Bei Wang

Guangdong Ocean University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jichang Jian

Guangdong Ocean University

Yishan Lu

Guangdong Ocean University

Jufen Tang

Guangdong Ocean University

Jia Cai

Guangdong Ocean University

Zaohe Wu

Guangdong Ocean University

Yu Huang

Guangdong Ocean University

Zhongliang Wang

Guangdong Ocean University

Yuan Li

Guangdong Ocean University

Yucong Huang

Guangdong Ocean University

Jinzhong Niu

Guangdong Ocean University

Cooperative Institutions

Guangdong Ocean University

110

Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

Shanghai Jiao Tong University

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)

Qingdao National Laboratory for Marine Science and Technology

Sun Yat-sen University

Southwest University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Expression of sglt1 gene in Cyprinus carpio and preparation of its polyclonal antibody

JOURNAL OF FISHERIES OF CHINA (2012)

Guoxing Nie Bei Wang Xiao Yan Caixia Hou Zhang Jianxin

The high-affinity Na+/glucose contransporter Sglt1 is one of the important members of the sodium:solute symporter family(SSF),belonging to the homologous family 5(SLC5).The Sglt1 plays an important role in accumulating glucoses from intestinal or kidney epithelial cells against an adverse concentration gradient and maintaining the adjustment of metabolism.So far,few studies on Na+/glucose cotransporter have been reported in the freshwater fishes.This research would develop special antibodies of Sglt1,which could supply the foundation for the research of glucose metabolism in Cyprinus carpio intestines on molecular level.To study the molecular mechanism of glucose metabolism in freshwater fishes,the full-length cDNA of sglt1 in C.carpio with an ORF of 1 977 bp was cloned and the antigenicity of Sglt1 was first predicted.92(544-637)amino acids with strong antigenicity and immunogenicity were selected as target section of sglt1 in C.carpio and were cloned into plasmid pET-32a(+)vector which had restriction sites for EcoRⅠ and HindⅢ.The rcombinant plasmid named pET-32a(+)-sglt1-P was transformed into E.coli Rosetta.The Sglt1-P fusion protein of approximately 30 ku was highly expressed in E.coli Rosetta after being induced with IPTG.The purified fusion protein was used as antigen to immunize New Zealand Rabbits with ear margin veins by subcutaneous injection with power.The results of ELISA showed that the titer of the antiserum was about 1∶ 105.The Sglt1-P polyclonal antibody was used to determine the expression of Sglt1 protein in C.carpio intestines through immunohistochemical method.The result revealed that the antibody performed high affinity and specificity and could be applied to study the expression and locating of Sglt1 in the C.carpio.The Sglt1-P polyclonal antibody also could serve as an important research tool to study Sglt1 expression and transshipment activity of C.carpio.Meanwhile,the Sglt1-P polyclonal antibody could also be used to exploratively study the expression location and quantity of the Sglt1 transporters in other fishes.

Polyclonal antibodies

10.3724/sp.j.1231.2012.27810

Cite

Citations (1)

Molecular characterization and expression of ZAP-70 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus

Genes & Genomics (2015)

Zhen Gan Bei Wang Wei Zhou Yishan Lu Yanfei Zhang

Nile tilapia

10.1007/s13258-015-0372-4

Cite

Citations (15)

Cloning and expression analysis of nonspecific cytotoxic cell receptor 1 (Ls-NCCRP1) from red snapper (Lutjanus sanguineus)

Marine Genomics (2013)

Jia Cai Shina Wei Bei Wang Yucong Huang Jufen Tang

Cloning (programming)

10.1016/j.margen.2013.07.001

Cite

Citations (12)

HEALTH EVALUATION OF WETLANDS DISTRIBUTED ALONG URBAN-RURAL GRADIENTS BASED ON V-IBI

Applied Ecology and Environmental Research (2024)

Guishan Yang Z.L. QU Yong Wang Xiaobo Zhou Bei Wang

10.15666/aeer/2204_31633186

Cite

Citations (0)

Identification and Characterization of four Bacillus species from the intestine of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), their antagonistic role on common pathogenic bacteria, and effects on intestinal health

Fish & Shellfish Immunology (2024)

