Huifang Li

Xiamen University

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Todd Waldman

Georgetown University

Jung-Sik Kim

Georgetown University

David A. Solomon

University of Maiduguri

David H. Rowitch

University of Cambridge

Tim Forshew

Research Triangle Park Foundation

Barbara S. Paugh

Lentigen Technology (United States)

Theodore Nicolaides

NYU Langone Health

Rintaro Hashizume

Northwestern University

Suzanne J. Baker

St. Jude Children's Research Hospital

William A. Weiss

Neurological Surgery

Cooperative Institutions

Chinese Academy of Sciences

113

Xiamen University

China Agricultural University

Chinese Academy of Medical Sciences & Peking Union Medical College

Zhejiang University

Sichuan University

University of Chinese Academy of Sciences

Sun Yat-sen University

Shanghai Jiao Tong University

Poultry Research Institute

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Characterization and genomic analysis of a novel Pseudoalteromonas phage PS_L5

Marine Genomics (2023)

Xing Zhou Jie Gao Rongda Xiao Yifan Qiao Yuang Zhu

Pseudoalteromonas

ORFS

Lytic cycle

Myoviridae

Siphoviridae

Temperateness

10.1016/j.margen.2023.101069

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Citations (2)

Gram-Scale Preparation of Cannflavin A from Hemp (Cannabis sativa L.) and Its Inhibitory Effect on Tryptophan Catabolism Enzyme Kynurenine-3-Monooxygenase

Biology (2022)

Tess Puopolo Tanran Chang Chang Liu Huifang Li Xu Liu

Inhibitors targeting kynurenine-3-monooxygenase (KMO), an enzyme in the neurotoxic kynurenine pathway (KP), are potential therapeutics for KP metabolites-mediated neuroinflammatory and neurodegenerative disorders. Although phytochemicals from Cannabis (C. sativa L.) have been reported to show modulating effects on enzymes involved in the KP metabolism, the inhibitory effects of C. sativa compounds, including phytocannabinoids and non-phytocannabinoids (i.e., cannflavin A; CFA), on KMO remain unknown. Herein, CFA (purified from hemp aerial material at a gram-scale) and a series of phytocannabinoids were evaluated for their anti-KMO activity. CFA showed the most active inhibitory effect on KMO, which was comparable to the positive control Ro 61-8048 (IC50 = 29.4 vs. 5.1 μM, respectively). Furthermore, a molecular docking study depicted the molecular interactions between CFA and the KMO protein and a biophysical binding assay with surface plasmon resonance (SPR) technique revealed that CFA bound to the protein with a binding affinity of 4.1×10−5 M. A competitive SPR binding analysis suggested that CFA and Ro 61-8048 bind to the KMO protein in a competitive manner. Our findings show that C. sativa derived phytochemicals, including CFA, are potential KMO inhibitors, which provides insight into the development of therapeutics targeting the KP and its related pathological conditions.

Kynurenine pathway

10.3390/biology11101416

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Citations (9)

Microglial hexokinase 2 deficiency increases ATP generation through lipid metabolism leading to β-amyloid clearance

Nature Metabolism (2022)

Lige Leng Ziqi Yuan Rui‐Yuan Pan Xiao Su Han Wang

Adenosine triphosphate

Hexokinase

TREM2

Amyloid (mycology)

10.1038/s42255-022-00643-4

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Citations (82)

Genomic analyses reveal the origin of domestic ducks and identify different genetic underpinnings of wild ducks

Authorea (2020)

Rui Liu weiqing liu Enguang Rong Lizhi Lu Huifang Li

Domestic ducks are considered to have been tamed from the mallard or a descendant of the mallard and the spot-billed duck. Domestic ducks show remarkable phenotypic variation in morphology, physiology and behaviour. However, the molecular genetics of the origin and phenotypic variation of ducks are still poorly studied. Here, we present mallard and spot-billed genomes and perform whole-genome sequencing on eight domestic duck breeds and eight wild duck species. Surprisingly, analyses of these data support a model in which domestic ducks diverged from their closest wild lineage (mallard ducks and spot-billed ducks) at the last glacial period (LGP, 100-300 kilo years ago (Kyr)). The wild lineage further speciated into mallard ducks and spot-billed ducks approximately 70 Kyr, whereas the domestic lineage population decreased through the LGP. A scan of wild duck genomes compared with domestic duck genomes identified numerous loci that may have been affected by positive selection in ancestral wild ducks after their divergence from domestic lineages. Function analyses suggested that genes usually affecting organ development and energy metabolism may involve long-distance flight ability. Further selective sweep analyses identified two genes associated with egg production and three genes related to feeding modulation under selection in domestic ducks. These analyses unravel a distinct evolutionary pattern of ducks and two wild duck de novo genomes, thus providing a novel resource for speciation studies.

