Xuan Zhang

Army Medical University

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Jianye Zang

University of Science and Technology of China

Chengliang Wang

UConn Health

Minhao Wu

Sun Yat-sen University

Wanbiao Chen

Chinese Academy of Sciences

Tianrong Hang

Anhui University

Xuebiao Yao

University of Science and Technology of China

Yang Pan

University of Science and Technology of China

Xi Mo

Shanghai Children's Medical Center

Keke Qi

National Synchrotron Radiation Laboratory

Xiaobao Chen

Fujian Medical University

Cooperative Institutions

Chinese Academy of Sciences

University of Science and Technology of China

Hefei National Center for Physical Sciences at Nanoscale

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking University

Fudan University

Sun Yat-sen University

Guangzhou Medical University

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Structural insights into the intermolecular interaction of the adhesin SdrC in the pathogenicity of Staphylococcus aureus

Acta Crystallographica Section F Structural Biology Communications (2021)

Junchao Wang Min Zhang Mingzhu Wang Jianye Zang Xuan Zhang

Staphylococcus aureus is an opportunistic disease-causing pathogen that is widely found in the community and on medical equipment. A series of virulence factors secreted by S. aureus can trigger severe diseases such as sepsis, endocarditis and toxic shock, and thus have a great impact on human health. The transformation of S. aureus from a colonization state to a pathogenic state during its life cycle is intimately associated with the initiation of bacterial aggregation and biofilm accumulation. SdrC, an S. aureus surface protein, can act as an adhesin to promote cell attachment and aggregation by an unknown mechanism. Here, structural studies demonstrate that SdrC forms a unique dimer through intermolecular interaction. It is proposed that the dimerization of SdrC enhances the efficiency of bacteria–host attachment and therefore contributes to the pathogenicity of S. aureus .

10.1107/s2053230x21000741

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Citations (6)

Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1

Biochemical Journal (2010)

Xuan Zhang Y.B. Teng Jianping Liu Yong‐Xing He Kang Zhou

In most eubacteria, fungi, apicomplexa, plants and some metazoans, the active form of vitamin B6, PLP (pyridoxal 5-phosphate), is de novo synthesized from three substrates, R5P (ribose 5-phosphate), DHAP (dihydroxyacetone phosphate) and ammonia hydrolysed from glutamine by a complexed glutaminase. Of the three active sites of DXP (deoxyxylulose 5-phosphate)independent PLP synthase (Pdx1), the R5P isomerization site has been assigned, but the sites for DHAP isomerization and PLP formation remain unknown. In the present study, we present the crystal structures of yeast Pdx1/Snz1, in apo-, G3P (glyceraldehyde 3-phosphate)- and PLP-bound forms, at 2.3, 1.8 and 2.2 Å (1 Å=0.1 nm) respectively. Structural and biochemical analysis enabled us to assign the PLP-formation site, a G3P-binding site and a G3P-transfer site. We propose a putative catalytic mechanism for Pdx1/Snz1 in which R5P and DHAP are isomerized at two distinct sites and transferred along well-defined routes to a final destination for PLP synthesis.

Dihydroxyacetone phosphate

DHAP

Glutamine amidotransferase

10.1042/bj20101241

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Citations (19)

SRC-1 Knockout Exerts No Effect on Amyloid β Deposition in APP/PS1 Mice

Frontiers in Aging Neuroscience (2020)

Qiong Wu Bin Wang Qifa Li Xuan Zhang Michael Ntim

Steroid receptor coactivator 1 (SRC-1) is the key coactivator because of its transcriptional activity. Previous studies have shown that SRC-1 is abundant in the hippocampus and has been implicated in cognition. SRC-1 is also related to some major risk factors for Alzheimer's disease (AD), such as a decline in estrogen and aging, however, whether SRC-1 is involved in the pathogenesis of AD remains unclear. In this study, we established SRC-1 knockout in AD mice by cross breeding SRC-1-/- mutant mice with APP/PS1 transgenic mice, and investigated the expression of some synaptic proteins, the amyloid β (Aβ) deposition, and activation of astrocytes and microglia in the hippocampus of APP/PS1×SRC-1-/- mice. The results showed that SRC-1 knockout neither affects the Aβ plaque and activation of glia, nor changes the expression of synaptic proteins in AD model mice. The above results suggest that the complete deletion of SRC-1 in the embryo exerts no effect on the pathogenesis of APP/PS1 mice. Nevertheless, this study could not eliminate the possible role of SRC-1 in the development of AD due to the lack of observation of other events in AD such as tau hyperphosphorylation and the limitation of the animal model employed.

