Lu Liu

Despite significant developments in optical imaging of superoxide anion (O2•–) as the preliminary reactive oxygen species, novel visualizing strategies that offer ultrahigh sensitivity are still imperative. This is mainly because intrinsic concentrations of O2•– are extremely low in living systems. Herein, we present the rational design and construction of a new polymer nanoprobe PCLA-O2•– for detecting O2•– based on chemiluminescence (CL) resonance energy transfer without an external excitation source. Structurally, PCLA-O2•– contains two moieties linked covalently, namely imidazopyrazinone that is capable of CL triggered by O2•– as the energy donor and conjugated polymers with light-amplifying property as the energy acceptor. Experiment results demonstrate that PCLA-O2•– exhibits ultrahigh sensitivity at the picomole level, dramatically prolonged luminescence time, specificity, and excellent biocompatibility. Without exogenous stimulation, this probe for the first time in situ visualizes O2•– level differences between normal and tumor tissues of mice. These exceptional features ensure that PCLA-O2•– as a self-luminescing probe is an alternative in vivo imaging approach for ultralow level O2•–.

Nanoprobe

10.1021/jacs.5b11784

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Controllable accumulation of conjugated polymer nanoparticles on the surface of adhesive bacteria

Colloids and Surfaces A Physicochemical and Engineering Aspects (2020)

Lu Liu Xiaoyu Wang Dongdong Ban Shuxian Zhu Lidong Li

Sterilization

10.1016/j.colsurfa.2020.124569

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Two-photon fluorescence imaging of sialylated glycans in vivo based on a sialic acid imprinted conjugated polymer nanoprobe

Chemical Communications (2016)

Wei Zhang Junqing Kang Ping Li Lu Liu Hui Wang

We have designed and synthesized a sialic acid (SA)-imprinted conjugated polymer nanoprobe with two-photon fluorescence properties, which exhibits specific recognition ability to the target SA and has been used for monitoring sialylated glycan levels selectively in vivo.

Nanoprobe

Polysialic acid

Two-photon excitation microscopy

10.1039/c6cc08211d

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Citations (33)

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