus

clone (Java method)

Cell fusion

10.1016/j.micinf.2004.05.003

Cite

Citations (3)

Adjuvant effect of saponins isolated from the sepal of Physalis alkekengi L. var. francheti (Mast.) Makino fruit on the immune responses to ovalbumin in mice

Journal of Medicinal Plants Research (2011)

Yali Liu Shuying Han Yanshu Zhu Yonggang Li Zhu XiaoJuan

Saponins (designated SP) were isolated and purified from the sepals of the mature fruit ofPhysalis alkekengi L. var. francheti (Mast.) Makino which is a traditional Chinese medicine herb. Immunologic enhancement of the SP was evaluated in this paper. Male ICR mice were immunized subcutaneously with ovalbumin (OVA) alone or with OVA containing SP in doses of 0.63, 1.25, 2.50 mg/kg body weight (BW), or QuilA in doses of 0.5, 1.0 mg/kg BW 2 times at 14-day interval. The mice were sacrificed two weeks after the last immunization to analyze humoral and cellular immunity. Delayed-type hypersensitivity (DTH) response was further detected. The results showed that SP (1.25 mg/kg BW) significantly enhanced OVA-specific antibody titers (IgG, IgG1, IgG2b) in serum, the concanavalin (ConA), lipopolysaccharide (LPS), OVA-induced splenocyte proliferation, proportions of CD4+ and CD8+ T lymphocytes in spleen, delayed-type hypersensitivity (DTH) response when compared with OVA-injected mice (P<0.05 or P<0.01). More importantly, haemolytic activity assay indicated SP had lower haemolytic effect than QuilA. Therefore, SP evoked both humoral immunity and cellular immunity, and can be considered as a promising adjuvant eliciting both Th1 and Th2 responses to improve the efficacy of vaccine. Key words: Herbal medicine, saponin, humoral response, cellular response, adjuvant.

Humoral immunity

Splenocyte

Cellular immunity

Physalis

10.5897/jmpr.9000045

Cite

Citations (2)

Production of polyclonal antibody to a recombinant non-structural protein Nsp1a of human astrovirus

Journal of Virological Methods (2014)

Chang Liu Wenhui Liu Li-li Kan Xin Li Yonggang Li

Polyclonal antibodies

Astrovirus

Immunofluorescence

10.1016/j.jviromet.2014.09.007

Cite

Citations (3)

Sharing Technology of the Tracking Telemetry and Command Software

Applied Mechanics and Materials (2014)

Yonggang Li Zhen Dong Xi Hao Sha Li Bing Guo Wang Yi

The software sharing is the development trend of tracking telemetry and command software. How to develop quickly the tracking and controlling tasks software to adapt to the current experimental situation, and to ensure software quality, is a challenging subject placed in the face of software personnel. Using software middleware and software component technology, we can solve the problems of Tracking and Controlling Network acrossing operating system, Tracking and Controlling Software acrossing program languages, software reuse and other issues.

Tracking (education)

Package development process

10.4028/www.scientific.net/amm.644-650.1245

Cite

Citations (0)