Knockout mouse

Hyperphosphorylation

Pathogenesis

Gene knockout

10.3389/fnagi.2020.00145

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Citations (4)

Zeylleucapenoids A–D, Highly Oxygenated Diterpenoids with Anti-Inflammatory Activity from Leucas zeylanica (L.) R. Br.

Molecules (2023)

Ting Zhao Xuan Zhang Xu‐Hua Nong Xue‐Ming Zhou Ru-Ru Chai

Four previously undescribed highly oxygenated diterpenoids (1-4), zeylleucapenoids A-D, characterized by halimane and labdane skeletons, were isolated from the aerial parts of Leucas zeylanica. Their structures were elucidated primarily via NMR experiments. The absolute configuration of 1 was established using theoretical ECD calculations and X-ray crystallographic analysis, whereas those for 2-4 were assigned using theoretical ORD calculations. Zeylleucapenoids A-D were tested for anti-inflammatory activity against nitric oxide (NO) production in RAW264.7 macrophages, of which only 4 showed significant efficacy with an IC50 value of 38.45 μM. Further, active compound 4 was also evaluated for the inhibition of the release of pro-inflammatory cytokines TNF-α and IL-6 and was found to have a dose-dependent inhibitory effect, while it showed nontoxic activity for zebrafish embryos. A subsequent Western blotting experiment revealed that 4 inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, molecular docking analysis indicated that the possible mechanism of action for 4 may be bind to targets via hydrogen and hydrophobic bond interactions.

Labdane

Proinflammatory cytokine

Docking (animal)

IC50

Anti-inflammatory

10.3390/molecules28114472

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Citations (1)

Enhancing hemoglobin peptide production from chicken blood fermentation by food‐grade nonionic surfactant

Biotechnology and Applied Biochemistry (2019)

Deng‐Long Li Bin Wu Xuan Zhang Mingyuan Li Jingrong Cheng

This study is focused on employing a potential process technology for enhancing hemoglobin peptides production from chicken blood. Effects of surfactants on chicken blood biodegradation and hemoglobin polypeptide accumulation were evaluated and the bioconversion conditions were optimized. Results suggested that surfactants exhibited the positive effect on hemoglobin peptides production during chicken blood bioconversion by Aspergillus niger. Dodecyl glucopyranoside was selected as the optimal surfactant and added at the 48th hour of the fermentation process (64 H) at the concentration of 6.0 g/L. Under the optimized conditions, 104.5 mg·N/mL amino nitrogen, 638.3 mg·N/mL nonprotein nitrogen, and 766.3 mg·N/mL soluble nitrogen were detected, which increased by approximately 0.7-, 3.7-, and 3.8-fold, respectively, compared with the control. Furthermore, the acid protease stability was remarkably intensified and the accumulated peptides were mainly distributed at 500-2,000 Da. Results from this work corroborate the potential of applying dodecyl glucopyranoside in hemoglobin polypeptide production from chicken blood.

Nonionic surfactant

10.1002/bab.1795

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Citations (2)

Structural insights into the recognition of histone H3Q5 serotonylation by WDR5

Science Advances (2021)

Jie Zhao Wanbiao Chen Yi Pan Yinfeng Zhang Huiying Sun

WDR5 specifically recognizes histone H3Q5ser to promote gene transcription and neuroblastoma cell proliferation.

10.1126/sciadv.abf4291

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Citations (27)

Online Container Caching with Late-Warm for IoT Data Processing