Yin Zhang

Chengdu University

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Zhouping Wang

Jiangnan University

Imran Mahmood Khan

University of Nottingham Ningbo China

Yuan Liu

Shanghai Jiao Tong University

Xiaoze Dong

China National Center for Food Safety Risk Assessment

Shuo Qi

Nanjing Forestry University

Lin Yue

State Key Laboratory of Food Science and Technology

Cuixia Sun

Shanghai Jiao Tong University

Yapeng Fang

Shanghai Jiao Tong University

Wenli Wang

Chinese Academy of Agricultural Sciences

Yuhan Sun

ShanghaiTech University

Cooperative Institutions

Shanghai Jiao Tong University

Jiangnan University

State Key Laboratory of Food Science and Technology

Chinese Academy of Sciences

Chengdu University

Ministry of Agriculture and Rural Affairs

Sichuan University

Yangzhou University

Chinese Academy of Medical Sciences & Peking Union Medical College

University of Chinese Academy of Sciences

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Umami Taste Evaluation Based on a Novel Mouse Taste Receptor Cell-Based Biosensor

Yuxia Fan Weizhu Chen Ninglong Zhang Mingyang Li Yiwen Zhu

Umami, a taste sensation known for its savory and delicious properties, has garnered considerable attention from both consumers and the food industry. However, current understanding and evaluation of umami characteristics remain limited, presenting a long-standing issue. To address this challenge, we have developed a self-assembled biosensor based on matured taste receptor cells (TRCs), obtained through isolation and culture of taste stem cells. TRCs, as the recognition element, were mounted onto the surface of a glassy carbon electrode (GCE) treated with gold nanoparticles (AuNPs) and poly-L-lysine (PLL). Key parameters were optimized to ensure the optimal performance of the TRCs-based biosensor. AuNPs were deposited onto the GCE surface via 90 s electrochemical reduction. TRCs concentration of 107/mL and incubation time of 12 h was chosen by electrochemical characterization. Using this novel, rapid, and sensitive TRCs-based biosensor, we successfully detected L-monosodium glutamate (MSG) and other umami substances, demonstrating a good linear relationship within the range of 10-9 – 10-5 M between response signals and concentration of MSG stimuli. Our results provide insights into taste signal transduction mechanisms and suggest the potential for biomimetic sensors in intelligent perception applications.

Umami

Taste receptor

10.2139/ssrn.4427961

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Review of Rice–Fish–Duck Symbiosis System in China—One of the Globally Important Ingenious Agricultural Heritage Systems (GIAHS)

Sustainability (2023)

Yin Zhang Chunyun Guan Zhengyou Li Jun Luo Bo Ren

The Chinese rice–fish–duck symbiosis system is one of the globally important agricultural heritage systems, with a history of nearly a thousand years. It embodies the wisdom of the Chinese ancestors and has huge comprehensive value, which has extremely important references for the sustainable development of agriculture. The technological core for the rice–fish–duck symbiosis system lies in the selection of rice, fish, and duck varieties and the coupling of agricultural operation time. In the process of modern agriculture, many varieties with high comprehensive benefits have also been applied to the rice–fish–duck system, and the rice–fish–duck model has been further developed. What is worrying is that the transfer of rural labor, the promotion of modern agricultural technology, the low degree of marketization and industrialization, the weakening of cultural awareness, and the change in climate have posed huge threats and challenges to the traditional Chinese rice–fish–duck system. The protection and development of the system are urgently needed. Therefore, we recommend delimiting active protection areas, establishing scientific research bases, improving relevant systems, developing rural tourism markets, and innovating farming technology, which also provide guidance for protecting and developing other agricultural cultural heritages.

Industrialisation

Promotion (chess)

10.3390/su15031910

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Citations (15)

Supplementary Table 1 from An Antimesothelin-Monomethyl Auristatin E Conjugate with Potent Antitumor Activity in Ovarian, Pancreatic, and Mesothelioma Models

Suzie J. Scales Nidhi Gupta Glenn Pacheco Ron Firestein Dorothy French

<p>Supplementary Table 1: Summary of 5B2 anti-mesothelin IHC literature and TMA details.</p>

Mesothelin

Conjugate

Table (database)

10.1158/1535-7163.22501776

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Colorimetric aptasensor for fumonisin B1 detection based on the DNA tetrahedra-functionalized magnetic beads and DNA hydrogel-coated bimetallic MOFzyme

Journal of Hazardous Materials (2022)

Yuhan Sun Shuo Qi Xiaoze Dong Mingwei Qin Ning Ding

Bimetallic strip

10.1016/j.jhazmat.2022.130252

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Citations (10)

Bamboo Field

Springer tracts in civil engineering (2022)

Qing Gong Yin Zhang Ruonan Qin Kewei Liu

Rattan

Ball (mathematics)

10.1007/978-3-030-91990-0_12

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A Visual Method of Hydroponic Lettuces Height and Leaves Expansion Size Measurement for Intelligent Harvesting