Lineage (genetic)

Anas

Phenotypic trait

10.22541/au.158447739.91925748

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Isolation and Characterization of a Novel Vibrio Phage vB_ValA_R15Z

Current Microbiology (2024)

Huifang Li Wanxuan Zhong Xinyu Zhang Rui Zhang Yunlan Yang

Lytic cycle

Vibrio alginolyticus

Vibrio vulnificus

10.1007/s00284-024-03736-3

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Viral control of biomass and diversity of bacterioplankton in the deep sea

Communications Biology (2020)

Rui Zhang Yanxia Li Wei Yan Yu Wang Lanlan Cai

Abstract Viral abundance in deep-sea environments is high. However, the biological, ecological and biogeochemical roles of viruses in the deep sea are under debate. In the present study, microcosm incubations of deep-sea bacterioplankton (2,000 m deep) with normal and reduced pressure of viral lysis were conducted in the western Pacific Ocean. We observed a negative effect of viruses on prokaryotic abundance, indicating the top-down control of bacterioplankton by virioplankton in the deep-sea. The decreased bacterial diversity and a different bacterial community structure with diluted viruses indicate that viruses are sustaining a diverse microbial community in deep-sea environments. Network analysis showed that relieving viral pressure decreased the complexity and clustering coefficients but increased the proportion of positive correlations for the potentially active bacterial community, which suggests that viruses impact deep-sea bacterioplankton interactions. Our study provides experimental evidences of the crucial role of viruses in microbial ecology and biogeochemistry in deep-sea ecosystems.

Bacterioplankton

Microcosm

Biogeochemistry

Biogeochemical Cycle

Marine ecosystem

10.1038/s42003-020-0974-5

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Citations (23)

A High-Throughput Screen with Isogenic PTEN+/+ and PTEN–/– Cells Identifies CID1340132 as a Novel Compound That Induces Apoptosis in PTEN and PIK3CA Mutant Human Cancer Cells

SLAS DISCOVERY (2011)

Huifang Li Adam B. Keeton Michele Vítolo Clinton Maddox Lynn Rasmussen

The PTEN tumor suppressor gene is one of the most commonly mutated genes in human cancer. Because inactivation of PTEN is a somatic event, PTEN mutations represent an important genetic difference between cancer cells and normal cells and therefore a potential anticancer drug target. However, it remains a substantial challenge to identify compounds that target loss-of-function events such as mutations of tumor suppressors. In an effort to identify small molecules that preferentially kill cells with mutations of PTEN, the authors developed and implemented a high-throughput, paired cell-based screen composed of parental HCT116 cells and their PTEN gene-targeted derivatives. From 138 758 compounds tested, two hits were identified, and one, N'-[(1-benzyl-1H-indol-3-yl)methylene]benzenesulfonohydrazide (CID1340132), was further studied using a variety of cell-based models, including HCT116, MCF10A, and HEC1A cells with targeted deletion of either their PTEN or PIK3CA genes. Preferential killing of PTEN and PIK3CA mutant cells was accompanied by DNA damage, inhibition of DNA synthesis, and apoptosis. Taken together, these data validate a cell-based screening approach for identifying lead compounds that target cells with specific tumor suppressor gene mutations and describe a novel compound with preferential killing activity toward PTEN and PIK3CA mutant cells.

Synthetic Lethality

10.1177/1087057110397357

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Citations (9)

4-vinylcyclohexene diepoxide induces premature ovarian insufficiency in rats by triggering the autophagy of granule cells through regulating miR-144

Journal of Reproductive Immunology (2023)

Qun Zhou Xin Jin Jiaxi Wang Huifang Li Lijuan Yang

Viability assay

10.1016/j.jri.2023.103928

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Citations (4)

Photocatalytic degradation of chlorsulfuron in water by Pt/WO_3 using simulated solar light

日本水処理生物学会誌. 別巻 = Journal Japan Biological Society of Water and Waste (2011)

Huifang Li Yingnan Yang Chao Zhao Zhenya Zhang

Degradation

Visible spectrum

Source

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The Effect of Diluent on the Release of Benzene Series from Nitrocellulose-Lacquered MDF

Atmosphere (2022)

Xiaolei Cui Junhong Ma Huifang Li

Because the surface of MDF is not aesthetically pleasing, it usually needs to be veneered and then painted, but such a board releases harmful VOCs, among which Benzene Series is the most harmful. Benzene and its series are a group of carcinogenic compounds. With the diluents of nitrocellulose (NC)-lacquered MDF as the research objects, the release of the Benzene Series was studied to provide a scientific basis for pollution control and a reference for eco-friendly paint production. The attenuant of NC paint, anhydrous ethanol, ethyl acetate and solvents mixed with different alcohol ester ratios were used as diluents in NC lacquer. Two kinds of wood-veneered MDF with different thickness (18 mm and 8 mm) were coated with NC lacquer and analyzed in the experiment. The gas was collected using a small environmental chamber and the Benzene Series was analyzed using GC-MS. The concentration of Benzene Series released by MDF was 316.24 μg·m−3, and that of the NC-lacquered MDF with thicknesses of 18 mm and 8 mm were 284.44 μg·m−3 and 281.06 μg·m−3, respectively. The MDF released 14 kinds of Benzene Series, and the NC-lacquered MDF with two thicknesses released 18 kinds of it. The release concentration order of Benzene Series in NC-lacquered MDF with different diluents of the 18 mm thick panel was NC-M, NC-A, and NC-E from high to low. The lowest concentration of it occurred when the ratio of anhydrous ethanol to ethyl acetate was 1:3, and the lowest amount of components were at the ratio of 1:2. The concentration of Benzene Series released by MDF is higher than that released by the NC-lacquered MDF. Thickness has no effect on the type of release. The thicker MDF was, the higher the concentration was. The alcohol and ester thinner can control the release of Benzene Series from the source, and the optimal mixing ratio was 1:3.

Lacquer

Diluent

Anhydrous

10.3390/atmos14010021

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Citations (3)