This research is among the first few to examine the role of consumers’ power distance belief (PDB) in influencing the effect of nostalgic ad appeal. Findings demonstrate that the stronger effect of personal nostalgia (vs. historical) is attenuated among low-PDB (vs. high-PDB) consumers, and for highly (vs. lowly) visible products. When a joint moderating effect of PDB and product visibility is tested, for highly visible products, the stronger effect of personal vs. historical nostalgic ads is found among high-PDB consumers whereas it disappears among low-PDB counterparts. For lowly visible products, the stronger effect of personal vs. historical nostalgic ads remains unchanged across high- and low-PDB consumers. These findings have important implications to marketers/advertisers on how to effectively utilize nostalgic ad appeal.
Abusive supervision is closely related to the negative psychological reaction of employees. This paper mainly discusses the influence mechanism of the abusive supervision on the job insecurity. A moderated mediation model is put forward. Study used questionnaire to investigate the enterprise staff. The results showed: 1) Abusive supervision has a direct impact on job insecurity, and also has indirect impact on job insecurity through the LMX; 2) The power distance has a moderated effect on the mediated role of LMX. To be specific, the impact of the Abusive supervision on job insecurity through the LMX decreased with the increase of the power distance. Therefore, the influence mechanism of abusive supervision on job insecurity is a moderated mediation model.
Major depression disorder is one of the most common psychiatric diseases. Recent evidence supports that environmental stress affects gene expression and promotes the pathological process of depression through epigenetic mechanisms. Three ten-eleven translocation (Tet) enzymes are epigenetic regulators of gene expression that promote 5-hydroxymethylcytosine (5hmC) modification of genes. Here, we show that the loss of Tet2 can induce depression-like phenotypes in mice. Paradoxically, using the paradigms of chronic stress, such as chronic mild stress and chronic social defeat stress, we found that depressive behaviors were associated with increased Tet2 expression but decreased global 5hmC level in hippocampus. We examined the genome-wide 5hmC profile in the hippocampus of Tet2 knockout mice and identified 651 dynamically hydroxymethylated regions, some of which overlapped with known depression-associated loci. We further showed that chronic stress could induce the abnormal nuclear translocation of Tet2 protein from cytosol. Through Tet2 immunoprecipitation and mass spectrum analyses, we identified a cellular trafficking protein, Abelson helper integration site-1 (Ahi1), which could interact with Tet2 protein. Ahi1 knockout or knockdown caused the accumulation of Tet2 in cytosol. The reduction of Ahi1 protein under chronic stress explained the abnormal Ahi1-dependent nuclear translocation of Tet2. These findings together provide the evidence for a critical role of modulating Tet2 nuclear translocation in regulating stress response.
The prevention and treatment of staphylococcus aureus septicemia is one of the thorniest problems in modern medicine. However, as the underlying pathogenesis of sepsis is still unclear, there is currently no golden standard for clinical diagnosis. In this study, we used GSE33341 dataset for differentially expressed gene (DEG) analysis and screened out 857 differentially expressed genes associated with staphylococcus aureus infection. The module having the highest correlation with clinical features of sepsis was screened by weighted gene co-expression network analysis (WGCNA). The genes in the selected module and the differentially expressed genes were represented in Venn diagram, and 59 pathogenic genes at the intersection were obtained. GO and KEGG analysis showed that these genes were mainly related to aerobic respiration, cellular stress response, mitochondrial electron transport, mitochondrial transport, oxidative phosphorylation. Kaplan-Meier was used to analyze the influence of the top 10 key genes on the prognosis of sepsis patients. The results showed that the high expression of NDUFA4, NDUFB3, COX7A2, ATP5J and COX7C was significantly correlated with the poor overall survival (OS) in patients with bacterial sepsis. These findings may potentially provide a reference for the diagnosis and treatment of bacterial septicemia.
Hirschsprung's disease (HSCR) is a neural crest disease that results from the failure of enteric neural crest cells (ENCCs) to migrate to the corresponding intestinal segment. The RET gene, which regulates enteric neural crest cell proliferation and migration, is considered one of the main risk factors for HSCR and is commonly used to construct HSCR mouse models. The epigenetic mechanism of m6A modification is involved in HSCR. In this study, we analyzed the GEO database (GSE103070) for differentially expressed genes (DEGs) and focused on m6A-related genes. Comparing the RNA-seq data of Wide Type and RET Null, a total of 326 DEGs were identified, of which 245 genes were associated with m6A. According to the CIBERSORT analysis, the proportion of Memory B-cell in RET Null was significantly higher than that of Wide Type. Venn diagram analysis was used to identify key genes in the selected memory B-cell modules and DEGs associated with m6A. Enrichment analysis showed that seven genes were mainly involved in focal adhesion, HIV infection, actin cytoskeleton organization and regulation of binding. These findings could provide a theoretical basis for molecular mechanism studies of HSCR.
Diabetic nephropathy (DN) is a common complication of diabetes. Due to its complex pathogenesis, there is no effective treatment. M6A is a newly discovered epigenetic mechanism that may be involved in the development of diabetic nephropathy. In this study, we analyzed differentially expressed genes (DEG) in the GEO database (GSE96804) and paid attention to genes with m6A methylation. 623 DEGs in glomerular tissue were identified by comparing diabetic nephropathy with normal. Correlation analysis with 21 genes involved in m6A modification showed that 492 genes were associated with m6A methylation. According to the CIBERSORT algorithm, the infiltration of M1 macrophages in DN patients was significantly higher than that in normal samples. Weighted gene coexpression network analysis (WGCNA) was used to screen for the modules most correlated with the clinical features of M1 macrophages. The genes in the selected modules and 492 m6A-related DEGs were intersected by a Venn diagram, and 43 key genes were obtained. GO and KEGG analyses showed that these genes were mainly related to the positive regulation of protein aggregation and the transforming growth factor β receptor signaling pathway. According to a literature review, among the top 10 genes, HSPA1A, HSPA1B, CHI3L1, TYRO3 and PTH1R are markers in diabetic nephropathy, and their abnormal expression is associated with renal hypertrophy, proteinuria and glomerulosclerosis. These findings may provide evidence for the diagnosis and treatment of diabetic nephropathy.
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