Wei Chen

The First Affiliated Hospital, Sun Yat-sen University

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Shengyun Fang

University of Maryland, Baltimore

Guanghui Wang

Soochow University

Yili Yang

Suzhou Industrial Park Institute of Services Outsourcing

Tong Yu

Qiqihar Medical University

Yongwang Zhong

University of Maryland, Baltimore

Chen‐Ying Liu

Shanghai Cancer Institute

Xiaodan Hou

Zhengzhou Central Hospital

Henry Q.X. Li

Crown College

Tingyu Wu

Taipei Medical University-Shuang Ho Hospital

Yuyang Huang

Harbin University of Commerce

Cooperative Institutions

Sun Yat-sen University

304

Chinese Academy of Sciences

224

Chinese Academy of Medical Sciences & Peking Union Medical College

155

Shanghai Jiao Tong University

146

The First Affiliated Hospital, Sun Yat-sen University

133

Peking University

130

Sichuan University

117

Huazhong University of Science and Technology

116

Zhejiang University

115

University of Chinese Academy of Sciences

113

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Screening of cytokines associated with ionizing radiation dose in serum by cytokine array

Jiefangjun yixue zazhi (2013)

Qiong Liu Ying He Shen Xianrong Linfang Mo Qingrong Wang

Objective　To identify the cytokine biomarker candidates in serum associated with ionizing radiation dose, and analyze the patterns of them by cytokine antibody array. Methods　Eighty male C57BL/6 mice were randomly divided into radiation group (n=60) and control group (n=20). Mice in radiation group were treated with 60Co total-body irradiation with the dose of 3, 5 and 10Gy, respectively (20 each dose group). Six hours after radiation, blood was obtained from the orbital sinus bleeding, and serums were collected. The serum cytokine concentration was analyzed by antibody array of 96 mouse cytokines, and cytokine biomarker candidates which were shown to be dose-responsive to ionizing radiation had been identified. Results　 Interestingly, the majority of the 96 mouse cytokines showed positive changes in amounts of cytokine. Following ionizing radiation exposure, only 44 cytokines changed at the expose dose of 3Gy, while 47 cytokines changed at the dose of 5Gy and 66 cytokines at the dose of 10Gy. Further analysis revealed that 14 cytokines were shown to be dose-responsive to ionizing radiation. Among those, 7 cytokines were shown up-regulated in a dose-dependent trend in response to ionizing radiation, and 7 cytokines were shown downregulated. Conclusion　These serum cytokine biomarkers which are sensitive to ionizing radiation may act in a dose-dependent manner, suggesting that the serum cytokine biomarker candidates can be used for estimating dose of ionizing radiation. DOI: 10.11855/j.issn.0577-7402.2013.10.008

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Research status on immunotherapy trials of gastric cancer

World Journal of Clinical Cases (2021)

Chao Liang Hengmiao Wu Weiming Yu Wei Chen

The breakthrough of immune checkpoint inhibitor (ICI) therapy has created extensive opportunities for cancer immunotherapy. Especially, the block of programmed death-1/programmed death ligand 1 (PD-L1) axis using ICIs has become a new therapeutic strategy to treat advanced gastric cancer (GC). However, in the past decade, single-arm and randomized trials for single-drug ICI therapy showed that the therapeutic effect was not satisfactory, including clinical trials for advanced GC. However, after selecting suitable predictive biomarkers and developing a combination of anti-angiogenic targeted drugs and other chemotherapeutic drugs, the objective response rate and progression-free survival of patients with gastric cancer were improved significantly. The United States Food and Drug Administration has approved treatment with pembrolizumab for patients with advanced GC with PD-L1 expression or microsatellite instability-high/mismatch repair deficiency. In this review, the updated data from the latest trial results of combination immunotherapy for GC are presented. Based on the outcome of combination therapy, we discuss its possible molecular mechanism and summarize effective predictive biomarkers. We also discuss possible problems stemming from results of other clinical trials of ICI treatment and propose other directions for ICI therapy.

Combination therapy

Microsatellite Instability

Cancer Immunotherapy

Targeted Therapy

10.12998/wjcc.v9.i21.5782

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Construction of Label-Free Sensor for Ribavirin Detection Based on Colloidal Crystal Array and Molecular Imprinting

SSRN Electronic Journal (2022)

Wei Chen Yi Sun Lirui Zhang Min Fu Kunhua Wang

Ribavirin is an important antiviral with demonstrated activity against coronaviruses, such as SARS and COVID-19. However, abuse of ribavirin will cause great environmental damage and threaten human health owing to its reproductive toxicity and teratogenicity. Therefore, an innovative detection method is demand for simple and sensitive detection of ribavirin. This work reports a label-free sensor for ribavirin detection by combination of colloidal crystal array and molecular imprinting. The imprinted colloidal crystal array (ICCA) was obtained by self-assemble of ribavirin imprinted spheres. Owing to the high ordered structure, the ICCA exhibited optical properties which changed upon binding ribavirin. The changes in reflectance wavelength enabled a fast and label-free detection of ribavirin between 5 to 60 mg/L. Moreover, the sensor showed excellent selectivity for ribavirin detection in human serum sample. Overall, the ICCA offers the advantages of high selectivity, label-free and easy operation, which may constitute an alternative approach for real-time detection of antivirals.

Molecular imprinting

Imprinting (psychology)

10.2139/ssrn.4182141

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A multi-omic analysis of orange-spotted grouper larvae infected with nervous necrosis virus identifies increased adhesion molecules and collagen synthesis in the persistent state

Fish & Shellfish Immunology (2020)

Hui Ge Kebing Lin Chen Zhou Qi Lin Ziping Zhang

Proteome

10.1016/j.fsi.2020.01.056

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Supplementary Fig.1 from Nuclear Export of Ubiquitinated Proteins Determines the Sensitivity of Colorectal Cancer to Proteasome Inhibitor

Tingyu Wu Wei Chen Yongwang Zhong Xiaodan Hou Shengyun Fang

The cell viability assay with different order of drug treatment.