Kwaku Amoah Jia Cai Yü Huang Bei Wang Vicent Michael Shija

Epinephelus

Identification

Pathogenic bacteria

Bacillus (shape)

10.1016/j.fsi.2024.109795

Cite

Citations (3)

Immunomulatory activity of Tetracapsuloides bryosalmonae calreticulin: Selective inhibition of pro-inflammatory cytokine activity in rainbow trout, Oncorhynchus mykiss

Developmental & Comparative Immunology (2023)

Jason W. Holland Bei Wang Tiehui Wang Marc Faber Beatriz Abós

10.1016/j.dci.2023.104954

Cite

Citations (0)

MshK mutation reduces pathogenicity of Aeromonas veronii by modulating swimming ability, biofilm formation capacity, pili structure and virulence gene expression

Aquaculture (2024)

Jiahui Lu Jinming Wei Kemei Liu Bei Wang Liping Zhang

Aeromonas veronii

Pathogenicity island

10.1016/j.aquaculture.2024.741337

Cite

Citations (1)

Strategies to enhance tilapia immunity to improve their health in aquaculture

Reviews in Aquaculture (2022)

Bei Wang Kim D. Thompson Eakapol Wangkahart Jidapa Yamkasem Melba G. Bondad‐Reantaso

Abstract Various emerging and re‐emerging diseases, such as streptococcosis, tilapia lake virus disease and infectious spleen and kidney necrosis virus disease, have had a significant economic impact on the tilapia industry over the last decade. These diseases have resulted in high levels of morbidity and mortality, production losses and trade restrictions. In the absence of effective husbandry management, disease prevention strategies and appropriate biosecurity measures, infectious diseases will continue to challenge the sustainability of global tilapia aquaculture. Strategies used to reduce the impact of disease include feeding probiotic and prebiotic supplements to enhance tilapia health and to modulate the fish's gut microbiota to improve gut health. Herbal medicines and immunostimulants are also used to enhance immunity to disease, while vaccines are available, or are under development, for a number of economically important pathogens affecting tilapia culture. This review provides an overview of the benefits of enhancing the immune response of tilapia to improve their health, and in turn reduce the levels of pathogens within tilapia farming systems.

Biosecurity

Animal Husbandry

Aquaculture of tilapia

10.1111/raq.12731

Cite

Citations (55)

Biological characterisation, expression and functional analysis of non-specific cytotoxic cell receptor protein 1 in Nile tilapia (Oreochromis niloticus)

Fish & Shellfish Immunology (2020)

Yu Huang Xinchao Liu Jia Cai Jufen Tang Shuanghu Cai

Nile tilapia

10.1016/j.fsi.2020.05.048

Cite

Citations (11)

Characterization and functional analysis of a galectin‐related protein B from Nile tilapia involved in the immune response to bacterial infection

Journal of Fish Diseases (2020)

Jinzhong Niu Guoling Luo Xinchao Liu Yu Huang Jufen Tang

Abstract Galectin‐related protein is a kind of lectin without canonical activity that regulates cell adhesion and cell growth. In this study, a novel galectin‐related protein B ( OnGRPB ) was identified from Nile tilapia ( Oreochromis niloticus ). The open reading frame of OnGRPB was 438 bp and encoded a peptide of 145 amino acids. The deduced protein sequence of OnGRPB possessed a conserved carbohydrate recognition domain (CRD) with partial sugar binding sites (N‐R, V‐N and W‐E) and shared high identities with other fish GRPB proteins. The qRT‐PCR analysis found that OnGRPB was widely distributed in various tissues and monocyte/macrophages (Mo/MΦ) of healthy tilapia. After Streptococcus agalactiae infection, OnGRPB transcripts were significantly up‐regulated in liver, spleen, head kidney and Mo/MΦ. The recombinant OnGRPB protein (rOnGRPB) had the binding activity and agglutination ability to bacteria. Also, rOnGRPB could modulate antibacterial activity and inflammatory factor expression of Mo/MΦ. These data collectively indicate that OnGRPB plays roles in the immune response of Nile tilapia against bacterial infection.

Nile tilapia

Galectin

Agglutination (biology)

Edwardsiella tarda

10.1111/jfd.13273

Cite

Citations (7)