Agronomy (2023)

Yidong Ma Yin Zhang Xin Jin Xinping Li Huankun Wang

Harvesting is an important procedure for hydroponic lettuces in plant factories. At present, hydroponic lettuces are mainly harvested manually, and the key difficulty in mechanical harvesting is reducing the occurrence of leaf injury. Measuring the size of hydroponic lettuces using the image processing method and intelligently adjusting the operating parameters of the harvesting device are the foundation of high-quality harvesting for lettuces. The overlapped leaves of adjacent hydroponic lettuces cause difficulties in measuring lettuce size, especially the leaves expansion size. Therefore, we proposed an image processing method for measuring lettuce height and leaves expansion size according to the upper contour feature of lettuces and an image included three lettuces. Firstly, the upper contours of the lettuces were extracted and segmented via image preprocessing. Secondly, lettuce height was measured according to the maximum ordinate of the contour. Lastly, the lettuce’s upper contour was fitted to a function to measure the leaves expansion size. The measurement results showed that the maximal relative error of the lettuce height measurements was 5.58%, and the average was 2.14%. The effect of the quadratic function in fitting the upper contour was the best compared with the cubic function and sine function. The maximal relative error of the leaves expansion size measurements was 8.59%, and the average was 4.03%. According to the results of the lettuce height and leaves expansion size measurements, the grabbing parameters of each lettuce were intelligently adjusted to verify the harvesting effect. The harvesting success rates of lettuces was above 90%, and the injured leaves areas of the left, middle, and right lettuces in each image were 192.6 mm2, 228.1 mm2, and 205.6 mm2, respectively. This paper provides a reference for the design and improvement of intelligent harvesters for hydroponic lettuces.

10.3390/agronomy13081996

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Noteworthy Consensus Effects of D/E Residues in Umami Peptides Used for Designing the Novel Umami Peptides

Journal of Agricultural and Food Chemistry (2024)

Zhiyong Cui Hengli Meng Tianxing Zhou Yanyang Yu Jiaming Gu

Aspartic acid (D) and glutamic acid (E) play vital roles in the umami peptides. To understand their exact mechanism of action, umami peptides were collected and cut into 1/2/3/4 fragments. Connecting D/E to the N/C-termini of the fragments formed D/E consensus effect groups (DEEGs), and all fragments containing DEEG were summarized according to the ratio and ranking obtained in the above four situations. The interaction patterns between peptides in DEEG and T1R1/T1R3-VFD were compared by statistical analysis and molecular docking, and the most conservative contacts were found to be HdB_277_ARG and HdB_148_SER. The molecular docking score of the effector peptides significantly dropped compared to that of their original peptides (−1.076 ± 0.658 kcal/mol, p value < 0.05). Six types of consensus fingerprints were set according to the Top7 contacts. The exponential of relative umami was linearly correlated with ΔGbind (R2 = 0.961). Under the D/E consensus effect, the electrostatic effect of the umami peptide was improved, and the energy gap between the highest occupied molecular orbital–the least unoccupied molecular orbital (HOMO–LUMO) was decreased. The shortest path map showed that the peptides had similar T1R1–T1R3 recognition pathways. This study helps to reveal umami perception rules and provides support for the efficient screening of umami peptides based on the material richness in D/E sequences.

Umami

10.1021/acs.jafc.3c07026

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Citations (13)

Cobaltaelectro-Catalyzed Oxidative C–H/N–H Activation with 1,3-Diynes by Electro-Removable Hydrazides

Organic Letters (2019)

Ruhuai Mei Wenbo Ma Yin Zhang Xiaoqiang Guo Lutz Ackermann

An efficient electro-oxidative C–H/N–H activation with 1,3-diynes has been achieved with a robust earth-abundant cobalt catalyst. The electrochemical C–H functionalization was accomplished with ample scope and remarkable functional group compatibility in a simple undivided cell. This protocol avoids the utilization of stoichiometric and cost-intensive chemical oxidants in C–H activation, thus forming hydrogen as the only byproduct.

10.1021/acs.orglett.9b02463

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Citations (83)

Physical dormancy and soil seed bank dynamics in seeds of Melilotus albus (Fabaceae)

Flora (2020)

Yin Zhang Keliang Zhang Yusong Ji Jun Tao

Scarification

Soil seed bank

Melilotus

10.1016/j.flora.2020.151600

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Citations (11)

Preparation, characterization and bioavailability of oral puerarin nanoparticles by emulsion solvent evaporation method

RSC Advances (2016)

Yin Zhang Yong Li Xiuhua Zhao Yuangang Zu Weiguo Wang

In this paper, puerarin (PUE) was nanocrystallized by emulsion solvent evaporation (ESE) method, followed by freeze-drying. The solubility, dissolution rate and oral bioavailability of PUENs were significantly improved compared with raw PUE.

Puerarin

10.1039/c6ra08413c

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Citations (20)