Nuclear export signal

10.1158/1535-7163.22507986

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Data from Inhibition of Protein Phosphatase 2A Enhances Cytotoxicity and Accessibility of Chemotherapeutic Drugs to Hepatocellular Carcinomas

Xueli Bai Qi Zhang Long‐Yun Ye Qida Hu Qihan Fu

<div>AbstractHepatocellular carcinoma (HCC) is one of the most common and therapeutically challenging malignancies worldwide. For patients ineligible for “curative resection” or liver transplantation, chemotherapy is an important minimally effective option. Strategies for chemosensitization are urgently needed. Here, we report that LB-100, a serine/threonine protein phosphatase 2A (PP2A) inhibitor, enhances the cytotoxicity of chemotherapy for HCC in vitro and in vivo. We found that LB-100 significantly enhanced inhibition of HCC by doxorubicin and cisplatin in vitro and in vivo in a PP2A-dependent way, while having little inhibitory activity when used alone. LB-100 promoted vascular endothelial growth factor secretion and vasculogenic mimicry, associated with increased microvessel density and blood perfusion of tumor cell xenografts. LB-100 also enhanced paracellular endothelial permeability to Evans Blue dye and doxorubicin in vivo and in vitro, presumably by altering vascular endothelial–cadherin contact between cells. Changes in permeability and perfusion were accompanied by increased accumulation of doxorubicin in HCC xenografts but not in normal liver tissue. In conclusion, LB-100 enhances chemotherapy by interfering with DNA damage–induced defense mechanisms and by increasing angiogenesis and drug penetration into tumor cells. The induction of angiogenesis and vascular permeability of tumor xenografts by inhibition of PP2A may be a novel approach for enhancing the cytotoxic treatment of HCC and potentially other cancers. Mol Cancer Ther; 13(8); 2062–72. ©2014 AACR.</div>

10.1158/1535-7163.c.6536655.v1

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Data from A Novel Small Molecule Targets Androgen Receptor and Its Splice Variants in Castration-Resistant Prostate Cancer

Zhenyu Yang Dan Wang James K. Johnson Laura E. Pascal Keita Takubo

<div>AbstractReactivation of androgen receptor (AR) appears to be the major mechanism driving the resistance of castration-resistant prostate cancer (CRPC) to second-generation antiandrogens and involves AR overexpression, AR mutation, and/or expression of AR splice variants lacking ligand-binding domain. There is a need for novel small molecules targeting AR, particularly those also targeting AR splice variants such as ARv7. A high-throughput/high-content screen was previously reported that led to the discovery of a novel lead compound, 2-(((3,5-dimethylisoxazol-4-yl)methyl)thio)-1-(4-(2,3-dimethylphenyl)piperazin-1-yl)ethan-1-one (IMTPPE), capable of inhibiting nuclear AR level and activity in CRPC cells, including those resistant to enzalutamide. A novel analogue of IMTPPE, JJ-450, has been investigated with evidence for its direct and specific inhibition of AR transcriptional activity via a pulldown assay and RNA-sequencing analysis, PSA-based luciferase, qPCR, and chromatin immunoprecipitation assays, and xenograft tumor model 22Rv1. JJ-450 blocks AR recruitment to androgen-responsive elements and suppresses AR target gene expression. JJ-450 also inhibits ARv7 transcriptional activity and its target gene expression. Importantly, JJ-450 suppresses the growth of CRPC tumor xenografts, including ARv7-expressing 22Rv1. Collectively, these findings suggest JJ-450 represents a new class of AR antagonists with therapeutic potential for CRPC, including those resistant to enzalutamide.</div>

Enzalutamide

Chromatin immunoprecipitation

splice

Antiandrogens

10.1158/1535-7163.c.6542233.v1

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CRISPR/Cas12a and recombinase polymerase amplification-based rapid on-site nucleic acid detection of duck circovirus

Virology Journal (2024)

Qi-Zhang Liang Wei Chen Rong-Chang Liu Qiuling Fu Guanghua Fu

Duck circovirus (DuCV) infections commonly induce immunosuppression and secondary infections in ducks, resulting in significant economic losses in the duck breeding industry. Currently, effective vaccines and treatments for DuCV have been lacking. Therefore, rapid, specific, and sensitive detection methods are crucial for preventing and controlling DuCV. A lateral flow strip (LFS) detection method was developed using recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a). The RPA-CRISPR/Cas12a-LFS targeted the DuCV replication protein (Rep) and was operated at 37 ℃ and allowed for visual interpretation without requiring sophisticated equipment. The results revealed that the reaction time of RPA-CRISPR/Cas12a-LFS is only 45 min. This method achieved a low detection limit of 2.6 gene copies. Importantly, this method demonstrated high specificity and no cross-reactivity with six other avian viruses. In a study involving 97 waterfowl samples, the Rep RPA-CRISPR/Cas12a-LFS showed 100% consistency and agreement with real-time quantitative polymerase chain reaction. These findings underscored the potential of this user-friendly, rapid, sensitive, and accurate detection method for on-site DuCV detection.

Recombinase Polymerase Amplification

Applications of PCR

Circovirus

Multiple displacement amplification

10.1186/s12985-024-02577-7

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The roles of MAPKs in disease

Cell Research (2008)

Michael C. Lawrence Arif Jivan Chunli Shao Lingling Duan Daryl L. Goad

MAPK cascade

10.1038/cr.2008.37